IJCM  Vol.5 No.9 , April 2014
Effectiveness of Laser Therapy (AlGaInP) on Cicatrization of Experimental Cutaneous Wound in Rats
ABSTRACT

The use of laser therapy is one of the most effective tools in the prevention and treatment of skin ulcers. It is an effective treatment choice because it potentiates the trophic regenerative, anti inflammatory, and painkiller effects. This study aimed to assess the effectiveness of low level laser therapy in treating induced cutaneous ulcers in rats. An experimental study using 12 female Wistar rats (Rattus novergicus), weighing between 150 and 250 g, from the room of animal husbandry at the Center of Health Sciences of UNIFOR. Rats underwent surgery to induce cutaneous ulcer. Subsequently, the animals were divided into two randomized groups: 1) Control group; and 2) Experimental group. They received nine applications of AlGaInP laser treatment (power of 30 mW) with an intensity of 4 J/cm2, using the scan method, for 15 seconds over the entire ulcer. The cicatrization process was evaluated from the 1st to 19th postoperative (PO) day, but on alternate days. The results showed that animals treated with laser had less area of cutaneous wound than the control group from the 7th until the 17th postoperative day, highlighting the 7th and the 9th PO with a significant reduction in wound area of 28.8% (p = 0.00) and 23,5% (p = 0.01), respectively. Treatment with the AlGaInP laser was effective in the repair of cutaneous wound when compared to the control group—particularly on the 7th postoperative day.


Cite this paper
dos Santos Vasconcelos, R. , Prado Vasconcelos, R. , Torres Monteiro Melo, L. , Araújo Castro, C. , Rolim Campos, A. and Vasconcellos Abdon, A. (2014) Effectiveness of Laser Therapy (AlGaInP) on Cicatrization of Experimental Cutaneous Wound in Rats. International Journal of Clinical Medicine, 5, 525-530. doi: 10.4236/ijcm.2014.59072.
References
[1]   Rhett, J.M., Ghatnekar, G.S., Palatinus, J.A., O’Quinn, M., Yost, M. J. and Reg, G. (2008) Novel Therapies for Scar Reduction and Regenerative Healing of Skin Wounds. Trends in Biotechnology, 26, 173-180.
http://dx.doi.org/10.1016/j.tibtech.2007.12.007

[2]   Cullum, N. (2001) Systematic Reviews of Wound Care Management: (5) Beds; (6) Compression; (7) Laser Therapy, Therapeutic Ultrasound, Electrotherapy and Electromagnetic Therapy. International Journal of Technology Assessment in Health Care, 5, 1-227.

[3]   Coelho, M.B., de Souza, I.A., Freire, M.G., Marangoni, S., Antunes, E. and Macedo, M.L. (2006) Neutrophil Migration in Mice Induced by a Mannose-Binding Lectin Isolated from Annona coriacea Seeds. Toxicon, 48, 529-535.
http://dx.doi.org/10.1016/j.toxicon.2006.07.001

[4]   Branski, R.C., Rosen, C.A., Verdolini, K. and Hebda, P.A. (2005) Biochemical Markers Associated with Acute Vocal fold Wound Healing: A Rabbit Model. Journal of Voice, 19, 283-289.
http://dx.doi.org/10.1016/j.jvoice.2004.04.003

[5]   Rocha Jr., A.M., Andrade, L.C.F., Oliveira, R.G., Aarestrup, F.M. and Farias, R.E. (2006) Modulation of Fibroblast Proliferation and Inflammatory Response by Low-Intensity Laser Therapy in Tissue Repair Process. Anais Brasileiros de Dermatologia, 81, 150-156.

[6]   Woodruff, L.D., Bounkeo, J.M., Brannon, W.M., Dawes, K.S., Barham, C.D. and Waddell, D.L. (2004) The Efficacy of Laser Therapy in Wound Repair: A Meta-Analysis of the Literature. Photomedicine and Laser Surgery, 22, 241-247.
http://dx.doi.org/10.1089/1549541041438623

[7]   Reis, S.R.A., Medrado, A.P., Marchionni, A.M.T., Figueira, C., Fracassi, L.D. and Knop, L.A.H. (2008) Effect of 670-nm Laser Therapy and Dexamethasone on Tissue Repair: A Histological and Ultrastructural Study. Photomedicine and Laser Surgery, 26, 307-313. http://dx.doi.org/10.1089/pho.2007.2151

[8]   Mavver-Biscanin, M., Mravak-Stipetic, M. and Jerolimov, V. (2005) Effects of Low-Level Laser Therapy on Candida Albicans Growth in Patients with Denture Stomatitis. Photomedicine and Laser Surgery, 23, 328-332.
http://dx.doi.org/10.1089/pho.2005.23.328

[9]   Pardi, G. and Cardozo, E.I. (2002) Detection de especies de candida en pacientes con stomatitis sub-protésica. Acta Odontológica Venezolana, 40, 9-17.

