A Blueberry Extract Supplemented Diet Partially Restores α-Synuclein-Dependent Lifespan Loss and Developmental Defects in Drosophila
ABSTRACT
Oxidative stress has been strongly associated with Parkinson disease (PD) aetiology. We investigated the effects of blueberry extract (BBE) supplementation on α-synuclein induced phenotypes in a Drosophila melanogaster model of PD. Enhanced α-synuclein expression in D. melanogaster dopaminergic (DA) neurons can reduce lifespan and we have performed longevity assays to measure the effects of BBE on D. melanogaster survival. Flies expressing α-synuclein in their DA neurons fed BBE had up to an 8 day, or 15%, greater median lifespan than those fed a standard control diet. In addition, BBE improved α-synuclein-induced developmental defects in the Drosophila eye. Our biometric analyses revealed that individuals fed BBE had less atypical ommatidia as well as an increased number of mechanosensory bristle cells than those fed a control diet. We propose that BBE, rich in naturally occurring antioxidants, promotes the survival of neurons in tissues with increased levels of α-synuclein through a protective cell survival mechanism.
KEYWORDS
Drosophila melanogaster, Blueberry Extract, α-Synuclein, Model of Parkinson Disease, Lifespan, Ommatidia Development
Drosophila melanogaster, Blueberry Extract, α-Synuclein, Model of Parkinson Disease, Lifespan, Ommatidia Development
Cite this paper
Lipsett, D. and Staveley, B. (2014) A Blueberry Extract Supplemented Diet Partially Restores α-Synuclein-Dependent Lifespan Loss and Developmental Defects in Drosophila. Advances in Parkinson's Disease, 3, 3-9. doi: 10.4236/apd.2014.32002.
Lipsett, D. and Staveley, B. (2014) A Blueberry Extract Supplemented Diet Partially Restores α-Synuclein-Dependent Lifespan Loss and Developmental Defects in Drosophila. Advances in Parkinson's Disease, 3, 3-9. doi: 10.4236/apd.2014.32002.
References
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http://dx.doi.org/10.1089/rej.2009.0877 [10] Neto, C.C. (2007) Cranberry and Blueberry: Evidence for Protective Effects against Cancer and Vascular Diseases. Molecular Nutrition & Food Research, 51, 652-664.
http://dx.doi.org/10.1002/mnfr.200600279 [11] Li, H., Chaney, S., Forte, M. and Hirsh, J. (2000) Ectopic G-Protein Expression in Dopamine and Serotonin Neurons Blocks Cocaine Sensitization in Drosophila melanogaster. Current Biology, 10, 211-214. http://dx.doi.org/10.1016/S0960-9822(00)00340-7 [12] Freeman, M. (1996) Reiterative Use of the EGF Receptor Triggers Differentiation of all Cell Types in the Drosophila Eye. Cell, 87, 651-660. http://dx.doi.org/10.1016/S0092-8674(00)81385-9 [13] Brand, A.H. and Perrimon, N. (1993) Targeted Gene Expression as a Means of Altering Cell Fates and Generating Dominant Phenotypes. Development, 118, 401-415. [14] Staveley, B.E., Phillips, J.P. and Hilliker, A.J. (1990) Phenotypic Consequences of Copper-Zinc Superoxide-Dismutase Overexpression in Drosophila melanogaster. Genome, 33, 867-872.
http://dx.doi.org/10.1139/g90-130 [15] Abramoff, M.D., Magalhaes, P.J. and Ram, S.J. (2004) Image Processing with ImageJ. Biophotonics International, 11, 36-42. [16] Krikorian, R., Shidler, M.D., Nash, T.A., Kalt, W., Vinqvist-Tymchuk, M.R., Shukitt-Hale, B., et al. (2010.) Blueberry Supplementation Improves Memory in Older Adults. Journal of Agricultural and Food Chemistry, 58, 3996-4000. http://dx.doi.org/10.1021/jf9029332 [17] Galli, R.L., Bielinski, D.F., Szprengiel, A., Shukitt-Hale, B. and Joseph, J.A. (2006) Blueberry Supplemented Diet Reverses age-Related Decline in Hippocampal HSP70 Neuroprotection. Neurobiol Aging, 27, 344-350. http://dx.doi.org/10.1016/j.neurobiolaging.2005.01.017 [18] Peng, C., Zuo, Y., Kwan, K.M., Liang, Y., Ma, K.Y., Chan, H.Y.E., et al. (2012) Blueberry Extract Prolongs Lifespan of Drosophila melanogaster. Experimental Gerontology, 47, 170-178.
