AS  Vol.5 No.8 , July 2014
Physiological Role of Humic Acid and Nicotinamide on Improving Plant Growth, Yield, and Mineral Nutrient of Wheat (Triticum durum) Grown under Newly Reclaimed Sandy Soil

Humic acid is not a fertilizer as it does not directly provide nutrients to plants, but is a compliment to fertilizer. Nicotinamide (Vitamin pp) is a stress-associated compound that can induce and regulate secondary metabolic accumulation and/or the manifestation of defense metabolism in plants. A field experiment was conducted at the experimental station of National Research center at El-Nubaria region, Egypt, to study the role of foliar application of humic acid (as soil conditioner 13 cm/l) and/or priming grains in nicotinamide (vitamin pp 5 mg/l) in saving irrigation water, decreasing fertilizer dose of NPK and at the same time increasing durum wheat (Triticum durum) cultivars’ (Beni Sweif-1 and Beni Sweif-3) productivity grown under newly reclaimed sandy soil exposed to drought for three weeks continuously. The results showed that plant treated with humic acid or nicotinamide increased significantly all morphological criteria (plant height, leaves number, fresh and dry weights of shoots), metabolism (photosynthetic pigment, total soluble sugar, total carbohydrates, total amino acids and proline), mineral contents (N, P, K, Ca and Mg) and yield (grain, straw and biology) of both cultivars amended with either recommended or half recommended doses of NPK. Foliar application of humic acid to plant priming in Vitamin pp induced significant increases in all studied parameters (morphology, chemical and yield) of plants amended with recommended or half recommended doses of NPK as compared with control plants. The maximum yields of grain, straw and biology of both cultivars were obtained in response to triple treatment (humic acid + nicotinamide + recommended dose of NPK) or (humic acid + nicotinamide + half recommended dose of NPK) respectively.

Cite this paper
El-Bassiouny, H. , Bakry, B. , El-Monem Attia, A. and Abd Allah, M. (2014) Physiological Role of Humic Acid and Nicotinamide on Improving Plant Growth, Yield, and Mineral Nutrient of Wheat (Triticum durum) Grown under Newly Reclaimed Sandy Soil. Agricultural Sciences, 5, 687-700. doi: 10.4236/as.2014.58072.
[1]   Jaleel, C.A., Sankar, B., Murali, P.V., Gomathinayagam, M., Lakshmananm, G.M.A. and Panneerselvam, R. (2008) Water Deficit Stress Effects on Reactive Oxygen Metabolism in Catharanthus Roseus; Impacts on Ajmalicine Accumulation. Colloids and Surfaces B: Biointerfaces, 62, 105-111.

[2]   Ayus, M., Moreno, J.L., Hernández, T. and Garcia, C. (1999) Characterisation and Evaluation of Humic Acids Extracted from Urban Waste as Liquid Fertilizers. European Journal of Soil Science, 75, I4481-I4488.

[3]   Azarpour, E., Moraditochaee, M. and Bozorgi, H.R. (2012) Evaluating Energy Balance and Energy Indices of Wheat Production in Rain-Fed Farming in Northern Iran. African Journal of Agricultural Research, 7, 1950-1955.

[4]   Chen, Y. and Aviad, T. (1990) Effect of Humic Substances on Plant Growth. In: Maccarthy, P., Ed., Humic Substances in Soil and Crop Sciences: Selected Readings. American Society of Agronomy and Soil Sciences, Madison, 161-186.

[5]   Tan, K.H. (2003) Chemical Composition of Humic Acidmatter. In: Humic Acids in Soil and the Environment, Principles and Controversies, Marcel and Dekker, New York.

[6]   Ferrara, G. and Brunetti, G. (2010) Effects of the Times of Application of a Soil Humic Acid on Berry Quality of Table Grape (Vitis vinifera L.) c.v Italia. Spanish Journal of Agricultural Research, 8, 817-822.

