JEP  Vol.5 No.4 , March 2014
Responses of Aquatic Vegetation to Pollution: Preliminary Results on Ecotoxicological Effects and Bioenrichment Factors
Abstract: This study evaluates, on a preliminary basis, the principal morpho-physiological effects induced by pollution stress in four aquatic vegetation species of great ecological relevance in transitional water ecosystems. Three macroalgae (Chaetomorpha linum, Valonia aegagrophyla, Graciliariopsis longissima) and one phanerogams species (Ruppia cirrhosa) were exposed to different doses of trace elements (Cu, Hg, Zn) and surfactants (dodecylbenzenesulfonic acid sodium salt) in laboratory controlled microcosm conditions and morpho-physiological responses (photosynthetic complex alteration, percentage of died cells, morphological changes) were measured before and after 7 and 14 days of exposure. Levels of pollutants in tissues and BioConcentration Factors (BCF) for each species were calculated after 14 days of exposure. Results suggest that photosynthetic complex alteration can be a useful tool to evaluate early and sub-lethal significant changes due to exposure to pollution stress in all of the considered species. A clear species-specificity was observed concerning trace element levels in tissues after 14 days of exposure, while dose-dependent behavior was observed for BCFs.
Cite this paper: Renzi, M. , Giovani, A. and Focardi, S. (2014) Responses of Aquatic Vegetation to Pollution: Preliminary Results on Ecotoxicological Effects and Bioenrichment Factors. Journal of Environmental Protection, 5, 274-288. doi: 10.4236/jep.2014.54031.

[1]   Volpi, G.A., Girardini, M., Marchetto, D. and Pantani, C. (2009) Microtox® Solid Phase Test: Effect of Diluents Used in Toxicity Test. Ecotoxicology and Environmental Safety, 72, 851-886.

[2]   USEPA (1974) Marine Algal Assay Procedure Bottle Test. Eutrophication and Lake Restoration Branch. National Environmental Research Center, Corvallis, Oregon, 43.

[3]   Trainor, F.R. (1984) Indicator Algal Assays: Laboratory and Field Approaches. In: Shubert, L.E., Ed., Algae as Ecological Indicators, Academic Press, London, 3-14.

[4]   UNI EN ISO 10253 (2006) Water Quality—Marine Algal Growth Inhibition Test with Skeletonema costatum and Phaeodactylum tricornutum.

[5]   Giusti, E., Marsili-Libelli, S., Renzi, M. and Focardi, S. (2010) Assessment of Spatial Distribution of Submerged Vegetation in the Orbetello Lagoon by Means of a Mathematical Model. Ecological Modelling, 221, 1484-1493.

[6]   Renzi, M., Guerranti, C., Giovani, A., Perra, G. and Focardi, S.E. (2013) Perfluorinated Compounds: Levels, Trophic Web Enrichments and Human Dietary Intakes in Transitional Water Ecosystems. Marine Pollution Bulletin.

[7]   EEC (2000) Directive 2000/60/EC of the European Parliament and of the Council Establishing a Framework for Community Action in the Field of Water Policy. OJEC L 327/1-72, Brussels.

[8]   Lytle, J.S. and Lytle, T.F. (2001) Use of Plants for Toxicity Assessment of Estuarine Ecosystems. Environmental Toxicology and Chemistry, 20, 68-83.

[9]   Geider, R.J., Roche, J.L., Greene, R.M. and Olalzola, M. (1993) Response of the Photosynthetic Apparatus of Pheodactylum tricornutum (Bacillariophyceae) to Nitrate, Phosphate or Iron Starvation. Journal of Phycology, 29, 755-766.

[10]   Ronen, R. and Galun, M. (1984) Pigment Extraction from Lichens with Dimethylsulfoxide (DMSO) and Estimation of Chlorophyll Degradation. Environmental and Experimental Botany, 24, 239-245.

[11]   Renzi, M., Roselli, L., Giovani, A., Focardi, S.E. and Basset, A. (in Press) An Integrated Approach to Ecotoxicity Tests on Unicellular Algal Species: The Case Study of Phaeodactylum tricornutum, Ecotoxicology.

[12]   Brandini, F.P., Da Silva, E.T., Pellizzari, F.P., Fonseca, A.L.O. and Fernandes, L.F. (2001) Production and Biomass Accumulation of Periphytic Diatom Growing on Glass Slides during a 1-Year Cycle in a Subtropical Estuarine Environment (Bay of Paranaguá, Southern Brazil). Marine Biology, 138, 163-171.