http://dx.doi.org/10.1016/j.exger.2011.12.001 [19] Haywood, A.F.M. and Staveley, B.E. (2004) Parkin Counteracts Symptoms in a Drosophila Model of Parkinson’s Disease. BMC Neuroscience, 5, 14. http://dx.doi.org/10.1186/1471-2202-5-14 [20] Peng, C., Chan, H.Y., Huang, Y., Yu, H. and Chen, Z.Y. (2011) Apple Polyphenols Extend the Mean Lifespan of Drosophila melanogaster. Journal of Agricultural and Food Chemistry, 59, 2097-2106.
http://dx.doi.org/10.1021/jf1046267 [21] Li, Y.M., Chan, H.Y.E., Huang, Y. and Chen, Z.Y. (2007) Green Tea Catechins Upregulate Superoxide Dismutase and Catalase in Fruit Flies. Molecular Nutrition & Food Research, 51, 546-554.
http://dx.doi.org/10.1002/mnfr.200600238 [22] Peng, C., Chan, H.Y., Li, Y.M., Huang, Y. and Chen, Z.Y. (2009) Black Tea Theaflavins Extend the Lifespan of Fruit Flies. Experimental Gerontology, 44, 773-783.
http://dx.doi.org/10.1016/j.exger.2009.09.004
[1] de Moura, M.B., dos Santos, L.S. and Van Houten, B. (2010) Mitochondrial Dysfunction in Neurodegenerative Diseases and Cancer. Environmental and Molecular Mutagenesis, 51, 391-405. [2] Polymeropoulos, M.H., Lavedan, C., Leroy, E., Ide, S.E., Dehejia, A., Dutra, A., et al. (1997) Mutation in the Alpha-Synuclein Gene Identified in Families with Parkinson’s Disease. Science, 276, 2045-2047. http://dx.doi.org/10.1126/science.276.5321.2045 [3] Stefanis, L. (2012) Alpha-Synuclein in Parkinson’s Disease. Cold Spring Harbor Perspectives in Medicine, 2, Article ID: a009399. http://dx.doi.org/10.1101/cshperspect.a009399 [4] Schapira, A.H. and Jenner, P. (2011) Etiology and Pathogenesis of Parkinson’s Disease. Movement Disorders, 26, 1049-1055. http://dx.doi.org/10.1002/mds.23732 [5] Byers, B., Cord, B., Nguyen, H.N., Schule, B., Fenno, L., Lee, P.C., et al. (2011) SNCA Triplication Parkinson’s Patient’s iPSC-Derived DA Neurons Accumulate α-Synuclein and Are Susceptible to Oxidative Stress. PLoS One, 6, Article ID: e26159. http://dx.doi.org/10.1371/journal.pone.0026159 [6] Feany, M.B. and Bender, W.W. (2000) A Drosophila Model of Parkinson’s Disease. Nature, 404, 394-398. http://dx.doi.org/10.1038/35006074 [7] Wassef, R., Haenold, R., Hansel, A., Brot, N., Heinemann, S.H. and Hoshi, T. (2007) Methionine Sulfoxide Reductase A and a Dietary Supplement S-Methyl-L-Cysteine Prevent Parkinson’s-Like Symptoms. The Journal of Neuroscience, 27, 12808-12816.
http://dx.doi.org/10.1523/JNEUROSCI.0322-07.2007 [8] Todd, A.M. and Staveley, B.E. (2008) Pink1 Suppresses Alpha-Synuclein-Induced Phenotypes in a Drosophila Model of Parkinson’s Disease. Genome, 51, 1040-1046.
