Analysis of Genetic Diversity among Wild and Cultivated Chickpea Genotypes Employing ISSR and RAPD Markers

Pramod Kumar  Singh; Himanshu  Sharma; Nidhi  Srivastava; Sameer S.  Bhagyawant

doi:10.4236/ajps.2014.55082

Pramod Kumar Singh, Himanshu Sharma, Nidhi Srivastava, Sameer S. Bhagyawant^*

Abstract: Chickpea (Cicer airetinum L.) is an important and most preferred food legume in many parts of the world especially in the Indian sub-continent. Molecular analysis of chickpea using DNA technology has been carried out to identify the diverse genetic base of the cultivars for selected preferential introductions as efficient edible elements. A few of these genetic stocks have been documented here to tap their genetic diversity. The status shows that the level of polymorphism in this species is low. Using PCR based markers, marker assisted selection of polymorphy is one of the established techniques. Here, this procedure has been employed to expedite gene/QTL pyramiding in the chickpea. The study presented here includes analysis of 12 germplasms of chickpea. Standard CTAB method has been performed, with certain modifications, to get better intensity and resolution of DNA bands. Extracted DNA, amplified with 41 RAPD and 21 ISSR primers are thereby tested to determine genetic diversity. The presentation discusses the results of chickpea germplasm diversity on the basis of these observations.

Keywords: Chickpea; Randomly Amplified Polymorphic DNA and Inter Simple Sequence Repeats; Genetic Diversity

Cite this paper: Singh, P. , Sharma, H. , Srivastava, N. and Bhagyawant, S. (2014) Analysis of Genetic Diversity among Wild and Cultivated Chickpea Genotypes Employing ISSR and RAPD Markers. American Journal of Plant Sciences, 5, 676-682. doi: 10.4236/ajps.2014.55082.

References

[1] Ahmad, F., Khan, A.I., Awan, F.S., Sadia, B., Sadaqat, H.A. and Bahadur, S. (2010) Genetic Diversity of Chickpea (Cicer arietinum L.) Germplasm in Pakistan as Revealed by RAPD Analysis. Genetics and Molecular Research, 9, 1414-1420. http://dx.doi.org/10.4238/vol9-3gmr862

[2] William, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A. and Tingey, S.V. (1990) DNA Polymorphisms Amplified by Arbitrary Primers Are Useful as Genetic Markers. Nucleic Acids Research, 18, 6531-6535.
http://dx.doi.org/10.1093/nar/18.22.6531

[3] Welsh, J. and Mcclelland, M. (1990) Fingerprinting Genomes Using PCR with Arbitrary Primers. Nucleic Acids Research, 18, 7213-7218. http://dx.doi.org/10.1093/nar/18.24.7213

[4] Khan, S., Qureshi, M.I., Kamaluddin, T.A. and Abdin, M.Z. (2007) Protocol for Isolation of Genomic DNA from Dry and Fresh Roots of Medicinal Plants Suitable for RAPD and Restriction Digestion. African Journal of Biotechnology, 6, 175-178.

[5] Bhagyawant, S.S. and Srivastava, N. (2008) Genetic Fingerprinting of Chickpea (Cicer arietinum L.) Germplasm Using ISSR Markers and Their Relationships. African Journal of Biotechnology, 7, 4428-4431.

[6] Souframanien, J. and Gopalakrishna, T.G. (2004) A Comparative Analysis of Genetic Diversity in Black Gram Genotypes Using RAPD and ISSR Markers. Theoretical and Applied Genetics, 109, 1687-1693.
http://dx.doi.org/10.1007/s00122-004-1797-3

[7] Dikshit, H.K., Jhang, T., Singh, N.K., Koundal, K.R. Bansal, K.C., Chandra, N., Tickoo, J.L. and Sharma, T.R. (2007) Genetic Differentiation of Vigna Species by RAPD, URP and SSR Markers. Biologia Plantarum, 3, 451-457.
http://dx.doi.org/10.1007/s10535-007-0095-8

[8] Ravi, M., Geethanjali, S., Sammeyafarheen, F. and Maheswaran, M. (2003) Molecular Marker Based Genetic Diversity Analysis in Rice (Oryza sativa L.) Using RAPD and SSR Markers. Euphytica, 133, 243-253.
http://dx.doi.org/10.1023/A:1025513111279

[9] Iruela, M., Rubio, J., Cubero, J.I., Gil, J. and Millán, T. (2002) Phylogenetic Analysis in the Genus Cicer and Cultivated Chickpea Using RAPD and ISSR Markers. Theoretical and Applied Genetics, 104, 643-651.
http://dx.doi.org/10.1007/s001220100751

[10] Rao, L., Usha, R.P., Deshmukh, P., Kumar, P. and Panguluri, S. (2007) RAPD and ISSR Finger-Printing in Cultivated Chickpea (Cicer arietinum L.) and Its Wild Progenitor Cicer reticulatum Ladizinsky. Genetic Resources and Crop Evolution, 54, 1235-1244. http://dx.doi.org/10.1007/s10722-006-9104-6

[11] Talebi, R., Naji, A.M. and Fayaz, F. (2008) Geographical Patterns of Genetic Diversity in Cultivated Chickpea (Cicer arietinum L.) Characterized by Amplified Fragment Length Polymorphism. Plant Soil Environment, 54, 447-452.

[12] Agrawal, P.K. and Srivastava, A. (2010) Assessment of genetic Diversity among Chickpea Cultivars of India Using RAPD Marker. Indian Journal of Human Genetics, 70, 264-270.

[13] Pakseresht, F., Talebi, R. and Karami, E. (2013) Comprative Assessment of ISSR, DAMD and SCoT Markers for Evaluation of Genetic Diversity and Conservation of Landrace Chickpea (Cicer arietinum L.) Genotypes Collected from North-West of Iran. Physiology and Molecular Biology of Plants, 19, 563-574.
http://dx.doi.org/10.1007/s12298-013-0181-7

[14] Virk, P.S., Newbury, H.J., Jacckson, M.T. and Ford-Lioyd, B.V. (1995) The Identification of Duplicate Accessions within a Rice Germplasm Collection Using RAPD Analysis. Theoretical and Applied Genetics, 90, 1049-1055.
http://dx.doi.org/10.1007/BF00222920

[15] Abbo, S., Berger, J. and Turner, N.C. (2003) Evaluation of Cultivated Chickpea: Four Bottlenecks Limit Diversity and Constrain Adaptation. Functional Plant Biology, 30, 1081-1087.

[16] Jamil, F.F., Sarwar, M., Sarwar, N., Khan, J.A., Zahid, M.H., Yousaf, S., Arshad, H.M.I. and Haq, I.U. (2010) Genotyping with RAPD Markers Resolves Pathotype Diversity in the Ascochyta blight and Fusarium wilt Pathogen of Chickpea in Pakistan. Pakistan Journal of Botany, 42, 1369-1378.