Health  Vol.6 No.4 , February 2014
A study on the transmission of helicobacter pylori from food prepared and consumed under hygienic and unhygienic conditions: A first study using biopsy samples

Objective: A comparative analysis on transmission of Helicobacter pylori in people who consumed food prepared in both hygienic and unhygienic conditions. Method: The study was carried out in 1000 people across India. The Ratio of Male: female was 700: 300 with age group ranging from 20 - 39. Three gastric biopsies were collected for identification of Helicobacter pylori. PCR (Polymerase Chain Reaction) amplification was done by using 16srRNA primers. Result: The analysis showed the prevalence of H. pylori as 70.8% in individuals frequently consuming food prepared under less hygienic condition (street vendor’s food) whereas 60% (less prevalence) in individuals consuming food prepared under hygienic conditions. Conclusion: The results showed that most of the food that we consume daily is not involved much in the transmission of Helicobacter pylori. The study also showed that the street vendor’s food is not directly involved in the transmission of this bacterium. The unhygienic preparation of food and poor personal hygiene play a vital role in the transmission of these bacteria.

Cite this paper: Ahmed, K. , Madompoyil, B. , Ahi, J. , Khan, A. , Tiwari, S. and Habeeb, M. (2014) A study on the transmission of helicobacter pylori from food prepared and consumed under hygienic and unhygienic conditions: A first study using biopsy samples. Health, 6, 274-283. doi: 10.4236/health.2014.64040.

[1]   Warren, J.R. and Marshall, B.J. (1983) Unidentified curved bacilli on gastric epithelium in active chronic gastritis. Lancet, l, 1273-1275.

[2]   Correa, P., Mufioz, N., Cuello, C., Fox, J., Zavala, D. and Ruiz, B. (1989) The role of Campylobacter pylori in gastro-duodenal disease. In: Fenoglio-Preiser, C., Ed., Progress in Surgical Pathology, Field & Wood, Philadelphia, 191-210.

[3]   Rugge, M., DiMario, F., Cassaro, M., Baffa, R., Farinati, F., Rubio, J., et al. (1993) Pathology of the gastric antrurn and body associated with Helicobacter pylori infection in non-ulcerous patients: Is the bacterium a promoter of intestinal metaplasia? Histopathology, 22, 9-15.

[4]   Kuipers, F.J., Thijs, J.C. and Festen, H.P.M. (1995) The prevalence of Helicobacter pylori infection in peptic ulcer disease. Alimentary Pharmacology & Therapeutics, 9, 59-69.

[5]   Tytgat, G.N.J., Noach, L.A. and Rauws, E.A.J. (1993) Helicobacter pylori infection and duodenal ulcer disease. Gastroenterology Clinics of North America, 22, 127-140.

[6]   Forman, D. (1995) The prevalence of Helicobacter pylori infection in gastric cancer. Alimentary Pharmacology & Therapeutics, 9, 53-58.

[7]   The EUROGAST Study Group. (1993) An international association between Helicobacter pylori infection and gastric cancer. Lancet, 341, 1359-1362.

[8]   Parsonnet, J., Hansen, S., Rodriguez, L., Gelb, A.B., Warnke, R.A., Jellum, E., et al. (1994) Helicobacter pylori infection and gastric lymphoma. The New England Journal of Medicine, 330, 1267-1271.

[9]   Buiatti, E., Mufioz, N., Vivas, J., Cano, E., Peraza, S., Carillo, E., et al. (1994) Difficulty in eradicating Helicobacter pylori in a population at high risk for stomach cancer in Venezuela. Cancer Causes Control, 5, 249-254.

[10]   Parsonnet, J. (1995) The incidence of Helicobacter pylori infection. Alimentary Pharmacology & Therapeutics, 9, 45-51.

[11]   Glupczynski, Y. and Burette, A. (1990) Drug therapy for Helicobacter pylori infection: problems and pitfalls. The American Journal of Gastroenterology, 85, 1545-1551.

