NS  Vol.5 No.12 , December 2013
Phylogenetic relationships among small barbus, barbus (actinopterygii: Cyprinidae) from malagarasi and Pangani river basins, Tanzania
Abstract: The phylogenetic relationships among “Barbus” species (Barbus jacksonii, B. paludinosus and B. trimaculatus) from Malagarasi and Pangani River systems were examined by partial mitochondrial cytochrome b (cyt.b) gene. Sequences of the same samples from elsewhere in Africa obtained from the South African Institute of Aquatic Biodiversity (SAIAB’s) collection were also included in the analysis. Molecular technique was necessary due to phenotypic plasticity and extensive overlap of morphological features among the “Barbus species”. The findings of this study revealed that B. paludinosus from hale and Pangani populations occurred in two distinct genetic lineages though separated by a distance of approximately 10 km apart. Similar results were also noted amongst the population of B. paludinosus in Songati and Muumbara located on Lake Sagara in Malagarasi River basin. B. trimaculatus and B. jacksonii were indistinguishable such that it is envisaged that the two species are one and the same. The Barbus spp. from Africa included in the current study showed genetic affinity to neither European Barbus spp. nor South African redfins of the Pseudobarbus to conclude that the studied species of barbs are taxonomically quite different that needs further investigation to warrant renaming.
Cite this paper: Mwita, C. (2013) Phylogenetic relationships among small barbus, barbus (actinopterygii: Cyprinidae) from malagarasi and Pangani river basins, Tanzania. Natural Science, 5, 1249-1254. doi: 10.4236/ns.2013.512152.

[1]   Eccles, D.H. (1992) FAO species identification sheets for fishery purposes. Field guide to the freshwater fishes of Tanzania. Rome: UNDP Project URT/87/016.

[2]   Doadrio, I. (1990) Phylogenetic relationships and classification of west Palearctic species of the genus Barbus (Osteichthyes, Cyprinidae). Aquatic Living Resources, 3, 265-282.

[3]   Oellermann, L.K. and Skelton, P.H. (1990) Hexaploidy in yellowfish species (Barbus, Pisces, Cyprinidae) from southern Africa. Journal of Fish Biology, 37, 105-115.

[4]   Doadrio, I., Bouhandad, R. and Machordom, A. (1998) Genetic differentiation and biogeography in Saharan populations of the genus Barbus (Osteichthyes, Cyprinidae). Folia Zoology, 47, 7-20.

[5]   Turan, D., Kottelat, M. and Ekmekci, F.G. (2009) Barbus niluferensis, a new species of barbel (Teleostei: Cyprinidae) from Nilüfer River, Turkey, with re-description of B. oligolepis. Zootaxa. Turkish Journal of Fisheries and Aquatic Sciences, 1981, 15-28.

[6]   Nkotagu, H.H. and Ndaro, S.G.M. (2004) The Malagarasi wetland ecosystem: An integrated study. Dar es Salaam University Press, 141.

[7]   Zardoya, R. and Doadrio, I. (1998) Phylogenetic relationships of Iberian cyprinids: Systematic and biogeographical implications. Proceedings of the Royal Society of London, 265(B), 1365-1372.

[8]   Tamura, K., Dudley, J., Nei, M. and Kumar, S. (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Molecular Biology and Evolution, 24, 1596-1599.

[9]   Swofford, D. (2002) Phylogenetic analysis using parsimony (* and other methods) (Ver.4.0b10. ed.). Sinauer Associates, Sunderland.

[10]   Felsenstein, J. (1981) Evolutionary trees from DNA sequences: A maximum likelihood approach. Journal of Molecular Evolution, 17, 368-376.

[11]   Felsenstein, J. (1982) Numerical methods for inferring evolutionary trees. Review Biologie, 57, 1-26.

[12]   Johnson, T.C., Scholz, C.A., Talbot, M.R., Kelts, K., Ricketts, R.D., N’gobi, G. and McGill, J.W. (1996) Late Pleistocene desiccation of Lake Victoria and rapid evolution of cichlid fishes. Science, 273, 1091-1093.

[13]   Odada, E.O. and Olago, D.O. (2002) The East African Great Lakes: Limnology, palaeolimnology and biodiversity. Kluwer, London.

[14]   Stankiewiz, J. and de Wit, M.J. (2006) A proposed drainage evolution model for central Africa—Did the Congo flow East? Journal of African Earth Sciences, 44, 75-84.

[15]   Rico, C. and Turner, G.F. (2002) Extreme microallopatric divergence in a cichlid species from Lake Malawi. Molecular Ecology, 11, 1585-1590.

[16]   Salzburger, W. and Meyer, A. (2004) The species flocks of East African cichlid fishes: Recent adces in molecular phylogenetics and population genetics. Published online: 20 April Springer-Verlag. Naturwissenschaften Journal of Ichyology, 91, 277-290.

[17]   Skelton, P.H. (1993) A complete guide to the freshwater fishes of Southern Africa. Southern Book Publishers, Johannesburg.

[18]   Seegers, L. (1996) The fishes of the Lake Rukwa drainage. Tervuren, Belgium.

[19]   Greenwood, P.H. (1962) A revision of certain Barbus (Pisces: Cyprinidae) from East, Central and South Africa. Bulletin of the British Museum of natural History, 8, 151208.

[20]   Skelton, P.H. (2001) A complete guide to the freshwater fishes of southern Africa. 2nd Edition, Struik, Cape Town.