Antibacterial Activity of Polyethylenimine/Carrageenan Multilayer against Pathogenic Bacteria
Affiliation(s)
Industrial Technology Development Institute, DOST Complex, Taguig, Philippines. Department of Biosciences and Informatics, Keio University, Yokohama, Japan.
Industrial Technology Development Institute, DOST Complex, Taguig, Philippines. Department of Biosciences and Informatics, Keio University, Yokohama, Japan.
ABSTRACT
The multilayer of polyethylenimine (PEI) and carrageenan (k, i, l) formed by layer-by-layer assembly was investigated for its antibacterial activity against Enterobacter cloaceae, Staphylococcus aureus and Enterococcus faecalis 29505 for potential use as coating on biomaterial surface. All the multilayers exhibited growth inhibition. PEI/Iota carrageenan multilayer was effective in inhibiting the growth of the E. cloaceae, S. aureus and E. faecalis while PEI/Lambda carrageenan was effective in inhibiting the growth of E. cloaceae. Results of the paper strip test for combined action of carrageenan and PEI showed synergism with regards to bacterial growth inhibition. The multilayers had also contact-killing effect with the test organisms. The multilayer was also characterized by atomic force microscopy (AFM), X-ray photoelectron spectroscopy (XPS) and biomolecular interaction analysis.
Cite this paper
Briones, A. , Sato, T. and Bigol, U. (2014) Antibacterial Activity of Polyethylenimine/Carrageenan Multilayer against Pathogenic Bacteria. Advances in Chemical Engineering and Science, 4, 233-241. doi: 10.4236/aces.2014.42026.
Briones, A. , Sato, T. and Bigol, U. (2014) Antibacterial Activity of Polyethylenimine/Carrageenan Multilayer against Pathogenic Bacteria. Advances in Chemical Engineering and Science, 4, 233-241. doi: 10.4236/aces.2014.42026.
References
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http://dx.doi.org/10.1002/app.12055 [23] Decher, G. (1997) Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites. Science, 277, 1232-1237.
http://dx.doi.org/10.1126/science.277.5330.1232 [24] Decher, G., Hong, J.D. and Schmitt, J. (1992) Buildup of Ultrathin Multilayer Films by A Self-Assembly Process. III. Consecutively Alternating Adsorption of Anionic and Cationic Polyelectrolytes on Charged Surfaces. Thin Solid Films, 210-211, 831-835. http://dx.doi.org/10.1016/0040-6090(92)90417-A [25] Rodrigues, S., Rosa da Costa, A.M. and Grenha, A. (2012) Chitosan/Carrageenan Nanoparticles: Effect of Cross-Linking with Tripolyphosphate and Charge Ratios. Carbohydrate Polymers, 89, 282-289.
http://dx.doi.org/10.1016/j.carbpol.2012.03.010
[1] Mendelson, J.D., Yang, S.Y., Hiller, J., Hochbaum, A.I. and Rubner, M.F. (2003) Rational Design of Cytophilic and Cytophobic Polyelectrolyte Multilayer Thin Films. Biomacromolecules, 4, 96-106.
http://dx.doi.org/10.1021/bm0256101 [2] Elbert, D.L., Herbert, C.B. and Hubbell, J.A. (1999) Thin Polymer Layers Formed by Polyelectrolyte Multilayer Techniques on Biological Surfaces. Langmuir, 15, 5355-5362.
http://dx.doi.org/10.1021/la9815749 [3] Richert, L., Lavalle, P., Payan, E., Shu, X., Prestwich, G., Stoltz, J., Schaaf, P., Voegel, J. and Picart, C. (2004) Layer by Layer Buildup of Polysaccharide Films: Physical Chemistry and Cellular Adhesion Aspects. Langmuir, 20, 448-458.
http://dx.doi.org/10.1021/la035415n [4] Sharma, S., Johnson, R.W. and Desai, T.A. (2004) Evaluation of the Stability of Nonfouling Ultrathin Poly(ethylene glycol) Films for Silicon-Based Micro Devices. Langmuir, 20, 348-356.
http://dx.doi.org/10.1021/la034753l [5] Roosjen, A., Van der Mei, H.C., Busscher, H.J. and Norde, W. (2004) Microbial Adhesion to Poly(ethylene oxide) Brushes: Influence of Polymer Chain Length and Temperature. Langmuir, 20, 10949-55.
http://dx.doi.org/10.1021/la048469l [6] Yamato, M., Konno, C., Utsumi, M., Kikuchi, A. and Okano, T. (2002) Thermally Responsive Polymer-Grafted Surfaces Facilitate Patterned Cell Seeding and Co-Culture. Biomaterials, 23, 561-567.
http://dx.doi.org/10.1016/S0142-9612(01)00138-7 [7] Hu, S.G., Jou, C.H. and Yang, M.C. (2002) Surface Grafting of Polyester Fiber. Journal of Applied Polymer Science, 86, 2977-2983. http://dx.doi.org/10.1002/app.11261 [8] Cen, L., Neoh, K.G. and Kang, E.T. (2003) Surface Functionalization Techniques for Conferring Anti Bacterial Properties to Polymeric and Cellulosic Surfaces. Langmuir, 19, 10295-10303.
