AJPS  Vol.5 No.15 , July 2014
Evaluation of Phytoremediation Potential of Catharanthus roseus with Respect to Chromium Contamination
Abstract: A major environmental concern due to dispersal of industrial and urban wastes generated by human activities is the contamination of soil. The release of heavy metals into the terrestrial ecosystem is a major problem. Accumulation of heavy metals in environment and particularly in soil is a serious environmental concern, as the accumulated heavy metal ions can find their way into living organisms via contamination of ground water or food chain. This praxis urgently requires and demands governmental regulations in India. Two samples of sludge were collected from Banthar Industrial Pollution Control Company (BIPCC), UP State Industrial Development Corporation (UPSIDC), Leather Technology Park, Banthar, Unnao, India. In the present study, the phytoremediation potential of Catharanthus roseus, a valued medicinal plant, with respect to chromium has been analyzed. C. roseus was shown to absorb up to about 38% of the amount of Cr present in primary and secondary sludge amended soil through roots and accumulate it to about 22% in leaves. Effect of chromium concentration on the status of antioxidant enzyme peroxidase (POD) and detoxification enzyme glutathione-S-transferase (GST) from C. roseus leaves was also observed and determined. Increased expressions of POD and GST were observed on native PAGE under stress conditions as compared to control. C. roseus can well tolerate low amounts of chromium (and accumulate it to about 22% in leaves) and can, thus, prove useful in the reclamation and remediation of chromium contaminated soil and land.
Cite this paper: Ahmad, R. and Misra, N. (2014) Evaluation of Phytoremediation Potential of Catharanthus roseus with Respect to Chromium Contamination. American Journal of Plant Sciences, 5, 2378-2388. doi: 10.4236/ajps.2014.515251.

[1]   Meagher, R.B. (2000) Phytoremediation of Toxic Elemental and Organic Pollutants. Current Opinion in Plant Biology, 3, 153-162.

[2]   Ghosh, M. and Singh, S.P. (2005) A Review on Phytoremediation of Heavy Metals and Utilization of Its Byproducts. Applied Ecology and Environmental Research, 3, 1-18.

[3]   Antolin, M.C., Muro, I. and Sanchez-Diaz, M. (2010) Sewage Sludge Application Can Induce Changes in Antioxidant Status of Nodulated Alfalfa Plants. Ecotoxicology and Environmental Safety, 73, 436-442.

[4]   Mishra, M. and Dey, S.K. (2012) Paper Sludge Induced Physiological Changes in the Antioxidative Response System of Solanum melongena L. Journal of Pharmacy and Biological Sciences, 4, 40-42.

[5]   Park, D., Yun, Y.S. and Park, J.M. (2005) Use of Dead Fungal Biomass for the Detoxification of Hexavalent Chromium: Screening and Kinetics. Process Biochemistry, 40, 2559-2565.

[6]   Wang, X.J., Chen, L., Xia, S.Q., Zhao, J.F., Chovelon, J.-M. and Renault, N.J. (2006) Biosorption of Cu (II) and Pb (II) from Aqueous Solutions by Dried Activated Sludge. Minerals Engineering, 19, 968-971.

[7]   Smirnoff, N. (1993) The Role of Active Oxygen in the Response of Plants to Water Deficit and Desiccation. New Phytologist, 125, 27-58.

[8]   Foyer, C.H., Descourvieres, P. and Kunert, K.J. (1994) Protection against Oxygen Radicals: An Important Defence Mechanism Studied in Transgenic Plants. Plant Cell and Environment, 17, 507-523.

[9]   Foyer, C.H., Lopez-Delgado, H., Dat, J.F. and Scott, I.M. (1997) Hydrogen Peroxide and Glutathione-Associated Mechanisms of Acclimatory Stress Tolerance and Signaling. Physiologia Plantarum, 100, 241-254.

[10]   Gressel, J. and Galun, E. (1994) Causes of Photooxidative Stress and Amelioration of Defense Systems in Plant. In: Foyer, C.H. and Mullineaux, P.M., Eds., Genetic Controls of Photooxidant Tolerance, CRC Press, Boca Raton, 237-274.

[11]   Marrs, K.A. (1996) The Functions and Regulation of Glutathione-S-Transferases in Plants. Annual Review of Plant Physiology and Molecular Biology, 47, 127-158.

[12]   Odjegba, V. and Fasidi, I. (2007) Phytoremediation of Heavy Metals by Eichhornia crassipes. The Environmentalist, 27, 349-355.

[13]   Misra, N. and Gupta, A.K. (2006) Effect of Salinity and Different Nitrogen Sources on the Activity of Antioxidant Enzymes and Indole Alkaloid Content in Catharanthus roseus Seedlings. Journal of Plant Physiology, 163, 11-18.

