JWARP  Vol.6 No.5 , April 2014
New Records of Microcystins in Some Bulgarian Water Bodies of Health and Conservational Importance
ABSTRACT

Microcystins cause acute hepatotoxicity and chronic liver tumor promotion. This study presents the results of HPLC DAD analyses and their LC-MS confirmation of samples from five Bulgarian water bodies (reservoirs Stoudena, Pchelina, Bistritsa and lakes Dourankoulak, Vaya). The total concentration of microcystins in water samples ranged from 0.1 to 26.5 μg/l. The amount of microcystins in the biomasses ranged from 11.4 to 49.6 μg/g (d.w.). The high percent of positive samples in which the most toxic microcystin-LR is recorded, can serve as a strong alarm for the necessity of a serious study and relevant discussion of the problem with responsible authorities at national level.


Cite this paper
Pavlova, V. , Stoyneva, M. , Georgieva, V. , Donchev, D. , Spoof, L. , Meriluoto, J. , Bratanova, Z. and Karadjova, I. (2014) New Records of Microcystins in Some Bulgarian Water Bodies of Health and Conservational Importance. Journal of Water Resource and Protection, 6, 446-453. doi: 10.4236/jwarp.2014.65044.
References
[1]   Chorus, I. and Bartram, J. (1999) Toxic Cyanobacteria in Water: A Guide to Public Health Significance, Monitoring and Management. E&FN Spon, London. http://dx.doi.org/10.4324/9780203478073

[2]   WHO (1998) Guidelines for Drinking-Water Quality, Health Criteria and other Supporting Information. 2nd Edition, Addendum to 2, World Health Organization, Geneva.

[3]   Sivonen, K. and Jones, G.J. (1999) Cyanobacterial Toxins. In: Chorus, I. and Bartram, J., Eds., Toxic Cyanobacteria in Water: A Guide to Their Public Health Consequences, Monitoring and Management, E&FN Spon, London, 41-91.

[4]   Stoyneva, M.P. (1998) Development of the Phytoplankton of the Shallow Srebarna Lake (North-Eastern Bulgaria) Across the Trophic Gradient. In: Alvarez-Cobelas, M.C., Reynolds, S., Sanchez-Castillo, P. and Kristiansen, J., Eds., Phytoplankton and Trophic Gradients, Hydrobiologia, 369/370, 259-367.

[5]   Stoyneva, M.P. (2000) Algological Studies of Bulgarian Coastal Wetlands. I. Species Composition of the Phytoplankton of Durankulak and Shabla-Ezeretz Lakes. Ann. Univ. Sof., 91, 27-48.

[6]   Stoyneva, M.P. (2002) Algological Studies of Bulgarian Coastal Wetlands. II. Quantitative Structure of the Phytoplankton of Durankulak and Shabla-Ezeretz Lakes. Ann. Univ. Sof., 92, 91-109.

[7]   Stoyneva, M.P. (2003) Steady-State Phytoplankton Assemblages in Shallow Bulgarian Wetlands. In: Naselli-Flores, L., Padisak, J. and Dokulil, M., Eds., Phytoplankton and Equilibrium Concept: The Ecology of Steady-State Assemblages. Kluwer Academic Publishers, 169-176.

[8]   Pavlova, V., Stoyneva, M., Babica, P., Kohoutek, J. and Bratanova, Z. (2007) Microcystins Contamination and Cyanoprokaryote Bloomsin Some Coastal Bulgarian Wetlands. Conference Preprint Book, BULAQUA 2007, Second International Conference and Exhibition of Water Resources, Technologies and Services, Sofia, 221-226.

[9]   Stoyneva, M.P. and Michev, T.M. (2007) State-of-Art Survey of Bulgarian Non-Lotic Wetlands and their Biodiversity. In: Michev, T.M. and Stoyneva, M.P., Eds., Inventory of Bulgarian Wetlands and their Biodiversity. Part 1: Non-Lotic Wetlands. Publ. House Elsi-M, Sofia, 88-108.

[10]   Stoyneva, M.P. and Temniskova-Topalova, D.N. (2007) Cyanoprokaryotes of Bulgarian Non-Lotic Wetlands and their Biodiversity. In: Michev, T.M. and Stoyneva, M.P., Eds., Inventory of Bulgarian Wetlands and their Biodiversity. Part 1: Non-Lotic Wetlands. Publ. House Elsi-M, Sofia, 155-167.

