FNS  Vol.5 No.6 , March 2014
Microbiological and Nutritional Assessment of Starter-Developed Fermented Tigernut Milk
Abstract: Fermented tigernut milk (FTM) was prepared from three different varieties (fresh yellow, big and small dry brown) of tigernut (Cyperus esculenta) obtained from Bodija market, Ibadan, Oyo state. Fifty two microbial isolates were obtained from FTM at different fermentation times, 32 of which were on MRS agar, 12 on nutrient agar and 8 on malt extract agar. Lactic acid bacteria were identified as Lactobacillus plantarum (LP), Lactococcus lactis (LC), Lactobacillus brevis, Lactococcus cremoris, Lactobacillus bulgaricus and Lactococcus thermophilus (LT). The non-LAB identified includes E. coli, Bacillus species and Proteus species while the yeasts include Saccharomyces cerevisiae and Candida kefyr. Lactic acid bacteria were found to predominate the total microflora of the FTM with their count ranging between 2.0 × 104 cfu/ml to 2.0 × 108 cfu/ml. Microbiological examination revealed that the FTM was safe for consumption as non-LAB counts were below the limit of acceptance which is 2.0 × 105 cfu/ml for dairy milk by Codex Alimentarius Commission. The quantity of lactic acid produced by the LAB isolates ranged between 0.86 g/l - 2.86 g/l while that of hydrogen peroxide ranged between 0.16 g/l - 0.51 g/l. Starter cultures were selected based on predominance of isolate, physiological characteristics, quantity of lactic acid and hydrogen peroxide production. The tiger nut varieties were fermented with the following starter combinations LP, LP/LC, LP/LC/LT, LP/LT while the fifth was spontaneously fermented. The nutritional, chemical and sensory properties of the starter fermented tiger nut milk were evaluated. The highest protein content (24.80%) was obtained in FTM with mixed cultures of LP/LC/LT while the least (3.00%) was obtained in spontaneously fermented milk. There was a significant difference in the FTM varieties. The highest fat content (9.40%) was obtained in spontaneously fermented tiger nut milk while the least (3.40%) was found in FTM with mixed cultures of LP/LC/LT. Fermentation decreased the pH and increased the lactic acid of the starter developed FTM, while sensory evaluation test showed that the FTM with mixed cultures of LP/LC/LT was highly acceptable. In conclusion, an excellent and acceptable FTM can be produced using treatments which comprise of pasteurization at 90° for 15 minutes, fermentation at 45℃ for 18 hours using mixed cultures of Lactobacillus plantarum, Lactococcus lactis and Lactococcus thermophilus.
Cite this paper: Wakil, S. , Ayenuro, O. and Oyinlola, K. (2014) Microbiological and Nutritional Assessment of Starter-Developed Fermented Tigernut Milk. Food and Nutrition Sciences, 5, 495-506. doi: 10.4236/fns.2014.56059.

[1]   Obizoba, I.C. and Anyika, J.U. (1995) Nutritive Value of Baobab Milk (Adansonia digitata), Hungary Rice, Acha (Digitana exillis) Flour. Plant Foods for Human Nutrition, 48, 156-165.

[2]   Singh, T. and Baius, G.S. (1988) Grain Extract-Milk Beverage Processing and Physicochemical Characteristics. Journal of Food Science, 53, 1387-1390.

[3]   Ukwuru, M.U. and Ogbodo, A.C. (2011) Effect of Processing Treatment on the Quality of Tiger Nut Milk. Pakistan Journal of Nutrition, 10, 95-100.

[4]   Hankus, R.W. and Sarret, H.P. (1967) Methods of Comparing Protein Quality of Soybean Infant Formulas in Rat. Journal of Nutrition, 91, 213-216.

[5]   Reddy, N.S., Waghmare, S.Y. and Pande, V. (1990) Formulation and Evaluation of Homemade Weaning Mixes Based on Local Foods. Food Nutrition Bulletin, 12, 138-140.

[6]   Omode, A., Fatoki, O. and Olaogun, K.A. (1995) Physicochemical Properties of Some Underexploited and Non-Conventional Oil Seeds. Journal of Agriculture and Food Chemistry, 11, 50-53.

[7]   Oladele, A.K. and Aina, J.O. (2007) Chemical Composition and Functional Properties of Flour Produced from Two Varieties of Tiger Nut (Cyperus esculenta). African Journal of Biotechnology, 6, 2473-2476.