[12]   Coelho, L.G., Passos, M.C., Chausson, Y., Costa, E.L., Maia, A.F., Brandao, M.J., et al. (1992) Duodenal ulcer and eradication of Helicobacter pylori in a developing country. An 18-month follow-up study. Scandinavian Journal of Gastroenterology, 27, 362-366.

[13]   Goodman, K.J. and Correa, P. (1995) The transmission of Helicobacter pylori. A critical review of the evidence. International Journal of Epidemiology, 24, 875-887.

[14]   Sobala, G.M., Crabtree, J., Dixon, M.F., Schorah, C.J., Taylor, J.D., Rathbone, B.J., et al. (1991) Acute Helicobacter pylori infection: Clinical features, local and systemic immune response, gastric mucosal histology, and gastric juice ascorbic acid concentrations. Gut, 32, 1415-1418.

[15]   Megraud, F. (1995) Transmission of Helicobacter pylori: faecal-oral versus oral-oral. Alimentary Pharmacology & Therapeutics, 9, 85-91.

[16]   Neale, K.R. and Logan, R.P. (1995) The epidemiology and transmission of Helicobacter pylori infection in children. Alimentary Pharmacology & Therapeutics, 9, 77-84.

[17]   Fox, J.G., Blanco, M.C., Yan, L., Shames, B., Polidoro, D., Dewhirst, F.E., et al. (1993) Role of gastric pH in isolation of Helicobacter mustelae from the feces of ferrets. Gastroenterology, 104, 86-92.

[18]   Fox, J.G., Paster, B.J., Dewhirst, F.E., Taylor, N.S., Yan, L.L., Macuch, P.J., et al. (1992) Helicobacter mustelae isolation from feces of ferrets: evidence to support fecal-oral transmission of a gastric helicobacter. Infection and Immunity, 60, 606-611.

[19]   Thomas, J.E., Gibson, G.R., Darboe, M.K., Dale, A. and Weaver, L.T. (1992) Isolation of Helicobacter pylori from human faeces. Lancet, 340, 1194-1195.

[20]   Lee, A., Fox, J.G., Otto, G., Dick, E.H. and Krakowka, S. (1991) Transmission of Helicobacter spp. A challenge to the dogma of fecal-oral spread. Epidemiology and Infection, 107, 99-109.

[21]   Blaser, M.J. (1987) Gastric Campylobacter-like organisms, gastritis and peptic ulcer disease. Gastroenterology, 93, 371-383.

[22]   Shahamat, M., Paszco-Kolva, C., Yamamoto, H. and Colwell, R.R. (1989) Ecological studies of Campylobacter pylori. (Abstract). Klinische Wochenschrift, 67, 62-63.

[23]   Shahamat, M., Vives-Rego, J., Paszco-Kolva, C., Pearson, A.D. and Colwell, R.R. (1989) Survival of Campylobacter pylori in river water. (Abstract). Klinische Wochenschrift, 67, 63.

[24]   West, A.P., Millar, M.R. and Tompkins, D.S. (1992) Effect of physical environment on survival of Helicobacter pylori. Journal of Clinical Pathology, 45, 228-231.

[25]   Klein, P.D., Graham, D.Y., Gaillour, A., Opekun, A.R. and Smith, E.O. (1991) Water source as a risk factor for Helicobacter pylori infection in Peruvian children. Gastrointestinal Physiology Working Group. Lancet, 337, 1503-1506.

[26]   Hopkins, R.J., Vial, P.A., Ferreccio, C., Ovalle, J., Prado, P., Sotomayor, V., et al. (1993) Seroprevalence of Helicobacter pylori in Chile: Vegetables may serve as one route of transmission. The Journal of Infectious Diseases, 168, 222-226.

[27]   Begue, R.E., Gonzales, J.L., Correa-Gracian, H. and Tang, S.C. (1998) Dietary Factors Associated with the Transmission of Helicobacter Pylori in Lima Peru. The American Journal of Tropical Medicine and Hygiene, 59, 637-640.

