AS  Vol.5 No.4 , March 2014
Genotypic Variation for Tolerance to Low Soil Phosphorous in Common Bean under Controlled Screen House Conditions

Production of common bean (Phaseolus vulgaris) is often limited by the low availability of soil phosphorus (P). Identification of common bean genotypes adapted to low phosphorus (P) availability may be a feasible strategy to overcome the poor plant growth and production in P-deficient soils. Genetic variation for P response of thirteen common bean genotypes was studied under screen house controlled conditions using triple super phosphate as P source. The common bean genotypes varied in leaf area, shoot mass, root mass, total root length, basal and lateral roots production, shoot P concentration and P uptake under phosphorous deficiency and high phosphorous. All the measured variables were significantly correlated with each other, which in turn were correlated to P uptake. Generally the large-seeded genotypes RWR 1946 and RWR 2075 appeared to have the best growth, hence superior P efficiency under low P availability, while at the same time they were more responsive to added P. These results complement the earlier field based observed tolerance to low soil phosphorous of the selected genotypes under the BILFA strategy.

Cite this paper: Namayanja, A. , Semoka, J. , Buruchara, R. , Nchimbi, S. and Waswa, M. (2014) Genotypic Variation for Tolerance to Low Soil Phosphorous in Common Bean under Controlled Screen House Conditions. Agricultural Sciences, 5, 270-285. doi: 10.4236/as.2014.54030.

[1]   Beebe, S.E., Rojas-Pierce, M., Yan, X.L., Blair, M.W., Pedraza, F. and Munoz, F. (2006) Quantitative Trait Loci for Root Architecture Traits Correlated with Phosphorus Acquisition in Common Bean. Crop Science, 46, 413-423.

[2]   Allen, D.J., Ampofo, J.K. and Wortmann, C.S. (1996) Pests, Diseases and Nutrition Disorders of Common Bean in Africa. A Field Guide, 96.

[3]   Wortman, C.S., Kirkby, R.A., Eledu, C.A. and Allen, D.J. (1998) Atlas of Common Bean (Phaseolus vulgaris L.) Production in Africa. CIAT, Cali, 131.

[4]   Schachtman, D.P., Reid, R.J. and Ayling, S.M. (1998) Update on Phosphorus Uptake. 689 Phosphorus Uptake by Plants: From Soil to Cell. Plant Phyisiology, 116, 447-453.

[5]   Hinsinger, P. (2001) Bioavailability of Soil Inorganic P in the Rhizosphere as Affected by Root-Induced Chemical Changes: A Review. Plant Soil, 237, 173-195.

[6]   Driessen, P., Deckers, S., Spaargaren, O. and Nachtergaele, F. (2001) Lecture Notes on the Major Soils of the World. FAO, Rome.

[7]   Fairhust, T., Lefroy, E., Mutert, E. and Batjes, N. (1999) The Importance, Distribution and Causes of Phosphorus Deficiency as a Constraint to Crop Production in the Tropics. Agroforestry Forum, 9, 2-8.

[8]   Araujo, A.P., Ferreira, A.I. and Grande Teixeira, M. (2005) Inheritance of Root Traits and Phosphorous Uptake in Common Bean (Phaseolus vulgaris) under Limited Soil Phosphorous Supply. Euphytica, 145, 1-2, 33-40.

[9]   Tesfaye, M., Liu, J.Q., Allan, D.L. and Vance, C.P. (2007) Genomic and Genetic Control of Phosphate Stress in Legumes. Plant Physiology, 144, 594-603.

[10]   Shenoy, V.V. and Kalagudi, G.M. (2005) Enhancing Plant Phosphorus Use Efficiency for Sustainable Cropping. Biotechnology Advances, 23, 501-513.

[11]   Lynch, J.P. (2007) Roots of the Second Green Revolution. Australian Journal of Botany, 55, 493-512.