[10]   Wanessa, K.B., Giordano, C.G., Juliano, S.L., Fabiola, M.M., Silvana, M.S., Mariane, E. and Emerson, A.C. (2010) Use of ALGaInP Laser in the Wound Healing of Experimental Cutaneous Lesions in Rabbits. Acta Scientiae Veterinariae, 38, 237-243.

[11]   Prata, M.B., Haddad, C.M., Goldenberg, S, de J. Simöes, M., de Moura, L.A.R. and Trabulsi, L.R. (1988) Uso tópico do açúcar em ferida cutanea. Estudo experimental em rato. Acta Cirurgica Brasileira, 3, 43-48.

[12]   Ramsey, D.T., Papa, E.R., Mann, W.C., Berg, J.N. and Swaim, S.F. (1995) Effects of three occlusive dressing materials on healing of full-thickness skin wounds in dogs. American Journal of Veterinary Research, 56, 941-949.

[13]   Pugliese, L.S., Medrado, A.P., Reis, S.R. and de Araújo Andrade, Z. (2003) The Influence of Low-Level Laser Therapy on Biomodulation of Collagen and Elastic Fibers. Pesquisa Odontológica Brasileira, 17, 307-313.
http://dx.doi.org/10.1590/S1517-74912003000400003

[14]   Guirro, R.R.J. and Guirro, E.C.O. (2004) Analysis of the Transmissivity of Low Power Laser Radiation in Different Occlusive Dressings. 5th Congress of the World Association for Laser Therapy, Guarujá, 19.

[15]   Gál, P., Vidinsky, B., Toporcer, T., Mokry, M., Mozes, S. and Longauer, F. (2006) Histological Assessment of the Effect of Laser Irradiation on Skin Wound Healing in Rats. Photomedicine and Laser Surgery, 24, 480-488.
http://dx.doi.org/10.1089/pho.2006.24.480

[16]   Cressoni, M.D.C., Giusti, H.H.K.D. and Casaroto, R.A. (2008) The Effects of a 785-nm AIGaInP Laser on the Regeneration of Rat Anterior Tibialis Muscle after Surgically—Induced Injury. Photomedicine and Laser Surgery, 26, 461-466. http://dx.doi.org/10.1089/pho.2007.2150

[17]   Zhang, X.J., Chinkes, D.L., Cox, R.A. and Wolfe, R.R. (2006) The Flow Phase of Wound Metabolism Is Characterized by Stimulated Protein Synthesis Rather Than Cell Proliferation. Journal of Surgical Research, 135, 61-67.
http://dx.doi.org/10.1016/j.jss.2006.03.003

[18]   Iunes, D.H., Ferreira, L.R. and Reis, N.S. (2005) Comparative Study on the Use of Therapeutic Ltrasound and Heliumneon Laser (He-Ne) for Treating Experimental Wounds in Wistar Rats. Investigação, 5, 50-57.

[19]   Vincensi, C. and Carvalho, P.T.C. (2002) The Effects of the Laser Gallium Arsenide (AsGa) in Treatment of Inflammation in the Articulation in Wistar Rats through the Complete Adjuvant of Freund. Fisioterapia Brasil, 3, 223-230.

[20]   Medrado, A.R., Pugliese, L.S. and Reis, S.R. (2003) Influence of Low-Level Laser Therapy on Wound Healing and Its Biologicalaction upon Myofibroblasts. Lasers in Surgery and Medicine, 32, 239-244.
http://dx.doi.org/10.1002/lsm.10126

[21]   Mello, P.B., Sampedro, R.M.F. and Piccinini, A.M. (2007) Effects of the He Ne Laser and of the Application Mode on Healing Burns in Rats. Fisiotererapia & Pesquisa, 14, 6-13.

 
 
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