AiM  Vol.4 No.5 , April 2014
Identification and Expression Analysis of Regulatory Genes Induced by Near-Ultraviolet Irradiation in Bipolaris oryzae
ABSTRACT

Bipolaris oryzae is the causal agent of brown leaf spot disease in rice, and its asexual spore (conidium) formation is known to be induced by near-ultraviolet (NUV) irradiation. In order to reveal the photomorphogenic response and to identify new genes upregulated by NUV irradiation, suppression subtractive hybridization (SSH) was carried out in B. oryzae. To confirm the differential gene expression in NUV-irradiated mycelia, quantitative real-time PCR (qRT-PCR) analysis was performed among 301 genes arbitrarily chosen from 1170 cDNA clones. The expression of 46 genes (named NUV01 to NUV46) was found to be significantly enhanced (>4-fold) by NUV irradiation. Sequence analysis revealed that 23 out of the 46 sequences (50%) showed significant matches to known fungal genes. The 46 genes were categorized as either BLR1-dependent or BLR1-independent expression groups using the BLR1-deficient mutant, which presumably lacks the blue/UVA-absorbing photoreceptor. This finding demonstrates that NUV irradiation can induce gene regulation, and that this response may be mediated by both a blue/UVA-absorbing photoreceptor and an as-yet-unidentified photoreceptor in B. oryzae.


Cite this paper
Kihara, J. , Tanaka, N. , Ueno, M. and Arase, S. (2014) Identification and Expression Analysis of Regulatory Genes Induced by Near-Ultraviolet Irradiation in Bipolaris oryzae. Advances in Microbiology, 4, 233-241. doi: 10.4236/aim.2014.45030.
References
[1]   Herrera-Estrella, A. and Horwitz, B.A. (2006) Looking through the Eyes of Fungi: Molecular Genetics of Photoreception. Molecular Microbiology, 64, 5-15. http://dx.doi.org/10.1111/j.1365-2958.2007.05632.x

[2]   Corrochano, L.M. (2007) Fungal Photoreceptors: Sensory Molecules for Fungal Development and Behaviour. Photochemical & Photobiological Sciences, 6, 725-736. http://dx.doi.org/10.1039/b702155k

[3]   Moony, J.J. and Yager, L.N. (1990) Light Is Required for Conidiationin Aspergillus nidulans. Genes & Development, 4, 1473-1482. http://dx.doi.org/10.1101/gad.4.9.1473

[4]   Leach, C.M. (1961) The Sporulation of Helminthosporium oryzae as Affected by Exposure to Near Ultraviolet Radiation and Dark Periods. Canadian Journal of Botany, 39, 705-715. http://dx.doi.org/10.1139/b61-057

[5]   Kumagai, T. (1983) Action Spectra for the Blue and Near-Ultraviolet Reversible Photoreaction in the Induction of Fungal Conidiation. Physiologia Plantarum, 57, 468-471. http://dx.doi.org/10.1111/j.1399-3054.1983.tb02770.x

[6]   Purschwits, J., Müller, S., Kastner, C. and Fischer, R. (2006) Seeing the Rainbow: Light Sensing in Fungi. Current Opinion in Microbiology, 9, 566-571. http://dx.doi.org/10.1016/j.mib.2006.10.011

[7]   Rodriguez-Romero, J., Hedtke, M., Kastner, C., Müller, S. and Fischer, R. (2010) Fungi, Hidden in Soil or Up in the Air: Light Makes a Difference. Annual Review of Microbiology, 64, 585-610.
http://dx.doi.org/10.1146/annurev.micro.112408.134000

[8]   Kihara, J., Sato, A., Okajima, S. and Kumagai, T. (2001) Molecular Cloning, Sequence Analysis and Expression of Novel Gene Induced by Near-UV Light in Bipolaris oryzae. Molecular Genetics and Genomics, 266, 64-71.
http://dx.doi.org/10.1007/s004380100519

[9]   Kihara, J., Moriwaki, A., Ito, M., Arase, S. and Honda, Y. (2004) Expression of THR1, a 1,3,8-THN Reductase Gene Involved in Melanin Biosynthesis in the Phytopathogenic Fungus Bipolaris oryzae, Is Enhanced by Near-Ultraviolet Radiation. Pigment Cell Research, 17, 15-23. http://dx.doi.org/10.1046/j.1600-0749.2003.00102.x

[10]   Kihara, J., Moriwaki, A., Ueno, M., Tokunaga, T., Arase, S. and Honda, Y. (2004) Cloning, Functional Analysis and Expression of a Scytalonedehydratase Gene (SCD1) Involved in Melanin Biosynthesis of the Phytopathogenic Fungus Bipolaris oryzae. Current Genetics, 45, 197-204. http://dx.doi.org/10.1007/s00294-003-0477-1

[11]   Moriwaki, A., Kihara, J., Kobayashi, T., Tokunaga, T., Arase, S. and Honda, Y. (2004) Insertional Mutagenesis and Characterization of a Polyketide Synthase Gene (PKS1) Required for Melanin Biosynthesis in Bipolaris oryzae. FEMS Microbiology Letters, 238, 1-8. http://dx.doi.org/10.1111/j.1574-6968.2004.tb09729.x

