OJCD  Vol.4 No.1 , March 2014
Evaluation of Some Etiological Factors of Haemolytic Disease of the New Born in Ile-Ife
Abstract: Heamolytic disease of the newborn is a neonatal disease associated with red cells heamolysis resulting from red cell antigens of the foetus and mother red cells antibodies. This study involved a cross sectional laboratory based analytical survey, eighty neonates with pyrexia of unknown origin and twenty neonates apparently healthy were selectected for this study. ABO and Rh blood group, Direct Coombs’ test, Glucose-6-phosphate dehydrogenase estimation and blood culture were performed on blood samples collected from each neonate. Blood group O had the highest number of neonates (60% control; 61% test); 80% and 81.3% of the control and test neonates are Rh positive respectively. None of the control subjects are positive to DCT while 5% of test subjects positive to DCT; 4% and 8.7% of the control and test respectively are positive to G6-PD deficiency. The study recorded 0% growth in blood culture of control subjects while 2.5% of the neonates blood samples yielded when cultured. Though prevalence rate of HDN in our society is diminishing gradually based on this study, there is need to look inwardly to other aetiological factors that can cause HDN apart from blood group incompatibility, since sudden infant death is on increase now.

Cite this paper: Fadairo, J. , Aladenika, S. , Osaiyuwu, C. , Olaniyan, M. and Aghatise, K. (2014) Evaluation of Some Etiological Factors of Haemolytic Disease of the New Born in Ile-Ife. Open Journal of Clinical Diagnostics, 4, 5-11. doi: 10.4236/ojcd.2014.41002.

[1]   Van Dijk, B.A., Hirasing, R.A. and Overbeeke, M.A. (1999) Hemolytic Disease of the Newborn and Irregular Blood Group Antibodies in the Netherlands: Prevalence and Morbidity. Ned Tijdschr Geneeskd, 143, 1465-1469.

[2]   Chavez, G.F., Mulinare, J. and Edmonds, L.D. (1991) Epidemiology of Rh Hemolytic Disease of the Newborn in the United States. The Journal of the American Medical Association, 265, 3270-3274.

[3]   Dacie, J.V. and Lewis, S.M. (2001) Practical Haematology. In: Lewis, S.M., Bain, B.J. and Bates, I. Eds., Practical Heamatology, 9th Edition, Churchill Livingstone, Harcourt Publishers Limited, London, 444-451.

[4]   Cheesbrough, M. (2000) Direct Combs’ Test. In: District Laboratory Practice in Tropical Countries, Part 2. 2nd Editon, Cambridge University Press, New York, 221-245.

[5]   Thakral, B., Malhotra, S., Saluja, K., Kumar, P. and Marwaha, N. (2010) Hemolytic Disease of Newborn Due to Anti-Jkb in a Woman with High Risk Pregnancy. Transfusion and Apheresis Science, 43, 41-43.

[6]   Luchtman-Jones, L., Schwartz, A.L. and Wilson, D.B. (1997) The Blood and Hematopoietic System. In: Fanaroff, A.A. and Martin, R.J., Eds., Neonatal-Perinatal Medicine-Diseases of the Fetus and Infant, 6th Edition, Mosby, St. Louis, 1201-1287.

[7]   Olaniyan, T.O., Ajibola, B.M., Rasong, H., Dare, B.J. and Shafe, M.O. (2013) Blood Group and Rhesus Factor Pattern among Indigenes of FCT, Abuja, Nigeria. Journal of Community Medicine & Health Education, 3, 208.

[8]   Akinnuga, A.M., Bamidele, O., Amosu, A.M. and Ugwah, G.U. ( 2011) Distribution of ABO and Rh Blood Groups among Major Ethnic Groups of Medical Students of Madonna University Teaching Hospital, Elele, Nigeria. Asian Journal of Medical Sciences, 3, 106-109.

[9]   Adeyemo, O.A. and Soboyejo, O.B. (2006) Frequency Distribution of ABO, RH Blood Groups and Blood Genotypes among the Cell Biology and Genetics Students of University of Lagos, Nigeria. African Journal of Biotechnology, 5, 2062-2065.

