AE  Vol.2 No.2 , April 2014
Raising of Horn Fly Haematobia irritans (L.) (Diptera: Muscidae) in Laboratory by Means of Egg and Larva Inoculation
ABSTRACT
Several studies have required Haematobia irritans (L.) raising in laboratory. The present study assessed two methods of inoculating immature forms of H. irritans to obtain adults. In 2007, 15 Nellore steers (Bos indicus) (L.) were used for the collection of feces free of anthelmintic treatment and flies to produce for eggs and larva. For method I, 30 eggs were incubated in square filter paper (5 × 5 cm) and deposited on bovine feces (500 g) where they were kept until hatching and spontaneous penetration of larvae (L1) into the fecal mass. After 24 h, eggs were analyzed under a stereoscope microscope (40×) for the number of larvae that instinctively penetrated the feces. In method II, larvae were obtained only by natural egg hatching. At birth, 30 larvae were collected and individually inoculated, directly onto the fecal plate by employing a moistened brush. The tests were carried out at controlled temperature (28°C ± 2°C) and saturated humidity (80%) until the emergence of flies with both methods. The number of emerged flies was considered in the result. Using method I, 276 (76.7%) flies emerged from 360 inoculated eggs, while using method II, 283 (78.6%) flies emerged from 360 inoculated larvae. There was no significant difference (P = 0.7821) between methods for the number of flies; however, the proportion between males and females by means of larva inoculation was different from 1:1 (P = 0.0146). Results indicated that both methods led to a satisfactory production of flies and egg inoculation provided an easier establishment.

Cite this paper
Lima, L. , Beloti, C. , Perri, S. , Kaneto, C. , Bresciani, K. and Prado, Â. (2014) Raising of Horn Fly Haematobia irritans (L.) (Diptera: Muscidae) in Laboratory by Means of Egg and Larva Inoculation. Advances in Entomology, 2, 76-80. doi: 10.4236/ae.2014.22013.
References
[1]   Glaser, R.W. (1924) Rearing Flies for Experimental Purposes, with Biological Notes. Journal of Economic Entomology, 17, 486-497.

[2]   Depner, K.R. (1965) Ultraviolet Irradiation of Cattle in Relation to Diapause in the Horn Fly, Haemarobia irrirtans (L.) (Diptera: Muscidae). International Journal of Biometeorology, 9, 167-170.
http://dx.doi.org/10.1007/BF02188472

[3]   Bay, D.E. and Harris, R.L. (1978) Small Scale Laboratory Rearing of the Horn Fly. Southwestern Entomologist, 3, 276-278.

[4]   Valiela, I. (1969) An Experimental Study of the Mortality Factors of Larval Musca autumnalis DeGeer. Ecological Monographs, 39, 199-225. http://dx.doi.org/10.2307/1950742

[5]   Danks, H.V. (2006) Short Life Cycles Insects and Mites. Canadian Entomologist, 138, 407-463. http://dx.doi.org/10.4039/n06-803

[6]   Depner, K.R. (1961) The Effect of Temperature on Development and Diapause of the Horn Fly, Shiphona irritas (L.) (Diptera: Muscidae). Canadian Entomologist, 93, 855-859. http://dx.doi.org/10.4039/Ent93855-10

[7]   Berry, I.L. and Kunz, S.E. (1978) Oviposition of Stable Flies in Responses to Temperature and Humidity. Environmental Entomology, 7, 213-216.

[8]   Dougherty, C.T. and Knapp, F.W. (1994) Oviposition and Development of Face Flies in Dung from Cattle on Herbage and Supplemented Herbage Diets. Veterinary Parasitolgy, 55, 115-127.
http://dx.doi.org/10.1016/0304-4017(94)90061-2

[9]   Berkebile, D.R., Sagel, A., Skoda, S.R. and Foster, J.E. (2006) Laboratory Environment Effects on the Reproduction and Mortality of Adult Screwworm (Diptera: Calliphoridae). Neotropical Entomology, 35, 781-786. http://dx.doi.org/10.1590/S1519-566X2006000600010

[10]   Kunz, S.E. (1980) Horn Fly Production as Affected by Seasonal Changes in Rangeland Forage Conditions. Southwestern Entomologist, 5, 80-83.

[11]   Thomas, P.L. and Davis, N.C. (1984) A Method for Rearing the Buffalo Fly, Haematobia irritans exigua de Meijere. Australian Journal of Entomology, 23, 81-82.
http://dx.doi.org/10.1111/j.1440-6055.1984.tb01913.x

[12]   Lysyk, T.J. (1999) Effect of Temperature on Time to Eclosion and Spring Emergence of Postdiapausing Horn Flies (Diptera: Muscidae). Environmental Entomology, 28, 387-397.

[13]   Anderson, J.M.E. (1995) Culturing Haematobia exigua de Meijere (Diptera: Muscidae) in the Laboratory. Australian Journal of Entomology, 34, 17-21. http://dx.doi.org/10.1111/j.1440-6055.1995.tb01268.x

[14]   Lohmeyer, K.H. and Kammlah, D.M. (2006) Improved Mass Rearing Techniques for the Horn Fly, Haematobia irritans (L.) (Diptera: Muscidae). Southwestern Entomologist, 31, 83-85.

[15]   Lima, L.G.F., Perri, S.H.V. and Prado, A.P. (2010) Porcentagem de Eclosão de Ovos de Haematobia irritans (L.) (Diptera: Muscidae) em Laboratório. Bioscience Journal, 26, 478-483.

[16]   Stegeman, D.A., Tozer, R.S. and Sutherst, R.W. (1996) Procedures for Mass Rearing the Buffalo Fly, Haematobia irritans exigua De Meijere (Diptera: Muscidae). Australian Journal of Entomology, 35, 77-79. http://dx.doi.org/10.1111/j.1440-6055.1996.tb01365.x

[17]   Gallardo, J.L., Moreno, D.S., Waldon, M. and Rodrigues, A.M. (2000) Mortalidad de la mosca del cuerno Haematobia irritans (L.) causada por el pigmento floxin Ba. Técnica Pecuaria en México, 38, 211-217.

[18]   Lima, L.G.F., Perri, S.H.V. and Prado, A.P. (2009) Efeito do tratamento em dose única ou múltipla com ivermectina na emergência de Haematobia irritans (L.) (Diptera: Muscidae). Veterinária e Zootecnia, 16, 410-418.

[19]   Kuramochi, K. and Hõji, H. (1984) Blood Meal Size of the Horn Fly (L.) (Diptera: Muscidae) Estimate by Measuring the Feeding Time. Research Bulletin of Obihiro University Series I, 13, 287-292.

[20]   Kuramochi, K. (1985) Rearing Method of Horn Fly Larvae with Freeze-Dried Bovine Dung (Diptera: Muscidae). Research Bulletin of Obihiro University Series I, 143, 305-309.

[21]   Perotti, M.A. and Lysyk, T.J. (2003) Novel Growth Media for Rearing Larval Horn Flies, Haematobia irritans (Diptera: Muscidae). Journal of Medical Entomology, 40, 22-29. http://dx.doi.org/10.1603/0022-2585-40.1.22

[22]   Smith, A.M. (1984) Larval Instar Determination and Temperature Development Studies of Immature Stages of the Common Armyworm, Mythimna convecta (Walker) (Lepidoptera: Noctuidae). Australian Journal of Entomology, 23, 91-97. http://dx.doi.org/10.1111/j.1440-6055.1984.tb01917.x

 
 
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