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 AJPS  Vol.5 No.9 , April 2014
Characterization of Congolese Strains of Xanthomonas axonopodis pv. manihotis Associated with Cassava Bacterial Blight
Abstract: Cassava bacterial blight (CBB) caused by Xanthomonas axonopodis pv. manihotis has been reported in several African countries since 1970. Knowledge of the virulence and diversity of Xanthomonas axonopodis pv. manihotis strains is important for an integrated control of CBB. The main objective of the present study was to characterize strains of Xanthomonas axonopodis collected from various regions in the DR-Congo. There was variability among strains for shape (form), contour (margin) and elevation. Bacterial cell size for the strains analyzed varied from 24.1 μm × 11.3 μm to 11.4 μm × 4.2 μm. All the Xanthomonas axonopodis pv. manihotis strains but one was motile. Two distinctive groups were identified based on radial growth of their colonies. The first group grows faster (7.8-10.5 mm/d) compared to the second group (4.8-6.9 mm/d). Five strains (Gandajika, Inera/Stat, Kansasa, Mulumba and Musakatshi) were classified as virulent with a damage rating ≤ 1 and four were aggressive (Luputa, M'vuazi, Boketa and Kiyaka) with a damage rating > 1. Significant differences were also observed among strains for disease onset, incidence and plant mortality. The highest incidence (33%) of bacterial blight 21 days after infestation (DAI) resulted from the Boketa strain inoculation and the lowest (0 % disease incidence) from INERA/STAT and Musakatshi strains. There was no clear association between geographic origin of the strains and their aggressiveness.
Cite this paper: Mamba-Mbayi, G. , Tshilenge-Djim, P. , Nkongolo, K. and Kalonji-Mbuyi, A. (2014) Characterization of Congolese Strains of Xanthomonas axonopodis pv. manihotis Associated with Cassava Bacterial Blight. American Journal of Plant Sciences, 5, 1191-1201. doi: 10.4236/ajps.2014.59132.
References

[1]   Lozano, J.C. and Sequeira, L. (1974) Bacterial Blight of Cassava in Colombia—Epidemiology and Control. Phytopathology, 64, 83-88. http://dx.doi.org/10.1094/Phyto-64-83

[2]   Maraite, H. and Meyer, J.A. (1975) Xanthomonas manihotis (Arthaud-Berthet) Starr, Causal Agent of Bacterial Wilt, Blight and Leaf Spots of Cassava in Zaire. PANS Pest Articles & News Summaries, 21, 27-37.

[3]   Lozano, J.C. (1986) Cassava Bacterial-Blight—A Manageable Disease. Plant Disease, 70, 1089-1093.
http://dx.doi.org/10.1094/PD-70-1089

[4]   Nilmanee, S. (1986) Diseases of Cassava. In: Raychaudhuri, S.P. and Verma, J.P., Eds., Resistance Breeding in Root and Tuber Crops at International Institute of Tropical Agriculture (IITA), Ibadan, Nigeria, Today & Tomorrow’s Printers & Publishers, New Delhi, 213-247.

[5]   Maraite, H., Weyns, J., Yimkwan, O., Lipembra, P. and Perreaux, D. (1982) Physiological and Pathogenic Variations in Xanthomonas axonopodis pv. manihotis. In: J. C. Lozano and P. H. Gwin, Eds., Proceedings of the Fifth International Conference on Plant Pathogenic Bacteria, Cali, 16-23 August 1981, 358-368.

[6]   Alves, M.L.B. and Takatsu, A. (1984) Variabilidade em Xanthomonas campestris pv. manihotis. Fitopatologia Brasiliera, 9, 485-494.

[7]   Ogunjobi, A.A., Fagade, O.E. and Dixon, A.G.O. (2010) Physiological Studies on Xanthomonas axonopodis pv manihotis (Xam) Strains Isolated in Nigeria. European Journal of Biological Sciences, 2, 84-90.

[8]   Restrepo, S. and Verdier, V. (1997) Geographical Differentiation of the Population of Xanthomonas axonopodis pv. manihotis in Colombia. Applied and Environmental Microbiology, 63, 4427-4434.

[9]   Verdier, V., Boher, B., Maraite, H. and Geiger, J. (1994) Pathological and Molecular Characterization of Xanthomonas campestris Strains Causing Diseases of Cassava (Manihot esculenta). Applied and Environmental Microbiology, 60, 4478-4486.

[10]   Jorge, V. and Verdier, V. (2002) Qualitative and Quantitative Evaluation of Cassava Bacterial Blight Resistance in F1 Progeny of a Cross between Elite Cassava Clones. Euphytica, 123, 41-48. http://dx.doi.org/10.1023/A:1014400823817

[11]   Boher, B. and Verdier, V. (1994) Cassava Bacterial Blight in Africa: The State of Knowledge and Implications to Designing Control Strategies. African crop science journal, 2, 1.

[12]   Restrepo, S., Duque, M.C. and Verdier, V. (2000) Resistance Spectrum of Selected Manihot esculenta Genotypes under Field Conditions. Field Crops Research, 65, 69-77. http://dx.doi.org/10.1016/S0378-4290(99)00072-6

[13]   Restrepo, S., Duque, M.C. and Verdier, V. (2000) Characterization of pathotypes among isolates of Xanthomonas axonopodis pv. manihotis in Colombia. Plant Pathology, 49, 680-687.
http://dx.doi.org/10.1046/j.1365-3059.2000.00513.x

[14]   Banito, A. (2003) Integrated Control of Cassava Bacterial Blight in West Africa in Relation to Ecozones, Host Plant Resistance and Cultural Practices. http://edok01.tib.uni-hannover.de/edoks/e01dh03/36701954X.pdf

[15]   Olutiola, P.O., Famurewa, O. and Sonntag, H.G. (1991) An introduction to General Microbiology, A Practical Approach. Heidelberger Verlagsanstalt und Druckerei GmbH, Heidelberg, 267.

