JBNB  Vol.5 No.3 , July 2014
Comparative Study of Synergistic Effects of Antibiotics with Triangular Shaped Silver Nanoparticles, Synthesized Using UV-Light Irradiation, on Staphylococcus aureus and Pseudomonas aeruginosa
Abstract: In the present work, a comparative study of antibacterial activity and synergistic effects of triangular silver nanoparticles in combination with two standard antibiotics is discussed for Staphylococcus aureus and Pseudomonas aeruginosa. A green route was developed to synthesise silver nanoparticles in which silver oxalate was taken as precursor, black tea leaves extract as surfactant and chitin as a stabilizing agent. A grey coloured colloidal solution of silver nanoparticles was obtained which was characterized by using various techniques like X-ray diffractometer (XRD), transmission electron microscopy (TEM) and UV-visible spectroscopy. Anti-bacterial studies gave approximately equal inhibition zones for both the combinations which states that silver nanoparticles are to be equally effective and synergistic effects were clearly observed in case of P. aeruginosa.
Cite this paper: Saha, S. , Malik, M. and Qureshi, M. (2014) Comparative Study of Synergistic Effects of Antibiotics with Triangular Shaped Silver Nanoparticles, Synthesized Using UV-Light Irradiation, on Staphylococcus aureus and Pseudomonas aeruginosa. Journal of Biomaterials and Nanobiotechnology, 5, 186-193. doi: 10.4236/jbnb.2014.53022.

[1]   Knoll, B. and Keilmann, F. (1999) Near Field Probing of Vibrational Absorption for Chemical Microscopy. Nature, 399, 134-137.

[2]   Sengupta, S., Eavarone, D., Capila, I., Zhao, G.L., Watson, N., Kiziltepe, T., et al. (2005) Temporal Targeting of Tumor Cells and Neovasculature with a Nanoscale Delivery System. Nature, 436, 568-572.

[3]   Wiley, B., Sun, Y. and Xia, Y. (2007) Synthesis of Silver Nanostructures with Controlled Shapes and Properties. Accounts of Chemical Research, 40, 1067-1076.

[4]   Ulkur, E., Oncul, O., Karagoz, H., Yeniz, E. and Celikoz, B. (2005) Comparison of Silver-Coated Dressing (Acticoat), Chlorhexidine Acetate 0.5% (Bactigrass), and Fusidic Acid 2% (Fucidin) for Topical Antibacterial Effect in Methicillin Resistant Staphylococci-Contaminated, Full-Skin Thickness Rat Burn Wounds. Burns, 31, 874-877.

[5]   Parikh, D.V., Fink, T., Rajasekharan, K., Sachinvala, N.D., Sawhney, A.P.S., Calamari, T.A. and Parikh, A.D. (2005) Antimicrobial Silver/Sodium Carboxymethyl Cotton Dressings for Burn Wounds. Textile Research Journal, 75, 134-138.

[6]   Alt, V., Bechert, T., Steinrücke, P., Wagener, M., Seidel, P., Dingeldein, E., Domann, E. and Schnettler, R. (2004) In Vitro Testing of Antimicrobial Activity of Bone Cement. Antimicrob Agents Chemother, 48, 4084-4088.

[7]   Gosheger, G., Hardes, J., Ahrens, H., Streitburger, A., Buerger, H., Erren, M., Gunsel, A., Kemper, F.H., Winkelmann, W. and Von Eiff, C. (2004) Silver-Coated Megaendoprostheses in a Rabbit Model—An Analysis of the Infection Rate and Toxicological Side Effects. Biomaterials, 25, 5547-5556.

[8]   Rupp, M.E., Fitzgerald, T., Marion, N., Helget, V., Puumala, S., Anderson, J.R. and Fey, P.D. (2004) Effect of Silver-Coated Urinary Catheters: Efficacy, Cost-Effectiveness, and Antimicrobial Resistance. American Journal of Infection Control, 32, 445-450.

[9]   Samuel, U. and Guggenbichler, J.P. (2004) Prevention of Catheter-Rel the Potential of a New Nano Silver Impregnated Catheter. International Journal of Antimicrobial Agents, 23S1, S75-S78.

[10]   Strathmann, M. and Wingender, J. (2004) Use of an Oxonol Dye in Combination with Confocal Laser Scanning Microscopy to Monitor Damage to Staphylococcus aureus Cells during Colonisation of Silver-Coated Vascular Grafts. International Journal of Antimicrobial Agents, 24, 234-240.