[10]   Nwauche, C.A. and Ejele, O.A. (2004) ABO and Rhesus Antigens in a Cosmopolitan Nigeria Population. Nigerian Journal of Medicine, 13, 263-266.

[11]   Kulkarni, A.G., Peter, B., Ibazebo, R., Dash, B. and Fleming, A.F. (1985) The ABO and Rhesus Groups in the North of Nigeria. Annals of Tropical Medicine and Parasitology, 79, 83-88.

[12]   Falusi, A.G., Ademowo, O.G., Latunji, C.A., Okeke, A.C., Olatunji, P.O., Onyekwere, T.O., Jimmy, E.O., Raji, Y., Hedo, C.C., Otukonyong E.E. and Itata, E.O. (2000) Distribution of ABO and RH Genes in Nigeria. African Journal of Medicine and Medical Sciences, 29, 23-26.

[13]   Avent, N.D. and Reid M.E. (2000) The Rh Blood Group System: A Review. Blood, 95, 375-387.

[14]   Seeley, R.R., Stephens, T.D. and Tate, P. (1998) Anatomy and Physiology. 4th Edition, the McGraw Hill Companies, Inc., New York City, 1098.

[15]   Dean, L. (2005) Blood Groups and Red Cell Antigens [Internet]. National Center for Biotechnology Information (US), Bethesda (MD), Chapter 4, Hemolytic Disease of the Newborn.

[16]   Heddle, N.M., Wentworth, P., Anderson, D.R., Emmerson, D., Kelton, J.G. and Blajchman, M.A. (1995) Three Examples of Rh Haemolytic Disease of the Newborn with a Negative Direct Antiglobulin Test. Transfusion Medicine, 5, 113-116.

[17]   Mehta, A., Mason, P.J. and Vulliamy, T.J. (2000). Glucose-6-Phosphate Dehydrogenase Deficiency. Best Practice & Research Clinical Haematology, 13, 21-38.

[18]   Beutler, E. (1994) G6PDdeficiency. Blood, 84, 3613-3636.

[19]   Manik, M., Asok, K.D, Syamali, M. and Pradip, K.D. (2012) Study of Glucose-6-Phosphate Dehydrogenase Defi- ciency in Neonatal Jaundice. Journal of Pharmacy and Biological Sciences, 1, 30-36.

[20]   WHO (1997) Technical Report on Standardization of Procedures for the Study of G-6-PD.

[21]   Asindi, A.A., Ibia, E.O. and Udo, J.J. (1991) Mortality Pattern among Nigerian Children in the 1980s. The Journal of Tropical Medicine and Hygiene, 94, 152-155.

[22]   Komolafe, A.O. and Adegoke, A.A. (2008) Incidence of Bacterial Septicaemia in Ile-Ife Metropolis, Nigeria Malaysian Journal of Microbiology, 4, 51-61.

[23]   Nwadioha, S.I., Nwokedi, E., Odimayo, M.S., Okwori, E.E. and Kashibu, E. (2010) Bacterial Isolates in Blood Cultures of Children with Suspected Septicaemia in a Nigerian Tertiary Hospital. The Internet Journal of Infectious Diseases, 8.

[24]   West, B.A. and Peterside, O. (2012) Sensitivity Pattern among Bacterial Isolates in Neonatal Septicaemia in Port Harcourt. Annals of Clinical Microbiology and Antimicrobials, 11, 7.

[25]   Desai, K.J. and Malek, S.S. (2010) Neonatal Septicemia: Bacterial Isolates & Their Antibiotics Susceptibility Patterns. National Journal of Integrated Research in Medicine, 1, 12-15.

[26]   Iregbu, K.C., Elegba, O.Y. and Babaniyi, I.B. (2006) Bacteriological Profile of Neonatal Septicaemia in a Tertiary Hospital in Nigeria. African Health Sciences, 6, 151.

[27]   Onyearugha, C.N., Onyire, B.N. and Ugboma, H.A.A. (2011) Neonatal Jaundice: Prevalence and Associated Factors as Seen in Federal Medical Centre Abakaliki, Southeast Nigeria. Journal of Clinical Medicine and Research, 3, 40-45.

[28]   Farhud, D.D. and Yazdanpanah, L. (2008) Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency Iranian. Journal of Public Health, 37, 1-18.