[12]   Fageria, N.K., Baligar, V.C. and Li, Y.C. (2008) The Role of Nutrient Efficient Plants in Improving Crop Yields in the Twenty First Century. Journal of Plant Nutrition, 31, 1121-1157.

[13]   Lynch, J.P. and Beebe, S.E. (1995) Adaptation of Beans (Phaseolus vulgaris L.) to Low Phosphorus Availability. Horticulture Science, 30, 1165-1171.

[14]   Gahoonia, T.S. and Nielsen, N.E. (2003) Phosphorus Uptake and Growth of a Root Hairless Barley Mutant (Bald Root Barley, BRB) and Wild Type in Low- and High-P Soils. Plant Cell Environment, 26, 1759-1766.

[15]   Yan, X.L., Wu, P., Ling, H.Q., Xu, G.H., Xu, F.S. and Zhang, Q.F. (2006) Plant Nutriomics in China: An Overview. Annals of Botany, 98, 473-482.

[16]   Beebe, S., Lynch J., Galwey, N., Tohme, J. and Ochoa, I. (1997) A Geographical Approach to Identify Phosphorus-Efficient Genotypes among Landraces and Wild Ancestors of Common Bean. Euphytica, 95, 325-336.

[17]   Singh, S.P., Teran, H., Munoz, C.G., Oscorno, J.M., Takegami, J.C. and Thung, M.D.T. (2003) Low Soil Fertility Tolerance in Landraces and Improved Common Bean Genotypes. Crop Science, 43, 110-119.

[18]   Cichy, K.A.C., Blair, M.W., Mendoza, C.H.G., Snapp, S.S. and Kelly, J.D. (2009) QTL Analysis of Root Architecture Traits and Low Phosphorous Tolerance in an Andean Bean Population. Crop Science, 49, 59-68.

[19]   Beebe, S., Rao, I.M., Blair, M.W. and Butare, L. (2009) Breeding for Abiotic Stress Tolerance in Common Bean: Present and Future Challenges. Proceedings of the 14th Australian Plant Breeding and 11th Society for the Advancement of Breeding Research in Asia and Oceania (SABRAO) Conference, Brisbane, 10-14 August 2009.

[20]   Yan, X.L., Beebe, S.E. and Lynch, J.P. (1995) Genetic Variation for Phosphorus Efficiency of Common Bean in Contrasting Soil Types: II. Yield Response. Crop Science, 35, 1094-1099.

[21]   Miller, C.R., Ochoa, I., Nielsen, K.L., Beck, D. and Lynch, J.P. (2003) Genetic Variation for Adventitious Rooting in Response to Low Phosphorus Availability: Potential Utility for Phosphorus Acquisition from Stratified Soils. Functional Plant Biology, 30, 973-985.

[22]   CIAT (2000) Inheritance of Low Phosphorus Tolerance in the Andean Population AND696 × G19833. Bean Improvement for the Tropics Unit Annual Report. CIAT, Cali.

[23]   Wortman, C.S., Lunze L., Ochwoh, V.A. and Lynch, J.P. (1995) Bean Improvement for Low Soil Fertility Soils in Africa. African Crop Science Journal, 3, 469-477.

[24]   Lunze, L., Kimani, P.M., Ndakidemi, P., Rabary, B., Rachier, G.O., Ugen, M.M. and Nabahungu, L. (2002) Selection of Bean Lines Tolerant to Low Soil Fertility Conditions in Africa. Bean Improvement Cooperative, 45, 182-183.

[25]   Lunze, L., Abang, M.M., Buruchara, R., Ugen, M.A., Nabahungu, N.L., Rachier, G.O., Ngongo, M. and Rao, I. (2012) Integrated Soil Fertility Management in Bean-Based Cropping Systems of Eastern, Central and Southern Africa, Soil Fertility Improvement and Integrated Nutrient Management—A Global Perspective. Whalen J., Ed., InTech. eastern- central-and-southern

[26]   Kimani, P., Buruchara, R. and Lubanga, L. (2006) Enhancing the Resilience of Agroecosystems in Central Africa through Improved, Nutrient Dense and Marketable Bean Germplasm Tolerant to Low Fertility Acid Soils. CIALCA Second Planning Workshop, Kigali, 28 August-1 September 2006.

