AJPS  Vol.5 No.8 , March 2014
Monitoring the Short-Term Response to Salt Exposure of Two Genetically Distinct Phragmites australis Clones with Different Salinity Tolerance Levels
Abstract: Aims: Two genetically distinct clones of Phragmites australis were used to investigate the immediate response induced by osmotic stress. The study aimed at elucidating if the response time, the inhibition rate and the recovery from salinity stress vary between these two genotypes. The experimental work was conducted at the laboratory of the Institute of Bioscience, Aarhus University, Denmark. Methods: The light-saturated photosynthetic rate (Pmax), stomata conductance (gs) and transpiration rate (E) were measured over different periods of salt exposure (15, 70 and 240 minutes) and at different salt concentrations (20 and 40 parts per thousand salinity). Important findings: The osmotic stress induced stomata closure and reduction of Pmax and E for both clones. The clone-specific responses as measured through physiological parameters were negatively correlated with exposure time and salt concentration. During the 4-hour exposure at 20 ppt, the two clones were inhibited at different rates. The salt-sensitive Land-type showed an immediate reduction of Pmax, gs and E. No recovery was observed after removing the salt solution. At the same salt concentration, the reduction of Pmax gs and E of the Greeny-type was lower and immediate recovery was observed when the root zone was rinsed. Both clones were irreversibly inhibited after 4 hours of exposure to 40 ppt. Recovery was primarily related to exposure time, as Pmax, gs and E rates of both clones recovered completely after fresh-water rinsing in the 15-minute experiment. The Greeny-type also recovered after the 70-minute exposure, but not the Land-type. We<
Cite this paper: Achenbach, L. and Brix, H. (2014) Monitoring the Short-Term Response to Salt Exposure of Two Genetically Distinct Phragmites australis Clones with Different Salinity Tolerance Levels. American Journal of Plant Sciences, 5, 1098-1109. doi: 10.4236/ajps.2014.58122.

[1]   Brix, H. (1999) Genetic Diversity, Ecophysiology and Growth Dynamics of Reed (Phragmites australis)—Introduction. Aquatic Botany, 64, 179-184.

[2]   Lambertini, C., Gustafsson, M.H.G., Frydenberg, J., Lissner, J., Speranza, M. and Brix, H. (2006) A Phylogeographic Study of the Cosmopolitan Genus Phragmites (Poaceae) based on AFLPs. Plant Systematics and Evolution, 258, 161-182.

[3]   Achenbach, L., Lambertini, C. and Brix, H. (2012) Phenotypic Traits of Phragmites australis Clones Are Not Related to Ploidy Level and Distribution Range. AoB Plants, 2012, Article ID: pls017.

[4]   Clevering, O.A. and Lissner, J. (2000) Taxonomy, Chromosome Numbers, Clonal Diversity and Population Dynamics of Phragmites australis. Aquatic Botany, 66, 249-250.

[5]   Hansen, D.L., Lambertini, C., Jampeetong, A. and Brix, H. (2007) Clone-Specific Differences in Phragmites australis: Effects of Ploidy Level and Geographic Origin. Aquatic Botany, 86, 269-279.

[6]   Kuhl, H., Koppitz, H., Rolletschek, H. and Kohl, J.G. (1999) Clone-Specific Differences in a Phragmites australis Stand I. Morphology, Genetics and Site Description. Aquatic Botany, 64, 235-246.

[7]   Pauca-Comanescu, M., Clevering, O.A., Hanganu, J. and Gridin, M. (1999) Phenotypic Differences among Ploidy Levels of Phragmites australis growing in Romania. Aquatic Botany, 64, 223-234.

[8]   Gorai, M., Ennajeh, M., Khemira, H. and Neffati, M. (2010) Combined Effect of NaCl-Salinity and Hypoxia on Growth, Photosynthesis, Water Relations and Solute Accumulation in Phragmites australis Plants. Flora, 205, 462-470.

[9]   Lessmann, J.M., Brix, H., Bauer, V., Clevering, O.A. and Comin, F.A. (2001) Effect of Climatic Gradients on the Photosynthetic Responses of Four Phragmites australis Populations. Aquatic Botany, 69, 109-126.

[10]   Lissner, J., Schierup, H.H., Comin, F.A. and Astorga, V. (1999) Effect of Climate on the Salt Tolerance of two Phragmites australis Populations. I. Growth, Inorganic Solutes, Nitrogen Relations and Osmoregulation. Aquatic Botany, 64, 317-333.

[11]   Pagter, M., Bragato, C. and Brix, H. (2005) Tolerance and Physiological Responses of Phragmites australis to Water Deficit. Aquatic Botany, 81, 285-299.

