OJVM  Vol.4 No.6 , June 2014
Comparison of the Serum Proteins and Immune Responses of Velogenic Newcastle Disease Virus Infected Chickens and Ducks
Abstract: The effect of velogenic Newcastle disease virus (vNDV) on the immune responses and serum proteins was investigated in six-week-old ducks and chickens. Results showed that weight loss was markedly significant (p < 0.05) from days 3 - 21 (PI) in chickens and mild (p < 0.05) on days 3 and 15 PI in ducks. The antibody response obtained showed significant (p < 0.05) increase in infected chickens (IC) than those of the infected ducks (ID). While the total serum protein and serum globulin increased significantly (p < 0.05) in IC on days 7 and 14 PI, they decreased significantly (p < 0.05) in ID only on day 21 PI. The immune responses and serum protein values in this experiment X-ray showed less susceptibility of ducks when compared with the chickens. This may be related to marked anorexia and severe dehydration observed in the latter consequent upon serum concentration. Ducks could be maintaining the endemicity of Newcastle disease (ND) as reservoir host.
Cite this paper: Eze, C. , Shoyinka, V. , Okoye, J. , Ezema, W. , Ogbonna, I. , Eze, D. , Okwor, E. and Ikejiofor, O. (2014) Comparison of the Serum Proteins and Immune Responses of Velogenic Newcastle Disease Virus Infected Chickens and Ducks. Open Journal of Veterinary Medicine, 4, 122-128. doi: 10.4236/ojvm.2014.46014.

[1]   Kaslow, E.J. (2011) Serum Proteins and Functions. Califonia (800), 633-2322.

[2]   Eckersall, P.D. (2008) Proteins Proteomics and the Dysproteinemias. In: Kaneko, J.J., Harvey, J.W. and Bruss, M.L., Eds., Clinical Biochemistry of Domestic Animals, 6th Edition, Academic Press, San Diego, 117-155.

[3]   Schmidt, E.M.S., Paulillo, A.C., Locatelli-Dittrich, R., Beltrame, O. and Denadai, J. (2009) Serum Protein Profiles of Ring-Necked Pheasants Vaccinated or Not against Newcastle Disease. International Journal of Poultry Science, 8, 359-362.

[4]   Alexander, D.J. and Gough, R.E. (2003) Newcastle Disease and Other Avian Paramyxovirus Infections. In: Saif, Y.M., Barnes, H.J., Glisson, J.R., Fadly, A.M., McDougal, L.R. and Swayne, D.E., Eds., Disease of Poultry, 11th Edition, Iowa State University Press, Ames, 63-87.

[5]   Terregino, C., Cattoli, G., Grossele, B., Bertoli, E., Tisato, E. and Capua, I. (2003) Characterization of Newcastle Disease Virus Isolates Obtained from Eurasian Collared Doves (Streptopelia decaocto) in Italy Short Communication. Avian Pathology, 32, 63-68. 945021000070732

[6]   Vidanovic, D., Sekler, M., Asanin, R., Milic, N., Nisavic, J., Petrovic, T. and Savic, V. (2011) Characterization of Ve-logenic Newcastle Disease Viruses Isolated from Dead Wild Birds in Serbia during 2007. Journal of Wildlife Diseases, 47, 433-444.

[7]   Office International des Epizooties (OIE) (2009) Manual of Diagnostic Tests and Vaccines for Terrestial Animals. Newcastle Disease. OIE Standard Commission Publication, Office International Epizooties, 2009 Version, Part 2, Section 2.1, Chapter 2.3.14, 576-589.

[8]   Leslie, J. (2000) Newcastle Disease: Outbreak Losses and Control Policy Costs. Veterinary Record (Journal of British Veterinary Association), 146, 603-606.

[9]   Igwe, A.O. (2009) Comparative Study of the Pathogenicity and Pathogenesis of a Local Nigerian Velogenic Newcastle Disease Virus in Guinea Fowls and Chickens. MSc Dissertation, University of Nigeria, Nsukka, 1-112.

[10]   Alexander, D.J., Bell, J.G. and Alders, R.G. (2004) Newcastle Disease A Technology Review: With Special Emphasis on Its Effects on Village Chickens FAO Animal Production and Health. Paper 161 Food and Agriculture Organization of the United Nations, Rome.

[11]   Echeonwu, G.O.N., Ireogbu, C.U. and Emeruwa, A.C. (1993) Recovery of Velogenic Newcastle Disease Virus from Dead and Healthy Free Roaming Birds in Nigeria. Avian Pathology, 22, 383-387.

