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 AS  Vol.5 No.4 , March 2014
Nervous System and Gastrointestinal Effects of the Insecticide Esfenvalerate on the Rat: An Ex Vivo Study
Abstract: Esfenvalerate belongs to the pyrethroid group of insecticides which display significant selective toxicity against insects compared to mammalian species, nevertheless, they may pose health risks, especially in case of accidental exposure. The aim of the present study was to model the effect of acute, relatively high-dose exposure of the esfenvalerate-containing formulation Sumi-Alpha?. Eventual functional alterations in the central nervous system and in the gastrointestinal tract were studied on in vitro tissue preparations at different delays after intragastric administration to rats. Neuronal effects were characterized by field potential recording in cortical and hippocampal brain slices, while gastrointestinal effects were examined by analyzing the motility and excitability of isolated ileum segments. On the brain slices originating from esfenvalerate-treated animals, changes in excitability of both inhibitory and excitatory type could be observed. Voltage thresholds necessary to evoke responses in neocortex slices were elevated, and population spike amplitudes were lower in hippocampal slices. However, epileptiform potentials with pronounced late components were also observed. A decreased long-term potentiation (LTP) could be seen in both brain areas after esfenvalerate treatment. Seizure susceptibility of the slices was not significantly altered, but tended to be somewhat higher in slices originating from treated rats. In ileum segments, both spontaneous and acetyl-choline (ACh)-elicited contractions were modified by treatment. Esfenvalerate raised the amplitude of contractions in the low ACh concentration range. However, the solvent xylene also considerably contributed to the detected changes. We can conclude that a relatively high, single oral dose of Sumi-Alpha? exerted mild and temporary effects on the elementary brain functions and intestine functions of the rat.
Cite this paper: Varró, P. , Szabó, E. , Kovács, M. and Világi, I. (2014) Nervous System and Gastrointestinal Effects of the Insecticide Esfenvalerate on the Rat: An Ex Vivo Study. Agricultural Sciences, 5, 365-375. doi: 10.4236/as.2014.54038.
References

[1]   Casida, J.E. (1980) Pyrethrum Flowers and Pyrethroid Insecticides. Environmental Health Perspectives, 34, 189-202.
http://dx.doi.org/10.1289/ehp.8034189

[2]   Narahashi, T. (1992) Nerve Membrane Na+ Channels as Targets of Insecticides. Trends in Pharmacological Sciences, 13, 236-241. http://dx.doi.org/10.1016/0165-6147(92)90075-H

[3]   Catterall, W.A. (2000) From Ionic Currents to Molecular Mechanisms: The Structure and Function of Voltage-Gated Sodium Channels. Neuron, 26, 13-25. http://dx.doi.org/10.1016/S0896-6273(00)81133-2

[4]   Marban, E., Yamagishi, T. and Tomaselli, G.F. (1998) Structure and Function of Voltage-Gated Sodium Channels. The Journal of Physiology, 508, 647-657. http://dx.doi.org/10.1111/j.1469-7793.1998.647bp.x

[5]   Vais, H., Williamson, M.S., Devonshire, A.L. and Usherwood, P.N. (2001) The Molecular Interactions of Pyrethroid Insecticides with Insect and Mammalian Sodium Channels. Pest Management Science, 57, 877-888.
http://dx.doi.org/10.1002/ps.392

[6]   Song, J.H. and Narahashi, T. (1996) Modulation of Sodium Channels of Rat Cerebellar Purkinje Neurons by the Pyrethroid Tetramethrin. Journal of Pharmacology and Experimental Therapeutics, 277, 445-453.

[7]   Isobe, N., Kaneko, H., Shiba, K., Saito, K., Ito, S., Kakuta, N., Saito, A., Yoshitake, A. and Miyamoto, J. (1990) Metabolism of Esfenvalerate in Rats and Mice and Effects of Its Isomers on Metabolic Fates of Esfenvalerate. Journal of Pesticide Science, 15, 159-168. http://dx.doi.org/10.1584/jpestics.15.159

[8]   Parker, C.M., Albert, J.R., Vangelder, G.A., Patterson, D.R. and Taylor, J.L. (1985) Neuropharmacologic and Neuropathologic Effect of Fenvalerate in Mice and Rats. Fundamental and Applied Toxicology, 5, 278-286.
http://dx.doi.org/10.1016/0272-0590(85)90075-2

[9]   Furness, J.B. (2000) Types of Neurons in the Enteric Nervous System. Journal of the Autonomic Nervous System, 81, 87-96. http://dx.doi.org/10.1016/S0165-1838(00)00127-2