[7]   Nardi, S., Pizzeghello, D., Muscolo, A. and Vianello, A. (2002) Physiological Effects of Humic Substances on Higher Plants. Soil Biology and Biochemistry, 34, 1527-1536.

[8]   Liu, C. and Cooper, R.J. (2000) Humic Substances Influence Creeping Bentgrass Growth. Golf Course Management, 49-53.

[9]   Anonymous (2010) Humic and Fulvic Acids.

[10]   Berglund, T. and Ohlsson, A.B. (1995) Defensive and Secondary Metabolism in Plant Tissue Cultures, with Special Reference to Nicotinamide, Glutathione and Oxidative Stress. Plant Cell, Tissue and Organ Culture, 43, 137-145.

[11]   Chapman, H.O. and Pratt, P.E. (1978) Methods of Analysis for Soils, Plants and Water. Division of Agriculture Sciences University California, Berkley, 5-6.

[12]   Lichtenthaler, H.K. and Buschmann, C. (2001) Chlorophylls and Carotenoids: Measurement and Characterization by UV-VIS Spectroscopy. In: Wrolstad, R.E., Acree, T.E., An, H., Decker, E.A., Penner, M.H., Reid, D.S., Schwartz, S.J., Shoemaker, C.F. and Sporns, P., Eds., Current Protocols in Food Analytical Chemistry (CPFA), John Wiley and Sons, New York, F4.3.1-F4.3.8.

[13]   Larsen, P., Harbo, A., Klungron, S. and Ashein, T.A. (1962) On the Biosynthesis of Some Indole Compounds in Acetobacter Xylinum. Physiologia Plantarum, 15, 552-565.

[14]   Danil, A.D. and George, C.M. (1972) Peach Seed Dormancy in Relation to Endogenous Inhibitors and Applied Growth Substances. Journal of the American Society for Horticultural Science, 17, 621-624.

[15]   Homme, P.M., Conalez, B. and Billard, J. (1992) Carbohydrate Content, Fructose and Sucrose, Enzyme Activities in Roots, Stubble and Leaves of Rye Grass (Lolium perenne L.) as Affected by Source/Sink Modification after Cutting. Journal of Plant Physiology, 140, 282-291.

[16]   Yemm, E.W. and Willis, A.J. (1954) The Respiration of Barley Plants. IX. The Metabolism of Roots during Assimilation of Nitrogen. New Phytologist, 55, 229-234.

[17]   Herbert, D., Phipps, P.J. and Strange, R.E. (1971) Chemical Analysis of Microbial Cells. Methods in Microbiology, 5, 209-344.

[18]   Smith, F., Gilles, M.A., Hamilton, J.K. and Godees, P.A. (1956) Colorimetric Method for Determination of Sugar Related Substances. Analytical Chemistry, 28, 350-356.

[19]   Bates, L.S., Waldren, R.P. and Reare, I.D. (1973) Rapid Determination of Free Proline for Water Stress Studies. Plant and Soil, 39, 205-207.

[20]   Vartainan, N., Hervochon, P., Marcotte, L. and Larher, F. (1992) Proline Accumulation during Drought Rhizogenesis in Brassica napus var. Oleifera. Journal of Plant Physiology, 140, 623-628.

[21]   Yemm, E.W., Cocking, E.C. and Ricketts, R.E. (1955) The Determination of Amino Acids with Ninhydrin. Analyst, 80, 209-214.

[22]   Badford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein Dye Binding. Analytical Biochemistry, 72, 248-254.

[23]   Snedecor, G.W. and Cochran, W.G. (1980) Statistical Methods. 7th Edition, the Iowa State University Press, Ames, IA.

[24]   Duncan, D.B. (1955) Multiple Range and Multiple F-Test. Biometrics, 11, 1-42.

