carbon dioxide (CO2)-evolution from soils are important in
evaluating biomass and activity of soil microorganisms, as well as decomposition
of soil organic matter. The Respicond VI is a fully computerized system
allowing continuous measurement of CO2 evolution in short- and
long-term soil incubation experiments in up to 96 incubation vessels. The
measurement of CO2 evolution is based on the absorption of CO2 by an electrolyte (KOH solution) producing a change in the cell conductance
measured using two electrodes. In this study, the Respicond VI was recalibrated
yielding 174.5 mg CO2 as constant A expressing the theoretical maximum amount of CO2 absorbed in 10 ml 0.5 M KOH. This value of A corresponds to 34.9 mg CO2 ml-1 1 M KOH. The
constant A does neither depend on the
investigated incubation temperatures (5°C - 25°C) nor on the concentrations of
the KOH solutions (0.5, 0.1, 0.05 M KOH). To eliminate any influence of
changing incubation temperatures, either induced by uncertainties in
temperature control or as a part of the experimental setup, on the conductance
of KOH solution, a correction procedure was developed using a factor calculated
from changing conductance of KOH solutions in incubation vessels without soil.
Cite this paper
Smirnova, N. , Demyan, M. , Rasche, F. , Cadisch, G. and Müller, T. (2014) Calibration of CO2
Trapping in Alkaline Solutions during Soil Incubation at Varying Temperatures Using a Respicond VI. Open Journal of Soil Science
, 161-167. doi: 10.4236/ojss.2014.45019
 Anderson, J.P.E., Page, A.L., Miller, R.H. and Keeney, D.R. (1982) Soil Respiration. In: Page, A.L., Ed., Methods of Soil Analysis, Part 2, 2nd Edition, ASA and SSSA, Madison, 831-871.
 Cropper, W.P., Ewel, K.C. and Raich, J.W. (1985) The Measurement of Soil CO2 Evolution In-Situ. Pedobiologia, 28, 35-40.
 Sparling, G.P. and West, A.W. (1990) A Comparison of Gas Chromatography and Differential Respirometer Methods to Measure Soil Respiration and to Estimate the Soil Microbial Biomass. Pedobiologia, 34, 103-112.
 Bachelor, P.P., McIntyre, J.I., Amonette, J.E., Hayes, J.C., Milbrath, B.D. and Saripalli, P. (2008) Potential Method for Measurement of CO2 Leakage from Underground Sequestration Fields Using Radioactive Tracers. Journal of Radioanalytical and Nuclear Chemistry, 277, 85-89. http://dx.doi.org/10.1007/s10967-008-0713-8
 Chapman, S.B. (1971) A Simple Conductimetric Soil Respirometer for FIELD Use. Oikos, 22, 348-353. http://dx.doi.org/10.2307/3543857
 Anderson, J.M. and Ineson, P. (1982) A Soil Microcosm System and Its Application to Measurements of Respiration and Nutrient Leaching. Soil Biology and Biochemistry, 14, 415-416. http://dx.doi.org/10.1016/0038-0717(82)90015-3
 Nordgren, A. (1988.) Apparatus for Continuous, Long-Term Monitoring of Soil Respiration Rate in Large Numbers of Samples. Soil Biology and Biochemistry, 20, 955-957.
 Ilstedt, U., Nordgren, A. and Malmer, A. (2006) Soil Chemical and Microbial Properties after Disturbance by Crawler Tractors in a Malaysian Forest Plantation. Forest Ecology and Management, 225, 313-319. http://dx.doi.org/10.1016/j.foreco.2006.01.008
 Gnankambary, Z., Ilstedt, U., Nyberg, G., Hien, V. and Malmer, A. (2008) Nitrogen and Phosphorus Limitation of Soil Microbial Respiration in Two Tropical Agroforestry Parklands in the South-Sudanese Zone of Burkina Faso: The Effects of Tree Canopy and Fertilization. Soil Biology and Biochemistry, 40, 350-359. http://dx.doi.org/10.1016/j.soilbio.2007.08.015
 Hartley, I.P., Hopkins, D.W., Sommerkorn, M. and Wookey, P.A. (2010) The Response of Organic Matter Mineralisation to Nutrient and Substrate Additions in Sub-Arctic Soils. Soil Biology and Biochemistry, 42, 92-100. http://dx.doi.org/10.1016/j.soilbio.2009.10.004