WJV  Vol.4 No.1 , February 2014
Field Trial of a Thermostable Peste des petits ruminants (PPR) Vaccine in a Semi-Arid Zone of Nigeria
Abstract: The field trial of a candidate thermostable Peste des petits ruminants (PPR) vaccine was carried out in flocks of sheep and goats under the extensive system of management. The immune response of vaccinated animals was determined using the neutralisation test to detect PPR virus specific antibody. Vaccinated animals seroconverted and a four-fold or more rise in antibody titre were observed between pre-vaccination and post-vaccination antibodies. The vaccine elicited significant antibody response in goats through the different routes of administration (intramuscular, intranasal, intraocular, subcutaneous and orally), but was poorly transmitted between the vaccinees and in-contact animals. The sheep responded poorly to the vaccine administered through most of the routes, except for those vaccinated through intramuscular and subcutaneous routes that seroconverted significantly (≥4 fold rise). The vaccine retained a potent titre of 3.1 log10 TCID50 for more than 8 hours after reconstitution in PBS at room temperature. Based on the response of goats to oral vaccination, it is suggested that the vaccine could be administered on the field through the oral routes and has the potential to be adapted to a feed-based administration for wider application to the scattered livestock populations under the extensive system of management.
Cite this paper: A. El-Yuguda, S. Baba, A. Ambali and G. Egwu, "Field Trial of a Thermostable Peste des petits ruminants (PPR) Vaccine in a Semi-Arid Zone of Nigeria," World Journal of Vaccines, Vol. 4 No. 1, 2014, pp. 1-6. doi: 10.4236/wjv.2014.41001.

[1]   M. P. Siddique, M. B. Rahman, S. M. Z. H. Chowdhury, M. A. Kafi and M. S. Alam, “Determination of Efficacy of Thermostable ppr Live Homologous Vaccine Incubated at Room Temperature for 14 Days,” Bangladash Journal of Veterinary Medicine, Vol. 4, No.1, 2006, pp. 43-46.

[2]   M. Munir, S. Zohari and M. Berg, “Pathophysiology and Clinical Assessment of Peste des Petits Ruminants,” In: Munir et al., Ed., Molecular Biology and Pathogenesis of Peste des Petits Ruminants Virus. A Springer Briefs in Animal Sciences Pub., New York, 2013, pp. 33-47.

[3]   A. D. El-Yuguda, L. M. Chabiri, F. Adamu and S. S. Baba, “Peste des petits ruminants Virus (PPRV) Infection among Small Ruminants Slaughtered at the Central Abattoir, Maiduguri, Nigeria,” Sahel Journal of Veterinary Science, Vol. 8, No. 2, 2009, pp. 93-96.

[4]   A. Sen, P. Saravanan, V. Balamurugan, K. K. Rajak, S. B. Sudhakar, V. Bhanuprakash, S. Parida and R. K. Singh, “Vaccines against Peste des petits ruminants Virus,” Expert Review of Vaccines, Vol. 9, No. 7, 2010, pp. 785-796.

[5]   A. D. El-Yuguda, D. N. Bukbuk and A. Andrew, “Response of Sahel Goats tp Homologous PPR Vaccine (CapripestovaxR) under Field Conditions in Maiduguri, Nigeria,” Tropical Veterinarian, Vol. 29, No. 4, 2011, pp. 21-26.

[6]   F. D. Adu and T. E. Joannis, “Serum-Virus Simultaneous Method of Immunization against PPR,” Tropical Animal Health and Production, Vol. 16, No. 2, 1984, pp. 119-122.

[7]   L. Gargadenne and A. Lalanne, “La Peste des Petits Ruminanats” Bulletin des Services Zootechniques et des Epizooties de l’Afrique Occidentale Fran?aise, Vol. 5, No. 1, 1942, pp. 16-21.

[8]   P. Mornet, J. Orue, Y. Gilbert, G. Thiery and M. Sow, “La peste des petits ruminants en Afrique Occidentale Francaise: Ses Rapports Avec la Peste Bovine,” Revue d’élevage et de Médecine Vétérinaire des Pays Tropicaux, Vol. 9, 1956, pp. 313-335.

[9]   C. A. Mebus and M. C. Van den Ende, “The Use of Thermostable Vero Cell-Adapted Rinderpest Vaccine as a Heterologous Vaccine against Peste des petits ruminants,” Researches in Veterinary Science, Vol. 54, No. 2, 1990, pp. 212-216.

