JBNB  Vol.5 No.3 , July 2014
Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma
ABSTRACT

In this work, we describe an approach of detecting biomarkers by Surface Plasmon Resonance imaging (SPRi) technique in real samples. Two C-Reactive Protein (CRP)-antibody immobilization methods were used: The first method was based on direct physisorption of CRP-antibody onto gold surface; the second one was based on oriented CRP-antibody with protein G intermediate layer. The two developed immunosensors were tested against CRP antigen in phosphate buffer saline solution with the SPRi technique. The response of the developed immunosensors was reproducible and stable. The detection limit of 10 pg&#183mL&#451 and 50 pg&#183mL&#451 CRP-antigen was observed with and without protein G respectively with this technique. Moreover, the developed SPRi immunosensor was used for CRP-antigen detection in human plasma. A detection limit of 5 ng&#183mL&#451 and 10 ng&#183mL&#451 was obtained with and without protein G respectively. These obtained results were compared to those obtained with QCM (Quartz Crystal Microbalance) and Enzyme-Linked Immunosorbent Assay (ELISA) techniques.


Cite this paper
Chammem, H. , Hafaid, I. , Meilhac, O. , Menaa, F. , Mora, L. and Abdelghani, A. (2014) Surface Plasmon Resonance for C-Reactive Protein Detection in Human Plasma. Journal of Biomaterials and Nanobiotechnology, 5, 153-158. doi: 10.4236/jbnb.2014.53018.
References
[1]   Ramadan, M., Shrive, A., Holden, D., Miles, D., Volanakis, J., Delucas, L. and Greenhough, T. (2002) Development of a Disposable Potentiometric Antibody Immobilized ZnO Nanotubes Based Sensor for the Detection of C-Reactive Protein. Acta Crystallogr, 58, 992-997.
http://dx.doi.org/10.1016/j.snb.2012.03.083

[2]   Sklavou, R., Karavanaki, K., Critselis, E., Kossiva, L., Giannaki, M., Tsolia, M., Papadakis, V., Papargyri, S., Vlachou, A., Karantonis, F., Gourgiotis, D. and Polychronopoulou, S. (2012) Variation of Serum C-Reactive Protein (CRP) over Time in Pediatric Cancer Patients with Febrile Illness and Its Relevance to Identified Pathogen. Clinical Biochemistry, 45, 1178-1182.
http://dx.doi.org/10.1016/j.clinbiochem.2012.07.088

[3]   Parra, M.D., Tuomola, M., Cabezas-Herrera, J. and Ceron, J.J. (2005) Use of a Time-Resolved Immunofluorometric Assay for Determination of Canine C-Reactive Protein Concentrations in Whole Blood. American Journal of Veterinary Research, 66, 62-66.

[4]   Hafaid, I., Chammem, H., Abdelghani, A., Ait, E., Feldman, L., Meilhac, O. and Mora, L. (2013) Supported Protein G on Gold Electrode: Characterization and Immunosensor Application. Talanta, 116, 84-90.
http://dx.doi.org/10.1016/j.talanta.2013.04.059

[5]   Woodbury, R.G., Wendin, C., Clendenning, J., Melendez, J., Elkind, J., Bartholomew, D., Brown, S. and Furlong, C.E. (1998) Construction of Biosensors Using a Gold-Binding Polypeptide and a Miniature Integrated Surface Plasmon Resonance Sensor. Biosensors & Bioelectronics, 13, 1117-1126.
http://dx.doi.org/10.1016/S0956-5663(98)00060-8

[6]   Vikinge, T.P., Askendal, A., Liedberg, B., Lindahl, T. and Tengvall, P. (1998) Immobilized Chicken Antibodies Improve the Detection of Serum Antigens with Surface Plasmon Resonance (SPR) (SPR). Biosensors & Bioelectronics, 13, 1257-1262.
http://dx.doi.org/10.1016/S0956-5663(98)00085-2

[7]   Campagnolo, C., Meyers, K.J., Ryan, T., Atkinson, R.C., Chen, Y.T., Scanlan, M.J., Ritter, G., Old, L.J. and Batta, C.A. (2004) Real-Time, Label-Free Monitoring of Tumor Antigen and Serum Antibody Interactions. Journal of Biochemical and Biophysical Methods, 61, 283-298.
http://dx.doi.org/10.1016/j.jbbm.2004.05.006

[8]   Gan, N., Xiong, P., Wang, J., Li, T., Hu, F., Cao, Y. and Zheng, L. (2013) A Novel Signal-Amplified Immunoassay for the Detection of C-Reactive Protein Using HRP-Doped Magnetic Nanoparticles as Labels with the Electrochemical Quartz Crystal Microbalance as a Detector. Journal of Analytical Methods in Chemistry, 2013, 8 p.
http://dx.doi.org/10.1155/2013/482316

[9]   Baccar, H., Mejri, M.B., Hafaid, I., Ktari, T., Aouini, M. and Abdelghani, A. (2010) Surface Plasmon Resonance Immunosensor for Bacteria Detection. Talanta, 82, 810-814.
http://dx.doi.org/10.1016/j.talanta.2010.05.060

[10]   Löfås, S. (2007) Biacore-Creating the Business of Label-Free Protein-Interaction Analysis. In: Marks, R.S., Eds., Handbook of Biosensors and Biochips, Vol. 1, John Wiley & Sons, Chichester, 1261-1272.

[11]   Meyer, M.H.F., Hartmann, M. and Keusgen, M. (2006) SPR-Based Immunosensor for the CRP Detection—A New Method to Detect a Well Known Protein. Biosensors and Bioelectronics, 21, 1987-1990.
http://dx.doi.org/10.1016/j.bios.2005.09.010

[12]   Vermeeren, V., Grieten, L., Vanden Bon, N., Bijnens, N., Wenmackers, S., Janssens, S.D., Haenen, K., Wagner, P. and Michiels, L. (2011) Impedimetric, Diamond-Based Immmunosensor for the Detection of C-Reactive Protein. Sensors and Actuators, 157, 130-138.
http://dx.doi.org/10.1016/j.snb.2011.03.037

[13]   Turková, J. (1999) Oriented Immobilization of Biologically Active Proteins as a Tool for Revealing Protein Interactions and Function. Journal of Chromatography B: Biomedical Sciences and Applications, 722, 11-31.
http://dx.doi.org/10.1016/S0378-4347(98)00434-4

 
 
Top