Use of a single seed is very useful for genetic studies on Vitis vinifera. However, molecular markers require a fair amount of high purity DNA. Grapevine contains high concentrations of polysaccharides, polyphenols, tannins and other secondary metabolites. These compounds may hamper the DNA isolation processes and subsequent analysis. In this study we have compared two DNA isolation methods: the NucleoSpin Plant II method and a modified protocol from Doyle and Doyle. The average value of 260/280 nm absorption ratio, which is used to assess the purity of DNA and RNA was 1.8 (accepted as “pure” DNA) and 0.9 (presence of protein or other contaminants) for the first and second method, respectively. Using the NucleoSpin protocol, from a single seed (20 - 35 mg) we obtained an average yield of extracted DNA of 24.8 ± 5.2 to 38.4 ± 11.5 ng·mg-1 dry weight. Among the two protocols examined, the NucleoSpin method was more efficient and gave better quality of DNA values compared to those from the modified Doyle and Doyle procedures.
 Arumuganathan, K. and Earle, E.D. (1991) Nuclear DNA Content of Some Important Plant Species. Plant Molecular Biology Reporter, 9, 208-218.
 Kikkert, J.R., Striem, M.J., Vidal, J.R., Wallace, P.G., Barnard, J. and Reisch, B.I. (2005) Long-Term Study of Somatic Embryogenesis from Anthers and Ovaries of 12 Grapevine (Vitis sp.) Genotypes. In Vitro Cellular & Developmental Biology-Plant, 41, 232-239.
 Akkurt, M., Welter, L., Maul, E., Topfer, L. and Zyprian, E. (2007) Development of SCAR Markers Linked to Powdery Mildew (Uncinulanecator) Resistance in Grapevine (Vitis vinifera L. and Vitis sp.). Molecular Breeding, 19, 103-111.
 Troggio, M., et al. (2007) A Dense Single-Nucleotide Polymorphism-Based Genetic Linkage Map of Grapevine (Vitis vinifera L.) Anchoring Pinot Noir Bacterial Artificial Chromosome Contigs. Genetics, 176, 2637-2650.
 Wang, Q., Li, P., Hanania, U., Sahar, N., Mawassi, M., Gafny, R., Sela, I., Tanne, E. and Perl, A. (2005) Improvement of Agrobacterium-Mediated Transformation Efficiency and Transgenic Plant Regeneration of Vitis vinifera L. by Optimizing Selection Regimes and Utilizing Cryopreserved Cell Suspensions. Plant Science, 168, 565-571.
 Adam-Blondon, A.F., Roux, C., Claux, D., But-terlin, G., Merdinoglu, D. and This, P. (2004) Mapping 245 SSR Markers on the Vitis vinifera Genome: A Tool for Grape Genetics. Theoretical and Applied Genetics, 109, 1017-1027.
 DiGaspero, G., Cipriani, G., Adam-Blondon, A.F. and Testolin, R. (2007) Linkage Maps of Grapevine Displaying the Chromosomal Locations of 420 Microsatellite Markers and 82 Markers for R-Gene Candidates. Theoretical and Applied Genetics, 114, 1249-1263.
 Lodhi, M.A., Ye, G.N., Weeden, N.F. and Reisch, B.I. (1994) A Simple and Efficient Method for DNA Extraction from Grapevine Cultivars and Vitis Species. Plant Molecular Biology Reporter, 12, 6-13.
 Hanania, U., Velcheva, M., Sahar, N. and Perl, A. (2004) An Improved Method for Isolating High-Quality DNA from Vitis vinifera Nuclei. Plant Molecular Biology Reporter, 22, 173-177.
 Boso, S., Alonso-Villaverde, V., Martinez, M.C. and Kassemeyer, H.H. (2012) Quantification of Stilbenes in Vitis Genotypes with Different Levels of Resistance to Plasmopara viticola Infection. American Journal of Enology and Viticulture, 63, 419-423.
 Iandolino, A.B., Goes DaSilva, F., Lim, H., Cho, H., Williams, L.E. and Cook, D.R. (2004) High-Quality RNA, cDNA, and Derived EST Libraries from Grapevine (Vitis vinifera L.). Plant Molecular Biology Reporter, 22, 269-278.
 Murray, M.G. and Thompson, W.F. (1980) Rapid Isolation of High Molecular Weight Plant DNA. Nucleic Acids Research, 8, 4321-4325.
 Katterman, F.R.H. and Shattuck, V.L. (1983) An Effective Method of DNA Isolation from the Mature Leaves of Gossypium Species that Contain Large Amounts of Phenolic Terpenoids and Tannins. Preparative Biochemistry, 13, 347-359.
 Loomis, W.D. (1974) Overcoming Problems of Phenolic and Quinines in the Isolation of Plant Enzymes and Organelles. Methods in Enzymology, 31, 528-544.
 Porebski, S., Bailey, L.G. and Baum, B.R. (1997) Modification of a CTAB DNA Extraction Protocol for Plants Containing High Polysaccharide and Polyphenol Components. Plant Molecular Biology Reporter, 15, 8-15.
 Aljanabi, S.M., Forget, L. and Dookun, A. (1999) An Improved and Rapid Protocol for the Isolation of Polysaccharide and Polyphenol Free Sugarcane DNA. Plant Molecular Biology Reporter, 17, 281.
 Akkurt, M. (2012) Comparison between Modified DNA Ex-traction Protocols and Commercial Isolation Kits in Grapevine (Vitis vinifera L.). Genetics and Molecular Research, 11, 2342-2351.
 Marsal, G., Baiges, I., Canals, F., Zamora, J.M. and Fort, F. (2011) A Fast, Efficient Method for Extracting DNA from Leaves, Stems, and Seeds of Vitis vinifera L. American Journal of Enology and Viticulture, 62, 376-381.
 Manen, J.F., Bouby, L., Dalnoki, O., Marinval, P., Turgay, M. and Schlumbaum, A. (2003) Microsatellites from Archaeological Vitis vinifera Seeds Allow a Tentative Assignment of the Geographical Origin of Ancient Cultivars. Journal of Archaeological Science, 30, 721-729.
 Pandey, R., Adams, R.P. and Flournoy, L.E. (1996) Inhibition of Random Amplified Polymorphic DNAs (RAPDs) by Plant Polysaccharides. Plant Molecular Biology Reporter, 14, 17-22.
 Khanuja, S.P.S., Shasany, A.K., Darokar, M.P. and Kumar, S. (1999) Rapid Isolation of DNA from Dry and Fresh Samples of Plants Producing Large Amounts of Secondary Metabolites and Essential Oils. Plant Molecular Biology Reporter, 17, 74.
 Li, J.T., Yang, J., Chen, D.C., Zhang, X.L. and Tang, Z.S. (2007) An Optimized Mini-Preparation Method to Obtain High-Quality Genomic DNA from Mature Leaves of Sun-flower. Genetics and Molecular Research, 6, 1064-1071.
 Collins, G.G. and Symons, R.H. (1992) Extraction of Nuclear DNA from Grape Vine Leaves by a Modified Procedure. Plant Molecular Biology Reporter, 10, 233-235.