[28]   Chakravarty, I. and Canet, C. (1996) Street foods in Calcutta. Food. Nutrition and Agriculture, Publication no17/ 18, 30-37.

[29]   Ahmed, K.S, Khan, A.A, Tiwari, S.K., Habeeb, A., Ahmed, I., Shaik, M.A., et al. (2006) Prevalence study to elucidate the transmission pathways of Helicobacter pylori at oral and gastroduodenal sites of a South Indian population. Singapore Medical Journal, 47, 291-296.

[30]   Ahmed, K.S, Khan, A.A, Ahmed, I., Tiwari, S.K., Habeeb, A., Ahi, J.D., et al. (2007) Impact of household hygiene and water source on the prevalence and transmission of Helicobacter pylori: A South Indian perspective. Singapore Medical Journal, 48, 543-549.

[31]   Hollingshead, A.B. (1957) Two factor index of social position. Yale University Press, New Haven.

[32]   Jiang, X. and Doyle, M.P. (1998) Effect of environmental and substrate factors on survival and growth of Helicobacter pylori. Journal of Food Protection, 61, 929-933.

[33]   FDA 2001 Food Code and Wisconsin Food Code: 3-401.11, Raw Animal Foods; 3-401.12, Microwave Cooking; 3-401.13, Plant Food Cooking for Hot Holding.

[34]   Shigeyoshi, I., Masakazu, K., Yoshio, Y., Toshiro, Y., Atsushi, I., Hideyuki, K., et al. (2003) Vector Potential of Cockroaches for Helicobacter Pylori Infection. The American Journal of Gastroenterology, 98, 1500-1503.

[35]   Stevenson, T.H., Bauer, N., Lucia, L.M. and Acuff, G.R. (2000) Attempts to isolate Helicobacter from cattle and survival of Helicobacter pylori in beef products. Journal of Food Protection, 63, 174-178.

[36]   Fujimura, S., Kawamura, T., Kato, S., Tateno, H. and Watanabe, A. (2002) Detection of Helicobacter pylori in cow’s milk. Letters in Applied Microbiology, 35, 504-507.

[37]   Smith, P.W. (1981) “Milk Pasteurization” fact sheet number 57. US. Department of Agriculture Research Service, Washington DC.

[38]   Quaglia, N.C., Dambrosio, A., Normanno, G., Parisi, A., Firinu, A., Lorusso, V. and Celanoa, G.V. (2007) Survival of Helicobacter pylori in artificially contaminated ultra-high temperature and pasteurized milk. Food Microbiology, 24, 296-300.

[39]   Grubel, P., Hoffman, J.S., Chong, F.K., Burstein, N.A., Mepani, C. and Cave, D.R. (1997) Vector potential of houseflies (Musca domestica) for Helicobacter pylori. Journal of Clinical Microbiology, 35, 1300-1303.

[40]   Centers for Disease Control and Prevention. (1997) Health hints for the traveler. Health information for international travel 1996-97. CDC, U.S. Department of Health and Human Services, Public Health Service, Atlanta, 177-196.

[41]   Goodman, K.J., Correa, P., Tengana, A.H., Delany, J.P. and Collazos, T. (1997) Nutritional factors and Helicobacter pylori infection in Colombian children. Journal of Pediatric Gastroenterology & Nutrition, 25, 507-515.

[42]   Jarosz, M., Dzieniszewski, J., Dabrowska-Ufniarz, E., Wartanowicz, M., Ziemlanski, S. and Reed, P.I. (1998) Effects of high dose vitamin C treatment on Helicobacter pylori infection and total vitamin C concentration in gastric juice. European Journal of Cancer Prevention, 7, 449-454.

[43]   Grubel, P., Huang, L., Masubuchi, N., Stutzenberger, F.J. and Cave, D.R. (1998) Detection of Helicobacter pylori DNA in houseflies (Musca domestica) on three continents. (Letter). Lancet, 352, 788-789.