JCT  Vol.5 No.5 , April 2014
Immune Response Indicated by Expressing of IL-2 and IL-10 in Cervical Cancer
ABSTRACT

Introduction: Interleukin-2 (IL-2) is multiple which functions during an inflammatory response. Inflammation is a critical component of cancer progression. IL-10 is the most important cytokine with anti-inflammatory properties. Cancer patients typically show depression of both cellular and humoral immune functions. Aims: The objective of this experiment is to know the expression of IL-2 and IL-10 in cervical cancer. Method: Paraffin block of the tissues frozen section cervical cancer was cut in sharp and cleaned cryotome and place in glass plate that covered with poly-elysine. The immunohistochemistry stains were done with monoclonal antibody anti IL-2 and IL-10 with TSA-indirect method. The collected data were analyzed with T Test (SPSS for window 15). Result: In this study the expression of IL-2 (35.9% = moderate) is less than the expression of IL-10 (45.3% = moderate). From T Test analysis of p <= 0.153, it means that there is no significance in difference between IL-2 and IL-10. Conclusion: We indicated that the immune response plays a role in balance between cellular and humoral immunity.


Cite this paper
Prayitno, A. , Astirin, O. and Putra, S. (2014) Immune Response Indicated by Expressing of IL-2 and IL-10 in Cervical Cancer. Journal of Cancer Therapy, 5, 420-426. doi: 10.4236/jct.2014.55048.
References
[1]   Charles, A.D. and Lyle, L.M. (2000) Proinflammatory and Anti-Inflammatory Cytokines in Rheumatoid Arthritis. 2nd Edition, Amgen Inc., One Amgen Centre Drive, Thousand Oaks, 23-75.

[2]   Bufler, P. (2011) Interleukin-37 as Fundamental Inhibitor of Innate Immunity. Arthritis Research & Therapy, 13, 2011. http://dx.doi.org/10.1186/ar3419

[3]   Malek, T.R. (2003) The Main Function of IL-2 Is to Promote the Development of T Regulatory Cells. Journal of Leukocyte Biology, 74, 961-965. http://dx.doi.org/10.1189/jlb.0603272

[4]   Moore, K.W., O’Garra, A., de Waal Malefyt, R., Vieira, P. and Mosmann, T.R. (1993) Interleukin-10. The Annual Review of Immunology, 11, 165-190. http://dx.doi.org/10.1146/annurev.iy.11.040193.001121

[5]   Adi, P. (2006) Cervical Cancer with Human Papilloma Virus and Epstein Barr Virus Positive. Journal of Carcinogenesis, 5, 2006. http://dx.doi.org/10.1186/1477-3163-5-13

[6]   Chaturvedi, A.K. (2010) Beyond Cervical Cancer: Burden of Other HPV-Related Cancers among Men and Women. Journal of Adolescent Health, 46, 20-26. http://dx.doi.org/10.1016/j.jadohealth. 2010.01.016

[7]   Doll, R. and Peto, R. (1981) The Causes of Cancer: Quantitative Estimates of Avoidable Risks of Cancer in the United States Today. Journal of the National Cancer Institute, 66, 1191-1308.

[8]   Kinzler, Kenneth, W. and Vogelstein, B. (2002) Introduction. The Genetic Basis of Human Cancer. 2nd Edition, McGraw-Hill, New York, Medical Publications Division.

[9]   Khorasanizadeh, F., Hassanloo, J., Khaksar, N., Mohammad, T.S., Marzaban, M.H., Rasidi, B., Akbari, S.A., Zendehdel, K. (2013) Epidemiology of Cervical Cancer and Human Papilloma virus Infection among Iranian Women-Analyses of National Data and Systematic Review of the Literature. Gynecologic Oncology, 128, 277-281.

[10]   Licciarone, J.C., Wilkins, J.R., Brownson, R.C. and Chang, J.C. (1989) Cigarette Smoking and Alcohol Consumption in the Aetiology of Uterine Cervical Cancer. International Journal of Epidemiology, 18, 533-537. http://dx.doi.org/10.1093/ije/18.3.533

[11]   Elizabeth, A., Tara, N.R., Frannie, R.K., Chaterine, H. and Edward, S.P. (2009) Oral Cancer Knowledge, Attitude and Practices: A Survey of Dentist and Primary Care Physicians in Massachusetts. American Dental Association, 140, 461-467.