[11]   Ohashi, S., Saku, S. and Yamamoto, K. (2004) Antibacterial Activity of Silver Inorganic Agent YDA Filler. Journal of Oral Rehabilitation, 31, 364-367.

[12]   Bosetti, M., Masse, A., Tobin, E. and Cannas, M. (2002) Silver Coated Materials for External Fixation Devices: In Vitro Biocompatibility and Genotoxicity. Biomaterials, 23, 887-892.

[13]   Gauger, A., Mempel, M., Schekatz, A., Schafer, T., Ring, J. and Abeck, D. (2003) Silver Coated Textiles Reduce Staphylococcus aureus Colonization in Patients with Atopic Eczema. Dermatology, 207, 15-21.

[14]   Lee, H.J. and Jeong, S.H. (2005) Bacteriostasis and Skin Innoxiousness of Nanosize Silver Colloids on Textile Fabrics. Textile Research Journal, 75, 551-556.

[15]   Afreen, R.V. and Ranganath, E. (2011) Synthesis of Monodispersed Silver Nanoparticles by Rhizopus Stolonifer and Its Antibacterial Activity against MDR Strains of Pseudomonas Aeruginosa from Burnt Patients. International Journal of Environmental Sciences, 1, 1582-1592.

[16]   Mirzajani, F., Ghassempour, A., Aliahmadi, A. and Esmaeili, M.A. (2011) Antibacterial Effect of Silver Nanoparticles on Staphylococcus aureus. Research in Microbiology, 162, 542-549.

[17]   Pal, S., Tak, Y. and Song, J. (2007) Does the Antibacterial Activity of Silver Nanoparticles Depend on the Shape of the Nanoparticle? A Study of the Gram-Negative Bacterium Escherichia coli. Applied and Environmental Microbiology, 73, 1712-1720.

[18]   Rajawat, S. and Qureshi, M.S. (2012) Comparative Study on Bactericidal Effect of Silver Nanoparticles, Synthesized Using Green Technology, in Combination with Antibiotics on Salmonella typhi. Journal of Biomaterials and Nanobiotechnology, 3, 480-485.

[19]   Boldyrev, V.V. (2002) Thermal Decomposition of Silver Oxalate. Thermochimica Acta, 388, 63-90.

[20]   Navaladian, S., Janet, C.M., Viswanathan, B., Varadarajan, T.K. and Viswanath, R.P. (2007) Afacile Room Temperature Synthesis of Gold Nanowires by Oxalate Reduction Method. The Journal of Physical Chemistry C, 111, 14150-14156.

[21]   Navaladian, S., Viswanathan, B., Viswanath, R.P. and Varadarajan, T.K. (2007) Thermal Decomposition as Route for Silver Nanoparticles. Nanoscale Research Letters, 2, 44-48.

[22]   Mansouri, S.S. and Ghader, S. (2009) Experimental Study on Effect of Different Parameters on Size and Shape of Triangular Silver Nanoparticles Prepared by a Simple and Rapid Method in Aqueous Solution. Arabian Journal of Chemistry, 2, 47-53.

[23]   Kasivelu, G., Sabjan, K., Vijayakumar Ganesh, K. and Ganesan, S. (2008) Silver, Gold and Bimetallic Nanoparticles Production Using Single-Cell Protein (Spirulinaplatensis) Geitler. Journal of Materials Science, 43, 5115-5122.

[24]   Godapati, R.D. (1954) Cong. Luso-Esparn Farm. 3.

[25]   Loo, Y.Y., Chieng, B.W. and Radu, M.N.S. (2012) Synthesis of Silver Nanoparticles by Using Tea Leaf Extract from Camellia Sinensis. International Journal of Nanomedicine, 7, 4263-4267.

[26]   Joshi, M., Bhattacharyya, A. and Wazed Ali, S. (2008) Characterization Techniques for Nanotechnology Applications in Textiles. Indian Journal of Fibre and Textile Research, 33, 304-317.

[27]   (2013) Electronic Supplementary Material (ESI) for Analyst. The Royal Society of Chemistry.

[28]   Van Dong, P., Ha, C.H., Binh, L.T. and Kasbohm, J. (2012) Chemical Synthesis and Antibacterial Activity of Novel-Shaped Silver Nanoparticles. International Nano Letters, 1, 2-9.

[29]   Kvitek, L., Panacek, A., Soukupova, J., Kolar, M., Vecerova, R., Prucek, R., et al. (2008) Effect of Surfactants and Polymers on Stability and Antibacterial Activity of Silver Nanoparticles (NPs). The Journal of Physical Chemistry C, 112, 5824-5825.