[10]   Galligan, J.J. (2002) Pharmacology of Synaptic Transmission in the Enteric Nervous System. Current Opinion in Pharmacology, 2, 623-629. http://dx.doi.org/10.1016/S1471-4892(02)00212-6

[11]   Varró, P., Szigyártó, I.Cs., Gergely, A., Kálmán, E. and Világi, I. (2013) Carbon Nanotubes Exert Basic Excitatory Enhancement in Rat Brain Slices. Acta Biologica Hungarica, 64, 137-151.
http://dx.doi.org/10.1016/S1471-4892(02)00212-6

[12]   Breckenridge, C.B., Holden, L., Sturgess, N., Weiner, M., Sheets, L., Sargent, D., Soderlund, D.M., Choi, J.S., Symington, S., Clark, J.M., Burr, S. and Ray, D. (2009) Evidence for a Separate Mechanism of Toxicity for the Type I and the Type II Pyrethroid Insecticides. Neurotoxicology, 30, S17-S31. http://dx.doi.org/10.1016/j.neuro.2009.09.002

[13]   Tabarean, I.V. and Narahashi, T. (1998) Potent Modulation of Tetrodotoxin-Sensitive and Tetrodotoxin-Resistant Sodium Channels by the Type II Pyrethroid Deltamethrin. Journal of Pharmacology and Experimental Therapeutics, 284, 958-965.

[14]   Wolansky, M.J. and Harrill, J.A. (2008) Neurobehavioral Toxicology of Pyrethroid Insecticides in Adult Animals: A Critical Review. Neurotoxicology and Teratology, 30, 55-78. http://dx.doi.org/10.1016/j.ntt.2007.10.005

[15]   Zhang, Y., Wang, Q., Wang, Z., Zhang, Q. and Zhang, Y. (2008) Neurobehavior and Learning and Memory of Mice after Postnatal Exposure to Fenvalerate. Journal of Toxicology, 2, 005.

[16]   Gao, B., Liu, Z. and Liu, X. (2009) Effect of Fenvalerate on the Activity of Acetylcholinesterase and Learning and Memory Function in Mouse Brain Tissue. Occupation and Health, 19, 005.

[17]   Vijverberg, H.P. and van den Bercken, J. (1990) Neurotoxicological Effects and the Mode of Action of Pyrethroid Insecticides. Critical Reviews in Toxicology, 21, 105-126. http://dx.doi.org/10.3109/10408449009089875

[18]   Williamson, E.G., Long, S.F., Kallman, M.J. and Wilson, M.C. (1989) A Comparative Analysis of the Acute Toxicity of Technical-Grade Pyrethroid Insecticides and Their Commercial Formulations. Ecotoxicology and Environmental safety, 18, 27-34. http://dx.doi.org/10.1016/0147-6513(89)90089-4

[19]   Wang, X.L., Jin, X.P., Fu, H., Chen, Z.Q., Da, C.D., Huang, X.F. and Ding, B.Q. (1999) Xylene-Induced Effects on Brain Neurotransmitters, Behavior and Fos Protein in Rats. Biomedical and Environmental Sciences, 12, 116-124.

[20]   Yang, P.Y., Lin, J.L., Hall, A.H., Tsao, T.C. and Chern, M.S. (2002) Acute Ingestion Poisoning with Insecticide Formulations Containing the Pyrethroid Permethrin, Xylene, and Surfactant: A Review of 48 Cases. Journal of Toxicology. Clinical Toxicology, 40, 107-113. http://dx.doi.org/10.1081/CLT-120004397

[21]   Neuschl, J., Kacmar, P. and Poracova, J. (1995) Toxicologic Evaluation of Supermethrin, a Pyrethroid Insecticide, in Rabbits and Pheasants. Veterinarni Medicina, 40, 383-386.

[22]   Tonini, M., Costa, L.G., Candura, S.M., Olibet, G., Rizzi, C.A., Garlaschelli, L. and Manzo, L. (1989) Interaction of the Pyrethroid Insecticides Tetramethrin and Cypermethrin with Enteric Cholinergic Transmission in the Guinea-Pig. Neurotoxicology, 10, 707-715.

[23]   Stankovic, J., Varagic, V. and Milovanovic, S. (2005) Modulatory Effects of Neurotoxic Insecticides on the Peripheral and Central GABA-Ergic Actions. Acta Veterinaria, 55, 193-201. http://dx.doi.org/10.2298/AVB0503193S

 
 
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