OJAB  Vol.3 No.1 , February 2014
Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions
Abstract: Maize tassel-multiwalled carbon nanotube (MT-MWCNT) composite has been used as a matrix for physical adsorption of horseradish peroxidase (HRP) onto the surface of a glassy carbon electrode through electrostatic interactions. The HRP/MT-MWCNT biosensor was applied for the detection of Zn2+ in aqueous solution. The biosensor designed was able to determine Zn2+ in the range of 0.35 - 12 mg/L with a detection limit of 7.5 μg/L. The inhibition was found to be reversible and uncompetitive when data were modeled using the Dixon and Cornish-Bowden plots. The biosensor was found to have good repeatability, reproducibility and high selectivity. The developed biosensor can be used to detect other HRP inhibiting trace metal ions.
Cite this paper: Moyo, M. (2014) Horseradish Peroxidase Biosensor to Detect Zinc Ions in Aqueous Solutions. Open Journal of Applied Biosensor, 3, 1-7. doi: 10.4236/ojab.2014.31001.

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