AJAC  Vol.5 No.4 , March 2014
Determination of Melamine in Quail Egg and Milk with Capillary Zone Electrophoresis
Abstract: A capillary zone electrophoresis method was developed for determination of melamine in food samples, such as quail egg and milk products. The CE procedure was performed on fused silica capillary (41 cm × 75 μm I.D.) at 17 kV using pH3.1 60mmol/L phosphate buffer as run buffer and detecting at 200 nm. The proposed method showed good linearity (0.5 - 10.0 μmol/L) and low LOD (0.5 μmol/L) with good reproducibility (RSD% was 2.4 and 3.2 for migration time and peak area respectively), which made it suitable for quantity control of the related food product.
Cite this paper: Kong, Y. , Wei, C. , Wang, Z. , Hou, Z. , Li, H. , Yu, J. , Yuan, J. , Zhao, Y. , Long, J. , Tang, Y. and Gao, M. (2014) Determination of Melamine in Quail Egg and Milk with Capillary Zone Electrophoresis. American Journal of Analytical Chemistry, 5, 258-265. doi: 10.4236/ajac.2014.54032.

[1]   Rovner, S.L. (2008) Chinese Baby-Food Crisis Widens.

[2]   Brown, C.A., Poppenga, R.H., Puschner, B., Miller, D.M., Ellis, A.E., Kang, K.I., Sum, S., Cistola, A.M. and Brown, S.A. (2007) Outbreaks of Renal Failure Associated with Melamine and Cyanuric Acid in Dogs and Cats in 2004 and 2007. Journal of Veterinary Diagnostic Investigation, 19, 525-531.

[3]   Dobson, R.L.M., Quijano, M., Cambron, R.T., Baker, T.R., Pullen, A.M., Regg, B.T., Bigalow-Kern, A.S., Vennard, T., Fix, A., Reimschuessel, R., Overmann, G., Shan, Y. and Daston, G.P. (2008) Identification and Characterization of Toxicity of Contaminants in Pet Food Leading to an Outbreak of Renal Toxicity in Cats and Dogs. Toxicological Sciences, 106, 251-262.

[4]   Goscinny, S., Hanot, V., Halbardier, J.F., Michelet, J.Y. and Van Loco, J. (2011) Rapid Analysis of Melamine Residue in Milk, Milk Products, Bakery Goods and Flour by Ultra-Performance Liquid Chromatography/Tandem Mass Spectrometry: From Food Crisis to Accreditation. Food Control, 22, 226-230.

[5]   Karbiwnyk, C.M., Turnipseed, S.B., Storey, J.M., Madson, M.R., Miller, K.E., Gieseker, C.M., Miller, R.A., Rummel, N.G. and Reimschuessel, R. (2009) Determination of Cyanuric Acid Residues in Catfish, Trout, Tilapia, Salmon and Shrimp by Liquid Chromatography-Tandem Mass Spectrometry. Analytica Chimica Acta, 637, 201-211.

[6]   Andersen, W.C., Karbiwnyk, C.M., Clark, S.B., Madson, M.R., Gieseker, C.M., Miller, R.A., Rummel, N.G. and Reimschuessel, R. (2008) Determination and Confirmation of Melamine Residues in Catfish, Trout, Tilapia, Salmon, and Shrimp by Liquid Chromatography with Tandem Mass Spectrometry. Journal of Agricultural and Food Chemistry, 56, 4340-4307.

[7]   Litzau, J.J. and Mulligan, K.J. (2007) GC-MS Screen for the Presence of Melamine, Ammeline, Ammelide and Cyanuric Acid.

[8]   Wang, Z.Y., Zhang, L.Y., Yang, W.J., Gong, L.M., He, P.L. and Li, Z. (2010) Screening and Determination of Melamine Residues in Tissue and Body Fluid Samples. Analytica Chimica Acta, 662, 69-75.

[9]   Vallejo-Cordoba, B. and González-Córdova, A.F. (2010) Capillary Electrophoresis for the Analysis of Contaminants in Emerging Food Safety Issues and Food Traceability. Electrophoresis, 31, 2154-2164.

[10]   Tsai, I.-L., Liao, H.-W., Lin, S.-C. and Kuo, C.-H. (2009) Rapid Analysis of Melamine in Infant Formula by Sweeping-Micellar Electrokinetic Chromatography. Journal of Chromatography A, 1216, 8296-8303.

[11]   Klampfl, C.W., Haunschmidt, M., Himmelsbach, M. and Buchberger, W. (2009) Analysis of Melamine in Milk Powder by CZE Using UV Detection and Hyphenation with ESI Quadrupole/TOF MS Detection. Electrophoresis, 30, 1743-1746.

[12]   Yan, N., Zhu, Z.F. and Chen, X.G. (2009) Determination of Melamine in Dairy Products, Fish Feed, and Fish by Capillary Zone Electrophoresis with Diode Array Detection. Journal of Agricultural and Food Chemistry, 57, 807-811.

[13]   Wang, J.Y., Chu, Q.C. and Ye, J.N. (2010) Residue Analysis of Melamine in Milk Products by Micellar Electrokinetic Capillary Chromatography with Amperometric Detection. Food Chemistry, 121, 215-219.

[14]   Ono, S., Inoue, Y., Munechika, T., Yoshimura, T., Morita, H., Rengakuji, S.-I. and Shimasaki, C. (1998) Determination of Melamine Derivatives, Melame, Meleme, Ammeline and Ammelide by High-Performance Cation-Exchange Chromatography. Journal of Chromatography A, 815, 197-214.