Enzyme Biosensor for Detection of Organophosphate Pesticide Residues Base on Screen Printed Carbon Electrode (SPCE)-Bovine Serum Albumin (BSA)

Ani  Mulyasuryani; Mochammad  Dofir

doi:10.4236/eng.2014.65027

Ani Mulyasuryani, Mochammad Dofir

Abstract: The maximum level of organophosphate pesticide residues in rice is 0.1 mg/kg and 0.5 mg/kg in vegetables. The control of pesticide residues in agricultural products required a method of analysis quickly and accurately. The research developed a biosensor for the detection of organophosphate pesticide residues in agricultural products. The research studied immobilized organophosphate hydrolase (OPH) mass and characterization of biosensor. The solution conductivity measurement in the conductivity cell consists of a 1 × 5 mm2 pair of electrodes screen printed carbon electrode (SPCE). The instrument is a converted local conductometer. From the results of study concluded that the optimum performance of the biosensor was obtained from the 105 μg OPH, at pH 8.5 with a response time of 45 seconds. In that condition the sensitivity of biosensor is 28.04 μS/ppm and 0.18 ppm detection limit and the maximum concentration of pesticide which can be measured is 1 ppm. Biosensors have been applied to measure pesticide residues in some vegetable samples.

Keywords: Conductometric Biosensor; Organophosphate Pesticides; Organophosphate Hydrolase; Screen Printed Carbon Electrode

Cite this paper: Mulyasuryani, A. and Dofir, M. (2014) Enzyme Biosensor for Detection of Organophosphate Pesticide Residues Base on Screen Printed Carbon Electrode (SPCE)-Bovine Serum Albumin (BSA). Engineering, 6, 230-235. doi: 10.4236/eng.2014.65027.

References

[1] Alegantina, S., Raini, M. and Lastari, P. (2005) Penelitian Kandungan Organofosfat Dalam Tomat Dan Slada Yang Beredar Di Beberapa Jenis Pasar Di DKI. Ministry of Health Republic of Indonesia, Jakarta.

[2] Ministry of Health and Agriculture Ministry Republic of Indonesia (1996) Batas Maksimum Residu Pestisida Pada Hasil Pertanian, Jakarta.

[3] Schulze, H., Scherbaum, E., Anastassiades, M., Vorlova, S., Schmiddan R.D. and Bachmann, T.T. (2002) Development, Validation, and Application of An Acetylcholinesterase-Biosensor Test for the Direct Detection of Insecticide Residues in Infant Food. University of Stuttgart, Stuttgart.

[4] Garcia, M.N.V. and Mottram, T. (2003) Biosensor Technology Addressing Agricultural Problems. Biosystems Engineering, 84, 1-12.
http://dx.doi.org/10.1016/S1537-5110(02)00236-2

[5] Pohanka, M., Jun, D. and Kuca, K. (2008) Amperometric Biosensors for Real Time Assays of Organophosphates. Sensors, 8, 5303-5312.
http://dx.doi.org/10.3390/s8095303

[6] Lee, J.H., Park, J.Y., Min, K., Cha, H.J., Choi, S.S. and Yoo, Y.J. (2010) A Novel Organophosphorus Hydrolase-Based Biosensor Using Mesoporous Carbons and Carbon Black for the Detection of Organophosphate Nerve Agents. Biosensors and Bioelectronics, 25, 1566-1570.
http://dx.doi.org/10.1016/j.bios.2009.10.013

[7] Chouteau, C., Dzyadevych, S., Chovelon, J.M. and Durrieu, C. (2004) Development of Novel Conductometric Biosensors Based on Immobilised Whole Cell Chlorella Vulgaris Microalgae. Biosensors and Bioelectronics, 19, 10891096.
http://dx.doi.org/10.1016/j.bios.2003.10.012

[8] Jaffrezic-Renault, N. and Dzyadevych, S.V. (2008) Conductometric Microbiosensors for Environmental Monitoring, Sensors, 8, 2569-2588.
http://dx.doi.org/10.3390/s8042569

[9] Gan, N., Yang, X., Xie, D., Wu, Y. and Wen, W. (2010) A Disposable Organophosphorus Pesticides Enzyme Biosensor Based on Magnetic Composite Nano-Particles Modified Screen Printed Carbon Electrode. Sensors, 10, 625-638.
http://dx.doi.org/10.3390/s100100625

[10] Mulyasuryani, A. and Srihardyastutie, A. (2011) Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode. Analytical Chemistry Insight, 6, 47-51.
http://dx.doi.org/10.4137/ACI.S7346

[11] Jaffrezic-Renault, N. (2001) New Trends in Biosensors for Organophosphorus Pesticides. Sensors, 1, 60-74.
http://dx.doi.org/10.3390/s10100060

[12] Egins, B.R. (2002) Chemical Sensors and Biosensors. John Wiley & Sons, LTD, Singapore.

[13] Wang, J. (2001) Analytical Electrochemistry. 2nd Edition, VCH Publisher, Inc., New York, 133-160.