In-Situ Synthesized Myoglobin Imprinted Poly(N-Isopropylacrylamide)/Silica Nanocomposite Gels by Using the Molecular Imprinting Method

Burcu  Meşhur; Eylem  Turan; Nursel  Dilsiz

doi:10.4236/ampc.2014.41001

Burcu Meşhur, Eylem Turan, Nursel Dilsiz^*

Abstract: Temperature sensitive imprinted poly(N-isopropylacrylamide) nanocomposite gels were syntheses via in-situ, free radical crosslinking polymerization of corresponding monomer in nano-sized silica and five different concentrations of myoglobin solution by using the molecular imprinting method. Mb adsorption from five different concentrations of Mb solutions was investigated by two types of nanocomposite gel systems prepared by non-imprinted and imprinted methods. Nanocomposite gels imprinted with Mb showed higher adsorption capacity and specificity for Mb than nanocomposite gels prepared by the usual procedure. The highest Mb adsorption was observed via the imprinted nanocomposite gels with 12.5% Mb. In addition, selectivity studies were also performed by using two reference molecules as fibrinogen and hemoglobin. The imprinted nanocomposite gels had higher adsorption capacity for Mb than the non-imprinted gels and also exhibited good selectivity for Mb and high adsorption rate depending on the number of Mb sized cavities.

Keywords: Nanocomposite Gels; Poly(N-Isopropylacrylamide); Temperature Sensitive; Myglobin; Free Radical Crosslinking Polymerization; Adsorption; Imprinted

Cite this paper: B. Meşhur, E. Turan and N. Dilsiz, "In-Situ Synthesized Myoglobin Imprinted Poly(N-Isopropylacrylamide)/Silica Nanocomposite Gels by Using the Molecular Imprinting Method," Advances in Materials Physics and Chemistry, Vol. 4 No. 1, 2014, pp. 1-5. doi: 10.4236/ampc.2014.41001.

References

[1] C. Alexander, H. S. Andersson, L. I. Andersson, R. J. Ansell, N. Kirsch, I. A. Nicholls, J. O’Mahony and M. J. Whitcombe, “Molecular Imprinting Science and Technology: A Survey of the Literature for the Years up to and Including 2003,” Journal of Molecular Recognition, Vol. 19, No. 2, 2006, pp. 106-180.
http://dx.doi.org/10.1002/jmr.760

[2] J. Q. Liu and G. Wulff, “Molecularly Imprinted Polymers with Strong Carboxypeptidase A-Like Activity: Combination of an Amidinium Function with a Zinc-Ion Binding Site in Transition-State Imprinted Cavities,” Angewandte Chemie International Edition, Vol. 43, No. 10, 2004, pp. 1287-1290.
http://dx.doi.org/10.1002/anie.200352770

[3] D. Carboni, K. Flavin, A. Servant, V. Gouverneur and M. Resmini, “The First Example of Molecularly Imprinted Nanogels with Aldolase Type I Activity,” Chemistry—A European Journal, Vol. 14, No. 23, 2008, pp. 7059-7065.

[4] S. Shiho, H. Shiigi and T. Nagaoka, “Review: Microand Nanosized Molecularly Imprinted Polymers for HighThroughput Analytical Applications,” Analytica Chimica Acta, Vol. 641, No. 1-2, 2009, pp. 7-13.
http://dx.doi.org/10.1016/j.aca.2009.03.035

[5] A. Poma, A. P. F. Turner and S. A. Piletsky, “Advances in the Manufacture of MIP Nanoparticles,” Trends in Biotechnology, Vol. 28, No. 12, 2010, pp. 629-637.
http://dx.doi.org/10.1016/j.tibtech.2010.08.006

[6] K. Rostamizadeh, M. Vahedpour and S. Bozorgi, “Synthesis, Characterization and Evaluation of Computationally Designed Nanoparticles of Molecular Imprinted Polymers as Drug Delivery Systems,” International Journal of Pharmaceutics, Vol. 424, No. 1-2, 2012, pp. 67-75.
http://dx.doi.org/10.1016/j.ijpharm.2011.12.054

[7] Y. Q. Lv , T. W. Tan and F. Svec, “Molecular Imprinting of Proteins in Polymers Attached to the Surface of Nanomaterials for Selective Recognition of Biomacromolecules,” Biotechnology Advances, Vol. 31, No. 8, 2013, pp. 1172-1186.

[8] H. Chen, D. Yuan, Y. Li, M. Dong, Z. Chai, J. Kong and G. Fu, “Silica Nanoparticle Supported Molecularly Imprinted Polymer Layers with Varied Degrees of Crosslinking for Lysozyme Recognition,” Analytica Chimica Acta, Vol. 779, 2013, pp. 82-89.
http://dx.doi.org/10.1016/j.aca.2013.03.052

[9] G. Pan, B. Zu, X. Guo, Y. Zhang, C. Li and H. Zhang, “Preparation of Molecularly Imprinted Polymer Microspheres via Reversible Addition-Fragmentation Chain Transfer Precipitation Polymerization,” Polymer, Vol. 50, No. 13, 2009, pp. 2819-2825.
http://dx.doi.org/10.1016/j.polymer.2009.04.053

[10] A. Uysal, G. Demirel, E. Turan and T. Caykara, “Dependence of Fibrinogen Recognition of Molecularly Imprinted Hydrogels on Preparation PH,” Analytica Chimica Acta, Vol. 625, No. 1, 2008, pp. 110-115.
http://dx.doi.org/10.1016/j.aca.2008.07.010

[11] D. Farnan, D. D. Frey and C. Horvath, “Intraparticle Mass Transfer in High-Speed Chromatography of Proteins,” Biotechnology Progress, Vol. 13, No. 4, 1997, pp. 429-439. http://dx.doi.org/10.1021/bp970044k