[25]   Huang, P.M., Wang, M.K. and Chiu, C.H. (2005) Soil Mineral-Organic Matter-Microbe Interactions: Impact on Biogeochemical Processes and Biodiversity in Soils. Pedobiologia, 49, 609-635.

[26]   von Lutzow, M., Koegel-Knabner, I., Eckschmitt, E., Matzner, E., Guggenberger, G., Marschner, B. and Flessa, H. (2006) Stabilization of Organic Matter in Temperate Soils: Mechanism and Their Relevance under Different Soil Conditions. European Journal of Soil Science, 57, 426-445.

[27]   Patil, R. (2010) Effect of Potassium Humate and Deproteinised Juice (DPJ) on Seed Germination and Seedling Growth of Wheat and Jowar. Annals of Biological Research, 1, 148-151.

[28]   Peymaninia, Y., Valizadeh, M., Shahryari, R., Ahmadizadeh, M. and Habibpour, M. (2012) Relationship among Morpho-Physiological Traits in Bread Wheat against Drought Stress at Presence of a Leonardite Derived Humic Fertilizer under Greenhouse Condition. International Research Journal of Applied and Basic Science, 3, 822-830.

[29]   Hassanein, R.A., Bassony, F.M., Barakat, D.M. and Khalil, R.R. (2009) Physiological Effects of Nicotinamide and Ascorbic Acid on Zea mays Plant Grown under Salinity Stress. 1-Changes in Growth, Some Relevant Metabolic Activities and Oxidative Defense Systems. Research Journal of Agriculture and Biological Sciences, 5, 72-81.

[30]   El-Bassiony, H.M. (2005) Physiological Responses of Wheat to Salinity Alleviation by Nicotinamide and Tryptophan. Internationa Journal of Agriculture and Biology, 7, 653-659.

[31]   Ferrara, G. and Brunetti, G. (2008) Foliar Applications of Humic Acids in Vitis vinifera L. cv Italia. J Int Sci Vigne Vin, 42, 79-87.

[32]   Ameri, A. and Tehranifar, A. (2012) Effect of Humic Acid on Nutrient Uptake and Physiological Characteristic Fragaria x Ananassa Var: Camarosa. Journal of Biological Environment Science, 6, 77-79.

[33]   Hathout, T.A., Shetawi, S.A. and Khallal, S.M. (1993) Effect of Mode of Application of Some Growth Regulators on the Physiology of Tomato Plants. III. Effect of Nicotinamide on Morphology Growth, Metabolism and Productivity. Egyptian Journal of Physiological Sciences, 17, 183-200.

[34]   Taylor, S.E., Terry, N. and Huston, R.P. (1982) Limiting Factors in Photosynthesis. Plant Physiology, 10, 1541-1543.

[35]   Kalaichelvi, K., Chinnusamy, C. and Arul, S.A. (2006) Exploiting the Natural Resourse-Lignite Humic Acid in Agriculture. Agricultural Reviews, 27, 276-283.

[36]   Pokorny, J. (2001) Introduction. In: Pokorny, J., Yanishlieva, N. and Gordon, M., Eds., Antioxidants in Food, Practical Applications, Woodhead Publishing Limited, Cambridge, 1-3.

[37]   Lewis, N. and Yamamoto, E. (1990) Lignin: Occurrence, Biogenesis and Biodegradation. Annual Review of Plant Physiology and Plant Molecular Biology, 41, 455-496.

[38]   Khattab, H. (2007) Role of Glutathione and Polyadenylic Acid on the Oxidative Defense Systems of Two Different Cultivars of Canola Seedlings Grown under Saline Condition. Australian Journal of Basic and Applied Sciences, 1, 323-334.

[39]   Unlu, H., Unlu, H.O. and Karakurt, Y. (2010) Influence of Humic Acid on the Antioxidant Compounds in Pepper Fruit. Food, Agriculture and Environment, 8, 434-438.

