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 FNS  Vol.5 No.6 , March 2014
Occurrence of Escherichia coli O157 in Retailed-Beef and Related Meat Products in Zaria, Nigeria
Abstract: This study evaluated retailed-beef and related meat products for E. coli O157 in Zaria, Nigeria. Sample types included raw meat, “suya” (roasted meat), “balangu” (barbequed meat), “kilishi” (spiced sun dried meat) and “dambu” (shredded fried meat). A total of 182 samples were analyzed for E. coli O157. Isolates were characterized using conventional biochemical methods and Microbact 12E test kit. Susceptibilities of the isolates to 18 commonly used antimicrobial agents were determined by the disk diffusion method. The carriage of stx1 and stx2 genes was determined by PCR. Microbact confirmed 4 E. coli isolates. All isolates exhibited multiple drug resistance to the antimicrobial agents tested. An overall prevalence of 2.2% was obtained for E. coli O157. All 4 isolates of E. coli O157 were isolated from raw meat; two of which harboured the stx1 gene.
Cite this paper: Tafida, S. , Kwaga, J. , Bello, M. , Kabir, J. , Umoh, V. , Yakubu, S. and Nok, A. (2014) Occurrence of Escherichia coli O157 in Retailed-Beef and Related Meat Products in Zaria, Nigeria. Food and Nutrition Sciences, 5, 481-487. doi: 10.4236/fns.2014.56057.
References

[1]   Karch, H., Tarr, P. and Bielazewska, M. (2005) Enterohaemorrhagic Escherichia coli in Human Medicine. International Journal of Medical Microbiology, 295, 405-418.
http://dx.doi.org/10.1016/j.ijmm.2005.06.009

[2]   Nataro, J.P. and Kaper, J.B. (1998) Diarrheagenic Escherichia coli. Clinical Microbiology Reviews, 11, 142-201.

[3]   Paton, J.C. and Paton, A.W. (1998) Pathogenesis and Diagnosis of Shigatoxin Producing Escherichia coli Infections. Clinical Microbiology Reviews, 11, 450-479.

[4]   Coia, J.E. (1998) Clinical, Microbiological and Epidemiological Aspects of Escherichia coli O157 Infection. FEMS Immunology and Medical Microbiology, 20, 1-9. http://dx.doi.org/10.1016/S0928-8244(97)00105-3

[5]   Schmidt, H., Beutin, L. and Karch, H. (1995) Molecular Analysis of the Plasmid-Encoded Hemolysin of Escherichia coli O157:H7 Strain EDL933. Infection and Immunity, 63, 1055-1061.

[6]   Beutin, L., Montenegro, M.A., Orskov, I., Orskov, F., Prada, J., Zimmermann, S. and Stephan, R. (1995) Close Association of Verotoxin (Shiga-Like Toxin) Production with Enterohemolysin Production in Strains of Escherichia coli. Journal of Clinical Microbiology, 27, 2259-2264.

[7]   Armstrong, G.L., Hollingsworth, J. and Morris Jr., J.G. (1996) Emerging Food Borne Pathogens: Escherichia coli O157:H7 as a Model of Entry of a New Pathogen into the Food Supply of the Developing World. Epidemiology Reviews, 18, 29-51.
http://dx.doi.org/10.1093/oxfordjournals.epirev.a017914

[8]   Mortimore, M.J. (1970) Zaria and Its Region. Annals of the Association of American Geographers, 60, 73-80.

[9]   Barrow, G.I. and Feltham, R.K.A. (1995) Cowan and Steel’s Manual for the Identification of Medical Bacteria. Cambridge University Press, 94-163.

[10]   Bauer, A.W., Kirby, W.M., Sherris, J.C. and Turck, M. (1966) Antibiotic Susceptibility Testing by a Standardized Single Disk Method. American Journal of Clinical Pathology, 45, 493-496.

[11]   Clinical Laboratory Standards Institute (CLSI) (2006) Performance Standards for Antimicrobial Susceptibility Testing. Sixth Informational Supplement CLSI Document M100-516, Wayne.

[12]   Al-Gallas, N., Ben Aissa, R., Attia Annabi, Th., Bahri, O. and Boudabous, A. (2002) Isolation and Characterization of Shiga Toxin-Producing Escherichia coli from Meat and Dairy Products. Food Microbiology, 19, 389-398.
http://dx.doi.org/10.1006/fmic.2002.0488

[13]   Abd El-Atty, N.S. and Meshref, A.M.S. (2007) Prevalence of Salmonella and E. coli O157 in Some Foods. Beni-Suef Vet. Medicine Journal, 5th. Scientific Conference, 73-78.

