A method to synthesize cDNA constructs by homology based recombination cloning
ABSTRACT
We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.
Cite this paper
Verma, N. and Burma, P. (2014) A method to synthesize cDNA constructs by homology based recombination cloning. American Journal of Molecular Biology, 4, 16-19. doi: 10.4236/ajmb.2014.41003.
Verma, N. and Burma, P. (2014) A method to synthesize cDNA constructs by homology based recombination cloning. American Journal of Molecular Biology, 4, 16-19. doi: 10.4236/ajmb.2014.41003.
References
[1] Sleight, S.C., Bartley, B.A., Lieviant, J.A. and Sauro, H.M. (2010) In-fusion biobrick assembly and re-engineering. Nucleic Acids Research, 38, 2624-2636.
http://dx.doi.org/10.1093/nar/gkq179 [2] Topfer, R., Matzeit, V., Bruno, G., Schell, J. and Steinbiss, H.H. (1987) A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Research, 15, 5890.
[1] Sleight, S.C., Bartley, B.A., Lieviant, J.A. and Sauro, H.M. (2010) In-fusion biobrick assembly and re-engineering. Nucleic Acids Research, 38, 2624-2636.
http://dx.doi.org/10.1093/nar/gkq179 [2] Topfer, R., Matzeit, V., Bruno, G., Schell, J. and Steinbiss, H.H. (1987) A set of plant expression vectors for transcriptional and translational fusions. Nucleic Acids Research, 15, 5890.