[13]   Moreira, S.M., Guilhermino, L. and Ribeiro, R. (2006) An in Situ Assay with the Microalga Phaeodactylum tricornutum for Sediment-Overlying Water Toxicity Evaluations in Estuaries. Environmental Contamination and Toxicology, 25, 2272-2279.

[14]   Dalsgard, T. and Krause-Jensen, D. (2006) Monitoring Nutrient Release from Fish Farms with Macroalgal and Phytoplankton Bioassays. Aquaculture, 256, 302-320.

[15]   Macinnis-Ng, C.M.O. and Ralph, P.J. (2003) In Situ Impact of Petrochemicals on the Photosynthesis of the Seagrass Zostera capricorni. Marine Pollution Bulletin, 46, 1395-1407.

[16]   Ralph, P.J., Gademann, R. and Dennison, W.C. (1998) In Situ Seagrass Photosynthesis Measured Using a Submersible, Pulse-Amplitude Modulated Fluorometer. Marine Biology, 132, 367-373.

[17]   Durako, M.J. and Kunzelman, J.I. (2002) Photosynthetic Characteristics of Thalassia testudinum Measured in Situ by Pulse-Amplitude Modulated (PAM) Fluorimetry: Methodological and Scale-Based Considerations. Aquatic Botany, 73, 173-185.

[18]   Renzi, M., Giovani, A. and Focardi, S.E. (2012) Water Pollution by Surfactants: Fluctuations Due to Tourism Exploitation in a Lagoon Ecosystem. Journal of Environmental Protection, 3, 1004-1009.

[19]   Renzi, M., Perra, G., Guerranti, C., Mariottini, M., Baroni, D., Volterrani, M., Graziosi, M., Specchiulli, A. and Focardi, S. (2009) Assessment of Environmental Pollutants in Ten Southern-Italian Harbours Sediments. Toxicology and Industrial Health, 25, 351-363.

[20]   Renzi, M., Tozzi, A., Baroni, D. and Focardi, S. (2011) Factors Affecting the Distribution of Trace Elements in Harbour Sediments. Chemistry and Ecology, 27, 235-250.

[21]   Cantarero, S., Camino-Sánchez, F.J., Zafra-Gómez, A., Ballesteros, O., Navalón, A., Vílchez, J.L., Verge, C., Reis, M.S. and Saraiva, P.M. (2012) Evaluation of the Presence of Major Anionic Surfactants in Marine Sediments. Marine Pollution Bulletin, 64, 587-594.

[22]   Meyer, J.S., Davidson, W., Sundby, B., Oris, J.T., Lauren, D.J., Forstner, U., Hong, J. and Crosby, D.G.S. (1994) The Effects of Variable Redox Potentials, pH, and Light on Bioavailability in Dynamic Water-Sediment Environments. In: Landrum, P.F., Bergman, H.L. and Benson, W.H., Eds., Bioavailability, Physical, Chemical, and Biological Interactions, Lewis Publications, Boca Raton, 155-170.

[23]   Bradley, J.C. (2004) Toxicity of Anionic Surfactants in a Primary Effluent: Identification, Characterization and Removal. Thesis of Master of Applied Science.

[24]   OECD (1996) Bioconcentration: Flow-Through Fish Test. OECD Guidelines for the Testing Chemicals No. 305E and Compliance Monitoring No. 1. Organization for Economic Co-Operation and Development, Paris, 41.

[25]   Wellburn, A.R. (1994) The Spectral Determination of Chlorophylls A and B, as Well as Total Carotenoids, Using Various Solvents with Spectrophotometers of Different Resolution. Journal of Plant Physics, 144, 307-313.

[26]   Crippen, R.W. and Perrier, J.L. (1974) The Use of Neutral Red and Evan’s Blue for Live-Dead Determination of Marine Phytoplankton. Stain Technology, 49, 97-104.

[27]   Gallagher, J.C. (1984) Patterns of Cell Viability in the Diatoms, Skeletonema costatum in Batch Culture and in Natural Populations. Estuaries, 7, 98-101.

