Health  Vol.6 No.4 , February 2014
The relationship between Helicobacter pylori disease and bacterial count in stomach

Several studies showed significant relationships between bacterial counts and the severity and type of disease in patients. The aim of this study was to evaluate the relationship between Helicobacter pylori disease and bacterial count. In this study, 287 patients with dyspeptic symptoms were evaluated. Three variables including patient-reported data, clinical signs and bacterial load of gastric specimens were analyzed. Biopsy samples were collected from patients who were referred for endoscopies because of dyspeptic symptoms. Initially, the clinical status was evaluated and recorded by a questionnaire. DNA extraction was performed and H. pylori was analyzed for bacterial count by Real-time PCR assay and specific primers and probe. The variety range of bacteria in specimens was 104 to 1012. The results revealed that a greater relationships existed between 1012 and gastric cancer (p = 0.036), also 104 and acid reflux (p = 0.006) and vomiting (p = 0.047). Real-time PCR assay provides a highly accurate, rapid and precise method for detection of H. pylori and determination of progressive disease due to this bacterium.

Cite this paper: Ghorbani-Dalini, S. , Kargar, M. , Doosti, A. and Najafi, A. (2014) The relationship between Helicobacter pylori disease and bacterial count in stomach. Health, 6, 259-262. doi: 10.4236/health.2014.64038.

[1]   Kargar, M., Baghernejad, M., Doosti, A. and Ghorbani-Dalini, S. (2011) Clarithromycin resistance and 23S rRNA mutations in Helicobacter pylori isolates in Iran. African Journal of Microbiology Research, 5, 853-856.

[2]   Godoy, A.P.O., Ribeiro, M.L., Benvengo, Y.H.B., Vitiello, L., Miranda, M.C.B., Mendonca, S. and Pedrazzoli, J., Jr. (2003) Analysis of antimicrobial susceptibility and virulence factors in Helicobacter pylori clinical isolates. BMC Gastroenterology, 3, 20,

[3]   Wu, C.C., Chou, P.Y., Hu, C.T., Liu, Z.C., Lin, C.Y., Tseng, Y.H. and Lin, N.T. (2005) Clinical relevance of the vacA, iceA, cagA, and flaA genes of Helicobacter pylori strains in eastern Taiwan. Journal of Clinical Microbiology, 43, 2913-2915.

[4]   Kusters, J.G., Van Vliet, A.H.M. and Kuipers, E.J. (2006) Pathogenesis of Helicobacter pylori Infection. Clinical Microbiology Reviews, 19, 449-490.

[5]   Kargar, M., Souod, N., Doosti, A. and Ghorbani-Dalini, S. (2011) Prevalence of Helicobacter pylori vacuolating cytotoxin A gene as a predictive marker for different gastrodoudenal diseases. Iranian Journal of Clinical Infectious Diseases, 6, 85-89.

[6]   Kargar, M., Baghernejad, M., Doosti, A. and Ghorbani-Dalini, S. (2011) Clarithromycin resistance and 23S rRNA mutations in Helicobacter pylori. In: Croatia, O.P., Ed., Gasrtointestinal Endoscopy, InTech, 99-124.

[7]   Kargar, M., Souod, N., Ghorbani-Dalini, S., Doosti, A. and Rezaeian, A.A. (2011) Evaluation of cagA tyrosine phosphorylation DNA motifs in Helicobacter pylori isolates from gastric disorder patients in West of Iran. Scientific Research and Essays, 6, 6454-6458.

[8]   Kargar, M., Ghorbani-Dalini, S., Doosti, A. and Baghernejad, M. (2011) Molecular assessment of clarithromycin resistant Helicobacter pylori strains using rapid and accurate PCR-RFLP method in gastric specimens in Iran. African Journal of Biotechnology, 10, 7675-7678.

[9]   Kargar, M., Ghorbani-Dalini, S., Doosti, A. and Souod, N. (2012) Real-time PCR for Helicobacter pylori quantification and detection of clarithromycin resistance in gastric tissue from patients with gastrointestinal disorders. Research in Microbiology, 163, 109-113.

[10]   Kargar, M., Doosti, A. and Ghorbani-Dalini, S. (2012) Detection of four clarithromycin resistance point mutations in Helicobacter pylori: Comparison of real-time PCR and PCR-RFLP methods. Comparative Clinical Pathology, 22, 1007-1013.

[11]   Lascols, C., Lamarque, D., Costa, J.M., Copie-Bergman, C., Glaunec, J.M.L., Deforges, L., Soussy, C.J., Petit, J.C., Delchier, J.C. and Tankovic, J. (2003) Fast and accurate quantitative detection of Helicobacter pylori and identification of clarithromycin resistance mutations in H. pylori isolates from gastric biopsy specimens by Real-Time PCR. Journal of Clinical Microbiology, 41, 4573-4577.

[12]   Wain, J., Bay, P.V.B., Vinh, H., Duong, N.M., Diep, T.S., Walish, A.L., Parry, C.M., Hasserjian, R.P., HoHo, V.A., Tran, T.H., Farrar, J., White, N.J. and Day, N.P. (2001) Quantitation of bacteria in bone marrow fromp with typhoid fever: Relationship between counts and clinical features. Journal of Clinical Microbiology, 39, 1571-1576.

[13]   Sonthayanon, P., Chierakul, W., Wuthiekanun, V., Phimda, K., Pukrittayakamee, S., Day, N.P. and Peacock, S.J. (2009) Association of high Orientia tsutsugamushi DNA loads with disease of greater severity in adults with scrub typhus. Journal of Clinical Microbiology, 47, 430-434.

[14]   Palaci, M., Dietze, R., Hadad, D.J., Ribeiro, F.K.C., Peres, R.L., Vinhas, S.A., Maciel, E.L., do Valle Dettoni, V., Horter, L., Boom, W.H., Johnson, J.L. and Eisenach, K.D. (2007) Cavitary disease and quantitative sputum bacillary load in cases of pulmonary tuberculosis. Journal of Clinical Microbiology, 45, 4064-4066.