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 AJPS  Vol.5 No.7 , March 2014
Effects of Potassium Fertilizer on Water-Soluble Carbohydrate Content of Timothy (Phleum pratense L.), Silage Fermentation, Nutritive Values, and Nutrient Intake
Abstract: The objective of this study was to examine the effects of potassium fertilizer (standard (S) and high (H) levels) on water-soluble carbohydrate (WSC) content of timothy (Phleum pratense L.), silage fermentation, nutritive values, and nutrient intake. The silage treatments were as follows: S level without inoculant (SC), S level plus inoculant (SI), H level without inoculant (HC), and H level plus inoculant (HI). The K content was increased by 14.5% in timothy grown with the H level compared with the S level. The WSC contents of the S and H treatments were 75.9 and 66.1 g·kg-1 dry matter (DM), respectively. The silage fermentation quality was low with both SC and HC treatments. The addition of inoculant significantly improved the fermentation quality in SI and HI treatments. The addition increased the DM and organic matter digestibilities of silage. The total digestible nutrient (TDN) content of silage was highest with the HI treatment. The DM, TDN, and digestible energy intakes with the SI and HI treatments were improved compared with the SC and HC treatments, respectively. This study demonstrated negligible effects of K fertilizer levels and significant effects of the lactic acid bacteria inoculant on the fermentation quality, nutritive values, and feed intake of silage.
Cite this paper: Wang, P. , Souma, K. , Okamoto, H. , Kin, S. , Sugita, A. , Furudate, A. , Sato, C. , Nibe, A. , Cai, Y. and Masuko, T. (2014) Effects of Potassium Fertilizer on Water-Soluble Carbohydrate Content of Timothy (Phleum pratense L.), Silage Fermentation, Nutritive Values, and Nutrient Intake. American Journal of Plant Sciences, 5, 1030-1038. doi: 10.4236/ajps.2014.57115.
References

[1]   McDonal, P., Henderson, A.R. and Heron, S.J.E. (1991) The Biochemistry of Silage. Chalcombe Publications, Aberystwyth, 184-194.

[2]   Masuko, T. (2001) Preparation Technique for Enhancing Fermentation Quality, Nutritive Value and Intake Silage. Japanese Society of Grassland Science, 47, 534-543. http://ci.nii.ac.jp/naid/110003850240

[3]   Masaki, S. and Ohyama, Y. (1976) Nonstructural Carbohydrates in Italian Ryegrass Determined by Liquid Chromatography. Japanese Journal of Zootechnical Science, 47, 205-211. http://dx.doi.org/10.2508/chikusan.47.205

[4]   Souma, K., Masuko, T., Shimizu, C., Yamada, K. and Cai, Y.-M. (2006) Factors Affecting Water Soluble Carbohydrates Content and Sugar Composition of Timothy (Phleum pratense L.), the Leading Grass Species in Hokkaido. Japanese Journal of Zootechnical Science, 77, 495-500. http://dx.doi.org/10.2508/chikusan.77.495

[5]   Okamoto, H., Okumura, M. and Furudate, A. (2007) Recommended Application Rate of Nitrogen Fertilizer for Perennial Ryegrass (Loliun perenne L.) Monoculture Meadow in Tenpoku Region of Hokkaido. Japanese Society of Grassland Science, 52, 243-249. http://ci.nii.ac.jp/naid/110006201772

[6]   Masuko, T., Souma, K., Wang, P., Yamada, K., Ymada, S., Cai, Y.-M. and Nibe, A. (2008) Water Soluble Carbohydrate and Mono and Oligosaccharides Content of Wilted Timothy (Phleum pratense L.) Harvested in the Morning and Evening and Fermentation Quality of Wilted Silage. Nihon Chikusan Gakkaiho, 79, 37-44.
http://dx.doi.org/10.2508/chikusan.79.37

[7]   Bélanger, G. and Richards, J.R. (1997) Growth Analysis of Timothy Grown with Varying N Nutrition. Canadian Journal of Plant Science, 77, 373-380. http://dx.doi.org/10.4141/P96-109

[8]   Bélanger, G. and McQueen, R.E. (1999) Leaf and Stem Nutritive Value of Timothy Grown with Varying N Nutrition on Spring and Summer. Canadian Journal of Plant Science, 79, 223-229. http://dx.doi.org/10.4141/P98-077

[9]   Lovett, D.K., Bortolozzo, A., Conaghan, P., O’Kiely, P. and O’Mara, F.P. (2004) In Vitro Total and Methane Gas Production as Influenced by Rate of Nitrogen Application, Season of Harvest and Perennial Ryegrass Cultivar. Grass and Forage Science, 59, 227-232. http://dx.doi.org/10.1111/j.1365-2494.2004.00421.x

[10]   Tremblay, G.F., Bélanger, G. and Drapeau, R. (2005) Nitrogen Fertilizer Application and Developmental Stage Affect Silage Quality of Timothy (Phleum pratense L.). Grass and Forage Science, 60, 337-355.
http://dx.doi.org/10.1111/j.1365-2494.2005.00483.x

