Changes in the Physico-Chemical Properties of Human Kidney Cell Membranes during the Cancer Transformation
Affiliation(s)
Institute of Chemistry, University in Bialystok, Bialystok, Poland. Laboratory of Electrochemical Power Sources, Faculty of Chemistry, University of Warsaw, Warsaw, Poland. Clinical Department of Urology, Medical University of Bialystok, Bialystok, Poland.
Institute of Chemistry, University in Bialystok, Bialystok, Poland. Laboratory of Electrochemical Power Sources, Faculty of Chemistry, University of Warsaw, Warsaw, Poland. Clinical Department of Urology, Medical University of Bialystok, Bialystok, Poland.
ABSTRACT
Kidney tissue is particularly susceptible to reactive oxygen species attack which leads to development of cancer. During oxidative stress, membrane lipids and proteins are major targets of re-active oxygen species (ROS). This work is focused on changes of phospholipids, proteins content and electric charge that occur in cell membranes of kidney cancer of pT3 stage, grade G3 and with metastasis. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the human kidney cell membrane. It was shown that the process of cancer transformation was accompanied by an increase phospholipid levels and altered the level of integral proteins as determined by decrease phenylalanine, tyrosine, cysteine and arginine. Moreover, the process of cancer transformation significantly enhanced changes in the surface charge density of the human kidney cell membrane. Cell membrane structure and function are modified by neoplasm lesion.
Kidney tissue is particularly susceptible to reactive oxygen species attack which leads to development of cancer. During oxidative stress, membrane lipids and proteins are major targets of re-active oxygen species (ROS). This work is focused on changes of phospholipids, proteins content and electric charge that occur in cell membranes of kidney cancer of pT3 stage, grade G3 and with metastasis. Qualitative and quantitative phospholipid composition and the presence of integral membrane proteins were determined by high-performance liquid chromatography. Electrophoresis was used to determine the surface charge density of the human kidney cell membrane. It was shown that the process of cancer transformation was accompanied by an increase phospholipid levels and altered the level of integral proteins as determined by decrease phenylalanine, tyrosine, cysteine and arginine. Moreover, the process of cancer transformation significantly enhanced changes in the surface charge density of the human kidney cell membrane. Cell membrane structure and function are modified by neoplasm lesion.
Cite this paper
Szachowicz-Petelska, B. , Dobrzyńska, I. , Figaszewski, Z. and Kudelski, J. (2014) Changes in the Physico-Chemical Properties of Human Kidney Cell Membranes during the Cancer Transformation. Advances in Biological Chemistry, 4, 223-231. doi: 10.4236/abc.2014.44027.
Szachowicz-Petelska, B. , Dobrzyńska, I. , Figaszewski, Z. and Kudelski, J. (2014) Changes in the Physico-Chemical Properties of Human Kidney Cell Membranes during the Cancer Transformation. Advances in Biological Chemistry, 4, 223-231. doi: 10.4236/abc.2014.44027.
References
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[1] Szachowicz-Petelska, B., Dobrzyńska, I., Skrodzka, M., Darewicz, B., Figaszewski, Z.A. and Kudelski, J. (2013) Phospholipid Composition and Electric Charge in Healthy and Cancerous Parts of Human Kidneys. Journal of Membrane Biology, 246, 421-425.
http://dx.doi.org/10.1007/s00232-013-9554-7 [2] Szachowicz-Petelska, B., Sulkowski, S. and Figaszewski, Z. (2007) Altered Membrane Free Unsaturated Fatty Acid Composition in Human Colorectal Cancer Tissue. Molecular and Cellular Biochemistry, 294, 237-242.
http://dx.doi.org/10.1007/s11010-006-9264-x [3] Field, C.J., Thomson, C.A., van Aerde J.E., et al. (2000) Lower Proportion of CD45R0+ cells and Deficient Interleukin-10 Production by Formula-Fed Infants, Compared with Human-fed, is Corrected with Supplementation of Long-Chain Polyunsaturated Fatty Acids. Journal of Pediatric Gastroenterology and Nutrition, 31, 291-299.
http://dx.doi.org/10.1097/00005176-200009000-00017 [4] de Pablo, M.A. and Alvarez, D.C. (2000) Modulatory Effects of Dietary Lipids on Immune System Functions. Immunology and Cell Biology, 78, 31-39.
http://dx.doi.org/10.1046/j.1440-1711.2000.00875.x [5] Szachowicz-Petelska, B., Dobrzyńska, I., Figaszewski, Z. and Sulkowski, S. (2002) Changes in Physico-Chemical Properties of Human Large Intestine Tumour Cells Membrane. Molecular and Cellular Biochemistry, 238, 41-47.
http://dx.doi.org/10.1023/A:1019946718876 [6] Dobrzyńska, I., Szachowicz-Petelska, B., Sulkowski, S. and Figaszewski, Z.A. (2005) Changes in Electric Charge and Phospholipids Composition in Human Colorectal Cancer Cells. Molecular and Cellular Biochemistry, 276, 113-119.
http://dx.doi.org/10.1007/s11010-005-3557-3 [7] Szachowicz-Petelska, B., Sulkowski, S. and Figaszewski, Z.A. (2012) Altered Membrane Amino Acids Composition in Human Colorectal Cancer Tissue. Central European Journal of Chemistry, 10, 1245-1252.
http://dx.doi.org/10.2478/s11532-012-0050-1 [8] Ipata, P.L. (1967) A Coupled Optical Enzyme Assay for 5’-Nucleotidase. Analytical Biochemistry, 20, 30-36.
