We introduce a homology-based recombination approach for generating a cDNA construct. This method depends on amplifying several exon fragments and their fusions by the homology-based recombination. This method provides a way to generate the cDNA sequence of any gene without any need for its mRNA. The paper describes the strategy by assembling cDNA of the MYB1 and MYB2 genes of Arabidopsis thaliana.
 Sleight, S.C., Bartley, B.A., Lieviant, J.A. and Sauro, H.M. (2010) In-fusion biobrick assembly and re-engineering. Nucleic Acids Research, 38, 2624-2636.