[16]   Ogunjobi, A.A., Fagade, O.E. and Dixon, A.G.O. (2008) Physiological Studies on Xanthomonas axonopodis Pv Manihotis (Xam) Strains Isolated in Nigeria. Advances in Biological Research, 2, 90-96.

[17]   Bradbury, J.F. (1979) Identification et Caractéristiques de Xanthomonas manihotis. In: Terry, E.R., Persley, G.J. and Cook, S.C.A., Eds., La Bactériose du Manioc en Afrique: Le Passé, le Présent, L'avenir: Compte Rendu du Séminaire Interdisciplinaire Qui S'est Tenu à L'IITA, Ibadan, Nigéria, du 26 Au 30 Juin 1978, Parrainé Par le Centre de Recherche Pour le Developpement International, Ottawa, Canada, et L'Institut International D'agriculture Tropicale, Ibadan, Nigeria, Natural Resources Institute, 1-4.

[18]   Fahy, P.C., Fahy, P.C. and Persley, G.J. (1983) Plant Bacterial Diseases: A Diagnostic Guide. Academic Press, Waltham.

[19]   Aygan, A. and Arikan, B. (2007) An Overview on Bacterial Motility Detection. International Journal of Agriculture and Biology, 9, 193-196.

[20]   Duveiller, E., Bragard, C., Rudolph, K. and Fucikovsky, L. (1997) General Concepts and Methods for the Identification of Pathogenic Bacteria of Wheat. In: Duveiller, E., Fucikovsky, L. and Rudolph, K., Eds., The Bacterial Diseases of Wheat: Concepts and Methods of Disease Management, CIMMYT, Mexico, 5-23.

[21]   Sanchez, G., Restrepo, S., Duque, M.C., Fregene, M., Bonierbale, M. and Verdier, V. (1999) AFLP Assessment of Genetic Variability in Cassava Accessions (Manihot esculenta) Resistant and Susceptible to the Cassava Bacterial Blight (CBB). Genome/National Research Council Canada, 42, 163-172.

[22]   Bansal, V.K., Kharbanda, P.D., Stringam, G.R., Thiagarajah, M.R. and Tewari, J.P. (1994) A Comparison of Greenhouse and Field Screening Methods for Blackleg Resistance in Doubled Haploid Lines of Brassica-Napus. Plant Disease, 78, 276-281. http://dx.doi.org/10.1094/PD-78-0276

[23]   Shaner, G. and Finney, R.E. (1977) Effect of Nitrogen-Fertilization on Expression of Slow-Mildewing Resistance in Knox Wheat. Phytopathology, 67, 1051-1056. http://dx.doi.org/10.1094/Phyto-67-1051

[24]   McGuire, R.G. and Jones, J.B. (1984) Evaluation of a Selective Medium for the Isolation of Xanthomonas campestris pv vesicatoria from Seeds and Foliage. Phytopathology, 74, 882-882.

[25]   McGuire, R.G. andJones, J.B. (1987) Recovery of Xanthomonas campestris pv. vesicatoria from Tomato Seed. Plant In: Pathogenic Bacteria, Current Plant Science and Biotechnology in Agriculture, Springer, 768.

[26]   Willems, A., Gillis, M., Kersters, K., Van Den Broecke, L. and De Ley, J. (1987) Transfer of Xanthomonas ampelina Panagopoulos 1969 to a New Genus, Xylophilus gen. nov., as Xylophilus ampelinus (Panagopoulos 1969) comb. nov. International Journal of Systematic Bacteriology, 37, 422-430.

[27]   Rodriguez, S., Stapleton, J.J. and Civerolo, E.L. (1987) Xanthomonas campestris Involved in Mexican Lime Bacteriosis in Colima, Mexico. In: Pathogenic Bacteria, Current Plant Science and Biotechnology in Agriculture, Springer, 658-662.

[28]   Takatsu, A., Fukuda, S. and Perrin, S. (1978) Epidemiological Aspects of Bacterial Blight of Cassava in Brazil. Proceedings of the International Symposium on Diseases of Tropical Food Crops, Belgium, 4-8 September 1978, 141-150.

[29]   Grousson, F., Pages, J. and Boher, B. (1990) Study of Variability of a Pathogenic Agent Xanthomonas campestris pv manihotis, by Multiple Factorial Analysis. Agronomie, 10, 627-640.
http://dx.doi.org/10.1051/agro:19900803

[30]   Fessehaie, A. (1997) Biochemical/physiological characterization and detection methods of Xanthomonas axonopodis pv. manihotis (Berthet-Bondar) Dye 1978, the causal organism of cassava bacterial blight. In: Anonymous Eds., University of Göttingen, Germany.

[31]   Wydra, K., Fessehaie, A., Fanou, A., Sikirou, R., Janse, J., Verdier, V. and Rudolph, K. (1998) Variability of isolates of Xanthomonas axonopodis pv. manihotis, incitant of cassava (Manihot esculenta Crantz) bacterial blight, from different geographic origins in pathological, biochemical and serological characteristics. In: Balasubramanian, R., Lalithakumari, D. and Mahadevan, A., Eds., Proceedings of the nineth international congress of Plant Pathogenic Bacteria 1996, University of Madras, Madras, 317-323.

[32]   Boher, B. and Agbobli, C.A. (1992) La bactériose vasculaire du manioc au Togo: Caractérisation du parasite, répartition géographique et sensibilité variétale. Agronomie Tropicale, 46, 131-136.

 
 
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