[28]   USEPA (SW-846) (1996) Test Methods for Evaluating Solid Waste, Physical/Chemical Methods, Method 3051A, “Microwave Assisted Acid Digestion of Sediments, Sludge, Soils and Oils”. Office of Solid Waste, Washington DC.

[29]   USEPA (SW-846) (1996) Test Methods for Evaluating Solid Waste, Physical/Chemical Methods, Method 6010B “Inductively Coupled Plasma-Atomic Emission Spectrometry”. Office of Solid Waste, Washington DC.

[30]   US-EPA (1996) SW-846, Test Methods for Evaluating Solid Waste, Physical/Chemical Methods, Method 7473 Mercury in Solids and Solutions by Thermal Decomposition, Amalgamation, and Atomic Absorption Spectrophotometry. Office of Solid Waste, Washington DC.

[31]   USEPA (1996) Fish BCF. US Environmental Protection Agency, Washington DC.

[32]   Mohammady, N.G.E.D., Chen, Y.C., El-Mahdy, A.E.R.A. and Mohammad, R.F. (2005) Temporal Alterations of Nannochloropsis salina (Eustigmatophyceae) Grown under Aqueous Diesel Fuel Stress. Journal of Applied Phycology, 17, 161-170.

[33]   Bacci, E. (1996) Ecotoxicology of Organic Contaminants. Elsevier, Amsterdam, 150 p.

[34]   Truhaut, R. (1975) Ecotoxicology—A New Branch of Toxicology: A General Survey of Its Aims, Methods and Prospects. In: McIntyre, A.D. and Mills, C.F., Eds., Ecological Toxicology Research, Plenum Press, New York, 3-23.

[35]   Jochem, F.J. (2000) Probing the Physiological State of Phytoplankton at Single Cell Level. Scientia Marina, 64, 183-195.

[36]   Stauber, J.L., Franklin, N.M. and Adams, M.S. (2002) Applications of Flow Cytometry to Ecotoxicity Testing Using Microalgae. Trends in Biotechnology, 20, 141-143.

[37]   Eisentraeger, A., Klein, W. and Hahn, J.S. (2003) Comparative Studies on Algal Toxicity Testing Using Fluorometric Microplate and Erlenmeyer Flask Growth Inhibition Assays. Ecotoxicology and Environmental Safety, 54, 346-354.

[38]   Moreira-Santos, M., Soares, A.M. and Ribeiro, R. (2004) A Phytoplankton Growth Assay for Routine in Situ Environmental Assessments. Environmental Toxicology and Chemistry, 23, 1549-1560.

[39]   Costanza, R., d’Arge, R., de Groot, R., Farber, S., Grasso, M., Hannon, B., Limburg, K., Naeem, S., O‘Neill, R.V., Paruelo, J., Raskin, R.G. and Sutton, P. (1997) The Value of the World’s Ecosystem Services and Natural Capital. Nature, 387, 253-260.

[40]   Boudouresque, C.F. and Meinesz, A. (1982) Découverte de l’herbier de Posidonies. Cahier Parc nation. Port-Cros, 4, 1-79.

[41]   Clarke, S.M. and Kirkman, H. (1989) Seagrasss Dynamics. In: Larkum, A.W.D., McComb, A.J. and Shepherd, S.A., Eds., Biology of Seagrasses, Vol. 2, Aquatic Plant Studies, 610-634.

[42]   Decree Law n. 152 (2006) Norme in materia ambientale. Gazzetta Ufficiale n. 88, 14th 2006-Supplemento Ordinario n. 96. Document in Italian Language.

[43]   Orfanidis, S., Papathanasiou, V., Sabetta, L., Pinna, M., Gigi, V., Gounaris, S., Tsiagga, E., Nakou, K. and Theodosiou, T.H. (2007) Benthic Macrophyte Communities as Bioindicators of Transitional and Coastal Waters: Relevant Approaches and Tools. Transitional Waters Bulletin, 3, 45-49.

[44]   Streiber, U., Muller, J.F., Haugg, A. and Gademann, R. (2002) New Type of Dual-Channel PAM Chlorophyll Fluorometer for Highly Sensitive Water Toxicity Biotests. Photosynthesis Research, 74, 317-330.

[45]   Katsumata, M., Koike, T., Nishikawa, M., Kazumura, K. and Tsuchiya, H. (2006) Rapid Ecotoxicological Bioassay Using Delayed Fluorescence in the Green Alga Pseudokirchneriella subcapitata. Water Research, 40, 3393-3400.