[27]   Namayanja, A., Tukamuhabwa, P., Opio, F., Ugen, M.A., Kimani, P.M., Babirye, A., Kitinda, X., Kabayi, P. and Takusewanya, R. (2003) Selection for Low Soil Fertility Bean Tolerant to Root Rot. Bean Improvement Co-Operative, 46, 95-96.

[28]   PABRA (Pan Africa, Bean Research Alliance) (2007) Narrative Annual Report 2006-2007.

[29]   Bhatt, M. and Chanda, S.V. (2003) Prediction of Leaf Area in Phaseolus vulgaris by Non-Destructive Method. Bulgaria Journal of Plant Physiology, 29, 96-100.

[30]   Vieira, R.F., Carneiro, J.E.S. and Lynch, J.P. (2008) Root Traits of Common Bean Genotypes Used in Breeding Programs for Disease Resistance. Pesquisa Agropecuária Brasileira, 43.

[31]   Murphy, J. and Riley, J.P. (1962) A Modified Single Solution Method for the Determination of Phosphate in Natural Waters. Analytica Chimica Acta, 27, 31-36.

[32]   Broughton, W.J., Hernández, G., Blair, M., Beebe, S., Gepts, P. and Vanderleyden, J. (2003) Beans (Phaseolus spp.): Model Food Legume. Plant and Soil, 252, 55-128.

[33]   Ochoa, I.E., Blair, M.W. and Lynch, J.P. (2006) QTL Analysis of Adventitious Root Formation in Common Bean under Contrasting Phosphorous Availability. Crop Science, 46, 1609-1621.

[34]   Trindade, R.S., Araújo, A.P. and Teixeira, M.G. (2010) Leaf Area of Common Bean Genotypes during Early Pod Filling as Related to Plant Adaptation to Limited Phosphorus Supply. Revista Brasileira de Ciência do Solo, 34, 115-124.

[35]   Lynch, J.P., Lauchli, A. and Epstein, E. (1991) Vegetative Growth of the Common Bean in Response to Phosphorus Nutrition. Crop Science, 31, 380-387.

[36]   Oliveira, A.A.R. (1995) Mycorrhizal Effects on the Growth of Common Bean (Phaseolus vulgaris L.). PhD Thesis, Leeds University, Leeds.

[37]   Radian, J.W. and Eidenbock, M.P. (1984) Hydraulic Conductance as a Factor Limiting Leaf Expansion of Phosphorus-Deficient Cotton Plants. Plant Physiology, 76, 392-394.

[38]   Fredeen, A.L., Rao, I.M. and Terry, N. (1989) Influence of Phosphorus Nutrition on Growth and Carbon Partitioning on Glycine max. Plant Physiology, 89, 225-223.

[39]   Rodríguez, D., Keltjens, W.G. and Goudriaan, J. (1998) Plant Leaf Area Expansion and Assimilate Production in Wheat (Triticum aestivum L.) Growing under Low Phosphorus Conditions. Plant Soil, 200, 227-240.

[40]   Fist, A.J. and Edwards, D.G. (1987) External Phosphorus Requirements of Five Tropical Grain Legumes Grown in Flowing-Solution Culture. Plant and Soil, 99, 75-84.

[41]   Brouwer, R. (1962) Nutritive Influences on the Distribution of Dry Matter in the Plants. Netherlands Journal of Agricultural Sciences, 10, 399-408.

[42]   Borch, K., Bouma, T.J., Lynch, J.P. and Brown, K.M. (1999) Ethylene: A Regulator of Root Architectural Responses to Soil Phosphorus Availability. Plant Cell and Environment, 22, 425-431.

