Health  Vol.3 No.3 , March 2011
Effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells
ABSTRACT
In the present study, we investigated the effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells. Collagens were extracted from bluefin tuna (Thunnus orientails) abdominal, mackerel, and carp skin. The calf and salmon collagen were used reagent grade as a standard samples. The main protein band pattern produced by SDS-PAGE of all collagen samples consisted of two chains and one chain. For collagen peptides samples, bluefin tuna ab-dominal skin collagen and salmon skin collagen were hydrolyzed by trypsin. Among samples, salmon, mackerel, carp collagen, and their collagen peptides did not significantly reduce relative cell growth. However, the bluefin tuna abdominal skin collagen dramatically reduced HepG2 and HeLa cell growth by over 50% relative in a concentration-dependent manner when added to cells seeded 96-well plates. This suggests the collagen adding time was mightily important for effect of the collagen.

Cite this paper
nullHan, S. , Uzawa, Y. , Moriyama, T. and Kawamura, Y. (2011) Effect of collagen and collagen peptides from bluefin tuna abdominal skin on cancer cells. Health, 3, 129-134. doi: 10.4236/health.2011.33024.
References
[1]   Knekt, P., Jarvinen, R., Seppanen, R., Heliovaara, M., Teppo, L., Pukkala, E. and Aromaa, A. (1997) Dietary flavoniods and the risk of lung cancer other malignant neoplasmas. American Journal of Epidemology, 146, 223-230.

[2]   Jongjareonrak, A., Benjakul, S., Visessanguan, W., Nagai, T. and Tanaka, M. (2005) Isolation and charaterisation of acid and pepsin-solubilised collagens from skin of Brownstripe red snapper (Lutjanus vitta). Food Chemis-try, 93, 475-484. doi:10.1016/j.foodchem.2004.10.026

[3]   Mizuta, S., Fujisawa, S., Nishimoto, M. and Yoshinaka, R. (2005) Biochemical and immunochemical detection of type I and V collagens in tiger puffer Takifugu ruripes. Food Chemistry, 89, 373-377. doi:10.1016/j.foodchem.2004.02.043

[4]   Nagai, T. and Suzuki, N. (2002) Preparation and partial characterization of collagen from paper nautilus (Argo-nauta argo, Linnaeus) outer skin. Food Chemistry, 76, 149-153. doi:10.1016/S0308-8146(01)00255-2

[5]   Hwang, J.H., Mizuta, S., Yokoyama, Y. and Yoshinaka, R. (2005) Purification and characterization of molecular species of collagen in the skin of skate (Raja Kenojei). Food Chemistry, 100, 921-925. doi:10.1016/j.foodchem.2005.10.046

[6]   Tsuruoka, N., Yamato, R., Sakai, Y., Yoshitake, Y. and Yonekura, H. (2007) Promotion by collagen tripeptide of type I collagen gene expression in human osteoblastic cells and fracture healing of rat femur. Bioscience Bio-technology Biochemistry, 71, 2680-2687. doi:10.1271/bbb.70287

[7]   Gozalea, R.P., Leyva, A. and Moraes, O. (2001) Shark cartilage as source of antiangiogenic compound: from basic to clinic research. Biological & Pharmaceutical Bulletin, 24, 1097-1101. doi:10.1248/bpb.24.1097

[8]   Knekt, P., Jarvinen, R., Seppanen, R., Heliovaara, M., Teppo, L., Pukkala, E. and Aromaa, A. (1997) Dietary flavoniods and the risk of lung cancer other malignant neoplasmas. American Journal of Epidemology, 146, 223-230.