[11]   Nordheim, H. and Nolden, H. (2009) Effect of Maturity Stage, Nitrogen Fertilization and Seasonal Variation on Ruminal Degradation Characteristics of Neutral Detergent Fibre in Timothy (Phleum pratense L.). Animal Feed Science and Technology, 149, 30-59. http://dx.doi.org/10.1016/j.anifeedsci.2008.04.015

[12]   Okamoto, H., Furudate, A. and Masuko, T. (2011) The Effect of Nitrogen Fertilizer on Forage Quality and Silage Fermentation of Timothy (Phleum pratense L.) Pure Sward. Japanese Society of Grassland Science, 56, 274-277.
http://ci.nii.ac.jp/naid/110008460667

[13]   Wang, P., Souma, K., Yano, T., Nakano, M., Okamoto, H., Furudate, A., Cao, B.-H., Sato, C. and Masuko, T. (2009) Influences of Nitrogen Fertilization Level on Timothy Silage Fermentation Quality, Nutritive Value, and Nutrient Intake in Sheep. Proceedings of the 3rd Korea-China-Japan Symposium on Grassland Agriculture Livestock Production, August 2009, 190-191. http://www.dbpia.co.kr/Journal/ArticleDetail/1197354

[14]   Kiso, S., Takahashi, J., Saigusa, T., Hayakawa, Y. and Okumura, M. (2002) Pasture and Forage Crop. In: Hokkaido Food Safety Room of the Hokkaido Agricultural Administration Office, Ed., Hokkkaido Fertilization Guide, Hokkaido Agricultural Administration Office Press, Sapporo, 202-229.

[15]   Maeda, Y. and Tamura, T. (1999) Manual of Livestock Manure Disposal and Usage in 1999. In: Ed Manure Project Team of the Hokkaido Agriculture Livestock Experiment Station, Hokkaido Agricultural Extension Association Press, Sapporo, 21-23.

[16]   Kume, S. (2010) Chapter 6: Mineral. In: Masuko, T., Hanada, M. and Nakatuji, K., Eds., Fundamentals and Applications of Dairy Cattle Nutrition, Dairy Japan Press, Tokyo, 82-90.

[17]   Masuko, T., Souma, K., Shirai, M., Kawai, Y., Furukawa, K., Shibata, H., Hayashikawa, K. and Saigusa, T. (2008) Variations in the Water Soluble Carbohydrate Content of Timothy (Phleum pratense L.) and Soil Chemical Characteristics in the Northern Part of the Kamikawa and Tokachi Areas. Japanese Society of Grassland Science, 54, 257-259.
http://ci.nii.ac.jp/naid/110006990246

[18]   Japan Grassland Agriculture and Forage Seed Association (JGAFSA) (2009) Guide Book for Quality Evaluation of Forage. Japanese Society of Grassland Livestock Seed Science Press, Tokyo, 75-77.

[19]   AOAC (2000) Official Methods of Analysis. 17th Edition Association of Official Analytical Chemists, Gaithersburg.

[20]   Van Soest, P.J., Roberston, J.B. and Lewis, B.A. (1991) Methods for Dietary Fiber, Neural Detergent Fiber, and Non-Starch Polysaccharides in Relation to Animal Nutrition. Journal of Dairy Science, 74, 3583-3597.
http://dx.doi.org/10.3168/jds.S0022-0302(91)78551-2

[21]   Deriaz, R.E. (1961) Routine Analysis of Carbohydrates and Lignin in Herbage. Journal of the Science of Food and Agriculture, 12, 152-160. http://dx.doi.org/10.1002/jsfa.2740120210

[22]   Gotoh, S. (1990) Plant Nutrient Methods. Hakubunkan Press, Tokyo, 134-142.

[23]   Okamoto, H., Furudate, A. and Yoshida, M. (2007) Effect of Nitrogen Fertilization on Sugar Composition and Silage Fermentation of Perennial Ryegrass (Lolium perenne L.). Japanese Society of Grassland Science, 53, 195-200.
http://ci.nii.ac.jp/naid/110006448994

[24]   Barker, S.B. and Summerson, W.H. (1941) The Colorimetric Determination of Lactic Acid in Biological Material. Journal of Biological Chemistry, 138, 535-554. http://www.jbc.org/content/138/2/535.full.pdf

[25]   Kageyama, K., Mori, H. and Sato, K. (1973) Simultaneous Determination of Volatile Fatty Acid and Lactic Acid in Silage by Gas Chromatography. Japanese Journal of Zootechnical Science, 44, 465-469.
http://dx.doi.org/10.2508/chikusan.44.465

[26]   Robinson, P.H., Tamminga, S. and Van Vuuren, A.M. (1986) Influence of Declining Level of Feed Intake and Varying the Proportion of Starch in the Concentrate on Rumen Fermentation in Dairy Cows. Livestock Production Science, 15, 173-189. http://dx.doi.org/10.1016/0301-6226(86)90026-6

