MSA  Vol.5 No.3 , March 2014
Comparative Toxicity of “Tin Free” Self-Polishing Copolymer Antifouling Paints and Their Inhibitory Effects on Larval Development of a Non-Target Organism
ABSTRACT

Toxic substances released as a result of leaching from painted surfaces to the aquatic environment affect both fouling organisms and non-target biota. Artemia fransiscana nauplii have been considered a useful test system for the examination of toxicity for antifouling paints. In this study, we examined the effect of four tin free self-polishing copolymer (SPC) antifouling paints on the larval development of Artemia nauplii. Based on the L(S/V)50 values the order of toxicity of the antifouling paints was: ANTI F > SHARKSKIN > OCEAN T/F > MICRON. Furthermore, the body size of Artemia nauplii was significantly affected at lethal and above lethal L(S/V)5024h values. The body size of 48 h-aged nauplii exposed for the last 24 hours to each of the four SPC antifouling paints was significantly lower than that of the 48 h-aged controls (0.88 ± 0.030 mm). In addition, the body size of 72 h-aged nauplii maintained for the last 24 hours to pure synthetic seawater after exposure for 24 hours to each of the four SPC antifouling paints was significantly lower than that of the 72 h-aged controls (0.96 ±0.027 mm). Overall, the SPCs examined here were substantially toxic to Artemia nauplii, but with different toxicities and modes of action, as a result of the synergistic action of distinct components of the antifouling paints.


Cite this paper
Castritsi-Catharios, J. , Alambritis, G. , Miliou, H. , Cotou, E. and Zouganelis, G. (2014) Comparative Toxicity of “Tin Free” Self-Polishing Copolymer Antifouling Paints and Their Inhibitory Effects on Larval Development of a Non-Target Organism. Materials Sciences and Applications, 5, 158-169. doi: 10.4236/msa.2014.53020.
References
[1]   Dvorák, P., Zdársky M. and Beňová, K. (2009) Possibilities of the Alternative Generation II Biotests at Artemia. Interdisciplinary Toxicology, 2, 45-47.

[2]   Yebra, D.M., Kill, S. and Dam-Johansen, K. (2004) Antifouling Technology: Past, Present and Future Steps towards Efficient and Environmentally Friendly Antifouling Coatings. Progress in Organic Coatings, 50, 75-104.
http://dx.doi.org/10.1016/j.porgcoat.2003.06.001

[3]   Gerigk, U., Schneider, U. and Stewen, U. (1998) The Present Status of TBT Copolymer Antifouling Paints versus TBT-Free Technology. Preprints of Extended Abstracts, ACS National Meeting, 38, 91-94.

[4]   Smith, B.S. (1981) Male Characteristics on Female Mud Snails Caused by Antifouling Bottom Paints. Journal of Applied Toxicology, 1, 22-25. http://dx.doi.org/10.1002/jat.2550010106

[5]   Bryan, G.W., Gibbs, P.E., Hummerstone, L.G. and Burt, G.R. (1986) The Decline of the Gastropod Nucella Lapillus around South-West England: Evidence for the Effect of Tributyltin from Anti-Fouling Paints. Journal of the Marine Biological Association of the United Kingdom, 66, 611-640. http://dx.doi.org/10.1017/S0025315400042247

[6]   Fent, K. (1996) Ecotoxicology of Organotin Compounds. Critical Reviews in Toxicology, 26, 1-117.
http://dx.doi.org/10.3109/10408449609089891

[7]   Vos, J.G., Dybing, E., Greim, H.A., Ladefoged, O., Lambre, C., Tarazona, J.V., Brandt, I. and Vethaak, A.D. (2000) Health Effects of Endocrine-Disrupting Chemicals on Wildlife, with Special Reference to the European Situation. Critical Reviews in Toxicology, 30, 71-133. http://dx.doi.org/10.3109/10408449609089891