http://dx.doi.org/10.1016/0003-2697(67)90261-8 [9] Evans, W.H. (1970) Fractionation of Liver Plasma Membranes Prepared by Zonal Centrifugation. Biochemistry Journal, 166, 833-842. [10] Folch, J., Lees, M. and Stanley, G.H.S. (1957) A Simple Method for the Isolation and Purification of Total Lipids from Animal Tissues. Journal of Biological and Chemistry, 226, 497-509. [11] Tani, H., Kamidate, T. and Watanabe, H. (1997) Micelle-Mediated Extraction. Journal of Chromatography A, 780, 229-241.
http://dx.doi.org/10.1016/S0021-9673(97)00345-2 [12] Persaud, D.R., Persaud, D.G., Dalgleish, L., Nadeau, L. and Gauthier, S. (2000) Isolation and Purification of Serum and Interfacial Peptides of a Trypsinolyzed Beta-Lactoglobulin Oil-In-Water Emulsion. Journal of Chromatography B: Biomedical Sciences and Applications, 744, 389-397.
http://dx.doi.org/10.1016/S0378-4347(00)00266-8 [13] Krysiński, P. and Tien, H.Y. (1986) Membrane Electrochemistry. Progress in Surface Science, 23, 317-412.
http://dx.doi.org/10.1016/0079-6816(86)90016-X [14] Barrow, G.M. (1996) Physical Chemistry. McGraw-Hill Pocket Books, New York. [15] Dobrzyńska, I., Skrzydlewska, E. and Figaszewski, Z.A. (2006) Parameters Characterizing Acid-Base Equilibria between Cell Membrane and Solution and Their Application to Monitoring the Effect of Various Factors on the Membrane. Bioelectrochemistry, 69, 142-147.
http://dx.doi.org/10.1016/j.bioelechem.2006.01.004 [16] Takabe, W., Niki, E., Uchida, K., Yamada, S., Satoh, K. and Noguchi, N. (2001) Oxidative Stress Promotes the Development of Transformation: Involvement of a Potent Mutagenic Lipid Peroxidation Product, Acrolein. Carcinogenesis, 22, 935-941.
http://dx.doi.org/10.1093/carcin/22.6.935 [17] Toyokuni, S., Uchida, K., Okamato, K., Hattori-Nakakui, Y., Hiai, H. and Stadtman, E.R. (1994) Formation of 4-Hydroxy-2-Nonenal-Modified Proteins in the Renal Proximal Tubules of Rats Treated with a Renal Carcinogen, Ferric Nitrilotriacetate. Proceedings of the Naional Academy of Sciences of the United States of America, 91, 2616-2620.
http://dx.doi.org/10.1073/pnas.91.7.2616 [18] Uchida, K., Fukuda, A., Kawakishi, S., Hiai, H. and Toyokuni, S. (1995) A Renal Carcinogen Ferric Nitrilotriacetate Mediates a Temporary Accumulation of Aldehydes-Modified Proteins within Cytosolic Compartment of Rat Kidney. Archives of Biochemistry and Biophysics Arch, 317, 405-411.
http://dx.doi.org/10.1006/abbi.1995.1181 [19] Uchida, K. (2003) 4-Hydroksy-2-Nonenal: A Product and Mediator of Oxidative Stress. Progress in Lipid Research, 42, 318-343.
http://dx.doi.org/10.1016/S0163-7827(03)00014-6 [20] Pizzimenti, S., Toaldo, C., Pettazzoni, P., Ciamporcero, E., Dianzani, M.U. and Barrera, G. (2012) Lipid Peroxidation in Colorectal Carcinogenesis: Bad and Good News. In: Rajunor, E., Ed., Colorectal Cancer Biology, InTech, 155-172. [21] Esterbauer, H., Schaur, R.J. and Zollner, H. (1991) Chemistry and Biochemistry of 4-Hydroxynonenal, Malonaldehyde and Related Aldehydes. Free Radical Biology and Medicine, 11, 81-128.
http://dx.doi.org/10.1016/0891-5849(91)90192-6 [22] Isom, A.L., Barnes, S., Wilson, L., Kirk, M., Coward, L. and Darley-Usmar, V. (2004) Modification of Cytochrome c by 4-Hydroxy-2-Nonenal Evidence for Histidine, Lysine and Arginine-Aldehyde Adducts. The Journal of the American Society for Mass Spectrometry, 15, 1136-1147.
http://dx.doi.org/10.1016/j.jasms.2004.03.013 [23] Boirie, Y., Albright, R., Bigelow, M. and Nair, K.S. (2004) Impairment of Phenylalanine Conversion to Tyrosine Inend-Stage Renal Disease Causing Tyrosine Deficiency. Kidney International, 66, 591-596.
http://dx.doi.org/10.1111/j.1523-1755.2004.00778.x [24] Gius, D. (2004) Redox-Sensitive Signaling Factors and Antioxidants: How Tumor Cells Respond to Ionizing Radiation. American Society for Nutritional Sciences, 134, 3213S-3214S. [25] Gabai, V.L., Kabakov, A.E., Makarova, I.M., Mosina, V.A. and Mosin, A.F. (1994) Fragmentation of Ehrlich Ascites Carcinoma DNA during Influences Causing Aggregation of Cytoskeletal Proteins. Biokhimiia, 59, 551-558. [26] Rantanen, K., Pursiheimo, J., Högel, H., Himanen, V., Metzen, E. and Jaakkola, P.M. (2008) Prolyl Hydroxylase PHD3 Activates Oxygen-Dependent Protein Aggregation. Molecular Biology of the Cell, 19, 2231-2240.
http://dx.doi.org/10.1091/mbc.E07-11-1124 [27] Wang, P.H. (2005) Altered Glycosylation in Cancer: Sialic Acids and Sialyltransferases. Journal of Cancer Molecules 1, 73-81.