Minipreparation (MiniPrep) analysis is an essential step for obtaining a recombinant plasmid that carries a DNA insert containing a gene of interest. The most commonly used method for this involves cultivation of transformed Escherichia coli (E. coli) in liquid medium, brief centrifugation for precipitation of bacterial pellets, and subsequent lysis of the pellets. This process is time-consuming and laborious, especially when the sample number is high. Here, we describe a more convenient method for MiniPrep analysis that utilizes solid medium-based cultivation of bacteria.
 DNA Purification in Promega Home Page.
 QIAGEN Plasmid Purification System in Qiagen Home Page. http://www.ebiotrade.com/buyf/productsf/qiagen/QIAGEN_plasmid_purification_system.htm
 Sato, M., Akasaka, E., Saitoh, I., Ohtsuka, M., Nakamura, S., Sakurai, T. and Watanabe, S. (2012) A simplified protocol for the semi-large scale recovery of plasmids from Escherichia coli grown on agar plates. Journal of Biomedical Science and Engineering, 5, 406-408.
 DNA Plasmid Miniprep Protocol.
 Mini-prep Protocol.