JASMI  Vol.3 No.4 , December 2013
Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer
Author(s) Alfons Penzkofer*
ABSTRACT

In the sample compartment of a conventional spectrophotometer, mounting of a polarizer before sample and an analyzer behind sample allows the determination of the optical rotatory dispersion of optical active media by measurement of the transmission ratio of crossed and parallel arranged polarizer and analyzer. A formula for the determination of the angle of rotation is derived from the transmission ratio. The arrangement is applied to determine the molar optical rotation of D-glucose in water in the wavelength range from 220 nm to 820 nm.


Cite this paper
A. Penzkofer, "Optical Rotatory Dispersion Measurement of D-Glucose with Fixed Polarizer Analyzer Accessory in Conventional Spectrophotometer," Journal of Analytical Sciences, Methods and Instrumentation, Vol. 3 No. 4, 2013, pp. 234-239. doi: 10.4236/jasmi.2013.34030.
References
[1]   L. D. Barron, “Molecular Light Scattering and Optical Activity,” 2nd Edition, Cambridge University Press, Cambridge, 2009.

[2]   E. Charney, “The Molecular Basis of Optical Activity. Optical Rotatory Dispersion and Circular Dichroism,” John Wiley & Sons, New York, 1979.

[3]   P. Grabbé, “Optical Rotatory Dispersion and Circular Dichroism in Organic Chemistry,” Holden-Day, San Francisco, 1965.

[4]   A. Moscowitz, “Theoretical Aspects of Optical Activity. Part One: Small Molecules,” In: I. Prigogine, Ed., Advances in Chemical Physics, Vol. IV, Interscience Publishers, New York, 1972, pp. 67-112.

[5]   S. F. Mason, “Molecular Optical Activity and the Chiral Discriminations,” Cambridge University Press, Cambridge, 1982.

[6]   E. M. Slayter, “Optical Methods in Biology,” Wiley-Interscience, New York, 1970, Chapter 27.

[7]   D. E. McCarthy, “Transmittance of Optical Materials from 0.17 μ to 3.0 μ,” Applied Optics, Vol. 6, No. 8, 1967, pp. 1896-1898.

[8]   F. Wu, G. Li, J. Huang and D. Yu, “Calcite/Barium Fluoride Ultraviolet Polarizing Prism,” Applied Optics, Vol. 34, No. 19, 1995, pp. 3668-3670.
http://dx.doi.org/10.1364/AO.34.003668

[9]   V. P. Solntsev, E. G. Tsvetkov, V. A. Gets and V. D. Antsygin, “Growth of α-BaB2O4 Single Crystals from Melts at Various Compositions: Comparison of Optical properties,” Journal of Crystal Growth, Vol. 236, 2002, pp. 290-296. http://dx.doi.org/10.1016/S0022-0248(01)02216-3

[10]   I. Listowsky, G. Avigad and S. Englard, “Optical Rotatory Dispersion of Sugars. I. Relationship to Configuration and Conformation of Aldopyranoses,” Journal of the American Chemical. Society, Vol. 87, No. 8, 1965, pp. 1765-1771. http://dx.doi.org/10.1021/ja01086a027

[11]   S. Budavari, “The Merck Index. An Encyclopedia of Chemicals, Drugs, and Biologicals,” 13th Edition, Entry# 4353 Glucose, Merck & Co., Inc., Rahway, 1989, p. 699.

 
 
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