[14]   Enabulele, S. and Uraih, N. (2009) Enterohaemorrhagic Escherichia coli O157:H7. Prevalence in Meat and Vegetables sold in Benin City, Nigeria. African Journal of Microbiological Research, 5, 276-279.

[15]   Magwira, C.A., Gashe, B.A. and Collison, E.K. (2005) Prevalence and Antibiotic Resistance Profiles of Escherichia coli O157:H7 in Beef Products from Retail Outlets in Gaborone, Botswana. Journal of Food Protection, 68, 403-406.

[16]   Catry, B., Laevens, H., Devriese, L.A., Opsomer, G. and De Kruif, A. (2003) Antimicrobial Resistance in Livestock. Journal of Veterinary Pharmacology and Therapeutics, 26, 81-93.
http://dx.doi.org/10.1046/j.1365-2885.2003.00463.x

[17]   World Health Organization (WHO) (2000) WHO Global Principles for the Containment of Antimicrobial Resistance in Animals Intended for Food. Report of a WHO Consultation, WHO Department of Communicable Disease Surveillance and Response, Geneva, 1-7.

[18]   Threlfall, E.J., Angulo, F.J. and Wall, P.G. (1998) Ciprofloxacin-Resistant Salmonella Typhimurium DT104. Veterinary Record, 142, 255.

[19]   Fluit, A.C., Jones, M.E., Schmitz, F.J., Acar, J., Gupta, R. and Verhoef, J. (2000) For the SENTRY Participants Group. Bacteremia in European Hospitals, Incidence and Antimicrobial Susceptibility. Clinical Infectious Diseases, 30, 454- 460. http://dx.doi.org/10.1086/313710

[20]   Fluit, A.C. and Schmitz, F.J. (1999) Class 1 Integrons, Gene Cassettes, Mobility and Epidemiology. European Journal of Clinical Microbiology and Infectious Diseases, 18, 761-770. http://dx.doi.org/10.1007/s100960050398

[21]   Schmidt, H., Geitz, C., Phillips, I.T., Matthias, F. and Karch, H. (1999) Non-O157 Pathogenic Shiga Toxin Producing Escherichia coli: Phenotypic and Genetic Profiling of Virulence Traits and Evidence for Clonality. Journal of Infectious Diseases, 179, 115-123. http://dx.doi.org/10.1086/314537

[22]   Pradel, N., Boukhors, K., Bertin, Y., Forestier, C., Martin, C. and Livrelli, V. (2001) Heterogeneity of Shiga Toxin Producing Escherichia coli Strains Isolated from Hemolytic-Uremic Syndrome Patients, Cattle and Food Samples in Central France. Applied and Environmental Microbiology, 67, 2460-2468.
http://dx.doi.org/10.1128/AEM.67.6.2460-2468.2001

[23]   Adwan, M.G. and Adwan, M.K. (2004) Isolation of Shiga Toxigenic Escherichia coli from Raw Beef in Palestine. International Journal of Food Microbiology, 97, 81-84.
http://dx.doi.org/10.1016/j.ijfoodmicro.2004.03.032

[24]   Karch, H., Meyer, T., Rüssmann, H. and Heesemann, J. (1992) Frequent Loss of Shiga-Like Toxin Genes in Clinical Isolates of Escherichia coli upon Subcultivation. Infection and Immunity, 60, 3464-3467.

[25]   Arthur, T.M., Barkocy-Gallagher, G.A., Rivera-Betancourt, M. and Koohmaraie, M. (2002) Prevalence and Characterization of Non-O157 Shiga Toxin-Producing Escherichia coli on Carcasses in Commercial Beef Cattle Processing Plants. Applied and Environmental Microbiology, 68, 4847-4852.
http://dx.doi.org/10.1128/AEM.68.10.4847-4852.2002

[26]   Cortez, A.L.L., Carvalho, C.F.B., Ikuno, A.A., Bu¨rger, K.P. and Vidal-Martins, A.M.C. (2006) Identification of Salmonella spp. Isolates from Chicken Abattoirs by Multiplex-PCR. Research in Veteterinary Science, 81, 40-344.
http://dx.doi.org/10.1016/j.rvsc.2006.03.006

[27]   Bello, M., Lawan, M.K., Kwaga, J.K.P. and Raji, M.A. (2011) Assessment of Carcass Contamination with E. coli O157 before and after Washing with Water at Abattoirs in Nigeria. International Journal Food Microbiology, 150, 184-186. http://dx.doi.org/10.1016/j.ijfoodmicro.2011.07.029

 
 
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