[10]   A. Diallo, T. Barrett, M. Barbron, M. S. Shaila and W. P. Taylor, “Differentiation of Rinderpest and Peste des petits ruminants Viruses Using Specific cDNA Clones,” Journal of Virological Methods, Vol. 23, No. 2, 1989, pp. 127-136.

[11]   W. Chen, S. Hu, L. Qu, Q. Hu, Q. Zhang, H. Zhi, K. Huang and Z. Bu, “A Goat Poxvirus-Vectored Peste-Des-Petits-Ruminants Vaccine Induces Long-Lasting Neutralization Antibody to High Levels in Goats and Sheep,” Vaccine, Vol. 28, No. 30, 2010, pp. 4742-4750.

[12]   J. Qin, H. Huang, Y. Ruan, X. Hou, S. Yang, C. Wang, G. Huang, T. Wang, N. Feng, Y. Gao and X. Xia, “A Novel Recombinant Peste des petits ruminants-Canine Adenovirus Vaccine Elicits Long-Lasting Neutralizing Antibody Response against PPR in Goats,” PLoS One, Vol. 7, No. 5, 2012, p. E37170.

[13]   D. N. Awa, A. Ngagnou, E. Tefiang, D. Yaya and A. Njoya, “Post Vaccination Colostral Peste des petits ruminants Antibody Dynamics in Research Flocks of Kirdi Goats and Foulbe Sheep of North Cameron,” Preventive Veterinary Medicine, Vol. 5, 2002, pp. 267-271.

[14]   S. S. Baba, A. D. El-Yuguda, G. O. Egwu, A. Y. Ribadu, A. G. Ambali, M. D. Abubakar, U. I. Ibrahim and N. Zoyem, “Development and Evaluation of the Efficacy of Heat Tolerant Peste des petits ruminants (PPR) Vaccine in Nigeria,” Proceedings of International Conference on Adaptive Science & Technology (ICAST), Accra, Ghana, 2007, pp. 129-132

[15]   E. E., Worrall, J. K. Litamoi, B. M. Seck and G. Ayelet, “Xerovac: An Ultra Rapid Method for the Dehydration and Preservation of Live Attenuated Rinderpest and Peste des petits ruminants Vaccines,” Vaccine, Vol. 19, No. 7-8, 2001, pp. 834-839.

[16]   A. D. El-Yuguda, S. S. Baba, A. G. Ambali and G. O. Egwu, “Seroprevalence of Peste des petits ruminants among Domestic Small and Large Ruminants in the Semi-Arid Region of North-Eastern Nigeria,” Veterinary World, Vol. 6, No. 10, 2013, pp. 807-811.

[17]   CDC (Centre for disease control), “Descriptive Statistics: Measure of Central Tendency and Dispersion,” Centre for Disease Control and Prevention Atlanta Georgia USA, 1988.

[18]   S. C. Das, M. D. Baron, M. A. Skinner and T. Barrett, “Improved Technique for Transient Expression and Negative Strand Virus Rescue Using Fowl Pox T7 Recombinant Virus in Mammalian Cells,” Journal of Virological Methods, Vol. 89, No. 1-2, 2000, pp. 119-127.

[19]   J. C. Mariner, J. A. House, C. A. Mebus and M. C. Van den Ende, “The Use of Thermostable Vero Cell-Adapted Rinderpest Vaccine as a Heterologous Vaccine against Peste des petits ruminants,” Research in Veterinary Science, Vol. 54, No. 2, 1990, pp. 212-216.

[20]   E. Couacy-hymann, S. C. Bodjo, T. Danho, M. Y. Koffi, G. Libeau and A. Diallo, “Early Detection of Viral Excretion from Experimentally Infected Goats with Pets des Petits Ruminants Virus,” Preventive Veterinary Medicine, Vol. 78, No. 1, 2007, pp. 85-88.

[21]   J. Sarkar, B. P. Sreenivasa, R. P. Singh, P. Dhar and S. K. Bandyopadhyay, “Comparative Efficacy of Various Chemical Stabilizers on the Thermostability of a Live Attenuated Peste des petits ruminants (PPR) Vaccine,” Vaccine, Vol. 21, No. 32, 2003, pp. 4728-4735.