ABB  Vol.5 No.7 , June 2014
Isolation and Characterization of Pepsin-Solubilized Collagen from the Skin of Black Carp (Mylopharyngdon piceus)
ABSTRACT

To make more effective use of underutilized resources, pepsin-solubilized collagen (PSC) was successfully extracted from the skin of black carp (Mylopharyngdon piceus) with a yield of 45.7% based on dry weight. The PSC comprising two identical α1-chains and one α2-chain with no disulfide bond was characterized as type I, and it contained 195 imino acid residues vs. 1000 amino acid residues. The collagen showed an absorption edge around 218 nm, which was lower than the maximum absorption wavelength of other PSC. The denaturation temperature of PSC was 25.6°C, which was lower than that of porcine collagen by approximately 11°C. The isoelectric point (pI) was estimated to be 8.23, and the collagen was soluble at an acidic pH as well as below 40 g/L NaCl. It is thought that the high yield and stability of PSC from the skin of black carp warrant its application as a new source of collagen for industrial purposes.


Cite this paper
Wu, G. , Wang, X. , Lin, L. , Chen, S. and Wu, Q. (2014) Isolation and Characterization of Pepsin-Solubilized Collagen from the Skin of Black Carp (Mylopharyngdon piceus). Advances in Bioscience and Biotechnology, 5, 642-650. doi: 10.4236/abb.2014.57076.
References
[1]   Nalinanon, S., Benjakul, S., Kishimura, H. and Osako K. (2011) TypeⅠCollagen from the Skin of Ornate Threadfin Bream (Nemipterus hexodon): Characteristics and Effect of Pepsin Hydrolysis. Food Chemistry, 125, 500-507.
http://dx.doi.org/10.1016/j.foodchem.2010.09.040

[2]   Xiong, X., Ghosh, R., Hiller, E., Drepper, F., Knapp, B., Brunner H., and Rupp, S. (2009) A New Procedure for Rapid, High Yield Purification of Type I Collagen for Tissue Engineering. Process Biochemistry, 44, 1200-1212.
http://dx.doi.org/10.1016/j.procbio.2009.06.010

[3]   Pati, F., Adhikari, B. and Dhara, S. (2010) Isolation and Characterization of Fish Scale Collagen of Higher Thermal Stability. Bioresource Techonlogy, 101, 3737-3742.
http://dx.doi.org/10.1016/j.biortech.2009.12.133

[4]   Zeng, S.K., Yin, J.J., Yang, S.Q., Zhang, C.H., Yang, P. and Wu, W.L. (2012) Structure and Characteristics of Acid and Pepsin-Solubilized Collagens from the Skin of Cobia (Rachycentron canadum). Food Chemistry, 135, 1975-1984.
http://dx.doi.org/10.1016/j.foodchem.2012.06.086

[5]   Zhang, Y., Liu, W., Li, G., Shi, B., Yu, Q. and Wu, X. (2007) Isolation and Partial Characterization of Pepsin-Soluble Collagen from the Skin of Grass Carp (Ctenopharyngodon idella). Food Chemistry, 103, 906-912.
http://dx.doi.org/10.1016/j.foodchem.2006.09.053

[6]   Laemmli, U.K. (1970) Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4. Nature, 227, 680-685.
http://dx.doi.org/10.1038/227680a0

[7]   Huang, Y., Shiau, C., Chen, H. and Huang, B. (2011) Isolation and Characterization of Acid and Pepsin-Solubilized Collagens from the Skin of Balloon Fish (Diodon holocanthus). Food Hydrocolloids, 25, 1507-1513.
http://dx.doi.org/10.1016/j.foodhyd.2011.02.011

[8]   Lowry, O.H., Rosebrough, N.J., Farr, A.L. and Randall, R.J. (1951) Protein Measurement with Folin Phenol Reagent. Journal of Biological Chemistry, 193, 256-275.

