JBPC  Vol.5 No.2 , May 2014
Gibbs-Donnan Potential as a Tool for Membrane Vesicles Polarization
Abstract: It has been theoretically predicted that under conditions leading to Gibbs-Donnan equilibrium in case when size of one compartment is very different from another (as in system “membrane vesicle/liposomes—incubation medium”) stable transmembrane potential can be formed, which value is sufficient to fit requirement of real transmembrane potential. Four partial cases were considered with different location and charge of impermeable ion and it was concluded that locations of impermeable ions in medium provide stable transmembrane potential with sufficient value of 60 - 70 mV. Potential-sensitive probe, such as DiOC6(3) and oxonol VI, were used to confirm the calculated potential. According to the change in fluorescence level and emission/excitation shift, a stable and relatively high transmembrane potential can be formed if salt of impermeable ion is located in incubation medium. Impermeable cations and anions may be used to create positive and negative transmembrane potential respectively.
Cite this paper: Iuliia, M. , Sergiy, K. , Tetyana, V. and Oleksandr, S. (2014) Gibbs-Donnan Potential as a Tool for Membrane Vesicles Polarization. Journal of Biophysical Chemistry, 5, 78-89. doi: 10.4236/jbpc.2014.52009.

[1]   Sunose, H., Ikeda, K., Saito, Y., Nishiyama, A. and Takasaka, T. (1992) Membrane Potential Measurement in Isolated Outer Hair Cells of the Guinea Pig Cochlea Using Conventional Microelectrodes. Hearing Research, 62, 237-244.

[2]   Berggren, P. and Sohtell, M. (1986) Microelectrode Studies of D-Glucose- and K+-Induced Changes in Membrane Potential of Electrofused Insulin-Producing Cells. FEBS Letters, 202, 367-372.

[3]   Nobes, C.D. and Brand, M.D. (1989) A Quantitative Assessment of the Use of 36Cl- Distribution to Measure Plasma Membrane Potential in Isolated Hepatocytes. BBA—Biomembranes, 987, 115-123.

[4]   Cuevas, J. (2007) The Resting Membrane Potential. In: Enna, S.J., Bylund, D.B., Eds., xPharm: The Comprehensive Pharmacology Reference, Elsevier Inc., New York, 1-4.

[5]   Hodgkin, A.L. and Huxley, A.F. (1945) Resting and Action Potentials in Single Nerve Fibres. The Journal of Physiology, 104, 176-195.

[6]   Andersen, S.S.L., Jackson, A.D. and Heimburg, T. (2009) Towards a Thermodynamic Theory of Nerve Pulse Propagation. Progress in Neurobiology, 88, 104-113.

[7]   Guiet-Bara, A., Ibrahim, B., Leveteau, J. and Bara, M. (1990) Calcium Channels, Potassium Channels and Membrane Potential of Smooth Muscle Cells of Human Allantochorial Placental Vessels. Bioelectrochem. Bioenergetics, 48, 407-413.

[8]   Vamosi, G. (2006) The Role of Supramolecular Protein Complexes and Membrane Potential in Transmembrane Signaling Processes of Lymphocytes. Immunology Letters, 104, 53-58.

[9]   Chimerel, C., Field, C.M., Pinero-Fernandez, S., Keyser, U.F. and Summers, D.K. (2012) Indole Prevents Escherichia coli Cell Division by Modulating Membrane Potential. BBA—Biomembrane, 1818, 1590-1594.

[10]   Goudeau, H., Goudeau, M. and Guibourt, N. (1992) The Fertilization Potential and Associated Membrane Potential Oscillations during the Resumption of Meiosis in the Egg of the Ascidian Phallusia mammillata. Developmental Biology, 153, 227-241.

[11]   Khelashvili, G., Weinstein, H. and Harries, D. (2008) Protein Diffusion on Charged Membranes: A Dynamic Mean-Field Model Describes Time Evolution and Lipid Reorganization. Biophysical Journal, 94, 2580-2597.

[12]   Strahl, H. and Hamoen, L.W. (2010) Membrane Potential Is Important for Bacterial Cell Division. Proceedings of the National Academy of Sciences of the United States of America, 107, 12281-12286.

[13]   Kralj, J.M., Hochbaum, D.R., Douglass, A.D. and Cohen, A.E. (2011) Electrical Spiking in Escherichia coli Probed with a Fluorescent Voltage-Indicating Protein. Science, 333, 345-348.