[12]   Okoye, J.O.A., Agu, A.O., Chineme, C.N. and Echeonwu, G.O.N. (2000) Pathological Characterization in Chicken of a Velogenic Newcastle Disease Virus Isolate from Guinea Fowl. Revue d’élevage et de Médecine Vétérinaire des pays Tropicaux, 53, 325-330.

[13]   Beard, C.W. (1980) Isolation and Identification of Avian Pathogens. 2nd Edition, American Association of Avian Pa-thologists, 129-135.

[14]   Villegas , P. and Purchase, H.G. (1989) Titration of Biological Suspension. In: A Laboratory Manual for the Isolation and Identification of Avian Pathogens, USA Kendal Hunt American Association of Avian Pathologists, Iowa, 186-190

[15]   Dumas, B.T. (1971) Diagnostic Reagent Kit for in Vitro Determination of Total Protein and Albumin in Serum (Code No25931). Clinical Chemistry Acta, 31, 87-96.

[16]   Piacenti, A.M., King, D.J., Seal, B.S., Zhang, J. and Brown, C.C. (2006) Pathogenesis of Newcastle Disease in Com-mercial and Specific Pathogen-Free Turkeys Experimentally Infected with Isolates of Different Virulence. Journal of Veterinary Pathology, 43, 168-178.

[17]   Mishra, S., Kataria, J.M., Verma, K.C. and Sah, R.L. (2000) Response of Chickens to Infection with Newcastle Disease Virus Isolated from a Guinea Fowl. Tropical Animal Health and Production, 32, 276-284.

[18]   Oladele, S.B., Abdu, P., Nok, A.J., Esievo, K.A.N. and Useh, N.M. (2005) Haemagglutination Inhibition Antibodies rectal Temperature and Total Protein of Chicken Infected with a Local Nigerian Isolate of Velogenic Newcastle Disease Virus. Veterinary Research Communication, 29, 171-179.

[19]   Scott, T.R. (2004) Our Current Understanding of Humoral Immunity of Poultry. Poultry Science, 83, 574-579.

[20]   Dortmans, J.C.F.M., Koch, G., Rottier, P.J.M. and Peeters, B.P.H. (2011) Virulence of Newcastle Disease Virus: What Is Known So Far? Veterinary Research, 42, 122.

[21]   Grogan, K.B., Fernandez, R.J., Barranon, R.F.J. and Espinosa, H.G. (2008) Avian Immune System: A Brief Review. Merial Select, Gainesville, 1-12.

[22]   Nester, W.E., Anderson, G.D., Evans, C.R., Pearsall, N.N., Nester M.T. and Hurley, D. (2004) Microbiology: A Human Perspective. McGraw Hill, New York.

[23]   Talaro, K.P. (2005) Foundation in Microbiology. 5th Edition, The McGraw Hill Companies, New York.

[24]   Filipovic, N., Stojevic, Z., Milinkovic-Tur, S., Ljubic, B.B. and Zdelar-Tuk, M. (2007) Changes in Concentration and Fractions of Blood Serum Proteins of Chickens during Fattening. Veterinarski Archiv, 77, 319-326.

[25]   Ihedioha, J.I. and Chineme, C.N. (2005) Fundamental of Systemic Veterinary Pathology. Vol. 2, Great A P Express, Nsukka.

[26]   Nnadi, P.A., Eze, P.C. and Ezema, W.S. (2010) Influence of Delayed Feeding on the Performance Development and Response of Immune System to Newcastle Disease Vaccination in Chickens. International Journal of Poultry Science, 9, 669-674.

[27]   Daramola, J., Adeboye, A., Fatoba, T. and Soladoye, A.O. (2003) Hematological and Biochemical Parameters of the West African Dwarf Goat. Department of Animal Production University of Ilorin Nigeria, Longman, Singapore City.

[28]   Lumeij, J.T. (2008) Avian Clinical Biochemistry. In: Kaneko, J.J., Harvey, J.W. and Bruss, M.L., Eds., Clinical Bio-chemistry of Domestic Animals, 6th Edition, Academic Press, San Diego, 839-872.

[29]   Petersen, H.H., Nielsen, J.P. and Heegaard, P.M. (2004) Application of Acute Phase Protein Measurements in Veteri-nary Clinical Chemistry. Veterinary Research, 35, 163-187.

[30]   Thomson, R.G. (1984) General Veterinary Pathology.