[8]   Dimitriou, P., Castritsi-Catharios, J. and Miliou, H. (2003) Acute Toxicity Effects of Tributyltin Chloride and Triphenyltin Chloride on Gilthead Seabream, Sparus aurata L., Embryos. Ecotoxicology and Environmental Safety, 54, 30-35. http://dx.doi.org/10.1016/S0147-6513(02)00008-8

[9]   Mee, L.D. and Fowler, S.W. (1991) Organotin Biocides in the Marine Environment: A Managed Transient? Marine Environmental Research, 32, 1-5. http://dx.doi.org/10.1016/S0147-6513(02)00008-8

[10]   Morcillo, Y., Borghi, V. and Porte, C. (1997) Survey of Organotin Compounds in the Western Mediterranean Using Molluscs and Fish as Sentinel Organisms. Archives of Environmental Contamination and Toxicology, 32, 198-203.
http://dx.doi.org/10.1007/s002449900175

[11]   Shawky, S. and Emons, H. (1998) Distribution Pattern of Organotin Compounds at Different Trophic Levels of Aquatic Ecosystems. Chemosphere, 36, 523-535. http://dx.doi.org/10.1007/s002449900175

[12]   (2002) Focus on IMO: Antifouling Systems. International Maritime Organization, London.

[13]   Avai, T., Harino, H., Ohji, M. and Langston, W.J. (2009) Ecotoxicology of Antifouling Biocides. Springer, Springer Verlag, Berlin.

[14]   Thomas, K.V. (2001) The Environmental Fate and Behavior of Antifouling Paint Booster Biocides: A Review. Biofouling, 17, 73-86. http://dx.doi.org/10.1080/08927010109378466

[15]   Konstantinou, I.K. and Albanis, T.A. (2004) Worldwide Occurrence and Effects of Antifouling Paint Booster Biocides in the Aquatic Environment: A Review. Environment International, 30, 235-248.
http://dx.doi.org/10.1016/S0160-4120(03)00176-4

[16]   Karlsson, J. and Eklund, B. (2004) New Biocide-Free and Anti-Fouling Paints Are Toxic. Marine Pollution Bulletin, 49, 456-464. http://dx.doi.org/10.1016/j.marpolbul.2004.02.034

[17]   Mochida, K., Ito, K., Harino, H., Kakuno, A. and Fujii, K. (2006) Acute Toxicity of Pyrithione Antifouling Biocides and Joint Toxicity with Copper to Red Sea Bream (Pagrus major) and Toy Shrimp (Heptacarpus futilirostris). Environmental Toxicology and Chemistry, 25, 3058-3064. http://dx.doi.org/10.1897/05-688R.1

[18]   Gatidou, T.G. and Thomaidis, N.S. (2007) Evaluation of Single and Joint Toxic Effects of Two Antifouling Biocides, Their Main Metabolites and Copper Using Phytoplankton Bioassays. Aquatic Toxicology, 85, 185-191.
http://dx.doi.org/10.1016/j.aquatox.2007.09.002

[19]   Karlsson, J., Ytreberg, E. and Eklund, B. (2010) Toxicity of Anti-Fouling Paints for Use on Ships and Leisure Boats to Non-Target Organisms Representing Three Trophic Levels. Environmental Pollution, 158, 681-687.
http://dx.doi.org/10.1016/j.envpol.2009.10.024

[20]   (1998) Concerning the Placing of Biocidal Products on the Market. European Council Directive 98/9/EC, Brussels.