[11]   Pavlova, V., Babica, P., Todorova, D., Bratanova, Z. and Marsalek, B. (2006) Contamination of Some Reservoirs and Lakes in Republic of Bulgaria by Microcystins. Acta Hydrochim Hydrobiol, 34, 437-441.

[12]   Pavlova, V. (2007) Hygiene and Analytical Aspects of Microcystins Occurrence in Surface Water. Ph.D. Dissertation, National Center of Public Health Protection, Sofia.

[13]   Teneva, I., Mladenov, R., Belkinova, D., Dimitrova-Dylgerova, I. and Dzhambazov, B. (2010) Phytoplankton Community of the Drinking Water Supply Reservoir Borovitsa (South Bulgaria) with an Emphasis on Cyanotoxins and Water Quality. Central European Journal of Biology, 5, 231-239.

[14]   Teneva, I., Belkinova, D., Dimitrova-Dylgerova, I. and Mladenov, R. (2009) Phytoplankton Assemblages and Monitoring of Cyanotoxins in Trakiets Reservoir. Scientific Researches of the Union of Scientists in Bulgaria-Plovdiv, Series B, Natural Sciences and the Humanities, 12, Technics, Technologies, Natural Sciences and Humanities Session.

[15]   Teneva, I., Belkinova, D., Dimitrova-Dylgerova, I., Vlaknova, M. and Mladenov, R (2010) Comparison and Toxic Potential of Cyanoprokaryota in Vacha Dam (Bulgaria). Second Balkan Conference on Biology, Plovdiv, 21-23 May 2010.

[16]   Michev, T.M. and Stoyneva, M.P. (2007) Inventory of Bulgarian Wetlands and Their Biodiversity. Part 1: Non-Lotic Wetlands. Publishing House Elsi-M, Sofia, 364.

[17]   Komarek, J. and Anagnostidis, K. (1999) Cyanoprokaryota. 1. Teil: Chroococcales. In: Ettl, H., Gartner, G., Heynig, G. and Mollenhauer, D., Eds., Sußwasserflora von Mitteleuropa, Bd., Gustav Fischer, Jena.

[18]   Komarek, J. and Anagnostidis, K. (2005) Cyanoprokaryota. 2. Teil: Oscillatoriales. In: Budel, B., Gartner, G., Krienitz, L. and Schagerl, M., Eds., Sußwasserflora von Mitteleuropa, Bd., Elsevier GmbH, Munchen.

[19]   Hindak, F. (2008) Colour Atlas of Cyanophytes. VEDA, Bratislava.

[20]   Temniskova, D. and Stoyneva, M. (2011) Algology. General Part. Vol. 1. Pensoft, Sofia-Moscow, 1-512 [In Bulgarian].

[21]   Temniskova, D. and Stoyneva, M. (2011) Algology. Systematic Part. Vol. 2. Pensoft, Sofia-Moscow, 513-1040 [In Bulgarian].

[22]   Rott, E. (1981) Some Results from Phytoplankton Counting Intercalibration. Schweizerische Zeitschrift fur Hydrologie, 43, 34-62.

[23]   Deisinger, G. (1984) Leitfaden zur Bestimmung der planktischen Algen der Karntner Seen und ihrer Biomasse Zusammengestellt von. Karntner Institut fur Seenforschung.

[24]   Poon, G.K., Griggs, L.J., Edwards, C., Beattie, K.A. and Codd, G.A. (1993) Liquid Chromatography-Electrospray Ionization-Mass Spectrometry of Cyanobacterial Toxins. Journal of Chromatography A, 628, 215-233.
http://dx.doi.org/10.1016/0021-9673(93)80005-S

[25]   Wu, Z., Shi, J., Xiao, P., Liu, Y. and Li, R. (2011) Phylogenetic Analysis of Two Cyanobacterial Genera Cylindrospermopsis and Raphidiopsis Based on Multi-Gene Sequences. Harmful Algae, 10, 419-425.
http://dx.doi.org/10.1016/j.hal.2010.05.001