[12]   Colin, R.Y. and Welsh, C.J. (2005) Stress Health and Disease. Cell Science.

[13]   Edgar, H.V., Maria, E.C. and Paola (2007) A Solar dan Fabian A Soto. Enhancement of Pavlovian Conditioned Immunosuppression in Rats. Acta Neurobiologiae Experimentalis, 67, 71-81.

[14]   Prasanta, K.R. (1999) Stress Genes and Species Survival. Moleculer and Cellular Biochemistry. Springer Netherlands, 196, 117-123.

[15]   Putra, S.T. (2005) Psikoneuroimunologi. Graha Masyarakat Ilmiah Kedokteran (GRAMIK) Fakultas Kedokteran UNAIR-RSU dr Sutomo. Surabaya.

[16]   Elizabeth, C. (2007) Cell Stress Protein Linked to Breast Cancer.

[17]   Colin, R. (2005) Young dan C. Jane Welsh. Stress Health and Disease. Cell Science.

[18]   Alberto, J.L. and Macario, M.D. (2005) dan Everly Conway de Macario. Sick Chaperones, Cellular Stress, and Disease. NEJM, 353, 1489-1501. http://dx.doi.org/10.1056/NEJMra050111

[19]   Sunitha, C. and Gabriel, R. (2004) Oral Cancer at a Glance. The Internet Journal of Dental Science, 1, 2004.

[20]   Mehrotra Ravi dan Yadav, S. (2006) Oral Squamous Cell Carcinoma: Etiology, Pathogenesis and Prognostic Value of Genomic Alterations. Indian Journal of Cancer, 43, 60-66.
http://dx.doi.org/10.4103/0019-509X.25886

[21]   Coussens, L.M. and Werb, Z. (2002) Inflammation and Cancer. Nature, 420, 860-867.

[22]   Keibel, A., Singh, V. and Sharma, M.C. (2009) Inflammation, Microenvironment, and the Immune System in Cancer Progression. Current Pharmaceutical Design, 15, 1949-1955.
http://dx.doi.org/10.2174/138161209788453167

[23]   Weber, D., Wheat, J.M. and Curri, G.M. (2010) Inflammation and Cancer: Tumor Initiation, Progression and Metastasis, and Chinese Botanical Medicines. Journal of Chinese Integrative Medicine, 8, 1006-1013. http://dx.doi.org/10.3736/jcim20101101

[24]   Hoyer, K.K., Doom, H., Barron, L. and Abbas, A.K. (2008) Interleukin-2 in the Development and Control of Inflammatory Disease. Immunological Reviews, 226, 19-28.
http://dx.doi.org/10.1111/j.1600-065X.2008.00697.x

[25]   Botti, C., Seregni, E., Ferrari, L., Martinetti, A. and Bombardieri, E. (1998) Immunosuppressive Factors: Role in Cancer Development and Progression. International Journal of Biological Markers, 13, 51-69.

[26]   Beckebaum, S., Zhang, X., Chen, X., Yu, Z., Frilling, A., Dworacki, G., Grosse-Wilde, H., Broelsch, C.E., Gerken, G. and Cicinnati, V.R. (2004) Increased Levels of Interleukin-10 in Serum from Patients with Hepatocellular Carcinoma Correlate with Profound Numerical Deficiencies and Immature Phenotype of Circulating Dendritic Cell Subsets. Clinical Cancer Research, 10, 7260-7269.
http://dx.doi.org/10.1158/1078-0432.CCR-04-0872

[27]   Kim, R., Emi, M., Tanabe, K. and Arihiro, K. (2006) Tumor-Driven Evolution of Immunosuppressive Networks during Malignant Progression. Cancer Research, 66, 5527-5536.
http://dx.doi.org/10.1158/0008-5472.CAN-05-4128

[28]   IHC & ISH Advanced Staining (2013) Tutorial. An Animated Guide to Immunohistochemistry. http://www.LeicaBiosystem.com

[29]   Kim, S.H., Shin, Y.K., Lee, K.M., Lee, J.S., Yun, J.H. and Lee, S.M. (2003) An Improved Protocol of Biotinylated Tyramine-Based Immunohistochemistry Minimizing Nonspecific Background Staining. Journal of Histochemistry & Cytochemistry, 51, 129-132. http://dx.doi.org/10.1177/0022155403051 00116

