Tetrazolium Test to Evaluate Stizolobium aterrimum Seeds Quality

Bruno B.  Deminicis; Patricia Do R.  Rodrigues; Bráulio P.  Faria; Henrique D.  Vieira; Antônio D.  Pandolfi Filho; Guilherme S.  Freitas

doi:10.4236/ajps.2014.51019

Bruno B. Deminicis^*, Patricia Do R. Rodrigues, Bráulio P. Faria, Henrique D. Vieira, Antônio D. Pandolfi Filho, Guilherme S. Freitas

Abstract: The purpose of this study was to verify the efficiency of methods of pre-conditioning and concentrations of tetrazolium solution in the evaluation of physiological quality of Stizolobium aterrimum seeds. Commercial seeds have passed through the process of accelerated aging for composing lots of 0, 24, 48, 96 and 144 hours, and were submitted to the pre-conditioning: 1) Scarification with sandpaper number 100 and immersion in water at room temperature for 18 hours, 2) Immersion in water at 95°C and maintained in the same water outside the heating at 25°C for 18 hours. After preconditioning, the seed coats were removed and immersed in embryos tetrazolium salt at 0.25%, 0.5% and 1% for 150 minutes and maintained in the dark at 25°C for color development. To compare the results obtained in the tetrazolium test, we performed germination test, first counting and germination speed index. The method of scarification with sandpaper and later soaked in water for 18 hours at 25°C, the most efficient way in preconditioning of seeds of Stizolobium aterrimum and concentration of 1.0% tetrazolium solution for 150 minutes at 25°C, allows evaluating the quality of seed lots from this species.

Keywords: Germination; Forage Legumes; Tetrazolium Test

Cite this paper: B. Deminicis, P. Rodrigues, B. Faria, H. Vieira, A. Pandolfi Filho and G. Freitas, "Tetrazolium Test to Evaluate Stizolobium aterrimum Seeds Quality," American Journal of Plant Sciences, Vol. 5 No. 1, 2014, pp. 148-152. doi: 10.4236/ajps.2014.51019.

References

[1] IBGE Brazilian Institute of Geography and Statistics, “IBGE Reveals the Milk Production Growth,” 2010.
http://www.portaldoagronegocio.com.br/conteudo.php?id=34328

[2] M. C. Bhering, D. C. F. S. Dias and D. I. Barros, “Methodology Appropriatenes of the Tetrazolium Test to Evaluate the Physiological Quality of Watermelon Seeds,” Brazilian Journal of Seeds, Vol. 27, No. 1, 2005, pp. 176-182. www.scielo.br/pdf/rbs/v27n1/25196.pdf

[3] J. Marcos Filho, S. M. Cícero and W. R. Silva, “Seeds Quality Evaluation,” FEALQ, Piracicaba, 1987, 230 p.

[4] J. B. França Neto, F. C. Krzyzanowski and N. P. Costa, “Tetrazolium Test in Soy Seeds,” EMBRAPA, Londrina, 1998, 72 p.
http://www.cnpso.embrapa.br/download/pdf/cirtec39_sementes.pdf

[5] L. M. Oliveira, M. L. M. Carvalho and A. C. Davide, “Tetrazolium Test to Evaluate the Quality of Peltophorum dubium (Sprengel) Taubert—Leguminosea Caesalpinioidae Seeds,” Ufla Journal, Vol. 11, No. 2, 2005, pp. 159-166. http://www.dcf.ufla.br/cerne/artigos/11-02-20097975v11_n2_artigo%2006.pdf

[6] Brasil Ministry of Agriculture, “Rules for Seeds Analysis,” CLAV/DNDV/SNAD, Brasília, 2009, 365 p.
www.bs.cca.ufsc.br/publicacoes/regras%20analise%20sementes.pdf

[7] J. D. Maguire, “Speed of Germination Aid in Selection and Evaluation for Seeding Emergences and Vigor,” Crop Science, Vol. 2, No. 2, 1962, pp. 196-197.

[8] D. F. Ferreira, “System for Analysis of Variance for Balance Data,” SISVAR Vesion 5.1, UFLA, Lavras, 2007.
www.dex.ufla.br/~danielff/softwares.htm

[9] C. M. G. Oliveira, C. C. Martins and J. Nakagawa, “Preparation Methodology for the Tetrazolium Test in Ricinuscommunis L. Seeds,” Brazilian Journal of Seeds, Vol. 31, No. 1, 2009, pp. 160-167.
http://www.scielo.br/pdf/rbs/v31n1/a06v31n1.pdf