[26]   Li, R., Carmichael, W.W., Brittain, S., Eaglesham, G.K., Shaw, G.R., Liu, Y. and Watanabe, M. (2001) First Report on the Cyanotoxins Cylindrospermopsin and Deoxycylindrospermopsin from Raphidiopsis curvata (Cyanobacteria). Journal of Phycology, 37, 1121-1126. http://dx.doi.org/10.1046/j.1529-8817.2001.01075.x

[27]   Namikoshi, M., Murakami, T., Watanabe, M.F., Oda, T., Yamada, J., Tsujimura, S., Nagai, H. and Oishi, S. (2003) Simultaneous Production of Homoanatoxin-a, Anatoxin-a, and a New Non-Toxic 4-Hydroxyhomoanatoxin-a by the Cyanobacterium Raphidiopsis mediterranea Skuja. Toxicon, 42, 533-538.

[28]   Stucken, K., John, U., Cembella, A., Murillo, A.A., Soto-Liebe, K., Fuentes-Valdes, J.J., Friedel, M., Plominsky, A.M., Vasquez, M. and Glockneret, G. (2010) The Smallest Known Genomes of Multicellular and Toxic Cyanobacteria: Comparison, Minimal Gene Sets for Linked Traits and the Evolutionary Implications. PLoS ONE, 5, Article ID: e9235.
http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0009235

[29]   Jiang, Y., Xiao, P., Yu, G., Sano, T., Pan, Q. and Lia, R. (2012) Molecular Basis and Phylogenetic Implications of Deoxycylindrospermopsin Biosynthesis in the Cyanobacterium Raphidiopsis curvata. Applied and Environmental Microbiology, 78, 2256-2263.

[30]   Stoyneva, M., Traykov, I., Uzunov, B., Tosheva, A. and Zidarova, R. (2013) Final Report of Ecotan EOOD on the Monitoring of Bulgarian Lakes on the Basis of the WFD Biological Element “Phytoplankton” for the Period 2011-2013 for the Executive Agency of Environment at the Ministry of Environment and Waters of Bulgaria.

[31]   Michev, T.M. and Stoyneva, M.P. (2005) Red List of Bulgarian Wetlands: Conception, Creation and Application. Annuaire de l’Université de Sofia “St KlimentOhridski”, Livre 4-10ème Session Scientifigue, Sofia’03, Partie II, Tome 96, 71-76.

[32]   Michev, T.M. and Stoyneva, M.P. (2007) Conservation of Bulgarian Non-lotic Wetlands. In: Michev, T.M. and Stoyneva, M.P., Eds, Inventory of Bulgarian Wetlands and Their Biodiversity, Part 1: Non-Lotic Wetlands, Publishing House Elsi-M, Sofia, 109-131.

[33]   Kondratieva, N.V. (1968) Questions of Morphology and Systematics of Microcystisaeruginosa Kutz. emend. Elenk. and Its Close Species. In: Water Blooms, Vol. 1, 13-42 [In Russian].

[34]   Kondratieva, N.V. and Kovalenko, O.N. (1975) Short Quide to the Species of Toxic Blue-Green Algae. Publishing House Naukova doumka, Kiiv, 80 [In Russian].

[35]   Kirpenko, Y.A., Sirenko, L.A., Orlovskii, V.M. and Lukina L.F. (1977) Blue-Green Algal Toxins and the Animal Organism. Publishing House Naukovadoumka, Kiiv, 251 [In Russian].

[36]   Dow, C.S. and Swoboda, U.K. (2000) Cyanotoxins. In: Whitton, B.A. and Potts, M., Eds., The Ecology of Cyanobacteria, Their Diversity in Time and Space, Kluwer Academic Publishers, Dordrecht, London, Boston, 613-632.

[37]   Xu, Y., Wu, Z.X., Yu, B.S., Peng, X., Yu, G.L., Wei, Z.H., Wang, G.X. and Li, R.H. (2008) Non-Microcystin Producing Microcystis wesenbergii (Komarek) Komarek (Cyanobacteria) Representing a Main Waterbloom-Forming Species in Chinese Waters. Environmental Pollution, 156, 162-167. http://dx.doi.org/10.1016/j.envpol.2007.12.027

[38]   Vaitomaa, J., Rapala, J., Seitsonen, J. and Sivonen, K. (1998) Factors Influencing Growth and Toxin Production of Cyanobacteria: Field, Mesocosm and Laboratory Experiments. In: Carmichael, W.W. and Paerl, H.W., Eds, Abstracts, 4th International Conference on Toxic Cyanobacteria, Beaufort, 51.