[9]   Nagai, T., Araki, Y. and Suzuki, N. (2002) Collagen of the Skin of Ocellate Puffer Fish (Takifugu rubripes). Food Chemistry, 78, 173-177.
http://dx.doi.org/10.1016/S0308-8146(01)00396-X

[10]   Jongjareonrak, A., Benjakul, S., Vesessangguan, W., Nagai, T. and Tanaka, M. (2005) Isolation and Characterization of Acid and Pepsin-Solubilized Collagen from the Skin of Brownstripe Red Snapper (Lutjanus vitta). Food Chemistry, 93, 475-484.
http://dx.doi.org/10.1016/j.foodchem.2004.10.026

[11]   Ogawa, M., Moody, M.W., Portier, R.J., Bell, J., Schexnayder, M.A., and Losso, J.N. (2003) Biochemical Properties of Black Drum and Sheephead Seabream Skin Collagen. Journal of Agricultural and Food Chemistry, 51, 8088-8092.
http://dx.doi.org/10.1021/jf034350r

[12]   Kimura, S. (1992) Wide Distribution of the TypeⅠCollagen α3 Chain in Bony Fish. Comparative Biochemistry and Physiology, 102B, 255-260.

[13]   Sato, K., Ebihara, T., Adachi, E., Kawashima, S., Hattori, S. and Irie, S. (2000) Possible Involvement of Aminotelopeptide in Self-Assembly and Thermal Stability of Collagen I as Revealed by Its Removal with Protease. Journal of Biological Chemistry, 275, 25870-25875.
http://dx.doi.org/10.1074/jbc.M003700200

[14]   Ahmad, M. and Benjakul, S. (2010) Extraction and Characterization of Pepsin-Solubilized Collagen from the Skin of Unicorn Leatherjacket (Aluterus monocerous). Food Chemistry, 120, 817-824.
http://dx.doi.org/10.1016/j.foodchem.2009.11.019

[15]   Zhang, M., Liu, W. and Li, G. (2009) Isolation and Characterization of Collagens from the Skin of Largefin Longbarbel Catfish (Mystus macropterus). Food Chemistry, 115, 826-831. http://dx.doi.org/10.1016/j.foodchem.2009.01.006

[16]   Nagai, T., Suzuki, N. and Nagashima, T. (2008) Collagen from Common Minke Whale (Balaenoptera acurorostrata) Unesu. Food Chemistry, 111, 296-301.
http://dx.doi.org/10.1016/j.foodchem.2008.03.087

[17]   Matmaroh, K., Benjakul, S., Prodpran, T., Encarnacion, A.B. and Kishimura, H. (2011) Characteristics of Acid Soluble Collagen and Pepsin Soluble Collagen from Scale of Spotted Golden Goatfish (Parupeneus heptacanthus). Food Chemistry, 129, 1179-1186.
http://dx.doi.org/10.1016/j.foodchem.2011.05.099

[18]   Nagai, T. and Suzuki, N. (2000) Isolation of Collagen from Fish Waste Material—Skin, Bone and Fins. Food Chemistry, 68, 277-281.
http://dx.doi.org/10.1016/S0308-8146(99)00188-0

[19]   Foegeding, E.A., Lanier, T.C. and Hultin, H.O. (1996) Characteristics of edible Muscle Tissues. In: Fennema, O.R., Ed., Food Chemistry, Marcel Dekker Inc., New York, 902-906.

[20]   Vojdani, F. (1996) Solubility. In: Hall, G.M., Ed., Methods of Testing Protein Functionality. St. Edmundsbury Press, London, 11-60.
http://dx.doi.org/10.1007/978-1-4613-1219-2_2

[21]   Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Nagai, T. and Tanaka, M. (2005) Characterization of Acid -Soluble Collagen from Skin and Bone of Bigeye Snapper (Priacanthus tayenus). Food Chemistry, 89, 363-372.
http://dx.doi.org/10.1016/j.foodchem.2004.02.042

[22]   Liu, D.S., Liang, L., Regenstein, J.M. and Zhou, P. (2012) Extraction and Characterization of Pepsin-Solubilised Collagen from Fines, Scales, Skin, Bones and Swim Bladders of Bighead Carp (Hypophthalmichthys nobilis). Food Chemistry, 133, 1441-1448.
http://dx.doi.org/10.1016/j.foodchem.2012.02.032

 
 
Top