Effect of Osmotic Stress on the Initial Development of Bean Seedlings

Pablo Diego Silva  Cabral; Leonardo N. S. dos  Santos; Henrique Duarte  Vieira; Tais Cristina Bastos  Soares; Cintia Aparecida  Bremenkamp; Weverton Pereira  Rodrigues

doi:10.4236/ajps.2014.513211

AJPS Vol.5 No.13 , June 2014

Effect of Osmotic Stress on the Initial Development of Bean Seedlings

Pablo Diego Silva Cabral^*, Leonardo N. S. dos Santos, Henrique Duarte Vieira, Tais Cristina Bastos Soares, Cintia Aparecida Bremenkamp, Weverton Pereira Rodrigues

Abstract: One of the methods to determine the tolerance of plants to water stress is the observation of germination of seeds under stress induced in laboratory conditions. Thus, the aim was to evaluate the response of four common bean cultivars to water deficit simulated under laboratory conditions. Two common landrace genotypes acquired from farmers (FORT 07 and FORT 08) and two commercial cultivars, IAPAR 81 and Serrano were evaluated. The different levels of water deficit were induced with mannitol solution. The experimental design was completely randomized and a factorial scheme 5 × 4 (osmotic potential x genotypes), with four replications. The characteristics: primary root protrusion, seed vigor, normal and abnormal seedling, seedling and root length, fresh and dry weight of seedling and root were evaluated. By reducing the osmotic potential seedling vigor bean was more affected than the primary root protrusion. Regardless of cultivar, there was a significant and progressive reduction in the normal seedlings percentage from potential -0.3 MPa. The IAPAR 81 and FORT 07 cultivars showed higher tolerance to water deficit compared to other cultivars.

Keywords: Phaseolus vulgaris L., Mannitol, Water Stress

Cite this paper: Cabral, P. , Santos, L. , Vieira, H. , Soares, T. , Bremenkamp, C. and Rodrigues, W. (2014) Effect of Osmotic Stress on the Initial Development of Bean Seedlings. American Journal of Plant Sciences, 5, 1973-1982. doi: 10.4236/ajps.2014.513211.

References

[1] Amorim, J.R.A., Fernandes, P.D., Gheyi, H.R. and Azevedo, N.C. (2002) Effect of Irrigation Water Salinity and Its Mode of Application on Garlic Growth and Production. Pesquisa Agropecuária Brasileira, 37, 167-176.

[2] Ferreira, G.S., Torres, S.B. and Costa, A.R.F.C. (2007) Germination and Initial Development Stage of Melon Seedlings at Different Levels of Salinity of Irrigation Water. Caatinga, 20, 181-185.

[3] Munns, R. (2002) Comparative Physiology of Salt and Water Stress. Plant, Cell and Environment, 28, 239-250.
http://dx.doi.org/10.1046/j.0016-8025.2001.00808.x

[4] Silva, L.S., Silva, R.B., Valadares, R.N., Matos, V.P. and Lima, C.R. (2012) Behavior of Rice Seed under Different Osmotic Potentials. Revista Tropica: Ciências Agrárias e Biológicas, 6, 37-44.

[5] Andréo-Souza, Y., Pereira, A.L., Silva, F.F.S., Riebeiro-Reis, R.C., Evangelista, M.R.V., Castro, R.D. and Dantas, B.F. (2010) Effect of Salinity on Physic Nut (Jatropha curcas L.) Seed Germination and Seedling Initial Growth. Revista Brasileira de Sementes, 32, 83-92.

[6] Lacerda, C.F., Cambraia, J., Oliva, M.A. and Ruiz, H.A. (2003) Osmotic Adjustment in Roots and Leaves of Two Sorghum Genotypes under NaCl Stress. Brazilian Journal of Plant Physiology, 15, 113-118.
http://dx.doi.org/10.1590/S1677-04202003000200007

[7] Oliveira, F.R.A., Oliveira, F.A., Guimarães, I.P., Medeiros, J.F., Oliveira, M.K.T., Freitas, A.V.L. and Medeiros, M.A. (2009) Emergency of Seedlings of Moringa Oleifera Lam Irrigated with Water of Different Levels of Salinity. Bioscience Journal, 25, 66-74.