[39]   Dimitrova, I. and Hainadjieva, V. (2006) Water Pollution in the Durankulak Lake, Bulgaria. In: Balwois 2006 Conference, 23-26 May 2006, Ohrid, Republic of Macedonia.
http://www.balwois.com/balwois/administration/full_paper/ffp-694.pdf

[40]   Albay, M., Matthiensen, A. and Codd, G. (2005) Occurrence of Toxic Blue-Green Algae in the Kucukcekmece Lagoon (Istanbul, Turkey). Environmental Toxicology, 20, 277-284.

[41]   Funari, E., Gramaccioni, L. and Scardala, S. (2005) Cyanobacteria in Surface Water. In: Chorus, I., Ed., Current Approaches to Cyanotoxin Risk Assessment, Risk Management and Regulations in Different Countries, WaBoLu-Hefte, Federal Environmental Agency (Umweltbundesamt), Berlin, 81-83.

[42]   Mankiewicz, J., Jurczak, T., Izydorczyk, K. and Zalewski, M. (2005) Regulation on Cyanotoxins in Legislation. In: Chorus, I., Ed., Current Approaches to Cyanotoxin Risk Assessment, Risk Management and Regulations in Different Countries, Wa BoLu-Hefte. Federal Environmental Agency (Umweltbundesamt), Berlin, 99-101.

[43]   Pantelic, D., Svircev, Z., Simeonovic, J., Vidovic, V. and Trajkovic, I. (2013) Cyanotoxins: Characteristics, Production and Degradation Routes in Drinking Water Treatment with Reference to the Situation in Serbia. Chemosphere, 1, 421-441.

[44]   Blinkova, M., Zdraveski, N. and Krastic, S.S. (2012) Blue-Green Algae, the Janus of Nature. In: Proceedings of the 5th International Scientific Conference BALWOIS 2012, 28 May-2 June 2012, Ohrid.

[45]   Cook, C.M., Moustaka-Gouni, M., Gkelis, S. and Lanaras, T. (2005) Cyanotoxin Risk Assessment, Risk Management and Regulation. In: Chorus, I., Ed., Current Approaches to Cyanotoxin Risk Assessment, Risk Management and Regulations in Different Countries, WaBoLu-Hefte, Federal Environmental Agency (Umweltbundesamt), Berlin, 69-75.

[46]   Boaru, D.A., Dragos, N., Welker, M., Bauer, A., Nicoara, A. and Schirmer, K. (2006) Toxic Potential of Microcystin-Containing Cyanobacterial Extracts from Three Romanian Freshwaters. Toxicon, 47, 925-932.

[47]   Jukovic, M., Svircev, Z., Baltic, V., Stojanovic, D., Mihajlovic, A. and Baltic, M. (2008) Cyanotoxins-New Health Risk Factor in Serbia. Archive of Oncology, 16, 55-58.

[48]   Blahova, L., Babica, P., Adamovsky, O., Kohoutek, J., Marsalek, B. and Blaha, L. (2008) Analyses of Cyanobacterial Toxins (Microcystins, Cylindrospermopsin) in the Reservoirs of the Czech Republic and Evaluation of Health Risks. Environmental Chemistry Letters, 6, 223-227. http://dx.doi.org/10.1007/s10311-007-0126-x

[49]   Marsalek, B., Blaha, L. and Hindak, F. (2000) Review of Toxicity of Cyanobacteria in Slovakia. Biologia, 55, 645-652.

[50]   Spoof, L., Vesterkvist, P., Lindholm, T. and Meriluoto, J. (2003) Screening for Cyanobacerial Hepatotoxins, Microcyatins and Nodularin in Environmental Water Samples by Reversed-Phase Liquid Chromatography-Electrospray Ionization Mass Spectrometry. Journal of Chromatogaphy A, 1020, 105-119.
http://dx.doi.org/10.1016/S0021-9673(03)00428-X

[51]   WHO (2003) Guidelines for Safe Recreational Water Enviroments. Vol. 1. Coastal and Fresh Water. Chapter 8. Algae and Cyanobacteria in Fresh water. World Health Organization, Geneva, 151.

 
 
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