[12]   Pagter, M., Bragato, C., Malagoli, M. and Brix, H. (2009) Osmotic and Ionic Effects of NaCl and Na2SO4 Salinity on Phragmites australis. Aquatic Botany, 90, 43-51.

[13]   Vasquez, E.A., Glenn, E.P., Guntenspergen, G.R., Brown, J.J. and Nelson, S.G. (2006) Salt Tolerance and Osmotic Adjustment of Spartina alterniflora (Poaceae) and the Invasive M Haplotype of Phragmites australis (Poaceae) along a Salinity Gradient. American Journal of Botany, 93, 1784-1790.

[14]   Meadows, R.E. and Saltonstall, K. (2007) Distribution of Native and Introduced Phragmites australis in Freshwater and Oligohaline Tidal Marshes of the Delmarva Peninsula and Southern New Jersey. Journal of the Torrey Botanical Society, 134, 99-107.[99:DONAIP]2.0.CO;2

[15]   Saltonstall, K. (2002) Cryptic Invasion by a Non-Native Genotype of the Common Reed, Phragmites australis, into North America. Proceedings of the National Academy of Sciences of the United States of America, 99, 2445-2449.

[16]   Zedler, J. B. and Kercher, S. (2004) Causes and Consequences of Invasive Plants in Wetlands: Opportunities, Opportunists, and Outcomes. Critical Reviews in Plant Sciences, 23, 431-452.

[17]   Hauber, D.P., Saltonstall, K., White, D.A. and Hood, C.S. (2011) Genetic Variation in the Common Reed, Phragmites australis, in the Mississippi River Delta Marshes: Evidence for Multiple Introductions. Estuaries and Coasts, 34, 851-862.

[18]   Lambertini, C., Mendelssohn, I.A., Gustafsson, M.H.G., Olesen, B., Riis, T., Sorrell, B.K. and Brix, H. (2012) Tracing the Origin of Gulf Coast Phragmites (Poaceae): A Story of Long-Distance Dispersal and Hybridization. American Journal of Botany, 99, 538-551.

[19]   Achenbach, L. and Brix, H. (2013) Can Differences in Salinity Tolerance Explain the Distribution of Four Genetically Distinct Lineages of Phragmites australis in the Mississippi River Delta. Hydrobiologia.

[20]   Achenbach, L., Eller, F., Nguyen, L.X. and Brix, H. (2013) Differences in Salinity Tolerance of Genetically Distinct Phragmites australis Clones. AoB Plants, 5, Article ID: plt019.

[21]   Gao, L., Tang, S., Zhuge, L., Nie, M., Zhu, Z., Li, B. and Yang, J. (2012) Spatial Genetic Structure in Natural Populations of Phragmites australis in a Mosaic of Saline Habitats in the Yellow River Delta, China. PLoS ONE, 7, Article ID: e43334.

[22]   Gorai, M., Ennajeh, M., Khemira, H. and Neffati, M. (2011) Influence of NaCl-Salinity on Growth, Photosynthesis, Water Relations and Solute Accumulation in Phragmites australis. Acta Physiologiae Plantarum, 33, 963-971.

[23]   Hanganu, J., Mihail, G. and Coops, H. (1999) Responses of Ecotypes of Phragmites australis to Increased Seawater Influence: A Field Study in the Danube Delta, Romania. Aquatic Botany, 64, 351-358.

[24]   Munns, R. and Tester, M. (2008) Mechanisms of Salinity Tolerance. Annual Review of Plant Biology, 59, 651-681.

[25]   Munns, R. (2002) Comparative Physiology of Salt and Water Stress. Plant, Cell & Environment, 25, 239-250.

[26]   Zhu, J.K. (2002) Salt and Drought Stress Signal Transduction in Plants. Annual Reviews Plant Biology, 53, 247-273.

[27]   Mühling, K.H. and Läuchli, A. (2002) Effect of Salt Stress on Growth and Cation Compartmentation in Leaves of Two Plant Species Differing in Salt Tolerance. Journal of Plant Physiology, 159, 137-146.

[28]   Doering, P.H., Chamberlain, R.H. and McMunigal, J.M. (2001) Effects of Simulated Saltwater Intrusions on the Growth and Survival of Wild Celery, Vallisneria americana, from the Caloosahatchee Estuary (South Florida). Estuaries, 24, 894-903.

[29]   Greenway, H. and Munns, R. (1980) Mechanisms of Salt Tolerance in Non-Halophytes. Annual Review of Plant Physiology and Plant Molecular Biology, 31, 149-190.