http://dx.doi.org/10.1021/la035104c [9] Kanazawa, A., Ikeda, T. and Endo, T. (1994) Polymeric Phosphonium Salts as a Novel Class of Cationic Biocides. VI. Antibacterial Activity of Fibers Surface-Treated with Phosphonium Salts Containing Trimethoxysilane Groups. Journal of Applied Polymer Science, 52, 641-647.
http://dx.doi.org/10.1002/app.1994.070520507 [10] Gristina, A.G. (1987) Biomaterial-Centered Infection: Microbial Adhesion versus Tissue Integration. Science, 237, 1588-1595. http://dx.doi.org/10.1126/science.3629258 [11] Bratskaya, S., Marinin, D., Simon, F., Synytska, A., Zschoche, S., Busscher, H.J., Jager, D. and van der Mei, H.C. (2007) Adhesion and Viability of Two Enterococcal Strains on Covalently Grafted Chitosan and Chitosan/k-Carrageenan Multilayers. Biomacro Molecules, 8, 2960-2968.
http://dx.doi.org/10.1021/bm700620j [12] Khalil, H., Chen, T., Riffon, R., Wang, R. and Wang, Z. (2008) Synergy between Polyethylenimine and Different Families of Antibiotics against a Resistant Clinical Isolate of Pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy, 52, 1635-1641. http://dx.doi.org/10.1128/AAC.01071-07 [13] Helander, I.M., Alakomi, H., Latva-Kala, K. and Koski, P. (1997) Polyethylenimine Is an Effective Permeabilizer of Gram-Negative Bacteria. Microbiology, 143, 3193-3199.
http://dx.doi.org/10.1099/00221287-143-10-3193 [14] Shvero, D.K., Davidi, M.P., Weiss, E.I., Srerer, N. and Beyth, N. (2010) Antibacterial Effect of Polyethyleneimine Nanoparticles Incorporated in Provisional Cements against Streptococcus mutans. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 94, 367-371.
http://dx.doi.org/10.1002/jbm.b.31662 [15] Beyth, N., Houri-Haddad, Y., Baraness-Hadar, L., Yudovin-Farber, I., Domb, A.J. and Weiss, E.I. (2008) Surface Antimicrobial Activity and Biocompatibility of Incorporated Polyethylenimine Nanoparticles. Biomaterials, 29, 4157-4163. http://dx.doi.org/10.1016/j.biomaterials.2008.07.003 [16] (2007) Evaluation of Certain Food Additives and Contaminants. Sixty-Eighth Report of the Joint FAO/WHO Expert Committee on Food Additives. WHO Technical Report Series 947, 32. [17] Richardson, R.K. and Goycoolea, F.M. (1994) Rheological Measurement of k-Carrageenan during Gelation. Carbohydrate Polymer, 24, 223-225. http://dx.doi.org/10.1016/0144-8617(94)90134-1 [18] Schoeler, B., Delorme, N., Doench, I., Sukhorukov, G.B., Fery, A. and Glinel, K. (2003) Polyelectrolyte Films Based on Polysaccharides of Different Confrmations: Effects on Multilayer Structure and Mechanical Properties. Biomacromolecules, 7, 2065-2071. http://dx.doi.org/10.1021/bm060378a [19] Lee, S.S., Lee, K.B. and Hong, J.D. (2003) Evidence for Spin Coating Electrostatic Self-Assembly of Polyelectrolytes. Langmuir, 19, 7592-7596. http://dx.doi.org/10.1021/la034263t [20] Wang, F.F., Yao, Z., Wu, H.G., Zhang, S.X., Zhu, N.N. and Gai, X. (2011) Antibacterial Activities of Kappa-Carrageenan Oligosaccharides. Applied Mechanics and Materials, 108, 194-199. [21] Briones, A.V. and Sato, T. (2011) Formation of Polyethylenimine/Carrageenan Bi-Layer as Monitored by Atomic Force Microscopy and Biomolecular Interaction Analysis. International Proceedings of Chemical, Biological and Environmental Engineering Journal, 24, 288-291. http://dx.doi.org/10.7763/ipcbee [22] Hu, S.G., Jou, C.H. and Yang, M.C. (2002) Antibacterial and Biodegradable Properties of Polyhydroxyalkanoates Grafted with Chitosan and Chitooligosaccharides via Ozone Treatment. Journal of Applied Polymer Science, 86, 2977-2983.
http://dx.doi.org/10.1002/app.12055 [23] Decher, G. (1997) Fuzzy Nanoassemblies: Toward Layered Polymeric Multicomposites. Science, 277, 1232-1237.
http://dx.doi.org/10.1126/science.277.5330.1232 [24] Decher, G., Hong, J.D. and Schmitt, J. (1992) Buildup of Ultrathin Multilayer Films by A Self-Assembly Process. III. Consecutively Alternating Adsorption of Anionic and Cationic Polyelectrolytes on Charged Surfaces. Thin Solid Films, 210-211, 831-835. http://dx.doi.org/10.1016/0040-6090(92)90417-A [25] Rodrigues, S., Rosa da Costa, A.M. and Grenha, A. (2012) Chitosan/Carrageenan Nanoparticles: Effect of Cross-Linking with Tripolyphosphate and Charge Ratios. Carbohydrate Polymers, 89, 282-289.
http://dx.doi.org/10.1016/j.carbpol.2012.03.010