AJPS  Vol.5 No.6 , March 2014
Color of Corn Grains and Carotenoid Profile of Importance for Human Health
Abstract: The increasing on the levels of carotenoids in staple foods of broad human consumption is one of the strategies of food biofortification programs, mainly due to the importance of these compounds to human health on the prevention of vitamin A deficiency. Maize is a major staple food due to its high consumption in regions where problems of Vitamin A deficiency are of great relevance. Maize biofortification programs have made progress in determining the amounts of carotenoids in grain of thousands of accesses. This work aimed at studying the influence of the color of the grains in the profile of carotenoids in four different Brazilian genotypes. The selection of ears within the same genotype was based on a color scale, considering the lighter (lightest yellow) in one group and the most colorful (darkest orange) in another group. Significant interactions (p < 0.05) between the color of the grains and the genotypes for all the variables were detected in addition to genetic variability for both groups (lightest yellow and darkest orange). The colored ears of corn showed a high level of total carotenoids (TC) and fractions in RS 535 and RS 445, and the colorful ears of genotype RS 535 showed 300% more α + β carotenes (μg·g-1) in relation to lighter of this same material. The results of this study showed the influence of genotype on the grain color and content of carotenoids, indicating that breeders have the flexibility to make selection of some genotypes based on grain color, reducing cost and time compared to laboratory methods used for the screening of genetic materials.
Cite this paper: Almeida Rios, S. , Dias Paes, M. , Cardoso, W. , Borém, A. and Teixeira, F. (2014) Color of Corn Grains and Carotenoid Profile of Importance for Human Health. American Journal of Plant Sciences, 5, 857-862. doi: 10.4236/ajps.2014.56099.

[1]   Britton, G. (2005) Structure and Properties of Carotenoids in Relation to Function. FASEB Journal, 9, 1551-1558.

[2]   Rodriguez-Amaya, D.B. and Kimura, M. (2004) Harvest Plus Handbook for Carotenoid Analysis. Washington, DC and Cali: IFPRI and CIAT, 58. (Harvest Plus Technical Monograph, 2).

[3]   Rios, S.A., Paes, M.C.D., Abreu, S.C. and Cardoso, W.S. (2011) Deficiências Nutricionais e a Biofortificação de Alimentos. In: Borém, A. and Rios, S.A., Eds., Milho Biofortificado, Suprema, Visconde do Rio Branco, 9-21.

[4]   Pelissari, F.M., Rona, M.S.S. and Matioli, G. (2008) O Licopeno e suas Contribuições na Prevenção de Doenças. Arquivos do Mudi, 12, 5-11.

[5]   FAO. Chapter 7 (2002) Vitamin A. World Health Organization for Food and Agriculture Organization of the United Nations Rome.

[6]   Vallabhaneni, R. and Wurtzel, E.T. (2009) Timing and Biosynthetic Potential for Carotenoid Accumulation in Genetically Diverse Germplasm of Maize. Plant Physiology, 150, 562-572.

[7]   Rodriguez-Amaya, D.B. (2001) A Guide to Carotenoid Analysis in Foods. ILSI Human Nutrition Institute. One Thomas Circle, NW, Washington DC, 20005-5802, 64.

[8]   CIMMYT (2013) Milho Biofortificado.

[9]   Kurilich, A.C. and Juvik J.A. (1999) Quantification of Carotenoid and Tocopherol Antioxidants in Zea mays. Journal of Agricultural and Food Chemistry, 47, 1948.

[10]   Egesel, C.O., Wong, J.C., Lambert, R.J. and Rocheford, T.R. (2003) Combining Ability of Maize Inbreds for Carotenoids and Tocopherols. Crop Science, 43, 818-823.

[11]   Egesel, C.O., Wong, J.C., Lambert, R.J. and Rocheford, T.R. (2004) Gene Dosage Effects on Carotenoid Concentration in Maize Grain. Maydica, 48, 183-190.

[12]   Rios, S.A. (2008) Interação Genótipos x Ambientes para Carotenóides em Cultivares de Milho. 65 f. Ph.D. dissertation, Universidade Federal de Viçosa, Viçosa.

