We introduce a homology-based
recombination approach for generating a cDNA construct. This method depends on amplifying
several exon fragments and their fusions by the homology-based recombination. This
method provides a way to generate the cDNA sequence of any gene without any
need for its mRNA. The paper describes the
strategy by assembling cDNA of the MYB1
and MYB2 genes of Arabidopsis thaliana.
Cite this paper
Verma, N. and Burma, P. (2014) A method to synthesize cDNA constructs by homology based recombination cloning. American Journal of Molecular Biology
, 16-19. doi: 10.4236/ajmb.2014.41003
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