[21]   Okamura, H., Aoyama, I., Liu, D., Maquire, R.J., Pacepavivius, G.I. and Lau, Y.L. (2000) Fate and Ecotoxicity of the New Antifouling Compound Irgasol 1051 in the Aquatic Environment. Water Research, 34, 3523-3530.
http://dx.doi.org/10.1016/S0043-1354(00)00095-6

[22]   Férnandez-Alba, A.R., Hernando, M.D., Piedra, L. and Chisti, Y. (2002) Toxicity Evaluation of Single and Mixed Anti-Fouling Biocides Measured with Acute Toxicity Bioassays. Analytica Chimica Acta, 456, 303-312.
http://dx.doi.org/10.1016/S0043-1354(00)00095-6

[23]   Grunnet, K.S. and Dahllof, I. (2005) Environmental Fate of the Antifouling Compound Zinc Pyrithione in Seawater. Environmental Toxicology and Chemistry, 24, 3001-3006. http://dx.doi.org/10.1897/04-627R.1

[24]   Myers, J.H., Gunthorpe, L., Allinson, G. and Duda, S. (2006) Effects of Antifouling Biocides to the Germination and Growth of the Marine macroalga, Hormosira banksii (Turner) Desicaine. Marine Pollution Bulletin, 52, 1048-1055.
http://dx.doi.org/10.1016/j.marpolbul.2006.01.010

[25]   Thouvenin, M., Peron, J.J., Langlois, V., Guerrin, P., Langlois, J.Y. and Vallee-Rehel, K. (2002) Formulation and Antifouling Activity of Marine Paints: A Study by a Statistically Based Experiments Plan. Progress in Organic Coatings, 44, 85-92. http://dx.doi.org/10.1016/S0300-9440(01)00247-8

[26]   Thouvenin, M., Peron, J.J., Charreteur, C., Guerrin, P., Langlois, J.Y. and Vallee-Rehel, K. (2002) A Study of the Biocide Release from Antifouling Paints. Progress in Organic Coatings, 44, 75-83.
http://dx.doi.org/10.1016/S0300-9440(01)00246-6

[27]   Karlsson, J., Breitholtz, M. and Eklund, B. (2006) A Practical Ranking System to Compare Toxicity of Anti-Fouling Paints. Marine Pollution Bulletin, 52, 1661-1667. http://dx.doi.org/10.1016/j.marpolbul.2006.06.007

[28]   Kill, S., Johansen-Dam, K., Weinell, C.E. and Pedersen, M.S. (2002) Seawater-Soluble Pigments and Their Potential Use in Self-Polishing Antifouling Paints: Simulation-Based Screening Tool. Progress in Organic Coating, 45, 423434. http://dx.doi.org/10.1016/S0300-9440(02)00146-7

[29]   Katranitsas, A., Castritsi-Catharios, J. and Persoone, G. (2003) The Effects of a Copper-Based Antifouling Paint on Mortality and Enzymatic Activity of a Non-Target Marine Organism. Marine Pollution Bulletin, 46, 1491-1494.
http://dx.doi.org/10.1016/S0025-326X(03)00253-4

[30]   Loschau, M. and Kratke, R. (2005) Efficacy and Toxicity of Self-Polishing Biocide-Free Antifouling Paints. Environmental Pollution, 138, 260-267. http://dx.doi.org/10.1016/S0025-326X(03)00253-4

[31]   Castritsi-Catharios, J., Bourdaniotis, N. and Persoone, G. (2007) A New Simple Method with High Precision for Determining the Toxicity of Antifouling Paints on Brine Shrimp Larvae (Artemia): First Results. Chemosphere, 67, 1127-1132. http://dx.doi.org/10.1016/j.chemosphere.2006.11.033

[32]   Brix, K.V., Gerdes, R.M., Adams, W.J. and Grosell, M. (2006) Effects of Copper, Cadmium, and Zinc on the Hatching Success of Brine Shrimp (Artemia franciscana). Archives of Environmental Contamination and Toxicology, 51, 580-583. http://dx.doi.org/10.1007/s00244-005-0244-z

[33]   Barahona, M.V. and Sanchez-Fortun, S. (1999) Toxicity of Carbamates to the Brine Shrimp Artemia salina and the Effect of Atropine, BW284c51, iso-OMPA and 2-PAM on carbaryl toxicity. Environmental Pollution, 104, 469-476.
http://dx.doi.org/10.1016/S0269-7491(98)00152-3

[34]   Persoone, G. and Castritsi-Catharios, J. (1987) A Simple Bioassay with Artemia Larvae to Determine the Acute Toxicity of Antifouling Paints. Water Research, 23, 893-897.