[9]   Omenn, G.S., Goodmay, G.E., Thornquist, M.D., Balmes, J., Cullen, M,R., Glass, A., Keogh, J.P., Meysken, F.L., Valanis, B., Williams, J., Barnhart, S. and Hammar, S. (1996) Effects of a combination of beta carotene and vi-tamin A on lung cancer and cardiovascular disease. The New England Journal of Medicine, 334, 1150-1155. doi:10.1056/NEJM199605023341802

[10]   Laemmli, U.K. (1970) Cleavage of structural proteins during assembly of head bacterriophage T4. Nature, 277, 680-685. doi:10.1038/227680a0

[11]   Zhang, Y., Liu W., Li, G., Shi, B., Miao, Y. and Wu, X., Physicochemical properties of collagen, gelatin and col-lagen hydroysate derived from bovine limed split waste. (2005) Food Chemistry, 103, 906-912. doi:10.1016/j.foodchem.2006.09.053

[12]   Achouri, A., Zhang, W. and Shiying, X. (1998) Isolation and partial characterization of pepsin-soluble collagen from the skin of grass carp (Ctenopharyngodon idella). Food Research International, 31, 617-623. doi:10.1016/S0963-9969(98)00104-5

[13]   Adler-Nissen, J. (1979) Enzymatic hydrolysis of soy protein isolate and effect of succinylation on the func-tional properties of resulting protein hydrolysates. Jour-nal of Agriculture Food Chemistry, 27, 1256-1262. doi:10.1021/jf60226a042

[14]   Shoji, Y., Isemura, M., Muto, H. and Isemura, S. (2000) Isolation of 41-kDa protein with cell adhesion activity for animal tumor cells from the mushroom Hypsizigus marmoreus by affinity chromatography with type IV col-lagen immobilized on agaros. Bioscience Biotechnology Biochemistry, 64, 775-780. doi:10.1271/bbb.64.775

[15]   Muyonga, J.H., Cole, C.G.B. and Doudu, K.G. (2004) Charaterisation of acid soluble collagen from skins of young and adult nile perch (Lates niloticus). Food Chemis-try, 85, 81-89. doi:10.1016/j.foodchem.2003.06.006

[16]   Yata, M., Yoshida, C., Fujisawa, S. and Yoshinaka, Y., (2001) Identification and characterization of molecular species of collagen in fish skin. Journal of Food Science, 66, 247-251. doi:10.1111/j.1365-2621.2001.tb11325.x

[17]   Nalinanon, S., Benjakul, S., Visessanguan, W. and Ki-shimura, H. (2007) Use of pepsine for collagen extrac-tion from the skin of bigeye smapper (Priaca nthus tay-enus). Food Chemistry, 104, 591-601. doi:10.1016/j.foodchem.2006.12.035

[18]   Muyonga, J.H., Cole, C.G.B. and Doudu, K.G. (2004) Charaterisation of acid soluble collagen from skins of young and adult nile perch (Lates niloticus). Food Chemistry, 85, 81-89. doi:10.1016/j.foodchem.2003.06.006

[19]   Nomura, Y., Sakai, H. and Shirai, K. (1996) Preparation and some properties of type I collagen from fish scales. Bioscience Biotechnology Biochemistry, 60, 2092-2094. doi:10.1271/bbb.60.2092

[20]   Nagai N., Mori K., Satoh Y., Takahashi N., Yunoki S., Tajima K. and Munekata M., (2007) In vitro growth and differentiated activities of human periodontal ligment fi-broblasts cultured salmon collagen gel. Journal of Bio-medical Materials Research Part A, 395-402. doi:10.1002/jbm.a.31110

[21]   Close, M.J., Howlett, A.R., Roskelley, C.D., Desprez, P.Y., Bailey, N., Rowning, B., Teng, C.T., Stampfer, M.R. and Yaswen, P. (1997) Lactoferrin expression in mam-mary epithelial cell is mediated by changes in cell shape and action cytoskeleton. Journal of Cell Science, 110, 2861-2871.

[22]   Hassan, Z.M., Feyzi, R., Sheikhian, A., Bargahi, A., Mo-stafaie, A., Mansouri, K., Shahrokhi, S., Ghazanfari, T. and Shahabi, S. (2005) Molecular weight fraction of shark cartilage can modulated immine responses and abolish angiogenesis. International Immunophamacology, 5, 961-970. doi:10.1016/j.intimp.2005.01.006

 
 
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