ENG  Vol.6 No.2 , February 2014
Molecular Characterization and mRNA Expression of Cytochrome P450 1A1 and Cytochrome P450 3A in Liver of Kafue Lechwe (Kobus leche kafuensis) as Potential Biomarkers of Pollution of the Kafue River Basin, Zambia
Abstract

The aim of this study was to characterize CYP1A1 and CYP3A in liver of the Kafue lechwe from Lochnivar and Blue Lagoon GMAs and to determine their mRNA expression levels as potential biomarkers of pollution. Increasing attention is paid to the problem of pesticide pollution by people. Risk assessment of pesticide should not be only based on the chemical analysis of environmental samples, since this method could not truly distinguish whether the pesticide was harmful to environment. Thus, biological assessment should be established.


Cite this paper
M’kandawire, E. , Syakalima, M. , Muzandu, K. , Pandey, G. , Simuunza, M. , Nakayama, S. , Kawai, Y. , Ikenaka, Y. and Ishizuka, M. (2014) Molecular Characterization and mRNA Expression of Cytochrome P450 1A1 and Cytochrome P450 3A in Liver of Kafue Lechwe (Kobus leche kafuensis) as Potential Biomarkers of Pollution of the Kafue River Basin, Zambia. Engineering, 6, 51-58. doi: 10.4236/eng.2014.62008.
References

[1]   W. A. Sheppe, “Effects of Human Activities on Zambia’s Kafue Flats Ecosystem,” Environmental Conservation, Vol. 12, No. 1, 1985, pp. 49-57.
http://dx.doi.org/10.1017/S0376892900015150

[2]   World Wildlife Fund, “Investing in the Kafue Flats Wetlands to Ensure Clean Water and Wildlife for Later Years,” 2001. http://www.partnersforwetlands.org/report/report-zambia

[3]   A. Gilman, “An Investigation into the Effects of Mining and Other Operations on Fish and Sediment Samples in the Chingola Area of the Zambian Copperbelt,” Environmental Research (Zambia), Mines Safety Department, Government of Zambia, Kitwe, 1997.

[4]   U. T. Pettersson and J. Ingri, “The Geochemistry of Co and Cu in the Kafue River as it Drains the Copperbelt Mining Area, Zambia,” Chemical Geology, Vol. 177, No. 3-4, 2000, pp. 399-414.
http://dx.doi.org/10.1016/S0009-2541(00)00422-8

[5]   U. T. Pettersson, J. Ingri and P. S. Andersson, “Hydrogeochemical Processes in the Kafue River Upstream from the Copperbelt Mining Area, Zambia,” Aquatic Geochemistry, Vol. 6, No. 4, 2000, pp. 385-411.
http://dx.doi.org/10.1023/A:1009610327246

[6]   L. Norrgren, U. Pettersson, S. Orn and P. A. Bergqvist, “Environmental Monitoring of the Kafue River, Located in the Copperbelt, Zambia,” Archives of Environmental Contamination and Toxicology, Vol. 38, No. 3, 2000, pp. 334-341. http://dx.doi.org/10.1007/s002449910044

[7]   M. Syakalima, K. Choongo, R. Mwenechanya, V. Wepener, M. Yamasaki and Y. Maede, “Pesticide/Herbicide Pollutants in the Kafue River and a Preliminary Investigation into Their Biological Effect through Catalase Levels in Fish,” Japan Journal of Veterinary Research, Vol. 54, 2006, pp. 119-128.

[8]   K. Sichilongo and N. Torto, “Determination of Endocrine Disruptors in Kafue Lechwe (Kobus Leche Kafuensis) Samples from the Lochinvar National Park of Zambia,” Environment International, Vol. 32, No. 6, 2006, pp. 718723. http://dx.doi.org/10.1016/j.envint.2006.03.007

[9]   T. D. Porter and M. J. Coon, “Cytochrome P450, Multiplicity of Isoforms, Substrates, and Catalytic and Regulatory Mechanisms,” Journal of Biological Chemistry, Vol. 266, 1991, pp. 13469-13472.

[10]   D. R. Nelson, L. Koymans, T. Kamataki, J. J. Stegeman, R. Feyereisen, D. J. Waxman, M. R. Waterman, O. Gotoh, M. J. Coon, R. W. Estabrook, I. C. Gunsalus and D. W. Nebert, “P-450 Superfamily: Update on New Sequence, Gene Mapping, Accession Numbers and Nomenclature,” Pharmacogenetics, Vol. 6, 1991, pp. 1-42.
http://dx.doi.org/10.1097/00008571-199602000-00002

[11]   J. J. Stegeman and D. R. Livingstone, “Forms and Functions of Cytochrome P450,” Comparative Biochemistry and Physiology C Toxicology Pharmacology, Vol. 121, 1998, pp. 1-3.

[12]   J. J. Stegeman and M. E. Hahn,” Biochemistry and Molecular Biology of Monooxygenases: Current Perspectives on Forms, Functions, and Regulation of Cytochrome P450 in Aquatic Species,” In: D. C. Malins and G. K. Ostrander, Eds., Aquatic Toxicology, Molecular, Biochemical and Cellular Perspectives, Lewis Publishers, Boca Raton, 1994, pp. 87-206.

[13]   A. Goksoyr, “Cytochrome P450 in Marine Mammals: Isoenzyme Forms, Catalytic Functions, and Physiological Regulations,” In: A. S. Blix, L. Walloe and O. Ulltang, Eds., Whales, Seals, Fish and Man, Elsevier Science, Amsterdam, 1995, pp. 629-639.