[35]   Castritsi-Catharios, J., Katsorchis, T., Marakis, S., Koukoulis, D. and Kafetzidakis, A. (1987) Acute Toxicity Tests with Dispersants and Oil Dispersant Mixtures. MAP Technical Reports Series (UNEP) No. 10/UNEP, Mediterranean Action Plan, F.A.O., Rome, 89-107.

[36]   Di Toro, D.M., Allen, H.E., Bergman, H.L., Meyer, J.S., Paquin, P.R. and Santore, R.C. (2001) Biotic Ligand Model of the Acute Toxicity of Metals. 1. Technical basis. Environmental Toxicology and Chemistry, 20, 2383-2396.
http://dx.doi.org/10.1002/etc.5620201034

[37]   Santore, R.C., Di Toro, D.M., Paquin, P.R. and Allen, H.E. (2001) Biotic Ligand Model of the Acute Toxicity of Metals. 2. Application to Acute Copper Toxicity in Freshwater Fish and Daphnia. Environmental Toxicology and Chemistry, 20, 2397-2402. http://dx.doi.org/10.1897/1551-5028(2001)020<2397:BLMOTA>2.0.CO;2

[38]   Koutsaftis, A. and Aoyama, I. (2008) Toxicity of Diuron and Copper Pyrithione on the Brine Shrimp, Artemia franciscana: The Effects of Temperature and Salinity. Journal of Environmental Science and Health, Part A, 43, 1581-1585.
http://dx.doi.org/10.1080/10934520802329794

[39]   Koutsaftis, A. and Aoyama, I. (2007) Toxicity of Four Antifouling Biocides and Their Mixtures on the Brine Shrimp Artemia salina. Science of the Total Environment, 387, 166-174. http://dx.doi.org/10.1016/j.scitotenv.2007.07.023

[40]   Koutsaftis, A. and Aoyama, I. (2006) The Interactive Effects of Binary Mixtures of Three Antifouling Biocides and Three Heavy Metals against the Marine Algae Chaetoceros gracilis. Environmental Toxicology, 21, 432-439.
http://dx.doi.org/10.1002/tox.20202

[41]   Xu, X., Wang, X., Li, Y., Wang, Y. and Wang, Y. (2011) Acute Toxicity and Synergism of Binary Mixtures of Antifouling Biocides with Heavy Metals to Embryos of Sea Urchin Glyptocidaris crenularis. Human and Experimental Toxicology, 30, 1013-1021.

[42]   Chambers, L.D., Stokes, K.R., Walsh, F.C. and Wood, R.J.K. (2006) Modern Approaches to Marine Antifouling Coatings. Surface and Coating Technology, 201, 3642-3652. http://dx.doi.org/10.1016/j.surfcoat.2006.08.129

[43]   Townsin, R.L. (2003) The Ship Hull Fouling Penalty. Biofouling, 19, 9-15.
http://dx.doi.org/10.1080/0892701031000088535

[44]   Yebra, D.M., Kiil, S., Dam-Johansen, K. and Weinell, K. (2005) Reaction Rate Estimation of Controlled Release Antifouling Paint Binders: Rosin Based Systems. Progress in Organic Coatings, 53, 256-275.
http://dx.doi.org/10.1016/j.porgcoat.2005.03.008

[45]   Kamaya, Y., Tokita, N. and Suzuki, K. (2005) Effects of Dehydroabietic Acid and Abietic Acid on Survival, Reproduction, and Growth of the Crustacean Daphnia magna. Ecotoxicology and Environmental Safety, 61, 83-88.
http://dx.doi.org/10.1016/j.ecoenv.2004.07.007

[46]   Omae, I. (2003) Organotin Antifouling Paints and Their Alternatives. Applied Organometalic Chemistry, 17, 81-105.
http://dx.doi.org/10.1002/aoc.396