[14]   Hell, J.W. and Jahn, R. (1998) Bioenergetic Characterization of γ-Aminobutyric Acid Transporter of Synaptic Vesicles. Methods in Enzymology, 296, 116-124.

[15]   Dudeja, P.K., Tyagi, S., Kavilaveettil, R.J., Gill, R. and Said, H.M. (2001) Mechanism of Thiamine Uptake by Human Jejunal Brush-Border Membrane Vesicles. American Journal of Physiology-Cell Physiology, 281, 786-792.

[16]   Krick, W., Wolff, N.A. and Burckhardt, G. (2000) Voltage-Driven p-Aminohippurate, Chloride, and Urate Transport in Porcine Renal Brush-Border Membrane Vesicles. Pflügers Archiv, 441, 125-132.

[17]   Malo, C. and Wilson, J.X. (2000) Glucose Modulates Vitamin C Transport in Adult Human Small Intestinal Brush Border Membrane Vesicles. Journal of Nutrition, 130, 63-69.

[18]   Zelikovic, I. and Budreau-Patters, A. (1999) Cl- and Membrane Potential Dependence of Amino Acid Transport across the Rat Renal Brush Border Membrane. Molecular Genetics and Metabolism, 67, 236-247.

[19]   Freel, R.W., Hatch, M. and Vaziri, N.D. (1998) Conductive Pathways for Chloride and Oxalate in Rabbit Ileal Brush-Border Membrane Vesicles. American Journal of Physiology, 275, 748-757.

[20]   Krick, W., Dolle, A., Hagos, Y. and Burckhardt, G. (1998) Characterization of the Chloride Conductance in Porcine Renal Brush-Border Membrane Vesicles. Pflügers Archiv, 435, 415-421.

[21]   Yanagawa, N., Jo, O.D. and Said, H.M. (1997) Riboflavin Transport by Rabbit Renal Brush Border Membrane Vesicles. Biochimica et Biophysica Acta, 1330, 172-178.

[22]   Bonisch, H. (1998) Transport and Drug Binding Kinetics in Membrane Vesicle Preparation. Methods in Enzymology, 296, 259-278.

[23]   Garaiová, Z., Mohsin, M., Vargová, V., Banica, F.-G. and Hianik, T. (2012) Complexation of Cytochrome c with Calixarenes Incorporated into the Lipid Vesicles and Supported Membranes. Bioelectrochemistry, 87, 220-225.

[24]   Barts, P.W.J.A. and Borst-Pauwels, G.W.F.H. (1985) Effects of Membrane Potential and Surface Potential on the Kinetics of Solute Transport. Biochimica et Biophysica Acta (BBA)—Biomembranes, 813, 51-60.

[25]   Zhan, H. and Lazaridis, T. (2012) Influence of the Membrane Dipole Potential on Peptide Binding to Lipid Bilayers. Biophysical Chemistry, 161, 1-7.

[26]   Babich, L.G., Fomin, V.P. and Kosterin, S.A. (1990) Effect of the Membrane Potential on the Mg2+,ATP-Dependent Transport of Ca2+ across Smooth Muscle Sarcolemma. Biohymia (in Russian), 55, 1890-1901.

[27]   Veklich, T.O. and Kosterin, S.A. (2005) Comparison Analysis of Myometrium Plasma Membrane Na+, K+-ATPase and Mg2+-ATPase Properties. Ukrain Biochemical Journal (in Ukrainian), 77, 66-75.

[28]   Bradford, M.M. (1976) A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Prinsiple of Protein-Dye Binding. Analytical Biochemistry, 72, 248-282.

[29]   Sperelakis, N. (2012) Gibbs-Donnan Equilibrium Potentials. In: Sperelakis, N., Ed., Cell Physiology Source Book, Cell Physiology Source Book, Inc., San-Diego, 147-151.

[30]   Kosterins, S.A. and Cherny, A.P. (1991) Gibbs-Donnan Equilibrium in the System Membrane Vesicles—Incubation Medium. Biophysica (in Russian), 36, 826-829.

[31]   Hoffman, J.F. and Laris, P.C. (1974) Determination of Membrane Potentials in Human and Amphiuma Red Blood Cells by Means of a Fluorescent Probe. The Journal of Physiology, 239, 519-552.

[32]   Apell, H.-J. and Bersch, B. (1987) Oxonol VI as an Optical Indicator for Membrane Potentials in Lipid Vesicles. BBA—Biomembranes, 903, 480-494.