[10] C. A. Fogaça, C. Zucareli, M. M. Malavasi, C. Zucareli and U. C. Malavasi, “Use of Tetrazolium Test in Gleditschia amorphoides Taub. Caesalpinaceae Seeds,” Brazilian Journal of Seeds, Vol. 28, No. 3, 2006, pp. 101-107.
http://www.scielo.br/pdf/rbs/v28n3/15.pdf

[11] R. A. Ferreira, L. M. Oliveira, O. A. O. Tonetti and A. C. Davide, “Viability Comparison of the Schizolobium parahyba (Vell) Blake—Leguminosae Caesalpinaceae Seeds by the Germination and Tetrazolium Tests,” Brazilian Journal of Seeds, Vol. 29, No. 3, 2007, pp. 83-89.
http://www.scielo.br/pdf/rbs/v29n3/a11v29n3.pdf

[12] E. A. F. Mendonça, M. F. B. Coelho and M. Luchese, “Tetrazolium Test Mangaba-Brava (Lafoensiapacari St. Hil.-Lythraceae) Seeds,” Brazilian Journal of Medicinal Plants, Vol. 8, No. 2, 2006, pp. 33-38.
www.ibb.unesp.br/servicos/publicacoes/...v8_n2_2006/artigo6_v8_n2.pdf

[13] E. A. F. Mendonça, N. P. Ramos and R. C. Paula, “Cordiatrichotoma (Vellozzo) Arrabida ex Steudel (Louro-Pardo) Seeds Viability by Tetrazolium Test,” Brazilian Journal of Seeds, Vol. 23, No. 2, 2001, pp. 64-71.
www.abrates.org.br/revista/artigos/2001/v23n2/artigo09.pdf

[14] C. J. Costa and P. C. Santos, “Tetrazolium Test in Leucena Seeds,” Brazilian Journal of Seeds, Vol. 32, No. 2, 2010, pp. 66-72.
www.scielo.br/pdf/rbs/v32n2/v32n2a08.pdf

[15] D. I. Barros, D. C. F. S. Dias, M. C. Bhering, L. A. S. Dias and E. F. Araújo, “Use of Tetrazolium Test to Evaluate the Physiological Quality of the Zucchini Seeds,” Brazilian Journal of Seeds, Vol. 27, No. 2, 2005, pp. 165-171. www.scielo.br/pdf/rbs/v27n2/a24v27n2.pdf

[16] R. C. Souza, C. O. Ohlson and M. Panobianco, “Viability Evaluation of Black Whey Seeds by the Tetrazolium Test,” Brazilian Journal of Seeds, Vol. 31, No. 3, 2009, pp. 57-62. http://www.scielo.br/pdf/rbs/v31n3/a06v31n3.pd

[17] C. Zucareli, M. M. Malavasi, C. A. Fogaça and V. C. Malavasi, “Preparation and Coloring of Albiziahasslerii (Chodat) Bur.) Seeds for the Tetrazolium Test,” Brazilian Journal of Seeds, Vol. 23, No. 2, 2001, pp. 186-191.
http://www.abrates.org.br/revista/artigos/2001/v23n2/artigo26.pdf

[18] S. da M. A. Mendes, A. A. de Bastos and G. G. da. M. de. Melo, “Standardization of Tetrazolium Test for Parkiavelutina Benoist (Leguminosae-Mimosoideae) Seeds,” Acta Amazonica, Vol. 39, No. 4, 2009, pp. 823-828.
http://www.scielo.br/pdf/aa/v39n4/v39n4a10.pdf
http://dx.doi.org/10.1590/S0044-59672009000400010

[19] L. M. M. Añez, M. F. B. Coelho, M. C. F. E. Albuquerque, J. L. D. Dombroski and E. A. F. Mendonca, “Methodology Standardization of Tetrazolium Test for Jatrophaelliptica M. Arg. (Euphorbiaceae) Seeds,” Brazilian Journal of Medicinal Plants, Vol. 9, 2007, pp. 82-88.
http://www.ibb.unesp.br/servicos/publicacoes/rbpm/pdf_v9_n3_2007/artigo12_v9_n3.pdf

[20] B. B. Deminicis, H. D. Vieira and R. F. Da Silva, “Tetrazolium Test to Evaluate the Quality of Clitoreaternatea Seeds L,” Brazilian Journal of Seeds, Vol. 31, No. 1, 2009, pp. 12-19.
http://www.scielo.br/pdf/rbs/v31n2/v31n2a06.pdf

[21] T. L. F. Pinto, P. H. S. Brancalion, A. D. L. C. Novembre and S. M. Cicero, “Viability Evaluation of Poecilantheparviflora Benth.—Fabaceae—Faboideae Seeds by the Tetrazolium Test,” Brazilian Journal of Seeds, Vol. 30, No. 1, 2008, pp. 208-214.
http://www.scielo.br/pdf/rbs/v30n1/a26v30n1.pdf.