[14]   Srivastava, N.K. and Srivastava, A.K. (2010) Influence of Some Heavy Metals on Growth, Alkaloid Content and Composition in Catharanthus roseus L. Indian Journal of Pharmaceutical Sciences, 72, 775-778.

[15]   Pandey, S., Gupta, K. and Mukherjee, A.K. (2007) Impact of Cadmium and Lead on Catharanthus roseus—A Phytoremediation Study. Journal of Environmental Biology, 28, 655-662.

[16]   Zheng, Z. and Wu, M. (2004) Cadmium Treatment Enhances the Production of Alkaloid Secondary Metabolites of Catharanthus roseus. Plant Science, 166, 507-514.

[17]   Bartlett, R.J. (1991) Chromium Cycling in Soils: Links, Gaps, and Methods. Environmental Health Perspectives, 92, 17-24.

[18]   Bini, C., Gentili, L., Maleci-Bini, L. and Vaselli, O. (1995) Trace Elements in Plants and Soils of Urban Parks. Annexed to Contaminated Soil Prost, INRA, Paris.

[19]   Ahmad, R., Srivastava, A.K. and Walter, R.D. (2008) Purification and Biochemical Characterization of Cytosolic Glutathione-S Transferase from Filarial Worms Setaria cervi. Comparative Biochemistry and Physiology Part B, 151, 237-245.

[20]   Putter, J. (1974) Peroxidase. In: Bergmeyer, H.U., Ed., Methods of Enzymatic Analysis, Verlag Chemie, Weinhan, 685-690.

[21]   Habig, W.H., Pabst, M.J. and Jakoby, W.B. (1974) Glutathione-S-Transferases. The First Enzymatic Step in Mercapturic Acid Formation. Journal of Biological Chemistry, 246, 7130-7139.

[22]   Laemmli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685.

[23]   Ricci, G., Bello, M.L., Caccuri, A.M., Galiazzo, F. and Federici, G. (1984) Detection of Glutathione-S-Transferase Activity on Polyacrylamide Gels. Analytical Biochemistry, 143, 226-230.

[24]   Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein Measurement with Folin Phenol Reagent. Journal of Biological Chemistry, 193, 265-275.

[25]   Endo, T., Goodbody, A. and Misawa, M. (1987) Alkaloid Production in Root and Shoot Cultures of Catharanthus roseus. Plant Medica, 53, 479-482.

[26]   Singh, A., Eapen, S. and Fulekar, M.H. (2009) Phytoremediation Technology for Remediation of Radiostrontium (90SR) and Radiocaesium (137CS) by Catharanthus roseus (L.) G. Don in Aquatic Environment. Environmental Engineering and Management Journal, 8, 527-532.

[27]   Maksymiec, W. (2007) Signalling Responses in Plants to Heavy Metal Stress. Acta Physiologiae Plantarum, 29, 177-187.

[28]   Singh, O., Khanam, Z., Misra, N. and Srivastava, M.K. (2011) Chamomile (Matricaria chamomilla L.): An Overview. Pharmacognosy Reviews, 5, 82-95.

[29]   Reeves, R.D. (2003) Tropical Hyperaccumulators of Metals and Their Potential for Phytoextraction. Plant and Soil, 249, 57-65.

[30]   Rulkens, W.H., Tichy, R. and Grotenhuis, J.T.C. (1998) Remediation of Polluted Soil and Sediment: Perspectives and Failures. Water Science and Technology, 37, 27-35.

[31]   Salt, D.E., Smith, R.D. and Raskin, I. (1998) Phytoremediation. Annual Reviews of Plant Physiology and Plant Molecular Biology, 49, 643-668.

[32]   Dushenkov, D. (2003) Trends in Phytoremediation of Radionuclides. Plant and Soil, 249, 167-175.

[33]   Kochian, L. (1996) Mechanisms of Heavy Metal Transport across Cell Membranes. Paper Presented at International Phytoremediation Conference, Southborough.

[34]   Brown, S.L., Chaney, R.L., Angle, J.S. and Baker, A.J.M. (1995) Zinc and Cadmium Uptake by Hyperaccumulator Thlaspi caerulescens Grown in Nutrient Solution. Soil Science Society of America Journal, 59, 125-133.

[35]   Gerard, E., Echevarria, G., Sterckeman, T. and Morel, J.L.P. (2000) Availability of Cd to Three Plant Species Varying in Accumulation Pattern. Journal of Environmental Quality, 29, 1117-1123.

[36]   Henry, J.R. (2000) An Overview of Phytoremediation of Lead and Mercury. NNEMS Report, Washington DC, 3-9.