[27]   Burmester, C.H., Mullins, G.L. and Ball, D.M. (1991) Potassium Fertilization Effects on Yield and Longevity of Established Alfalfa. Communications in Soil Science and Plan Analysis, 22, 19-20.
http://dx.doi.org/10.1080/00103629109368557

[28]   Li, X.-K., Lu, J.-W. and Huang, Y.-F. (2007) Effect of Different Potassium Fertilizers on the Yield and Nutrients Uptake of Sudangrass-Ryegrass Rotate Crop System. Pratacultural Science, 24, 72-76.
http://dx.doi.org/10.3969/j.issn.1001-0629.2007.07.017

[29]   Walker, D.J., Black, C.R. and Miller, A.J. (1998) The Role of Cytosolic Potassium and pH in the Growth of Barley Roots. Plant Physiology, 118, 957-964. http://dx.doi.org/10.1104/pp.118.3.957

[30]   Gerardeaux, E., Saur, E., Constantin, J., Porte, A. and Meille, L.J. (2009) Effect of Carbon Assimilation on Dry Weight Production and Partitioning during Vegetative Growth. Plant Soil, 324, 329-343.
http://dx.doi.org/10.1007/s11104-009-9950-z

[31]   Takahashi, E. (2005) Essential Elements, In: Yamazaki, K., Ed., Plant Nutrition and Fertilizer, Asakura-Shoten Press, Tokyo, 80-81.

[32]   Ojima, K. and Odashima, M. (1970) Physiological Studies on Carbohydrates of Forage Plants 4. Fructosan of Leaf Sheathes of Several Perennial Grasses. Japanese Society of Grassland Science, 16, 119-123.
http://ci.nii.ac.jp/naid/110006440141

[33]   Yoshida, M. and Tamura, K. (2011) Research on Fructan in Wheat and Temperate Forage Grasses in Japan. Japan Agricultural Research Quarterly, 45, 9-14. http://dx.doi.org/10.6090/jarq.45.9

[34]   Udén, P. (2010) The Influence of Sample Preparation of Forage Crops and Silages on Recovery of Soluble and Non-Structural Carbohydrates and Their Predictions by Fourier Transform Mid-IR. Animal Feed Science and Technology, 160, 49-61. http://dx.doi.org/10.1016/j.anifeedsci.2010.06.013

[35]   Masuko, T., Okamoto, H., Wang, P. and Souma, K. (2009) Approach to the Preparation of High Quality Grass Silage. Japanese Society of Grassland Science, 55, 56-68. http://ci.nii.ac.jp/naid/110007227766

[36]   Souma, K., Masuko, T., Wang, P., Tsutsumi, H., Hara, Y., Hanada, M. and Okamoto, M. (2008) Influences of Feeding Grass Silage Added with Formic Acid, a Bacterial Inoculant, and a Mixture of Bacterial Inoculant and Enzyme on Nutritive Value and Nutrient Intake in Sheep. Japanese Society of Grassland Science, 53, 270-276.
http://ci.nii.ac.jp/naid/110006572035

[37]   Gordon, F.J. (1989) An Evaluation through Lactating Cattle of a Bacterial Inoculant as an Additive for Grass Silage. Grass and Forage Science, 44, 169-179. http://dx.doi.org/10.1111/j.1365-2494.1989.tb01924.x

[38]   Gordon, F.J. (1989) A Further Study on the Evaluation through Lactating Cattle of a Bacterial Inoculant as an Additive for Grass Silage. Grass and Forage Science, 44, 353-357. http:dx.doi.org/10.1111/j.1365-2494.1989.tb02174.x

[39]   Anderson, R., Gracey, H.I., Kennedy, S.J., Unsworth, E.F. and Steen, R.W.J. (1989) Evaluation Studies in the Development of a Commercial Bacterial Inoculant as an Additive for Grass Silage. 1. Using Pilot-Scale Tower Silos. Grass and Forage Science, 44, 361-369. http://dx.doi.org/10.1111/j.1365-2494.1989.tb01934.x

[40]   Mcleod, D.S., Wilkins, R.J. and Raymond, W.F. (1970) The Voluntary Intake by Sheep and Cattle of Silages Differing in Free-Acid Content. Journal of Agricultural Science, 75, 311-319. http://dx.doi.org/10.1017/S0021859600017007

[41]   Wilkins, R.J., Wilson, R.F. and Harris, C.E. (1971) The Voluntary Intake of Silage by Sheep. I. Interrelationships between Silage Composition and Intake. Journal of Agricultural Science, 77, 531-537.
http://dx.doi.org/10.1017/S0021859600064613

[42]   Hutchinson, K.J. and Wilkins, R.J. (1971) The Voluntary Intake of Silage by Sheep: II. The Effects of Acetate on Silage Intake. Journal of Agricultural Science, 77, 539-543. http://dx.doi.org/10.1017/S0021859600064625

[43]   Kikuchi, M., Shibata, F. and Oshima, S. (1975) Influence of Direct Addition of Ammonium Carbonate to Grass Silage on the Voluntary Intake of Cattle. Japanese Journal of Zootechnical Science, 46, 722-723.
http://dx.doi.org/10.2508/chikusan.46.722

 
 
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