[12]   Kihara, J., Moriwaki, A., Tanaka, N., Tanaka, C., Ueno, M. and Arase, S. (2008) Characterization of the BMR1 Gene Encoding a Transcriptional Factor for Melanin Biosynthesis Genes in the Phytopathogenic Fungus Bipolaris oryzae. FEMS Microbiology Letters, 281, 221-227. http://dx.doi.org/10.1111/j.1574-6968.2008.01101.x

[13]   Kihara, J., Moriwaki, A., Matsuo, N., Arase, S. and Honda, Y. (2004) Cloning, Functional Characterization, and Near-Ultraviolet Radiation-Enhanced Expression of a Photolyase Gene (PHR1) from the Phytopathogenic Fungus Bipolaris oryzae. Current Genetics, 46, 37-46. http://dx.doi.org/10.1007/s00294-004-0507-7

[14]   Berlin, V. and Yanofsky, C. (1985) Isolation and Characterization of Genes Differentially Expressed during Conidiation of Neurospora crassa. Molecular and Cellular Biology, 5, 849-855. http://mcb.asm.org/content/5/4/849

[15]   Rosales-Saavedra, T., Esquivel-Naranjo, E.U., Casas-Flores, S., Martínez-Hernández, P., Ibarra-Laclette, E., Cortes-Penagos, C. and Herrera-Estrella, A. (2006) Novel Light-Regulated Genes in Trichodera atroviride: A Dissection by cDNA Microarrays. Microbiology, 152, 3305-3317. http://dx.doi.org/10.1099/mic.0.29000-0

[16]   Lu, J.P., Liu, T.B. and Lin, F.C. (2005) Identification of Mature Appressorium-Enriched Transcripts in Magnaporthe grisea, the Rice Blast Fungus, Using Suppression Subtractive Hybridization. FEMS Microbiology Letters, 245, 131-137. http://dx.doi.org/10.1016/j.femsle.2005.02.032

[17]   Flaherty, J.E. and Dunkle, L.D. (2005) Identification and Expression Analysis of Regulatory Genes Induced during Conidiation in Exserohilum turcicum. Fungal Genetics and Biology, 42, 471-481.
http://dx.doi.org/10.1016/j.fgb.2005.02.001

[18]   Verwoerd, T.C., Dekker, B.M.M. and Hoekema, A. (1989) A Small-Scale Procedure for the Rapid Isolation of Plant RNAs. Nucleic Acid Research, 17, 2362. http://dx.doi.org/10.1093/nar/17.6.2362

[19]   Kihara, J., Tanaka, N., Ueno, M. and Arase, S. (2009) Cloning and Expression Analysis of Two Opsin-Like Genes in the Phytopathogenic Fungus Bipolaris oryzae. FEMS Microbiology Letters, 295, 289-294.
http://dx.doi.org/10.1111/j.1574-6968.2009.01609.x

[20]   Kihara, J., Moriwaki, A., Tanaka, N., Ueno, M. and Arase, S. (2007) Characterization of the BLR1 Gene Encoding a Putative Blue-Light Regulator in the Phytopathogenic Fungus Bipolarisoryzae. FEMS Microbiology Letters, 266, 110-118. http://dx.doi.org/10.1111/j.1574-6968.2006.00514.x

[21]   Linden, H., Ballario, P. and Macino, G. (1997) Blue Light Regulation in Neurosporacrassa. Fungal Genetics and Biology, 22, 141-150. http://dx.doi.org/10.1006/fgbi.1997.1013

[22]   Chen, C.H., Dunlap, J.C. and Loros, J.J. (2010) Neurospora Illuminates Fungal Photoreception. Fungal Genetics and Biology, 47, 922-929. http://dx.doi.org/10.1016/j.fgb.2010.07.005

[23]   Bayram, O., Braus, G.H., Fischer, R. and Rodriguez-Romero, J. (2010) Spotlight on Aspergillus nidulans Photosensory Systems. Fungal Genetics and Biology, 47, 900-908. http://dx.doi.org/10.1016/j.fgb.2010.05.008

[24]   Kliebenstein, D.J., Lim, J.E., Landry, L.G. and Last, R.L. (2002) Arabidopsis UVR8 Regulates Ultraviolet-B Signaling Transduction and Tolerance and Contains Sequence Similarity to Human Regulator of Chromatin Condensation 1. Plant Physiology, 130, 234-243. http://dx.doi.org/10.1104/pp.005041

[25]   Heijde, M. and Ulm, R. (2012) UV-B Photoreceptor-Mediated Signaling in Plants. Trends in Plant Science, 17, 230-237. http://dx.doi.org/10.1016/j.tplants.2012.01.007

[26]   Idnurm, A., Verma, S. and Corrochano, L.M. (2010) A Glimpse into the Basis of Vision in the Kingdom Mycota. Fungal Genetics and Biology, 47, 881-892. http://dx.doi.org/10.1016/j.fgb.2010.04.009

[27]   Moriwaki, A., Katsube, H., Ueno, M., Arase, S. and Kihara, J. (2008) Cloning and Characterization of the BLR2, the Homologue of the Blue-Light Regulator of Neurospora crassa WC-2, in the Phytopathogen in Fungus Bipolaris oryzae. Current Microbiology, l56, 115-121. http://dx.doi.org/10.1007/s00284-007-9080-x

 
 
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