JBiSE  Vol.7 No.3 , February 2014
Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids
ABSTRACT

Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation for precipitation of bacterial pellets, and subsequent lysis of the pellets. This process is time-consuming and laborious, especially when the sample number is high. Here, we describe a more convenient method for MiniPrep analysis that utilizes solid medium-based cultivation of bacteria.


Cite this paper
Sato, M. , Inada, E. , Saitoh, I. and Matsumoto, Y. (2014) Microbial and enzyme technology: An efficient and convenient method for MiniPrep analysis of recombinant plasmids. Journal of Biomedical Science and Engineering, 7, 105-107. doi: 10.4236/jbise.2014.73013.
References
[1]   Sambrook, J., Fritsch, E.F. and Maniatis, T. (1989) Molecular cloning: A laboratory manual. 2nd Edition, Cold Spring Harbor Laboratory Press, Cold Spring Harbor.

[2]   DNA Purification in Promega Home Page.
(http://www.promega.com/resources/product-guides-and-selectors/protocols-and-applications-guide/dna-purification/

[3]   QIAGEN Plasmid Purification System in Qiagen Home Page. http://www.ebiotrade.com/buyf/productsf/qiagen/QIAGEN_plasmid_purification_system.htm

[4]   Sato, M., Akasaka, E., Saitoh, I., Ohtsuka, M., Nakamura, S., Sakurai, T. and Watanabe, S. (2012) A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates. Journal of Biomedical Science and Engineering, 5, 406-408.
http://dx.doi.org/10.4236/jbise.2012.57051

[5]   DNA Plasmid Miniprep Protocol.
http://www.protocol-online.org/cgi-bin/prot/view_cache.cgi?ID=3062

[6]   Mini-prep Protocol.
http://irc.igd.cornell.edu/Protocols/Mini-prep.htm

 
 
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