[8]   Abaejoh, R., Djomdi, I. and Ndojouenkeu, R. (2006) Characteristics of Tiger Nut (Cyprus esculentus) Tubers and Their Performance in the Production of a Milky Drink. Journal of Food Processing and Preservation, 30, 145-163.

[9]   Belewu, M.A. and Belewu, K.Y. (2007) Comparative Physicochemical Evaluation of Tiger Nut, Soy Bean and Coconut Milk Sources. International Journal of Agriculture Biology, 9, 785-787.

[10]   Adejuyitan, J.A., Otunla, E.T., Akande, E.A., Bolarinwa, I.F and Oladokun, F.M. (2009) Some Physicochemical Properties of Flour Obtained from Fermentation of Tiger Nut (Cyperus esculentus) Sourced from a Market in Ogbomoso, Nigeria. African Journal of Food Science, 3, 51-55.

[11]   Borges, O., Gonclaves, B., Sgeoeiro, L., Correeia, P. and Silva, A. (2008) Nutritional Quality of Chestnut Cultivars from Portugal. Food Chemistry, 106, 976-984.

[12]   Onwuka, G.I. (2006) Soaking, Boiling and Antinutritive Factors in Pigeon Pea (Cajanus cajan) and Cowpea (Vigna unguiculata). Journal of Food Processing and Preservation, 30, 616-630.

[13]   Holzapfel, W.H. (2000) Appropriate Starter Culture Technologies for Small Scale Fermentation in Developing Countries. International Journal of Food Microbiology, 5, 19-212.

[14]   Wouters, J.T., Ayad, E.H., Hugenholtz, J. and Smit, G. (2002) Microbes from Raw Milk for Fermented Dairy Products. International Dairy Journal, 12, 91-109.

[15]   Djomdi, R.A.E. and Ndjouenkeu, R. (2006) Characteristics of Tiger Nut (Cyperus-esculentus) Tubers and Their Performance in the Production of a Milky Drink. Journal of food Preservation, 30, 145-163.

[16]   Rita, E.S. (2009) The Use of Tiger Nut (Cyperus esculentus), Cow Milk and Their Composite as Substrate for Yoghurt Production. Pakistan Journal of Nutrition, 6, 755-758.

[17]   Holt, J.G., Kreig, N.G., Peter, H.A., Sneath, S.T. and Williams, S.T. (2000) Bergeys Manual of Systemic Bacteriology 9th Edition, Lippincott Williams and Wilkins Publisher, 175-201,527-528.

[18]   Barnett, J.A., Payne, R.W. and Yarrow, D. (2000) Yeast Characteristics and Identification. Cambridge University Press, London, 100-201.

[19]   A.O.A.C. (2000) Official Methods of Analysis. Association of Official Analytical Chemist, Washington D.C.

[20]   Rodriguez-Tudela, J.L., Chrysanthou, E., Evangelia, P., Juan, M., David, W.D. and Manuel, C.E. (2003) Inter Laboratory Evaluation of Haemocytometer Method of Inoculums Preparations for Testing Antifungal Susceptibility of Filamentous Fungi. Journal of Clinical Microbiology, 41, 5236-523.

[21]   Onovo, J.C. and Ogaraku, A.O. (2007) Studies on Some Microorganisms Associated with Exposed Tigernut (Cyperus esculentus L.) Milk. Journal of Biological Sciences, 7, 1548-1550.

[22]   Adesiyun, A.A. Webb, L. and Rahaman, S. (1995) Microbiological Quality of Raw Cow’s Milk at Collection Centers in Trinidad. Journal of Food Protection, 58, 139-146.

[23]   Kulshrestha, S.B. (1990) Prevalence of Enteropathogenic Serogroups of E. coli in Milk Products Samples from Bareilly and Their Multiple Drug Resistance. Industrial Journal Dairy Science, 43, 373-378.

[24]   Van den Berg, J.C.T. (1988) Dairy Technology in the Tropics and Sub-Topics. Centre for Agricultural Publishing and Documentation, Wegeningen.

[25]   Smoot, L.M. and Pierson, M.D. (1997) Indicator Microorganisms and Microbiological Criteria. In: Doyle, M.P., Beuchat L.R. and Montville T.J., Eds., Food Microbiology: Fundamentals and Frontiers, American Society for Microbiology, Washington DC, 66-80.

[26]   Cogan, T.M. Barbosa, M., Beuvier, E., Bianchi-Salvadori, B., Cocconcelli, P.S., Fernandes, I., Gomez, J., Gomez, R., Kalantzopoulos, G., Ledda, A., Medina, M., Rea, M.C. and Rodriquez, E. (1997) Characterization of the Lactic Acid Bacteria in Artisanal Dairy Products. Journal of Dairy Research, 64, 409-421.