[8] ávila, M.R., Braccini, A.L., Scapim, C.A., Fagliari, J.R. and Santos, J.L. (2007) Influence of Manitol-Induced Water Stress on Canola Seed Germination and Seedling Growth. Revista Brasileira de Sementes, 29, 98-106.
http://dx.doi.org/10.1590/S0101-31222007000100014

[9] Coelho, D.L.M., Agostini, E.A.T., Guaberto, L.M., Ma-chado Neto, N.B. and Custódio, C.C. (2010) Differential Protein Expression during Germination as a Result of a Water Deficit Associated with Variable Osmotic Pressure in Snap-Beans. Acta Scientiarum Agronomy, 32, 491-499.

[10] Custódio, C.C., Salomão, G.R. and Machado Neto, N.B. (2009) Water Deficiency during Bean Seed Germination Induced by Different Osmotic Solutions. Revista Ciência Agronômica, 40, 617-623.

[11] Forti, V.A., Cicero, S.C. and Pinto, T.L.F. (2009) Influ-ence of Water Availability of the Substratum and Moisture Content of the Bean Seeds on the Germination. Revista Brasileira de Sementes, 31, 63-70.
http://dx.doi.org/10.1590/S0101-31222009000200007

[12] Neto, N.B.M., Custódio, C.C., Costa, P.R. and Doná, F.L. (2006) Water Stress Induced by Different Osmotic Agentes on Germination and Vigor of Bean Seeds. Revista Brasileira de Sementes, 28, 142-148.
http://dx.doi.org/10.1590/S0101-31222006000100020

[13] Moterle, L.M., Lopes, P.C., Braccini, A.L. and Scapim, C.A. (2006) Germination of Seeds and Seedling Growth of Popcorn Cultivars under Water and Salinity Stress. Revista Brasileira de Sementes, 28, 169-176.
http://dx.doi.org/10.1590/S0101-31222006000300024

[14] Costa, P.R. (2006) Water Defficit: Osmotic Agents and Seed Size Study. Master’s Thesis, Universidade do Oeste Paulista, São Paulo.

[15] Moraes, G.A.F., Menezes, N.L. and Pasqualli, L.L. (2005) Bean Seed Performance under Different Osmotic Potentials. Ciência Rural, 35, 776-780.
http://dx.doi.org/10.1590/S0103-84782005000400004

[16] Larcher, W. (2000) Plant Ecology. Rima, São Carlos.

[17] Cabral, P.D.S., Soares, T.C.B., Gonçalves, L.S.A., Amaral Júnior, A.T., Lima, A.B.P., Rodrigues, R. and Matta, F.P. (2010) Quantification of the Diversity among Common Bean Accessions Using Ward-MLM Strategy. Pesquisa Agropecuária Brasileira, 45, 1124-1132.

[18] Cabral, P.D.S., Soares, T.C.B., Lima, A.B.P., Miranda, F.D., Souza, F.B. and Gonçalves, L.S.A. (2011) Genetic Diversity in Local and Commercial Dry Bean (Phaseolus vulgaris) Accessions Based on Microsatellite Markers. Genetics and Molecular Research, 10, 140-149.
http://dx.doi.org/10.4238/vol10-1gmr993

[19] Braga, L.F., Sousa, M.P., Braga, J.F. and Sá, M.A.E. (1999) Relationship among Physiological Quality and Water Availability of Bean Seeds on the Germination Process. Revista Brasileira de Sementes, 21, 95-102.

[20] Brasil, Ministério da Agricultura Agropecuária e Abastecimento (2009) Secretaria de Defesa Agropecuária. Regras para Análise de Sementes, Brasília.

[21] Cruz, C.D. (2013) GENES—A Software Package for Analysis in Experimental Statistics and Quantitative Genetics. Acta Scientiarum. Agronomy, 35, 271-276.

[22] Coelho, C.M.M., Mota, M.R., Souza, C.A. and Miquelluti, D.J. (2010) Physiological Potential of Common Landrace Bean Seeds (Phaseolus vulgaris L.). Revista Brasileira de Sementes, 2, 97-105.

[23] Pereira, L.A.G., Costa, N., Almeida, A.M.R., Silva, C.M. and Sartori, J.F. (1981) Interaction Effect of Chemical Treatment of Soybean Seeds and Vigor Levels. Fitopatologia Brasileira, 6, 159-1631.

[24] Neto, N.B.M., Saturnino, S.M., Bomfim, D.C. and Custódio, C.C. (2004) Water Stress Induced by Mannitol and Sodium Chloride in Soybean Cultivars. Brazilian Archives of Biology and Technology, 47, 521-529.
http://dx.doi.org/10.1590/S1516-89132004000400004