In the present study, a
co-culture technique was adopted with an aim to investigate a hyper production of exoglucanase, and β-glucosidase
using cheap and easily available agro-industrial residue corn stover as growth
supporting substrate. Various physio-chemical and nutritional variables were
optimized using classical and completely randomized designs for induced
production of exoglucanase, and β-glucosidase
from the co-culture of Trichoderma viride and Ganoderma lucidum in solid state
fermentation (SSF). Analysis profile showed that when the conditions of the SSF
medium containing 15 g corn stover substrate (50% w/w moisture) inoculated with
6 mL of inoculum were optimal, the maximum productions of exoglucanase (485 ±
6.5 U/mL) and β-glucosidase (255 ±
3.3 U/mL) were recorded after 5 days of incubation at pH 6 and 35°C.
Cite this paper
Shahzadi, T. , Anwar, Z. , Iqbal, Z. , Anjum, A. , Aqil, T. ,  , B. , Afzal, A. , Kamran, M. , Mehmood, S. and Irshad, M. (2014) Induced Production of Exoglucanase, and β
-Glucosidase from Fungal Co-Culture of T. viride
and G. lucidum
. Advances in Bioscience and Biotechnology
, 426-433. doi: 10.4236/abb.2014.55051
 Iqbal, H.M.N., Asgher, M., Ahmed, I. and Hussain, S. (2010) Media Optimization for Hyper-Production of Carboxymethyl Cellulase Using Proximally Analyzed Agro-Industrial Residue with Trichoderma harzianum under SSF. IJAVMS, 4, 47-55.
 Irshad, M., Anwar, Z. and Afroz, A. (2012) Characterization of Exo 1, 4-β Glucanase Produced from Tricoderma viridi through Solid-State Bio-Processing of Orange Peel Waste. Advances in Bioscience and Biotechnology, 3, 580-584.http://dx.doi.org/10.4236/abb.2012.35075
 Iqbal, H.M.N., Ahmed, I., Zia, M.A. and Irfan, M. (2011) Purification and Characterization of the Kinetic Parameters of Cellulase Produced from Wheat Straw by Trichoderma viride under SSF and Its Detergent Compatibility. Advances in Bioscience and Biotechnology, 2, 149-156. http://dx.doi.org/10.4236/abb.2011.23024
 Iqbal, H.M.N., Asgher, M. and Bhatti, H.N. (2011) Optimization of Physical and Nutritional Factors for Synthesis of Lignin Degrading Enzymes by a Novel Strain of Trametes versicolor. BioResources, 6, 1273-87.
 Irshad, M., Anwar, Z., But, H.I., Afroz, A., Ikram, N. and Rashid, U. (2013) The Industrial Applicability of Purified Cellulase Complex Indigenously Produced by Trichoderma viride through Solid-State Bio-Processing of Agro-Industrial and Municipal Paper Wastes. BioResources, 8, 145-157.
 Iqbal, H.M.N., Kyazze, G. and Keshavarz, T. (2013) Advances in Valorization of Lignocellulosic Materials by Bio-Technology: An Overview. BioResources, 8, 3157-3176.
 Quiroz-Castañeda, R.E., Balcázar-López, E., Dantán-González, E., Martinez, A., Folch-Mallol, J. and Anaya, C.M. (2009) Characterization of Cellulolytic Activities of Bjerkandera adusta and Pycnoporus sanguineus on Solid Wheat Straw Medium. Electronic Journal of Biotechnology, 12, 5-6.
 Iqbal, H.M.N., Kamal, S., Ahmed, I. and Naveed, M.T. (2012) Enhanced Bio-Catalytic and Tolerance Properties of an Indigenous Cellulase through Xerogel Immobilization. Advances in Bioscience and Biotechnology, 3, 308-313. http://dx.doi.org/10.4236/abb.2012.34044
 Yano, S., Ozaki, H., Matsuo, S., Ito, M., Wakayama, M. and Takagi, K. (2012) Production, Purification and Characterization of D-Aspartate Oxidase from the Fungus Trichoderma harzianum SKW-36. Advances in Bioscience and Biotechnology, 3, 7-13. http://dx.doi.org/10.4236/abb.2012.31002
 Deshpande, M.V., Eriksson, K.E. and Göran Pettersson, L. (1984) An Assay for Selective Determination of Exo-1, 4,-β-Glucanases in a Mixture of Cellulolytic Enzymes. Analytical Biochemistry, 138, 481-487.http://dx.doi.org/10.1016/0003-2697(84)90843-1
 Gielkens, M.M.C., Dekkers, E., Visser, J. and De-Graaff, L.H. (1999) Two Cellobiohydrolase-Encoding Genes from Aspergillus niger Require D-Xylose and the Xylanolytic Transcriptional Activator XlnR for Their Expression. Applied and Environmental Microbiology, 65, 4340-4545.
 Chandra, M., Kalra, A. and Sharma, P.K. (2010) Optimization of Cellulases Production by Trichoderma citrinoviride on Marc of Artemisia annua and Its Application for Bioconversion Process. Biomass and Bioenergy, 34, 805-811. http://dx.doi.org/10.1016/j.biombioe.2010.01.024
 Pushalkar, S., Rao, K.K. and Menon, K. (1995) Production of β-Glucosidase by Aspergillus terrus. Current Microbiology, 30, 255-258. http://dx.doi.org/10.1007/BF00295497
 Omojasola, P.F. and Jilani, O.P. (2009) Cellulase Production by Trichoderma longi, Aspergillus niger and Saccharomyces cerevisae Cultured on Plantain Peel. Research in Microbiology, 4, 67-74. http://dx.doi.org/10.3923/jm.2009.67.74
 Mikiashvili, N., Elisashvili, V., Wasser, S. and Nevo, E. (2005) Carbon and Nitrogen Sources Influence the Ligninolytic Enzyme Activity of Trametes versicolor. Biotechnology Letters, 27, 955-959. http://dx.doi.org/10.1007/s10529-005-7662-x