[27]   Holzapfel, W.H. (1997) Use of Starter Cultures in Fermentation on a Household Scale. Food Control, 8, 241-258.

[28]   Spinnler, H.E. and Corrieu, G. (1989) Automatic Method to Quantify Starter Activity Based on pH Measurement. Journal of Dairy Research, 56, 755-764.

[29]   Beal, C. and Corrieu, G. (1994) Viability and Acidification Activity of Pure and Mixed Starters of Streptococcus Salivarius spp. Thermophilu 404 and Lactobacillus delbrueckii spp. bulgaricus 398 at the Different Steps of Their Production. Lebensmittel-Wissenschaft and -Technologie, 27, 86-92.

[30]   Xanthopoulos, V., Petridis, D. and Tzanetakis, N. (2001) Characterization and Classification of Streptococcus thermophiles and Lactobacillus delbrueckii subsp. Bulgaricus Strains Isolated from Traditional Greek Yogurts. Journal of Food Science, 66, 747-752.

[31]   Badis, A., Guetarni, D., Moussa Boudjemaa, B., Henni, D.E. and Kihal, M. (2004) Identification and Technological Properties of Lactic Acid Bacteria Isolated from Raw Goat Milk of Four Algerian Races. Food Microbiology, 21, 579588.

[32]   Buckenhuskes, H.J. (1993) Selection Criteria for Lactic Acid Bacteria to Be Used as Starter Cultures for Various Food Commodities. FEMS Microbiological Review, 12, 253-272.

[33]   Wakil, S.M. and Onilude A.A. (2011) Time Related Total Lactic Acid Bacteria Population Diversity and Dominance in Cowpea-Fortified Fermented Cereal-Weaning Food. African Journal of Biotechnology, 10, 887-895.

[34]   Reed, G. (1982) Prescott and Dunn’s Industrial Microbiolog. 4th Edition, Macmilian Publisher, London, 146-173.

[35]   Paredes-Lopez, O. and Harry, G.I. (1988) Food Biotechnology Review: Traditional Solid-State Fermentations of Plant Raw Materials-Application, Nutritional Significance and Future Prospects. CRC Critical Reviews in Food Science and Nutrition, 27, 159-187.

[36]   Adams, M.R. (1990) Topical Aspects of Fermented Foods. Trends in Food Science and Technology, 1, 141-144.

[37]   Obizola, I.C. and Atii, J.V. (1991) Effect of Soaking, Sprouting, Fermentation and Cooking on Nutrient Composition and Some Anti-Nutritional Factors of Sorghum Seeds. Plants Foods for Human Nutrition, 41, 203-212.

[38]   Aletor, V.A. (1993) Cyanide in Gari II. Assessment of Some Aspect of the Nutrition, Biochemistry and Haematology of the Rats Fed Garri Containing Varying Residual Cyanide Levels. International Journal of Food Science and Nutrition, 44, 289-295.

[39]   Belewu, M.A., Belewu, K.Y. and Bamidele, R.A. (2010) Cyper-Coconut Yoghurt: Preparation, Compositional and Organoleptic Qualities. African Journal of Food Science and Technology, 1, 010-012.

[40]   FAO/WHO (2002) Milk and Milk Products. Joint FAO/WHO Food Standards Programme. CODEX Alimentarius Commossion, 42.

[41]   FAO/WHO (2002) Joint FAO/WHO Expert Report on Diet, Nutrition and the Prevention of Chronic Diseases. Food and Nutrition Bulletin, 24, 255-256.

[42]   Eka, C.U. and Ohaba, J.A. (1977) Microbial Examination of Fulani Milk (Nono) and Butter (Manshanu). Nigeria Journal of Science, 11, 113-122.

[43]   Belewu, M.A. and Abodunrin, O.A. (2006) Preparation of Kunnu from Unexploited Rich Food Source: Tigernut (Cyperus esculentus). World Journal of Diary and Food Sciences, 1, 19-21.

[44]   Ukwuru, M.U., Omachona, L.J. and Onokah, N. (2008) Production and Quality Assessment of Tiger Nut (Cyperus esculentus) Imitation Milk during Storage. Journal of Food Science and Technology, 2, 180-182.

[45]   Koffi, M.E. (1990) Local Packaging of Food in Ghana. Food and Nutrition Bulletin. The United Nations University, Tokyo.