In order to assess chicken T cell-mediated responses after immune stress, 200 two-week-old chickens were randomly divided into control group(C) and treatment groups (T1 and T2). The live I-type of Newcastle disease vaccine (ND) was taken as the source of immunological stress. The chickens in group (T2) were injected with overdose of live I-type Newcastle disease vaccine. After vaccination, the dynamic changes of CD4+, CD8+T cells on thymus were detected by immunohisto-chemistry. The ultra-structure of T cells on thymus of the chickens in group (T2) was observed by electron microscopic. The result showed: (1) After immune stress, from day 1 to day 5, the number of the CD4+T cells significantly declined and reached the bottom at day 5, but the number of the CD8+T cells increased dramatically and peaked at day 5. (2) After immune stress, from day 1 to day 5, part of T cells of chicken thymus came with apoptotic and pathological changes of putrescence continually. These results underscore: the immune stress can cause transient immune adjustment. These changes for chickens were the self-protection mechanism of immune system to adapt to survival and avoid immune disorder.
Cite this paper
Jia, Z. , Cao, Y. , Xue, Y. , Li, F. , Liu, M. , Zhang, C. , Yang, Y. and Duan, J. (2014) Analysis of Chicken T Cell-Mediated Responses on Thymus after Immune Stress. Journal of Immune Based Therapies, Vaccines and Antimicrobials
, 22-28. doi: 10.4236/jibtva.2014.32003
 Dalgaard, T.S., et al. (2010) Flow Cytometric Assessment of Chicken T Cell-Mediated Immune Responses after Newcastle Disease Virus Vaccination and Challenge. Vaccine, 28, 4506-4514.http://dx.doi.org/10.1016/j.vaccine.2010.04.044
 Dominguez-Gerpe, L. and Rey-Mendez, M. (2000) Role of Pre-T Cells and Chemoattractants on Stress-Associated Thymus Involution. Scandinavian Journal of Immunology, 52, 470-476.
 Mast, J., et al. (2006) Vaccination of Chicken Embryos with Escape Mutants of La Sota Newcastle Disease Virus Induces a Protective Immune Response. Vaccine, 24, 1756-1765. http://dx.doi.org/10.1016/j.vaccine.2005.10.020
 Knueppel, D., et al. (2010) Impact of Vaccination against Chicken Newcastle Disease on Food Intake and Food Security in Rural Households in Tanzania. Food and Nutrition Bulletin, 1, 31, 436-445.
 Mansoori, B. and Modirsanei, M. (2012) Effects of Dietary Tannic Acid and Vaccination on the Course of Coccidiosis in Experimentally Challenged Broiler Chicken. Veterinary Parasitology, 187, 119-122.http://dx.doi.org/10.1016/j.vetpar.2011.12.016
 Gao, W.W and Ren, J.Y. (2001) The Influence of Experimental Immunological Stress on Immunological Function of Chicken. Journal of Shanxi Agricultural Sciences, 29, 80-84.
 Tian, W.X. (2000) Effect of Dynamic Changes of Antibody Producing Cells of Immune Organs and Tissues of Experemental Immunologic Stress Chickens. Journal of Shanxi Agricultural University, 3, 232-235.
 Cattoretti, G., et al. (1988) Improved Avidin-Biotin-Peroxidase Complex (ABC) Staining. Histochemical Journal, 20, 75-80.http://dx.doi.org/10.1007/BF01746607
 Henning, J., et al. (2009) Evaluation of Strategies to Improve Village Chicken Production: Controlled Field Trials to Assess Effects of Newcastle Disease Vaccination and Altered Chick Rearing in Myanmar [Corrected]. Preventive Veterinary Medicine, 90, 17-30. http://dx.doi.org/10.1016/j.prevetmed.2009.04.007
 Xu, T., Gao, W. and Ren, J.Y. (2002) Effects of Experimental Immunologic Stress on Blood Hormone and Immunity in Chickens. Acta Agriculturae Boreali-Sinica, 17, 114-118.
 Lv, Q., Zhang, S. and Zhao, R. (2008) Transportation Stress Alters the Expression of Immunoregulatory Cytokines in the Porcine Thymus. The Veterinary Journal, 187, 229-233. http://dx.doi.org/10.1016/j.tvjl.2009.12.004
 Frazer, I.H. and Mackay, I.R. (1985) A Graphical Presentation of Counts of T Lymphocyte Subpopulations and Th-Ts Ratios. Pathology, 17, 62-63.http://dx.doi.org/10.3109/00313028509063726
 Franchini, A., Marchesini, E. and Ottaviani, E. (2004) Corticosterone 21-Acetate in Vivo Induces Acute Stress in Chicken Thymus: Cell Proliferation, Apoptosis and Cytokine Responses. Histology and Histopathology, 19, 693-699.
 Dhabhar, F.S. and McEwen, B.S. (1999) Enhancing versus Suppressive Effects of Stress Hormones on Skin Immune Function. Proceedings of the National Academy of Sciences of the USA, 96, 1059-1064.http://dx.doi.org/10.1073/pnas.96.3.1059