AID  Vol.4 No.1 , March 2014
GenoType MTBDRplus as a Complementary Tool for the Typing of Mycobacterium tuberculosis
Abstract: The aim of this study was to investigate the usefulness of combining profiles obtained by using a line probe assay (LPA) originally intended to characterize the resistance of two major anti-tuberculosis drugs to the association of spoligotyping and MIRU-VNTR, in order to improve its discriminatory power. For this purpose, 74 strains of Mycobacterium tuberculosis belonging to the same cluster after spoligotyping were further typed by using the 24 loci MIRU/VNTR. These strains were then tested by the GenoType MTBDRplus, and profiles obtained were analyzed within previously obtained clusters. The combination of spoligotying and MIRU-VNTR led to the consolidation of 56 of them (75.7%) in 9 clusters. Most of the strains (54, 96.4%) were multidrug resistant (MDR). From the 9 initial clusters, the addition of GenoType MTBDRplus helped to define 26 profiles including 11 unique profiles, and 3 original clusters remained undifferentiated. Results obtained express the relevance of combining this method which improved quite significantly the discriminatory power in typing Mycobacterium tuberculosis.
Cite this paper: Ouassa, T. , Loukou, G. and Faye-Kette, H. (2014) GenoType MTBDRplus as a Complementary Tool for the Typing of Mycobacterium tuberculosis. Advances in Infectious Diseases, 4, 26-29. doi: 10.4236/aid.2014.41005.

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