[47]   Callow, M.E. and Willingham, G.L. (1996) Degradation of Antifouling Biocides. Biofouling, 10, 239-249.
http://dx.doi.org/10.1080/08927019609386283

[48]   Okamura, H. (2002) Photodegradation of the Antifouling Compounds Irgarol 1051 and Diuron Released from a Commercial Antifouling Paint. Chemosphere, 48, 43-50. http://dx.doi.org/10.1016/S0045-6535(02)00025-5

[49]   Comber, S.D.W., Gardner, M.J. and Boxall, A.B.A. (2002) Survey of Four Marine Antifoulant Constituents (copper, Zinc, Diuron and Irgarol 1051) in Two UK Estuaries. Journal of Environmental Monitoring, 4, 417-425.
http://dx.doi.org/10.1039/b202019j

[50]   Alyuruk, H. and Cavas, L. (2013) Toxicities of Diuron and Irgarol on the Hatchability and Early Stage Development of Artemia salina. Turkish Journal of Biology, 37, 151-157.

[51]   Callow, M.E. and Finlay, J.A. (1995) A Simple Method to Evaluate the Potential for Degradation of Antifouling Biocides. Biofouling, 9, 153-165. http://dx.doi.org/10.1080/08927019509378299

[52]   Voulvoulis, N., Scrimshaw, M.D. and Lester, J.N. (2000) Occurrence of Four Biocides Utilized in Antifouling Paints, as Alternatives to Organotin Compounds in Waters and Sediments of a Commercial Estuary in the UK. Marine Pollution Bulletin, 40, 938-946. http://dx.doi.org/10.1016/S0025-326X(00)00034-5

[53]   Voulvoulis, N., Scrimshaw, M.D. and Lester, J.N. (2002) Partitioning of Selected Antifouling Biocides in the Aquatic Environment. Marine Environmental Research, 53, 1-16. http://dx.doi.org/10.1016/S0141-1136(01)00102-7

[54]   Hamwijk, C., Schouten, A., Foekema, E.M., Ravensberg, J.C., Collombon, M.T., Schmidt, K. and Kugler, M. (2005) Monitoring of the Booster Biocide Dichlofluanid in Water and Marine Sediment of Greek Marinas. Chemosphere, 60, 1316-1324. http://dx.doi.org/10.1016/j.chemosphere.2005.01.072

[55]   White, S.L. and Rainbow, P.S. (1982) Regulation and Accumulation of Copper, Zinc and Cadmium by the Shrimp Palaemon elegans. Marine Ecolology Progress Series, 8, 95-101. http://dx.doi.org/10.3354/meps008095

[56]   Santos, M.H.S., da Cunda, N.T. and Bianchini, A. (2000) Effects of Copper and Zinc on Growth, Feeding and Oxygen Consumption of Farfantepenaeus paulensis Postlarvae (Decapoda: Penaeidae). Journal of Experimental Marine Biology and Ecology, 247, 233-242. http://dx.doi.org/10.1016/S0022-0981(00)00152-0

[57]   Nebeker, A.V. and Schuytema, G.S. (1998) Chronic Effects of the Herbicide Diuron on Freshwater Cladocerans, Amphipods, Midges, Minnows, Worms and Snails. Archives of Environmental Contamination and Toxicology, 35, 441446. http://dx.doi.org/10.1007/s002449900400

[58]   Kerster, H.W. and Schaeffer, D.J. (1983) Brine Shrimp (Artemia salina) Nauplii as a Teratogen Test System. Ecotoxicology and Environmental Safety, 7, 342-349. http://dx.doi.org/10.1016/0147-6513(83)90079-9

[59]   Castritsi-Catharios, J., Syriou, V., Miliou, H. and Zouganelis, G.D. (2013) Toxicity Effects of Bisphenol A to the Nauplii of the Brine Shrimp Artemia franciscana. Journal of Biological Research-Thessaloniki, 19, 38-45.

 
 
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