[43]   Lynch, J.P. and Brown, K.M. (2001) Topsoil Foraging—An Architectural Adaptation of Plants to Low Phosphorus Availability. Plant and Soil, 237, 225-237.

[44]   Kim, H.J., Lynch, J.P. and Brown, K.M. (2008) Ethylene Insensitivity Impedes a Subset of Responses to Phosphorus Deficiency in Tomato and Petunia. Plant Cell and Environment, 31, 1744-1755.

[45]   Lambers, H., Finnegan, P.M. and Laliberté, E. (2011) Phosphorus Nutrition of Proteaceae in Severely Phosphorus-Impoverished Soils: Are There Lessons to Be Learned for Future Crops? Plant Physiology, 156, 1058-1066.

[46]   Jin, J., Tang, C.X., Armstrong, R. and Sale, P. (2012) Phosphorus Supply Enhances the Response of Legumes to Elevated CO2 (FACE) in a Phosphorus-Deficient Vertisol. Plant and Soil, 358, 86-99.

[47]   Niu, Y.F., Chai, R.S., Jin, G.L., Wang, H., Tang, C.X. and Zhang, Y.S. (2012) Responses of Root Architecture Development to Low Phosphorus Availability: A Review. Annals of Botany, 112, 391-408.

[48]   Zhu, J. and Lynch, J.L. (2004) The Contribution of Lateral Rooting to Phosphorus Acquisition Efficiency in Maize (Zea mays L.) Seedlings. Functional Plant Biology, 31, 949-958.

[49]   Zhu, J.M., Kaeppler S.M. and Lynch J.P. (2005) Mapping of QTL Controlling Root Hair Length in Maize (Zea mays L.) under Phosphorus Deficiency. Plant and Soil, 270, 299-310.

[50]   Pérez-Torres, C.A., López-Bucio, J. and Cruz-Ramírez, A. (2008) Phosphate Availability Alters Lateral Root Development in Arabidopsis by Modulating Auxin Sensitivity via a Mechanism Involving the TIR1 Auxin Receptor. The Plant Cell, 20, 3258-3272.

[51]   Liao, H., Rubio, G., Yan, X., Cao, A., Brown, K.M. and Lynch, J.P. (2001) Effect of Phosphorus Availability on Basal Root Shallowness in Common Bean. Plant Soil, 232, 69-79.

[52]   Williamson, L.C., Ribrioux, S.P.C.P., Fitter, A.H. and Leyser, H.M.O. (2001) Phosphate Availability Regulates Root System Architecture in Arabidopsis. Plant Physiology, 126, 875-882.

[53]   Bates, T.R. and Lynch, J.P. (1996) Stimulation of Root Hair Elongation in Arabidopsis thaliana by Low Phosphorus Availability. Plant Cell and Environment, 19, 529-538.

[54]   Pérez-Torres, C.A., López-Bucio, J. and Cruz-Ramírez, A. (2008) Phosphate Availability Alters Lateral Root Development in Arabidopsis by Modulating Auxin Sensitivity via a Mechanism Involving the TIR1 Auxin Receptor. The Plant Cell, 20, 3258-3272.

[55]   Péret, B., Clément, M., Nussaume, L. and Desnos T. (2011) Root Developmental Adaptation to Phosphate Starvation: Better Safe than Sorry. Trends in Plant Science, 16, 442-450.

[56]   Tyburski, J., Dunajska-Ordak, K., Skorupa, M. and Tretyn, A. (2012) Role of Ascorbate in the Regulation of the Arabidopsis Thaliana Root Growth by Phosphate Availability. Journal of Botany, 2012, 580342.

[57]   Lynch, J.P. and Van Beem, J.J. (1993) Growth and Architecture of Seedling Roots of Common Bean Genotypes. Crop Science, 33, 1253-1257.