[46]   Yamane, A., Okada, M. and Sudo, R. (1984) The Growth Inhibition of Planktonic Algae Due to Surfactants Used in Washing Agents. Water Research, 18, 1101-1105.

[47]   Nyberg, H. (1988) Growth of Selenastrum capricornutum in the Presence of Synthetic Surfactants. Water Research, 22, 217-223.

[48]   Dirilgen, N. and Ince, N. (1994) Inhibition Effect of Anionic Surfactant SDS on Duckweed, Lemna minor with Considerations of Growth and Accumulation. Chemosphere, 31, 4185-4196.

[49]   Pergent-Martini, C. and Le Ravallec, C. (2007) Guidelines for Impact Assessment on Seagrass Meadows. Regional Activity Centre for Specially Protected Areas (RAC/SPA) Boulevard du leader Yasser Arafat B.P. 337-1080 Tunis CEDEX, 48.

[50]   Stauber, J.L. and Florence, T.M. (1990) Mechanisms of Toxicity of Zinc to the Marine Diatom Nitzschia closterium. Marine Biology, 105, 519-524.

[51]   Nguyen-Deroche, T.L.N., Le, T.T., Bui, T.V., Rincé, Y., Tremblin, G. and Morant-Manceau, A. (2009) Effects of Copper on Growth and Photosynthesis in Marine Diatoms: A Comparison between Species from Two Different Geographical Areas. Cryptogamie Algologie, 30, 97-109.

[52]   Li, M., Hu, C., Zhu, Q., Chen, L., Kong, Z. and Liu, Z. (2006) Copper and Zinc Induction of Lipid Peroxidation and Effects on Antioxidant Enzyme Activities in the Microalga Pavlova viridis (Prymnesiophyceae). Chemosphere, 62, 565-572.

[53]   Rijstenbil, J.W. (2003) Effects of UVB Radiation and Salt Stress on Growth, Pigments and Antioxidative Defence of the Marine Diatom Cylindrotheca closterium. Marine Ecology Progress Series, 254, 37-48.

[54]   Steeman-Nielsen, L. and Wium Andersen, S. (1971) The Influence of Cu on Photosynthesis and Growth in Diatoms. Physiologia Plantarum, 24, 480-484.

[55]   Rai, L.C., Gaur, J.P. and Kumar, H.D. (1981) Phycology and Heavy Metal Pollution. Biological Reviews, 56, 99-151.

[56]   Sunda, G.W. and Huntsman, S.A. (1983) Effect of Competitive Interactions between Manganese and Copper on Cellular Manganese and Growth in Estuarine and Oceanic Species of the Diatom Thalassiosira. Limnology and Oceanography, 28, 924-934.

[57]   Monnier-Besombes, G. (1983) Etude de la contamination de la Posidonie (Posidonia oceanica L. Delile) et de son milieu par des composants de détergents synthétiques. Thèse Doct. 3ème cycle Ecol., Univ. Aix-Marseille II, Fr., 1-162.

[58]   Brinkhuis, B.H., Penello, W.F. and Churchill, A.C. (1980) Cadmium and Manganese Flux in Eelgrass Zoostera marina II. Metal Uptake by Leaf and Root-Rhizome Tissues. Marine Biology, 58, 187-196.

[59]   Ward, T.J. (1987) Temporal Variation of Metals in the Seagrass Posidonia australis and Its Potential as a Sentinel Accumulator near a Lead Smelter. Marine Biology, 95, 315-321.

[60]   Ward, T.J. (1989) The Accumulation and Effects of Metals in Seagrasses Habitats. In: Larkum, A.W.D., McComb, A.J. and Shepherd, S.A., Eds., Biology of Seagrasses, Elsevier, Amsterdam, 797-820.

[61]   Schlacher-Hoenlinger, M.A. and Schlacher, T.A. (1998) Accumulation, Contamination and Seasonal Variability of Trace Metals in the Coastal Zone—Patterns in a Seagrass Meadow from the Mediterranean. Marine Biology, 131, 401-410.

[62]   Arnot, J.A. and Gobas, F.A.P.C. (2006) A Review of Bioconcentration Factor (BCF) and Bioaccumulation Factor (BAF) Assessments for Organic Chemicals in Aquatic Organisms. Environmental Reviews, 14, 257-297.