[40]   Serenella, N., Pizzeghelloa, D.A., Muscolob, N. and Vianello, A. (2002) Physiological Effects of Humic Substances on Higher Plants. Soil Biology and Biochemistry, 34, 1527-1536.

[41]   Merlo, L., Ghisi, R., Rascio, N. and Passera, C. (1991) Effects of Humic Substances on Carbohydrate Metabolism of Maize Leaves. Canadian Journal of Plant Science, 71, 419-425.

[42]   Kodandaramaiah, J., Ven Katoramaiah, C., Gopala Rao, P. and Rao, K.N. (1984) Effect of Bgroup Vitamins on the Endogenous Cytokinin Levels of Clusterbeans Cyamopsis tetragonoloba L. Taub. Proceedings of the National Academy of Sciences (India), 54, 95-98.

[43]   Farahat, M.M., Mazhar, A.A.M., Mona, H. and Mahgoub, M.H. (2012) Response of Khaya senegalensis Seedlings to Irrigation Intervals and Foliar Application of Humic Acid. Journal of Horticultural Science & Ornamental Plants, 4, 292-298.

[44]   Delavari, P.M., Baghizadeh, A., Enteshari, S.H., Kalantari, K.H.M., Yazdanpanah, A. and Mousavi, E.A. (2010) The Effects of Salicylic Acid on Some of Biochemical and Morphological Characteristic of Ocimum basilicucm under Salinity Stress. Australian Journal of Basic and Applied Sciences, 4, 4832-4845.

[45]   Gzik, A. (1996) Accumulation of Proline and Pattern of α-Amino Acids in Sugar Beet Plants in Response to Osmotic, Water and Salt Stress. Environmental and Experimental Botany, 36, 29-38.

[46]   Bandurska, H. (1993) In Vitro and in Vivo Effect of Proline on Nitrate Reductase Activity under Osmotic Stress in Barley. Acta Physiologiae Plantarum, 15, 83-88.

[47]   Harinasut, P., Srisunak, S., Pitukchaisopol, S. and Charoensataporn, R. (2000) Mechanisms of Adaptation to Increasing Salinity of Mulberry: Proline Content and Ascorbate Peroxidase Activity in Leaves of Multiple Shoots. ScienceAsia, 26, 207-211.

[48]   Maggio, A., Migazaki, S.P., Veronese, T., Fujita, H.I., Ibeas, B., Damsz, M.L., Navasimhan, P.M., Joly, R.A. and Bressan, R.A. (2002) Does Proline Accumulation Play an Active Role in Stress Induced Growth Reduction? Plant Journal, 31, 699-712.

[49]   Khaled, H. and Fawy, H.A. (2011) Effect of Different Levels of Humic Acids on the Nutrient Content, Plant Growth, and Soil Properties under Conditions of Salinity. Soil & Water Research, 6, 21-29.

[50]   Erik, B.G., Feiber, C., Shock, G. and Saundres, L.D. (2000) Evaluation of Humic Acid and Other Nonconventional Fertilizer Additiones for Onion Production. Malheur Experiment Station Oregon State University, Ontario, OR.

[51]   Mahgoub, G.M.A., Khedr, E.A.F., Amer, S.M. and Faisal, R.I.I. (1991) Effect of Adenosine Triphosphate (ATP) and Nicotinamide Adenine Dinucleotide (NAD) on Maize Grain Yield and N-P-K Uptake in Grains. Annals of Agricultural Science, Moshtohor, 29, 631-644.

[52]   Bozorgi, H.R., Pendashteh, M., Tarighi, F., Ziaei Doustan, H., Keshavarz, A.K., Azarpour, E. and Moradi, M. (2011) Effect of Foliar Zinc Spraying and Nitrogen Fertilization on Seed Yield and Several Attributes of Groundnut (Arachis hypogaea L.). World Applied Sciences Journal, 13, 1209-1217.

[53]   Taiz, L. and Zeiger, E. (1998) Plant Physiology. Sinaur Associates Inc., Sunderland.