Cold stress, which causes dehydration damage to plants, is one of the most common abiotic stresses that limit plant distributions and affect crop growth and development. To improve their cold tolerance, plants often upregulate the expression of some cold-related genes. In this study, a cold-regulated (COR) gene was isolated from Vitis amurensis and designated as VaCOR. RT-PCR analysis demonstrated that VaCOR was expressed at high levels in the roots, stems, leaves, and petioles under low temperature, but it was not detected under normal temperatures. Further analysis revealed that salinity and the application of exogenous abscisic acid and salicylic acid significantly induced VaCOR transcription, with apparent differences in its expression in different organs. The data also showed that COR gene expression was higher in cold-resistant wild V. amurensis than in cold-sensitive Vitis vinifera “Manicure Finger” under low temperature. These results suggest that the VaCOR gene in V. amurensis grapes is involved in multiple stresses and plays a central role in stress-induced and stress-tolerance.
 Baker, S.S., Wilhelm, K.S. and Thomashow, M.F. (1994) The 5’-Region of Arabidopsis thaliana cor15a Has Cis-Acting Elements That Confer Cold-, Drought- and ABA-Regulated Gene Expression. Plant Molecular Biology, 24, 701-713.
 Artus, N.N., et al. (1996) Constitutive Expression of the Cold Regulated Arabidopsis thaliana COR15a Gene Affects Both Chloroplast and Protoplast Freezing Tolerance. Proceedings of the National Academy of Sciences, 93, 13404-13409.
 Cattivelli, L. and Bartels, D. (1990) Molecular Cloning and Characterization of Cold-Regulated Genes in Barley. Plant Physiology, 93, 1504-1510.
 Crosatti, C., Polverino, L.P., Bassi, R. and Cattivelli, L. (1999) The Interaction between Cold and Light Controls the Expression of the Cold-Regulated Barley Gene Cor14b and the Accumulation of the Corresponding Protein. Plant Physiology, 119, 671-680.
 Crosatti, C., et al. (2003) Genetic Analysis of the Expression of the Cold Regulated Gene cor14b: A Way toward the Identification of Components of the Cold Response Signal Transduction in Triticeae. Canadian Journal of Botany, 81, 1162-1167.
 Rapacz, M., Wolanin, B., Hura, K. and Tyrka, M. (2008) The Effects of Cold Acclimation on Photosynthetic Apparatus and the Expression of COR14b in Four Genotypes of Barley (Hordeum Vulgare) Contrating in Their Tolerance to Freezing and High-Light Treatment in Cold Conditions. Annals of Botany, 101, 689-699.
 Shimamura, C., Ohno, R., Nakamura, C. and Takumi, S. (2006) Improvement of Freezing Tolerance in Tobacco Plants Expressing a Cold-Responsive and Chloroplast-Targeting Protein WCOR15 of Wheat. Journal of Plant Physiology, 1, 213-219.
 Li, X.W., et al. (2010) A novel Cold-Regulated Gene from Camellia sinensis, CsCOR1, Enhances Salt- and Dehydration-Tolerance in Tobacco. Biochemical and Biophysical Research Communications, 394, 354-359.
 Yamaguchi-Shinozaki, K. and Shinozaki, K. (1994) A Novel Cis-Acting Element in an Arabidopsis Gene Is Involved in Responsiveness to Drought, Low-Temperature, or High-Salt Stress. The Plant Cell, 6, 251-264.
 Stockinger, E.J., Gilmour, S.J. and Thomashow, M.F. (1997) Arabidopsis thaliana CBF1 Encodes an AP2 Domain-Containing Transcriptional Activator That Binds to the C-repeat/DRE, a Cis-Acting DNA Regulatory Element That Stimulates Transcription in Response to Low Temperature and Water Deficit. Proceedings of the National Academy of Sciences of the United States of America, 94, 1035-1040.
 Lagonigro, M.S., et al. (2004) CTAB-Urea Method Purifies RNA from Melanin for cDNA Microarray Analysis. Pigment Cell Research, 17, 312-315.
 Liang, C., et al. (2011) A Novel Cold-Regulated Gene, COR25, of Brassica napus Is Involved in Plant Response an Tolerance to Cold Stress. Plant Cell Reports, 30, 463-471.
 Steponkus, P.L., Uemura, M., Joseph, R.A., Gilmour, S.J. and Thomashow, M.F. (1998) Mode of Action of the COR15a Gene on the Freezing Tolerance of Arabidopsis thaliana. Proceedings of the National Academy of Sciences of the United States of America, 95, 14570-14575.
 Ruelland, E., Vaultier, M.N. and Hurry, A and Za, V. (2009) Cold Signalling and Cold Acclimation in Plants. Advances in Botanical Research, 49, 35-150.
 Dong, C., et al. (2013) Isolation and Expression Analysis of CBF4 from Vitis amurensis Associated with Stress. Agricultural Sciences, 4, 224-229.