http://dx.doi.org/10.1139/G08-085 [9] Long, J.G., Gao, H.X., Sun, L.J., Liu, J.K. and Zhao-Wilson, X. (2009) Grape Extract Protects Mitochondria from Oxidative Damage and Improves Locomotor Dysfunction and Extends Lifespan in a Drosophila Parkinson’s Disease Model. Rejuvenation Research, 12, 321-331.
http://dx.doi.org/10.1089/rej.2009.0877 [10] Neto, C.C. (2007) Cranberry and Blueberry: Evidence for Protective Effects against Cancer and Vascular Diseases. Molecular Nutrition & Food Research, 51, 652-664.
http://dx.doi.org/10.1002/mnfr.200600279 [11] Li, H., Chaney, S., Forte, M. and Hirsh, J. (2000) Ectopic G-Protein Expression in Dopamine and Serotonin Neurons Blocks Cocaine Sensitization in Drosophila melanogaster. Current Biology, 10, 211-214. http://dx.doi.org/10.1016/S0960-9822(00)00340-7 [12] Freeman, M. (1996) Reiterative Use of the EGF Receptor Triggers Differentiation of all Cell Types in the Drosophila Eye. Cell, 87, 651-660. http://dx.doi.org/10.1016/S0092-8674(00)81385-9 [13] Brand, A.H. and Perrimon, N. (1993) Targeted Gene Expression as a Means of Altering Cell Fates and Generating Dominant Phenotypes. Development, 118, 401-415. [14] Staveley, B.E., Phillips, J.P. and Hilliker, A.J. (1990) Phenotypic Consequences of Copper-Zinc Superoxide-Dismutase Overexpression in Drosophila melanogaster. Genome, 33, 867-872.
http://dx.doi.org/10.1139/g90-130 [15] Abramoff, M.D., Magalhaes, P.J. and Ram, S.J. (2004) Image Processing with ImageJ. Biophotonics International, 11, 36-42. [16] Krikorian, R., Shidler, M.D., Nash, T.A., Kalt, W., Vinqvist-Tymchuk, M.R., Shukitt-Hale, B., et al. (2010.) Blueberry Supplementation Improves Memory in Older Adults. Journal of Agricultural and Food Chemistry, 58, 3996-4000. http://dx.doi.org/10.1021/jf9029332 [17] Galli, R.L., Bielinski, D.F., Szprengiel, A., Shukitt-Hale, B. and Joseph, J.A. (2006) Blueberry Supplemented Diet Reverses age-Related Decline in Hippocampal HSP70 Neuroprotection. Neurobiol Aging, 27, 344-350. http://dx.doi.org/10.1016/j.neurobiolaging.2005.01.017 [18] Peng, C., Zuo, Y., Kwan, K.M., Liang, Y., Ma, K.Y., Chan, H.Y.E., et al. (2012) Blueberry Extract Prolongs Lifespan of Drosophila melanogaster. Experimental Gerontology, 47, 170-178.
http://dx.doi.org/10.1016/j.exger.2011.12.001 [19] Haywood, A.F.M. and Staveley, B.E. (2004) Parkin Counteracts Symptoms in a Drosophila Model of Parkinson’s Disease. BMC Neuroscience, 5, 14. http://dx.doi.org/10.1186/1471-2202-5-14 [20] Peng, C., Chan, H.Y., Huang, Y., Yu, H. and Chen, Z.Y. (2011) Apple Polyphenols Extend the Mean Lifespan of Drosophila melanogaster. Journal of Agricultural and Food Chemistry, 59, 2097-2106.
http://dx.doi.org/10.1021/jf1046267 [21] Li, Y.M., Chan, H.Y.E., Huang, Y. and Chen, Z.Y. (2007) Green Tea Catechins Upregulate Superoxide Dismutase and Catalase in Fruit Flies. Molecular Nutrition & Food Research, 51, 546-554.
http://dx.doi.org/10.1002/mnfr.200600238 [22] Peng, C., Chan, H.Y., Li, Y.M., Huang, Y. and Chen, Z.Y. (2009) Black Tea Theaflavins Extend the Lifespan of Fruit Flies. Experimental Gerontology, 44, 773-783.
http://dx.doi.org/10.1016/j.exger.2009.09.004