JCDSA  Vol.4 No.3 , June 2014
Isolation of Anti Ageing Molecules Secreted by Human Gingival Stem Cells. Protection of Collagen and Elastic Networks

Skin aging results in an imbalance between synthesis and degradation of the extracellular matrix. Overproduction of degradative enzymes (MMPs) and oxygen free radicals during chronological and photo-induced aging leads a degradation of the network and elastic skin collagen. Our previous work demonstrated that a culture supernatant of gingival stem cells had anti-aging activities in vitro, ex vivo and in vivo in humans. However, this culture medium is very complex and currently not responding to the European cosmetics regulation. After the analysis of the culture medium we have isolated 4 molecules interesting in terms of skin anti-aging activity: TIMP1, Selenium, Folic Acid and Glycin (TSAG). After the study of the most efficiency concentration of each molecule, the composition of TSAG is tested on irradiated UVA human dermal fibroblast and human skin. TSAG preserves the elastin and collagen network and inhibits the MMP1, MMP3 and MMP9 activities. The advantage of this mimetic solution of stem cells is to be stable, easily reproducible and non-human origin. The outlook for cosmetology seems interesting.

Cite this paper: Cherifi, H. , Naveau, A. , Robert, L. and Gogly, B. (2014) Isolation of Anti Ageing Molecules Secreted by Human Gingival Stem Cells. Protection of Collagen and Elastic Networks. Journal of Cosmetics, Dermatological Sciences and Applications, 4, 156-165. doi: 10.4236/jcdsa.2014.43022.

[1]   Rafferty, T.S., Green, M.H., Lowe, J.E., Arlett, C., Hunter, J.A., Beckett, G.J. and McKenzie, R.C. (2003) Effects of Selenium Compounds on Induction of DNA Damage by Broadband Ultraviolet Radiation in Human Keratinocytes. British Journal of Dermatology, 148, 1001-1009.

[2]   Gogly, B., Ferré, F., Cherifi, H., Naveau, A. and Fournier, B. (2011) Inhibition of Elastin and Collagen Networks Degradation in Human Skin by Gingival Fibroblast. In Vitro, ex Vivo and in Vivo Studies. Journal of Cosmetics, Dermatological Sciences and Applications, 1, 4-14.

[3]   Fournier, B., Ferré, F., Couty, L., Lataillade, J.J., Gourven, M., Naveau, A., Coulom, B., Lafont, A. and Gogly, B. (2010) Multipotent Progenitor Cells in Gingival Connective Tissue. Tissue Engineering Part A, 16, 2891-2899.

[4]   Fournier, B., Larjava, H. and Hakkinen, L. (2013) Gingiva as a Source of Stem Cells with Therapeutic Potential. Stem Cells and Development, 22, 3157-3177.

[5]   Naveau, A., Lataillade, J.J., Fournier, B.P., Couty, L., Prat, M., Ferré, F.C., Gourven, M., Durand, E., Coulomb, B., Lafont, A. and Gogly, B. (2010) Phenotypic Study of Human Gingival Fibroblasts in a Medium Enriched with Platelet Lysate. Journal of Periodontology, 82, 632-641.

[6]   Hornebeck, W. (2003) Down-Regulation of Tissue Inhibitor of Matrix Metalloprotease-1 (TIMP-1) in Aged Human Skin Contributes to Matrix Degradation and Impaired Cell Growth and Survival. Pathologie Biologie, 51, 569-573.

[7]   Rittié, L. and Fisher, G.J. (2002) UV-Light-Induced Signal Cascades and Skin Aging. Ageing Research Reviews, 1, 705-720.

[8]   Emonet-Piccardi, N., Richard, M.J., Ravanat, J.L., Signorini, N., Cadet, J. and Béani, J.C. (1998) Protective Effects of Antioxidants against UVA-Induced DNA Damage in Human Skin Fibroblasts in Culture. Free Radical Research, 29, 307-313.

[9]   McKenzie, R.C. (2000) Selenium, Ultraviolet Radiation and the Skin. Clinical and Experimental Dermatology, 25, 631-636.

[10]   Stadtman, T.C. (2000) Selenium Biochemistry. Mammalian Selenoenzymes. Annals of the New York Academy of Sciences, 899, 399-402.

[11]   Emonet, N., Leccia, M.T., Favier, A., Beani, J.C. and Richard, M.J. (1997) Thiols and Selenium: Protective Effect on Human Skin Fibroblasts Exposed to UVA Radiation. Journal of Photochemistry and Photobiology B, 40, 84-90.

[12]   Rafferty, T.S., McKenzie, R.C., Hunter, J.A., Howie, A.F., Arthur, J.R., Nicol, F. and Beckett, G.J. (1998) Differential Expression of Selenoproteins by Human Skin Cells and Protection by Selenium from UVB-Radiation-Induced Cell Death. Biochemical Journal, 332, 231-236.

[13]   Rafferty, T.S., Walker, C., Hunter, J.A., Beckett, G.J. and McKenzie, R.C. (2002) Inhibition of Ultraviolet B Radiation-Induced Interleukin 10 Expression in Murine Keratinocytes Byselenium Compounds. British Journal of Dermatology, 146, 485-489.

[14]   Moysan, A., Morlière, P., Marquis, I., Richard, A. and Dubertret, L. (1995) Effects of Selenium on UVA-Induced Lipid Peroxidation in Cultured Human Skin Fibroblasts. Skin Pharmacology and Physiology, 8, 139-148.

[15]   Thorling, E.B., Overvad, K. and Bjerring, P. (1983) Oral Selenium Inhibits Skin Reactions to UV Light in Hairless Mice. Acta Pathologica et Microbiologica Scandinavica A, 91, 81-83.

[16]   Overvad, K. (1998) Selenium and Cancer. Bibliotheca Nutritio et Dieta, 54, 141-149.

[17]   Reid, M.E., Duffield-Lillico, A.J., Slate, E., Natarajan, N., Turnbull, B., Jacobs, E., Combs Jr., G.F., Alberts, D.S., Clark, L.C. and Marshall, J.R. (2008) The Nutritional Prevention of Cancer: 400 mcg per Day Selenium Treatment. Nutrition and Cancer, 60, 155-163.

[18]   Pinnell, S.R. (2003) Cutaneous Photodamage, Oxidative Stress, and Topical Antioxidant Protection. Journal of the American Academy of Dermatology, 48, 1-19.

[19]   Puizina-Ivic, N., Miric, L., Carija, A., Karlica, D. and Marasovic, D. (2010) Modern Approach to Topical Treatment of Aging Skin. Collegium Antropologicum, 34, 1145-1153.

[20]   Fischer, F., Achterberg, V., Marz, A., Puschmann, S., Rahn, C.D., Lutz, V., Krüger, A., Schwengler, H., Jaspers, S., Koop, U., Blatt, T., Wenck, H. and Gallinat, S. (2011) Folic Acid and Creatine Improve the Firmness of Human Skin in Vivo. Journal of Cosmetic Dermatology, 10, 15-23.

[21]   Pickart, L. (2008) The Human Tri-Peptide GHK and Tissue Remodeling. Journal of Biomaterials Science, Polymer Edition, 19, 969-988.