[13]   Palaisa, K.A., Morgante, M., Williams, M. and Rafalski, A. (2003) Contrasting Effects of Selection on Sequence Diversity and Linkage Disequilibrium at Two Phytoene Synthase Loci. The Plant Cell, 15, 1795-1806.

[14]   Chandler, K., Lipka, A.E., Owens, B.F., Li, H., Buckler, E.S., Rocheford, T. and Gore, M.A. (2013) Genetic Analysis of Visually Scored Orange Kernel Color in Maize. Crop Science, 53, 189-200.

[15]   Magalhães, P.C., Durães, F.O.M., Carneiro, N.P. and Paiva, E. (2002) Fisiologia do Milho. Sete Lagoas: EMBRAPACNPMS, 23 (Embrapa—CNPMS. Circular Técnica 22).

[16]   American Association of Cereal Chemists (2000) Approved Methods of the AACC, 10th Ed. Approved Methods 44-15A and 6-13. The Association: St. Paul, St. Paul, Minnesota.

[17]   Cruz, C.D. (2006) Programa Genes—Estatística Experimental e Matrizes. 1. ed. Viçosa: Editora UFV, 1, 285.

[18]   Kandianis, C.B., Stevens, R., Liu, W., Palacios, N., Montgomery, K., Pixley, K., White, W.S. and Rocheford, T. (2013) Genetic Architecture Controlling Variation in Grain Carotenoid Composition and Concentrations in Two Maize Populations. Theoretical and Applied Genetics, 126, 2879-2895.

[19]   USDA—Nutrient Database. (2013)

[20]   Cardoso, W.S., Paes, M.C.D., Galvão, J.C.C., Rios, S.A., Guimarães, P.E.O., Schaffert, R.E. and Borém, A. (2009) Variability of Maize Genotypes for Grain Carotenoid Composition. Variabilidade de Genótipos de Milho Quanto à Composição de Carotenoides nos Grãos. Pesquisa Agropecuária Brasileira, Brasileira, 44, 164-173.

[21]   Derera, J., Pillay, K., Siwela, M. and Veldman, F.J. (2011) Consumer Acceptance of Yellow, Provitamin A-Biofortified Maize in KwaZulu-Natal. South African Journal of Clinical Nutrition, 24, 186-191.

[22]   Meenakshi, J.V., Banerji, A., Manyong, V., Tomlins, K., Mittal, N. and Hamukwala, P. (2012) Using a Discrete Choice Experiment to Elicit the Demand for a Nutritious Food: Willingness-to-Pay for Orange Maize in Rural Zambia. Journal of Health Economics, 31, 62-71.

[23]   Stevens, R. and Winter-Nelson, A. (2008) Consumer Acceptance of Provitamin A-biofortified Maize in Maputo, Mozambique. Food Policy, 33, 341-351.

[24]   Harjes, C.E., Rocheford, T.R., Bai, L., Brutnell, T., Kandianis, C.B., Sowinski S.G., et al. (2008) Natural Genetic Variation in Lycopene Epsilon Cyclase Tapped for Maize Biofortification. Science, 319, 330-333.

[25]   Quackenbush, F.W., Firch, J.G., Rabourn, W.J., McQuistan, M., Petzold, W.N. and Kargl, T.E. (1961) Composition of corn, Analysis of Carotenoids in Corn Grain. Journal of Agricultural and Food Chemistry, 9, 132-135.

[26]   Weber, E. (1987) Carotenoids and Tocols of Corn Grain Determined by HPLC. Journal of American Oil Chemistry Society, 64, 1129-1134.

[27]   Yan, J., Kandianis, C.B., Harjes, C.E., Bai, L., Kim, E.-H., Yang, X., Skinner, D.J., Fu, Z., Mitchell, S., Li, Q., Fernandez, M.G.S., Zaharieva, M., Babu, R., Fu, Y., Palacios, N., Li, J., DellaPenna, D., Brutnell, T., Buckler, E.S., Warburton, M.L. and Rocheford, T. (2010) Rare Genetic Variation at Zea mays crtRB1 Increases b-Carotene in Maize Grain. Nature Genetics, 42, 322-327.