We studied the cytokine profile (IL-1α,
IL-1β, IL-2, IL-4, IL-6, IL-10,
GM-CSF, interferon-γ, TNFα,) of the lymph, blood serum and
conditioned medium of the lymph cells of Wistar rats. It was found that the
concentration of cytokines investigated in the lymph of Wistar rats was significantly
higher compared to the serum. Age and gender differences between the
concentration of pro- and anti-inflammatory cytokines in the lymph and blood
serum were found. The concentration of the cytokines was determined using the
test system “Bio-Rad”.
Cite this paper
Poveshchenko, A. , Orlov, N. , Kazakov, O. , Poveshchenko, O. , Kim, I. , Bondarenko, N. , Miller, T. , Strunkin, D. , Kabakov, A. , Reiter, T. and Konenkov, V. (2014) Age and Gender Differences in Cytokine Profile of Lymph and Blood Serum. Advances in Aging Research
, 216-221. doi: 10.4236/aar.2014.33030
 Poveshchenko, O., Kolesnikov, A., Kim, I., Ulyanov, E., Mozzherina, A., Jankajte, E., Solovieva, A., Herter, T., Poveshchenko, A. and Konenkov, V. ( 2008 ) Methods for Isolating and Culturing Conditions Mesenchymal Stromal Cells from Human Adipose Tissue Obtained from Various Sources. Bulletin of RAMS, 133, 90-95.
 Poveshchenko, A., Abramov, V. and Kozlov, V. (2007) Cytokines—Factors Neuroendocrine Regulation. Advances of Physiological Sciences, 3, 40-46.
 Kuznetsov, A. (1993) A New Way of Sampling Lymph Animals. Bulletin of Experimental Biology and Medicin, 116, 329-331.
 Xin, D., Harris, M., Wade, K., Amin, M., Barr, A. and Barbe, M. (2011) Aging Enhances Serum Cytokine Response but Not Task-Induced Grip Strength Declines in a Rat Model of Work-Related Musculoskeletal Disorders. BMC Musculoskeletal Disorders, 12, 63. http://dx.doi.org/10.1186/1471-2474-12-63
 Forsey, R., Thompson, J., Ernerudh, J., et al. (2003) Plasma Cytokine Profiles in Elderly Humans. Mechanisms of Ageing and Development, 124, 487-493. http://dx.doi.org/10.1016/S0047-6374(03)00025-3
 Cecchini, G., Dominguez, M., Mocci, S., Wetterwald, A., Felix, R., Fleisch, H., Chisholm, O., Hofstetter, W., Pollard, J. and Stanley, R. (1994) Role of Colony Stimulating Factor-1 in the Establishment and Regulation of Tissue Macrophages during Postnatal Development of the Mouse. Development, 120, 1357-1372.
 Kozlov, V., Poveshchenko, A. and Gromykhina, N. (1990) Some Mechanisms Involved in the Prostaglandin E2 Immunosuppressive Effect in (CBA X C57BL) F1 Mice in Vivo. Cellular Immunology, 128, 242-249. http://dx.doi.org/10.1016/0008-8749(90)90022-J
 Rink, L., Cakman, I. and Kirchner H. (1998) Altered Cytokine Production in the Elderly. Mechanisms of Ageing and Development, 102, 199-209. http://dx.doi.org/10.1016/S0047-6374(97)00153-X