[30]   Dinarello, C.A. (2000) Proinflammatory Cytokines. Chest, 118, 503-508.
http://dx.doi.org/10.1378/chest.118.2.503

[31]   Elenkov, I.J., Lezzoni, D.G., Daly, A., Harris, A.G. and Chrouros, G.P. (2005) Cytokine Dysregulation, Inflammation and Well-Being. Neuroimmunomodulation, 12, 255-269. http://dx.doi.org/10.1159/ 000087104

[32]   Bamias, G., Corridoni, D., Pizarro, T.T. and Cominelli, F. (2012) New Insights into the Dichotomous Role of Innate Cytokines in Gut Homeostasis and Inflammation. Cytokine, 59, 451-459.
http://dx.doi.org/10.1016/j.cyto.2012.06.014

[33]   Trinchieri, G., Kubin, M., Bellone, G. and Cassatella, M.A. (1993) Cytokine Cross-Talk between Phagocytic Cells and Lymphocytes: Relevance for Differentiation/Activation of Phagocytic Cells and Regulation of Adaptive Immunity. Journal of Cellular Biochemistry, 53, 301-308.
http://dx.doi.org/10.1002/jcb.240530406

[34]   Shen-Orr, S.S., Goldberger, O., Garten, Y., Rosenberg-Hasson, Y., Lovelace, P.A., Hirschberg, D.L., Altman, R.B., Davis, M.M. and Butte, A.J. (2009) Towards a Cytokine-Cell Interaction Knowledgebase of the Adaptive Immune System. Pacific Symposium on Biocomputing, 2009, 439-450.

[35]   Benedict, K.F. and Lauffenburger, D.A. (2013) Insights into Proteomic Immune Cell Signaling and Communication via Data-Driven Modeling. Current Topics in Microbiology and Immunology, 363, 201-233. http://dx.doi.org/10.1007/82_2012_249

[36]   Malek, T.R. (2008) The Biology of Interleukin-2. Annual Review of Immunology, 26, 453-479. http://dx.doi.org/10.1146/annurev.immunol.26.021607.090357

[37]   Kimura, H. and Yamaguchi, Y. (1989) Adoptive Immunotherapy with LAK Cell and IL-2 against Primary Lung Cancer. Nihon Rinsho Geka Gakkai Zasshi, 90, 1459-1462.

[38]   Wolk, K., Kunz, S., Asadullah, K. and Sabat, R. (2002) Cutting Edge: Immune Cells as Sources and Targets of the IL-10 Family Members? Journal of Immunology, 168, 5397-5402.

[39]   Itoh, K. and Hirohata, S. (1995) The Role of IL-10 in Human B Cell Activation, Proliferation, and Differentiation. Journal of Immunology, 154, 4341-4350.

[40]   Sabat, R., Grutz, G., Warszawska, K., Kirsch, S., Witte, E., Wolk, K. and Geginat, J. (2010) Biology of Interleukin-10. Cytokine & Growth Factor Reviews, 21, 331-344. http://dx.doi.org/10.1016/j.cytogfr. 2010.09.002

[41]   Bouaziz, J.D., Calbo, S., Maho-Vaillant, M., Saussine, A., Bagot, M., Bensussan, A. and Musette, P. (2010) IL-10 Produced by Activated Human B Cells Regulates CD4+ T-cell Activation in vitro. European Journal of Immunology, 40, 2686-2691. http://dx.doi.org/10.1002/eji.201040673

[42]   Lalani, I., Bhol, K. and Ahmed, A.R. (1997) Interleukin-10: Biology, Role in Inflammation and Autoimmunity. Annals of Allergy, Asthma & Immunology, 79, 469-484.
http://dx.doi.org/10.1016/S1081-1206(10)63052-9

[43]   Ouyang, W., Rutz, S., Crellin, N.K., Valdez, P.A. and Hymowitz, S.G. (2011) Regulation and Functions of the IL-10 Family of Cytokines in Inflammation and Disease. Annual Review of Immunology, 29, 71-109. http://dx.doi.org/10.1146/annurev-immunol-031210-101312

 
 
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