[14]   P. Maurel, “The CYP 3 Family,” In: C. Loannides, Ed., Cytochromes P450 Metabolic and Toxicological Aspects, CRC Press, Boca Raton, 1996, pp. 241-720.

[15]   R. W. Nims and R. A. Lubet, “Induction of Cytochrome P-450 in the Norway Rat, (Rattus Norvegicus), Following Exposure to Potential Environmental Contaminants,” Journal of Toxicology and Environmental Health, Vol. 46, No. 3, 1995, pp. 271-292.
http://dx.doi.org/10.1080/15287399509532035

[16]   J. F. Gracey and D. S. Collins, “Meat Hygiene,” Ballière Tindall, London, 1992.

[17]   P. Chomczynski and N. Sacchi, “Single-Step Method of Isolation by Acid Guanidinium Thiocyanate-Phenol-Chloroform Extraction,” Analytical Biochemistry, Vol. 162, No. 1, 1987, pp. 156-159.
http://dx.doi.org/10.1016/0003-2697(87)90021-2

[18]   K. A. Youdim, C. A. Tyman, B. C. Jones and R. Hyland, “Induction of Cytochrome P450: Assessment in an Immortalized Human Hepatocyte Cell Line (Fa2N4) Using a Novel Higher throughput Cocktail Assay,” Drug Metabolism and Disposition, Vol. 35, No. 2, 2007, pp. 275282. http://dx.doi.org/10.1124/dmd.106.012864

[19]   E. M’kandawire, M. Syakalima, K. Muzandu, G. Pandey, M. Simuunza, S. M. M. Nakayama, Y. K. Kawai, Y. Ikenaka and M. Ishizuka, “The Nucleotide Sequence of Metallothioneins (MT) in Liver of the Kafue Lechwe (Kobus Leche Kafuensis) and Their Potential as Biomarkers of Heavy Metal Pollution of the Kafue River,” Gene, Vol. 506, No. 2, 2012, pp. 310-316.
http://dx.doi.org/10.1016/j.gene.2012.07.002

[20]   J. D. Thompson, D. G. Higgins and T. J. Gibson, “CLUSTAL W: Improving the Sensitivity of Progressive Multiple Sequence Alignment through Sequence Weighting, Positions-Specific Gap Penalties and Weight Matrix Choice,” Nucleic Acids Research, Vol. 22, No. 22, 1994, pp. 46734680. http://dx.doi.org/10.1093/nar/22.22.4673

[21]   K. Kawajiri, O. Gotoh, K. Sogawa, Y. Tagashira, M. Muramatsu and Y. Fujii-Kuriyama, “Coding Nucleotide Sequence of 3-Methylcholanthrene-Inducible Cytochrome P450d cDNA from Rat Liver,” Proceedings of the National Academy of Sciences of the USA, 1984, pp. 16491653.

[22]   D. W. Nebert and F. J. Gonzalez, “P450 Genes: Structure, Evolution, and Regulation,” Annual Review of Biochemistry, Vol. 56, 1987, pp. 945-993.
http://dx.doi.org/10.1146/annurev.bi.56.070187.004501

[23]   F. J. Gonzalez, “The Molecular Biology of Cytochrome P450s,” Pharmacology Reviews, Vol. 40, 1988, pp. 243288.

[24]   F. J. Gonzalez, D. W. Nebert, J. P. Hardwick and C. B. Kasper, “Complete cDNA and Protein Sequence of a Pregnenolone 16-a-Carbonitrile-Induced Cytochrome P-450. A Representative of a New Gene Family,” Journal of Biological Chemistry, Vol. 260, 1985, pp. 7435-7441.

[25]   M. A. Kimura, “Simple Method for Estimating Evolutionary Rate of Base Substitutions through Comparative Studies of Nucleotide Sequences,” Journal of Molecular Evolution, Vol. 16, No. 2, 1980, pp. 111-120.
http://dx.doi.org/10.1007/BF01731581

[26]   Z. Yang, “PAML: A Program Package for Phylogenetic Analysis by Maximum Likelihood,” Computer Applications in the Biosciences, Vol. 13, 1997, pp. 555-556.

[27]   M. Pfister-Genskow, C. Myers, L. A. Childs, J. C. Lacson, T. Patterson, J. M. Betthauser, P. J. Goueleke, R. W. Koppang, G. Lange and P. Fisher, “Identification of Differentially Expressed Genes in Individual Bovine Preimplantation Embryos Produced by Nuclear Transfer: Improper Reprogramming of Genes Required for Development,” Biology of Reproduction, Vol. 72, No. 3, 2005, pp. 546-555. http://dx.doi.org/10.1095/biolreprod.104.031799

[28]   K. Tamura, D. Peterson, N. Peterson, G. Stecher, M. Nei and S. Kumar, “MEGA5: Molecular Evolutionary Genetics Analysis Using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods,” Molecular Biology and Evolution, Vol. 28, No. 10, 2011, pp. 27312739. http://dx.doi.org/10.1093/molbev/msr121

[29]   D. W. Anderson and J. J. Hickey, “Eggshell Changes in Certain North American Birds,” In: K. H. Voous, Ed., Proceedings of the 15th International Ornithological Congress, Leiden, 1972, pp. 514-540.

[30]   D. W. Nebert and D. W. Russell, “Clinical Importance of the Cytochromes P450,” Lancet, Vol. 360, No. 9340, 2002, pp. 1155-1162.
http://dx.doi.org/10.1016/S0140-6736(02)11203-7

[31]   D. Rottcher, “Veterinary Wildlife Research 1975-1998,” Final Report to the Zambian Government, 1998.

 
 
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